product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free
catalog :
NB110-93479
quantity :
0.05 ml (also 0.025 ml)
price :
409 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
FITC
clone name :
KBB36
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 47
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 2a, 3b
Siu M, Jiang Y, Wang J, Leung T, Ngu S, Cheung A, et al. PDK1 promotes ovarian cancer metastasis by modulating tumor-mesothelial adhesion, invasion, and angiogenesis via α5β1 integrin and JNK/IL-8 signaling. Oncogenesis. 2020;9:24 pubmed publisher
  • western blot; human; 1:1000; fig s4e
Chhipa R, Fan Q, Anderson J, Muraleedharan R, Huang Y, Ciraolo G, et al. AMP kinase promotes glioblastoma bioenergetics and tumour growth. Nat Cell Biol. 2018;20:823-835 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5a
Zhong W, Yi Q, Xu B, Li S, Wang T, Liu F, et al. ORP4L is essential for T-cell acute lymphoblastic leukemia cell survival. Nat Commun. 2016;7:12702 pubmed publisher
Li Y, Cai J, Liu Y, Li C, Chen X, Wong W, et al. CcpA-Knockout Staphylococcus aureus Induces Abnormal Metabolic Phenotype via the Activation of Hepatic STAT5/PDK4 Signaling in Diabetic Mice. Pathogens. 2023;12: pubmed publisher
Huo J, Prasad V, Grimes K, Vanhoutte D, Blair N, Lin S, et al. MCUb is an inducible regulator of calcium-dependent mitochondrial metabolism and substrate utilization in muscle. Cell Rep. 2023;42:113465 pubmed publisher
Rossi S, Matzkin M, Riviere E, Martinez G, Ponzio R, Levalle O, et al. Melatonin improves oxidative state and lactate metabolism in rodent Sertoli cells. Mol Cell Endocrinol. 2023;576:112034 pubmed publisher
Bekeova C, Han J, Xu H, Kerr E, Blackburne B, Lynch S, et al. Acyl-CoA thioesterase-2 facilitates β-oxidation in glycolytic skeletal muscle in a lipid supply dependent manner. bioRxiv. 2023;: pubmed publisher
Li J, Zhu X, Oberdier M, Lee C, Lin S, Fink S, et al. A cell-penetrating PHLPP peptide improves cardiac arrest survival in murine and swine models. J Clin Invest. 2023;133: pubmed publisher
HOLLOWAY C, Zhong G, Kim Y, Ye H, Sampath H, Hammerling U, et al. Retinoic acid regulates pyruvate dehydrogenase kinase 4 (Pdk4) to modulate fuel utilization in the adult heart: Insights from wild-type and β-carotene 9',10' oxygenase knockout mice. FASEB J. 2022;36:e22513 pubmed publisher
Ito N, Takatsu A, Ito H, Koike Y, Yoshioka K, Kamei Y, et al. Slc12a8 in the lateral hypothalamus maintains energy metabolism and skeletal muscle functions during aging. Cell Rep. 2022;40:111131 pubmed publisher
Li J, Chang W, Qin G, Wojcik K, Li C, Hsu C, et al. Baicalein Preconditioning Cardioprotection Involves Pro-Oxidant Signaling and Activation of Pyruvate Dehydrogenase. Am J Chin Med. 2022;50:1255-1267 pubmed publisher
Martin A, Fernandez M, Cattaneo E, Schuster C, Venara M, Cl xe9 ment F, et al. Type 1 Insulin-Like Growth Factor Receptor Nuclear Localization in High-Grade Glioma Cells Enhances Motility, Metabolism, and In Vivo Tumorigenesis. Front Endocrinol (Lausanne). 2022;13:849279 pubmed publisher
Gatie M, Cooper T, Khazaee R, Lajoie G, Kelly G. Lactate Enhances Mouse ES Cell Differentiation Toward XEN Cells In Vitro. Stem Cells. 2022;40:239-259 pubmed publisher
Damerau A, Kirchner M, Pfeiffenberger M, Ehlers L, Do Nguyen D, Mertins P, et al. Metabolic reprogramming of synovial fibroblasts in osteoarthritis by inhibition of pathologically overexpressed pyruvate dehydrogenase kinases. Metab Eng. 2022;72:116-132 pubmed publisher
Kanan Y, Hackett S, Taneja K, Khan M, Campochiaro P. Oxidative stress-induced alterations in retinal glucose metabolism in Retinitis Pigmentosa. Free Radic Biol Med. 2022;181:143-153 pubmed publisher
Burns J, Hurst C, Knowles M, Phillips R, Allison S. The Warburg effect as a therapeutic target for bladder cancers and intratumoral heterogeneity in associated molecular targets. Cancer Sci. 2021;112:3822-3834 pubmed publisher
Vitvitsky V, Kumar R, Libiad M, Maebius A, Landry A, Banerjee R. The mitochondrial NADH pool is involved in hydrogen sulfide signaling and stimulation of aerobic glycolysis. J Biol Chem. 2021;296:100736 pubmed publisher
Santiappillai N, Abuhammad S, Slater A, Kirby L, McArthur G, Sheppard K, et al. CDK4/6 Inhibition Reprograms Mitochondrial Metabolism in BRAFV600 Melanoma via a p53 Dependent Pathway. Cancers (Basel). 2021;13: pubmed publisher
Hjelholt A, Charidemou E, Griffin J, Pedersen S, Gudiksen A, Pilegaard H, et al. Insulin resistance induced by growth hormone is linked to lipolysis and associated with suppressed pyruvate dehydrogenase activity in skeletal muscle: a 2 × 2 factorial, randomised, crossover study in human individuals. Diabetologia. 2020;63:2641-2653 pubmed publisher
Na Y, Jung D, Song J, Park J, Seok S. Pyruvate dehydrogenase kinase is a negative regulator of interleukin-10 production in macrophages. J Mol Cell Biol. 2020;: pubmed publisher
Jebessa Z, Shanmukha Kumar D, Dewenter M, Lehmann L, Xu C, Schreiter F, et al. The lipid droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4. Nat Metab. 2019;1:1157-1167 pubmed publisher
Lu H, Lu Y, Xie Y, Qiu S, Li X, Fan Z. Rational combination with PDK1 inhibition overcomes cetuximab resistance in head and neck squamous cell carcinoma. JCI Insight. 2019;4: pubmed publisher
Jiang H, Jin J, Duan Y, Xie Z, Li Y, Gao A, et al. Mitochondrial Uncoupling Coordinated With PDH Activation Safely Ameliorates Hyperglycemia