product summary
Loading...
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
DNMT3A Antibody (64B1446) - BSA Free
catalog :
NB120-13888
quantity :
0.1 mg (also 0.025 mg)
price :
459 USD
clonality :
monoclonal
host :
mouse
conjugate :
DyLight 405
clone name :
64B1446
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 124
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig 1a
Xia L, Huang W, Bellani M, Seidman M, Wu K, Fan D, et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell. 2017;31:653-668.e7 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse
Mulholland C, Smets M, Schmidtmann E, Leidescher S, Markaki Y, Hofweber M, et al. A modular open platform for systematic functional studies under physiological conditions. Nucleic Acids Res. 2015;43:e112 pubmed publisher
  • immunohistochemistry - paraffin section; human
Robaina M, Mazzoccoli L, Arruda V, Reis F, Apa A, de Rezende L, et al. Deregulation of DNMT1, DNMT3B and miR-29s in Burkitt lymphoma suggests novel contribution for disease pathogenesis. Exp Mol Pathol. 2015;98:200-7 pubmed publisher
  • western blot; mouse
Neri F, Incarnato D, Krepelova A, Rapelli S, Anselmi F, Parlato C, et al. Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics. Cell Rep. 2015;10:674-683 pubmed publisher
  • western blot; human
Guo Y, Shu L, Zhang C, Su Z, Kong A. Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1. Biochem Pharmacol. 2015;94:69-78 pubmed publisher
  • chromatin immunoprecipitation; human
Huichalaf C, Micheloni S, Ferri G, Caccia R, Gabellini D. DNA methylation analysis of the macrosatellite repeat associated with FSHD muscular dystrophy at single nucleotide level. PLoS ONE. 2014;9:e115278 pubmed publisher
  • western blot; mouse
Wang L, Zhang C, Guo Y, Su Z, Yang Y, Shu L, et al. Blocking of JB6 cell transformation by tanshinone IIA: epigenetic reactivation of Nrf2 antioxidative stress pathway. AAPS J. 2014;16:1214-25 pubmed publisher
  • immunohistochemistry - paraffin section; human
Guimarães D, Antunes D, Duarte C, Ferro L, Nunes F. DNA methyltransferase immunohistochemical expression in odontogenic tumours. J Oral Pathol Med. 2015;44:59-66 pubmed publisher
  • western blot; mouse
Paredes Gonzalez X, Fuentes F, Su Z, Kong A. Apigenin reactivates Nrf2 anti-oxidative stress signaling in mouse skin epidermal JB6 P + cells through epigenetics modifications. AAPS J. 2014;16:727-35 pubmed publisher
  • western blot; mouse
Hara S, Takano T, Ogata M, Yamakami R, Sato Y, Kono T, et al. Establishment of a conditional transgenic system using the 2A peptide in the female mouse germline. J Reprod Dev. 2014;60:250-5 pubmed
  • chromatin immunoprecipitation; human
Serra R, Fang M, Park S, Hutchinson L, Green M. A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. elife. 2014;3:e02313 pubmed publisher
  • western blot; mouse
Hara S, Takano T, Fujikawa T, Yamada M, Wakai T, Kono T, et al. Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting. Hum Mol Genet. 2014;23:3853-64 pubmed publisher
  • immunocytochemistry; human
Hara E, Ono M, Eguchi T, Kubota S, Pham H, Sonoyama W, et al. miRNA-720 controls stem cell phenotype, proliferation and differentiation of human dental pulp cells. PLoS ONE. 2013;8:e83545 pubmed publisher
  • western blot; human; fig 1
Wongtrakoongate P, Li J, Andrews P. DNMT3B inhibits the re-expression of genes associated with induced pluripotency. Exp Cell Res. 2014;321:231-9 pubmed publisher
  • western blot; mouse
Zhou J, Dhawan S, Fu H, Snyder E, Bottino R, Kundu S, et al. Combined modulation of polycomb and trithorax genes rejuvenates ? cell replication. J Clin Invest. 2013;123:4849-58 pubmed
  • immunocytochemistry; mouse
Obata Y, Wakai T, Hara S, Kono T. Long exposure to mature ooplasm can alter DNA methylation at imprinted loci in non-growing oocytes but not in prospermatogonia. Reproduction. 2014;147:H1-6 pubmed publisher
  • immunohistochemistry - frozen section; mouse
  • western blot; mouse
Kadriu B, Guidotti A, Chen Y, Grayson D. DNA methyltransferases1 (DNMT1) and 3a (DNMT3a) colocalize with GAD67-positive neurons in the GAD67-GFP mouse brain. J Comp Neurol. 2012;520:1951-64 pubmed publisher
  • immunohistochemistry; human
Yang J, Wei X, Wu Q, Xu Z, Gu D, Jin Y, et al. Clinical significance of the expression of DNA methyltransferase proteins in gastric cancer. Mol Med Rep. 2011;4:1139-43 pubmed publisher
  • western blot; mouse
Molognoni F, Cruz A, Meliso F, Morais A, Souza C, Xander P, et al. Epigenetic reprogramming as a key contributor to melanocyte malignant transformation. Epigenetics. 2011;6:450-64 pubmed
  • immunohistochemistry; mouse
Lahousse S, Hoenerhoff M, Collins J, Ton T, Masinde T, Olson D, et al. Gene expression and mutation assessment provide clues of genetic and epigenetic mechanisms in liver tumors of oxazepam-exposed mice. Vet Pathol. 2011;48:875-84 pubmed publisher
  • western blot; human
Lambrot R, Kimmins S. Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines. Int J Androl. 2011;34:110-23 pubmed publisher
  • immunocytochemistry; mouse
May A, Kirchner R, Muller H, Hartmann P, El Hajj N, Tresch A, et al. Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres. Biol Reprod. 2009;80:194-202 pubmed publisher
  • immunocytochemistry; mouse
Hirasawa R, Chiba H, Kaneda M, Tajima S, Li E, Jaenisch R, et al. Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development. Genes Dev. 2008;22:1607-16 pubmed publisher
  • western blot; human
Xia G, Schneider Stock R, Diestel A, Habold C, Krueger S, Roessner A, et al. Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation. Biochem Biophys Res Commun. 2008;369:526-31 pubmed publisher
  • western blot; mouse
Linhart H, Lin H, Yamada Y, Moran E, Steine E, Gokhale S, et al. Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev. 2007;21:3110-22 pubmed
  • western blot; mouse
Lucifero D, La Salle S, Bourc his D, Martel J, Bestor T, Trasler J. Coordinate regulation of DNA methyltransferase expression during oogenesis. BMC Dev Biol. 2007;7:36 pubmed
  • western blot; mouse
La Salle S, Trasler J. Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse. Dev Biol. 2006;296:71-82 pubmed
  • western blot; mouse
Chen T, Ueda Y, Dodge J, Wang Z, Li E. Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b. Mol Cell Biol. 2003;23:5594-605 pubmed
Hoang N, Liu Y, Bates P, Heaton A, Lopez A, Liu P, et al. Targeting DNMT3A-mediated oxidative phosphorylation to overcome ibrutinib resistance in mantle cell lymphoma. Cell Rep Med. 2024;5:101484 pubmed publisher
Brenner L, Meyer F, Yang H, K xf6 hler A, Bashtrykov P, Guo M, et al. Repeat DNA methylation is modulated by adherens junction signaling. Commun Biol. 2024;7:286 pubmed publisher
Yang C, Deng L, Bao F, Jiang H, Zhang L. Sevoflurane with Low Concentration Decrease DNA Methylation on Chronic Obstructive Pulmonary Disease (COPD)-Related Gene Promoter in COPD Rat. COPD. 2023;20:348-356 pubmed publisher
Ren G, Li H, Hong D, Hu F, Jin R, Wu S, et al. LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter. BMC Cancer. 2023;23:898 pubmed publisher
Fernandes C, da Silva R, Wood P, Ferreira M, de Almeida G, de Moraes J, et al. Titanium-Enriched Medium Promotes Environment-Induced Epigenetic Machinery Changes in Human Endothelial Cells. J Funct Biomater. 2023;14: pubmed publisher
da C Fernandes C, da Silva R, de Almeida G, Ferreira M, de Morais P, Bezerra F, et al. Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt-Chromium. J Funct Biomater. 2023;14: pubmed publisher
Narabayashi H, Koma C, Nakata K, Ikegami M, Nakanishi Y, Ogihara J, et al. Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions. Front Mol Biosci. 2022;9:1005136 pubmed publisher
Ni Q, Sun J, Wang Y, Wang Y, Liu J, Ning G, et al. mTORC1 is required for epigenetic silencing during β-cell functional maturation. Mol Metab. 2022;64:101559 pubmed publisher
Lu F, Zankharia U, Vladimirova O, Yi Y, Collman R, Lieberman P. Epigenetic Landscape of HIV-1 Infection in Primary Human Macrophage. J Virol. 2022;96:e0016222 pubmed publisher
Zhao J, Lin H, Huang K. Mesenchymal Stem Cell-derived Extracellular Vesicles Transmitting MicroRNA-34a-5p Suppress Tumorigenesis of Colorectal Cancer Through c-MYC/DNMT3a/PTEN Axis. Mol Neurobiol. 2022;59:47-60 pubmed publisher
Liu X, Cui H. The palliative effects of folic acid on retinal microvessels in diabetic retinopathy via regulating the metabolism of DNA methylation and hydroxymethylation. Bioengineered. 2021;12:10766-10774 pubmed publisher
Aronson B, Scourzic L, Shah V, Swanzey E, Kloetgen A, Polyzos A, et al. A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locus. Dev Cell. 2021;56:3052-3065.e5 pubmed publisher
da C Fernandes C, da Silva R, Fretes Wood P, Teixeira S, Bezerra F, Zambuzzi W. The molecular pathway triggered by zirconia in endothelial cells involves epigenetic control. Tissue Cell. 2021;73:101627 pubmed publisher
Kim K, Li C, Bunina D, Jeong H, Ghelman J, Yoon J, et al. Donor cell memory confers a metastable state of directly converted cells. Cell Stem Cell. 2021;28:1291-1306.e10 pubmed publisher
Huang Z, Yu J, Cui W, Johnson B, Kim K, Pfeifer G. The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins. Sci Adv. 2021;7: pubmed publisher
Broche J, Kungulovski G, Bashtrykov P, Rathert P, Jeltsch A. Genome-wide investigation of the dynamic changes of epigenome modifications after global DNA methylation editing. Nucleic Acids Res. 2021;49:158-176 pubmed publisher
Wei J, Cheng J, Waddell N, Wang Z, Pang X, Cao Q, et al. DNA Methyltransferase 3A Is Involved in the Sustained Effects of Chronic Stress on Synaptic Functions and Behaviors. Cereb Cortex. 2021;31:1998-2012 pubmed publisher
Cárdenas H, Fang F, Jiang G, Perkins S, Zhang C, Emerson R, et al. Methylomic Signatures of High Grade Serous Ovarian Cancer. Epigenetics. 2020;:1-16 pubmed publisher
Zaro B, Noh J, Mascetti V, Demeter J, George B, Zukowska M, et al. Proteomic analysis of young and old mouse hematopoietic stem cells and their progenitors reveals post-transcriptional regulation in stem cells. elife. 2020;9: pubmed publisher
Jia Y, Xie H, Zhang J, Ying H. Induction of TGF-β receptor I expression in a DNA methylation-independent manner mediated by DNMT3A downregulation is involved in early-onset severe preeclampsia. FASEB J. 2020;34:13224-13238 pubmed publisher
Magno Guimarães D, deLucas da Silva Almeida F, Moraes Castilho R, Eduardo Nor J, Daumas Nunes F. DNA methyltransferase expression is associated with cell proliferation in salivary mucoepidermoid carcinoma. J Oral Pathol Med. 2020;49:1053-1060 pubmed publisher
Yagi M, Kabata M, Tanaka A, Ukai T, Ohta S, Nakabayashi K, et al. Identification of distinct loci for de novo DNA methylation by DNMT3A and DNMT3B during mammalian development. Nat Commun. 2020;11:3199 pubmed publisher
Dahlet T, Argüeso Lleida A, Al Adhami H, Dumas M, Bender A, Ngondo R, et al. Genome-wide analysis in the mouse embryo reveals the importance of DNA methylation for transcription integrity. Nat Commun. 2020;11:3153 pubmed publisher
Ginno P, Gaidatzis D, Feldmann A, Hoerner L, Imanci D, Burger L, et al. A genome-scale map of DNA methylation turnover identifies site-specific dependencies of DNMT and TET activity. Nat Commun. 2020;11:2680 pubmed publisher
McAninch D, Mäkelä J, La H, Hughes J, Lovell Badge R, Hobbs R, et al. SOX3 promotes generation of committed spermatogonia in postnatal mouse testes. Sci Rep. 2020;10:6751 pubmed publisher
Lavery L, Ure K, Wan Y, Luo C, Trostle A, Wang W, et al. Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome. elife. 2020;9: pubmed publisher
Ravichandran M, Lei R, Tang Q, Zhao Y, Lee J, Ma L, et al. Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells. Cell Rep. 2019;28:1993-2003.e5 pubmed publisher
Kong X, Chen J, Xie W, Brown S, Cai Y, Wu K, et al. Defining UHRF1 Domains that Support Maintenance of Human Colon Cancer DNA Methylation and Oncogenic Properties. Cancer Cell. 2019;35:633-648.e7 pubmed publisher
Heyn P, Logan C, Fluteau A, Challis R, Auchynnikava T, Martin C, et al. Gain-of-function DNMT3A mutations cause microcephalic dwarfism and hypermethylation of Polycomb-regulated regions. Nat Genet. 2019;51:96-105 pubmed publisher
van Mierlo G, Dirks R, De Clerck L, Brinkman A, Huth M, Kloet S, et al. Integrative Proteomic Profiling Reveals PRC2-Dependent Epigenetic Crosstalk Maintains Ground-State Pluripotency. Cell Stem Cell. 2019;24:123-137.e8 pubmed publisher
Cyphert H, Walker E, Hang Y, Dhawan S, Haliyur R, Bonatakis L, et al. Examining How the MAFB Transcription Factor Affects Islet β-Cell Function Postnatally. Diabetes. 2019;68:337-348 pubmed publisher
Pang Y, Liu J, Li X, Xiao G, Wang H, Yang G, et al. MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer. J Cell Mol Med. 2018;22:6262-6274 pubmed publisher
Chatterjee B, Lin M, Chen C, Peng K, Wu M, Tseng M, et al. DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells. Biochim Biophys Acta Gene Regul Mech. 2018;1861:1048-1061 pubmed publisher
La H, Mäkelä J, Chan A, Rossello F, Nefzger C, Legrand J, et al. Identification of dynamic undifferentiated cell states within the male germline. Nat Commun. 2018;9:2819 pubmed publisher
Yagi M, Kishigami S, Tanaka A, Semi K, Mizutani E, Wakayama S, et al. Derivation of ground-state female ES cells maintaining gamete-derived DNA methylation. Nature. 2017;548:224-227 pubmed publisher
Sheikh M, Malik Y, Zhu X. RA-Induced Transcriptional Silencing of Checkpoint Kinase-2 through Promoter Methylation by Dnmt3b Is Required for Neuronal Differentiation of P19 Cells. J Mol Biol. 2017;429:2463-2473 pubmed publisher
Ni Q, Gu Y, Xie Y, Yin Q, Zhang H, Nie A, et al. Raptor regulates functional maturation of murine beta cells. Nat Commun. 2017;8:15755 pubmed publisher
Mahadevan S, Sathappan V, Utama B, Lorenzo I, Kaskar K, Van den Veyver I. Maternally expressed NLRP2 links the subcortical maternal complex (SCMC) to fertility, embryogenesis and epigenetic reprogramming. Sci Rep. 2017;7:44667 pubmed publisher
Neri F, Rapelli S, Krepelova A, Incarnato D, Parlato C, Basile G, et al. Intragenic DNA methylation prevents spurious transcription initiation. Nature. 2017;543:72-77 pubmed publisher
Acharya M, Baddour A, Kawashita T, Allen B, Syage A, Nguyen T, et al. Epigenetic determinants of space radiation-induced cognitive dysfunction. Sci Rep. 2017;7:42885 pubmed publisher
Thakur A, Mackin S, Irwin R, O Neill K, Pollin G, Walsh C. Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin. 2016;9:53 pubmed
Hoang T, Horowitz E, Chaffee B, Qi P, Flake R, Bruney D, et al. Lens development requires DNMT1 but takes place normally in the absence of both DNMT3A and DNMT3B activity. Epigenetics. 2017;12:27-40 pubmed publisher
Daniel F, Alves S, Vieira D, Biz M, Daniel I, Modolo F. Immunohistochemical expression of DNA methyltransferases 1, 3a, and 3b in actinic cheilitis and lip squamous cell carcinomas. J Oral Pathol Med. 2016;45:774-779 pubmed publisher
Xu X, Tan X, Hulshoff M, Wilhelmi T, Zeisberg M, Zeisberg E. Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells. FEBS Lett. 2016;590:1222-33 pubmed publisher
Hong B, Su Z, Zhang C, Yang Y, Guo Y, Li W, et al. Reserpine Inhibit the JB6 P+ Cell Transformation Through Epigenetic Reactivation of Nrf2-Mediated Anti-oxidative Stress Pathway. AAPS J. 2016;18:659-69 pubmed publisher
Dhawan S, Dirice E, Kulkarni R, Bhushan A. Inhibition of TGF-β Signaling Promotes Human Pancreatic β-Cell Replication. Diabetes. 2016;65:1208-18 pubmed publisher
Amouroux R, Nashun B, Shirane K, Nakagawa S, Hill P, D Souza Z, et al. De novo DNA methylation drives 5hmC accumulation in mouse zygotes. Nat Cell Biol. 2016;18:225-233 pubmed publisher
Li Y, Seah M, O NEILL C. Mapping global changes in nuclear cytosine base modifications in the early mouse embryo. Reproduction. 2016;151:83-95 pubmed publisher
Kita H, Okamoto K, Kushima R, Kawauchi A, Chano T. Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas. Oncol Lett. 2015;10:661-666 pubmed
Tsukada Y, Akiyama T, Nakayama K. Maternal TET3 is dispensable for embryonic development but is required for neonatal growth. Sci Rep. 2015;5:15876 pubmed publisher
Dhawan S, Tschen S, Zeng C, Guo T, Hebrok M, MATVEYENKO A, et al. DNA methylation directs functional maturation of pancreatic β cells. J Clin Invest. 2015;125:2851-60 pubmed publisher
Emperle M, Rajavelu A, Reinhardt R, Jurkowska R, Jeltsch A. Cooperative DNA binding and protein/DNA fiber formation increases the activity of the Dnmt3a DNA methyltransferase. J Biol Chem. 2014;289:29602-13 pubmed publisher
Chuang C, Wang W, Li C, Ko C, Chou Y, Chuu C, et al. The combination of the prodrugs perforin-CEBPD and perforin-granzyme B efficiently enhances the activation of caspase signaling and kills prostate cancer. Cell Death Dis. 2014;5:e1220 pubmed publisher
Takahashi M, Kamei Y, Ehara T, Yuan X, Suganami T, Takai Igarashi T, et al. Analysis of DNA methylation change induced by Dnmt3b in mouse hepatocytes. Biochem Biophys Res Commun. 2013;434:873-8 pubmed publisher
Park E, Park Y, Shin C, Park S, Ahn S, Kim D, et al. Hepatitis B virus inhibits liver regeneration via epigenetic regulation of urokinase-type plasminogen activator. Hepatology. 2013;58:762-76 pubmed publisher
Leitch H, McEwen K, Turp A, Encheva V, Carroll T, Grabole N, et al. Naive pluripotency is associated with global DNA hypomethylation. Nat Struct Mol Biol. 2013;20:311-6 pubmed publisher
Sheikh M, Malik Y, Yu H, Lai M, Wang X, Zhu X. Epigenetic regulation of Dpp6 expression by Dnmt3b and its novel role in the inhibition of RA induced neuronal differentiation of P19 cells. PLoS ONE. 2013;8:e55826 pubmed publisher
Zimmermann N, Zschocke J, Perisic T, Yu S, Holsboer F, Rein T. Antidepressants inhibit DNA methyltransferase 1 through reducing G9a levels. Biochem J. 2012;448:93-102 pubmed publisher
Hong G, Bain L. Sodium arsenite represses the expression of myogenin in C2C12 mouse myoblast cells through histone modifications and altered expression of Ezh2, Glp, and Igf-1. Toxicol Appl Pharmacol. 2012;260:250-9 pubmed publisher
Stadtfeld M, Apostolou E, Ferrari F, Choi J, Walsh R, Chen T, et al. Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells. Nat Genet. 2012;44:398-405, S1-2 pubmed publisher
Selvakumar T, Gjidoda A, Hovde S, Henry R. Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases. J Biol Chem. 2012;287:7039-50 pubmed publisher
Kurdíková V, Smolinský R, Gvoždík L. Mothers matter too: benefits of temperature oviposition preferences in newts. PLoS ONE. 2011;6:e23842 pubmed publisher
Pawlak M, Jaenisch R. De novo DNA methylation by Dnmt3a and Dnmt3b is dispensable for nuclear reprogramming of somatic cells to a pluripotent state. Genes Dev. 2011;25:1035-40 pubmed publisher
Nasonkin I, Lazo K, Hambright D, Brooks M, Fariss R, Swaroop A. Distinct nuclear localization patterns of DNA methyltransferases in developing and mature mammalian retina. J Comp Neurol. 2011;519:1914-30 pubmed publisher
Daniel F, Rivero E, Modolo F, Lopes T, Salum F. Immunohistochemical expression of DNA methyltransferases 1, 3a and 3b in oral leukoplakias and squamous cell carcinomas. Arch Oral Biol. 2010;55:1024-30 pubmed publisher
Kashiwagi K, Nimura K, Ura K, Kaneda Y. DNA methyltransferase 3b preferentially associates with condensed chromatin. Nucleic Acids Res. 2011;39:874-88 pubmed publisher
Pierard V, Guiguen A, Colin L, Wijmeersch G, Vanhulle C, Van Driessche B, et al. DNA cytosine methylation in the bovine leukemia virus promoter is associated with latency in a lymphoma-derived B-cell line: potential involvement of direct inhibition of cAMP-responsive element (CRE)-binding protein/CRE modulator/activation transcri. J Biol Chem. 2010;285:19434-49 pubmed publisher
Wu Y, Alvarez M, Slamon D, Koeffler P, Vadgama J. Caspase 8 and maspin are downregulated in breast cancer cells due to CpG site promoter methylation. BMC Cancer. 2010;10:32 pubmed publisher
Fan H, Liu D, Qiu X, Qiao F, Wu Q, Su X, et al. A functional polymorphism in the DNA methyltransferase-3A promoter modifies the susceptibility in gastric cancer but not in esophageal carcinoma. BMC Med. 2010;8:12 pubmed publisher
Meilinger D, Fellinger K, Bultmann S, Rothbauer U, Bonapace I, Klinkert W, et al. Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells. EMBO Rep. 2009;10:1259-64 pubmed publisher
Ko C, Hsu H, Shen M, Chang W, Wang J. Epigenetic silencing of CCAAT/enhancer-binding protein delta activity by YY1/polycomb group/DNA methyltransferase complex. J Biol Chem. 2008;283:30919-32 pubmed publisher
Zhu F, Xia X, Liu B, Shen J, Hu Y, Person M, et al. IKKalpha shields 14-3-3sigma, a G(2)/M cell cycle checkpoint gene, from hypermethylation, preventing its silencing. Mol Cell. 2007;27:214-27 pubmed
Oh B, Kim H, Park H, Shim Y, Choi J, Park C, et al. DNA methyltransferase expression and DNA methylation in human hepatocellular carcinoma and their clinicopathological correlation. Int J Mol Med. 2007;20:65-73 pubmed
Tadokoro Y, Ema H, Okano M, Li E, Nakauchi H. De novo DNA methyltransferase is essential for self-renewal, but not for differentiation, in hematopoietic stem cells. J Exp Med. 2007;204:715-22 pubmed
Park I, Sohn B, Yu E, Suh D, Chung Y, Lee J, et al. Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein. Gastroenterology. 2007;132:1476-94 pubmed
Spada F, Haemmer A, Kuch D, Rothbauer U, Schermelleh L, Kremmer E, et al. DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells. J Cell Biol. 2007;176:565-71 pubmed
Nimura K, Ishida C, Koriyama H, Hata K, Yamanaka S, Li E, et al. Dnmt3a2 targets endogenous Dnmt3L to ES cell chromatin and induces regional DNA methylation. Genes Cells. 2006;11:1225-37 pubmed
Shen Y, Chow J, Wang Z, Fan G. Abnormal CpG island methylation occurs during in vitro differentiation of human embryonic stem cells. Hum Mol Genet. 2006;15:2623-35 pubmed
Iwatani M, Ikegami K, Kremenska Y, Hattori N, Tanaka S, Yagi S, et al. Dimethyl sulfoxide has an impact on epigenetic profile in mouse embryoid body. Stem Cells. 2006;24:2549-56 pubmed
Tsumura A, Hayakawa T, Kumaki Y, Takebayashi S, Sakaue M, Matsuoka C, et al. Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Genes Cells. 2006;11:805-14 pubmed
Jones B, Chen J. Inhibition of IFN-gamma transcription by site-specific methylation during T helper cell development. EMBO J. 2006;25:2443-52 pubmed
Ueda Y, Okano M, Williams C, Chen T, Georgopoulos K, Li E. Roles for Dnmt3b in mammalian development: a mouse model for the ICF syndrome. Development. 2006;133:1183-92 pubmed
Galetzka D, Tralau T, Stein R, Haaf T. Expression of DNMT3A transcripts and nucleolar localization of DNMT3A protein in human testicular and fibroblast cells suggest a role for de novo DNA methylation in nucleolar inactivation. J Cell Biochem. 2006;98:885-94 pubmed
MacDonald J, Gin C, Roskams A. Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development. Dev Biol. 2005;288:461-73 pubmed
Qiu Y, Mirkin B, Dwivedi R. Inhibition of DNA methyltransferase reverses cisplatin induced drug resistance in murine neuroblastoma cells. Cancer Detect Prev. 2005;29:456-63 pubmed
Park H, Yu E, Shim Y. DNA methyltransferase expression and DNA hypermethylation in human hepatocellular carcinoma. Cancer Lett. 2006;233:271-8 pubmed
Oka M, Meacham A, Hamazaki T, Rodic N, Chang L, Terada N. De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine. Oncogene. 2005;24:3091-9 pubmed
Stempak J, Sohn K, Chiang E, Shane B, Kim Y. Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model. Carcinogenesis. 2005;26:981-90 pubmed
Feng J, Chang H, Li E, Fan G. Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system. J Neurosci Res. 2005;79:734-46 pubmed
Leu Y, Yan P, Fan M, Jin V, Liu J, Curran E, et al. Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer. Cancer Res. 2004;64:8184-92 pubmed
Park I, Sohn B, Choo J, Joe C, Seong J, Lee Y, et al. Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development. J Cell Biochem. 2005;94:585-96 pubmed
Weisenberger D, Velicescu M, Cheng J, Gonzales F, Liang G, Jones P. Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation. Mol Cancer Res. 2004;2:62-72 pubmed
Yan Q, Cho E, Lockett S, Muegge K. Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin. Mol Cell Biol. 2003;23:8416-28 pubmed
Choi M, Shim Y, Hwa J, Lee S, Ro J, Kim J, et al. Expression of DNA methyltransferases in multistep hepatocarcinogenesis. Hum Pathol. 2003;34:11-7 pubmed
Chen T, Ueda Y, Xie S, Li E. A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J Biol Chem. 2002;277:38746-54 pubmed
Dennis K, Fan T, Geiman T, Yan Q, Muegge K. Lsh, a member of the SNF2 family, is required for genome-wide methylation. Genes Dev. 2001;15:2940-4 pubmed
product information
brand :
Novus Biologicals, a Bio-Techne brand
catalog number base :
NB120-13888
SKU :
NB120-13888
product name :
DNMT3A Antibody (64B1446) - BSA Free
description :
The DNMT3A Antibody (64B1446) - BSA Free from Novus Biologicals is a mouse monoclonal antibody to DNMT3A. This antibody reacts with human,mouse,rat. The DNMT3A Antibody (64B1446) - BSA Free has been validated for the following applications: Chromatin Immunoprecipitation,Flow Cytometry,CyTOF-ready,Knockdown Validated,Chemotaxis,IF/IHC,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunohistochemistry Whole-Mount,Chromatin Immunoprecipitation (ChIP),Knockout Validated,Immunocytochemistry/ Immunofluorescence.
target :
DNMT3A
unit size :
0.1 mg (also 0.025 mg)
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
64B1446
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
This DNMT3A Antibody (64B1446) was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
isotype :
IgG1 Kappa
purity :
Protein G purified
species :
Human,Mouse,Rat
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
gene symbol :
DNMT3A
Antibody validation :
Biological Validation
applications :
Chromatin Immunoprecipitation,Flow Cytometry,CyTOF-ready,Knockdown Validated,Chemotaxis,IF/IHC,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunohistochemistry Whole-Mount,Chromatin Immunoprecipitation (ChIP),Knockout Validated,Immunocytochemistry/ Immunofluorescence
USD :
459
USD 2025 :
459 USD
alt names :
64B1446 clone, DNA (cytosine-5-)-methyltransferase 3 alpha, DNA (cytosine-5)-methyltransferase 3A, DNA cytosine methyltransferase 3A2, DNA Methyltransferase 3 Alpha, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, Dnmt3a, DNMT3A2, EC 2.1.1.37, M.HsaIIIA, TBRS
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.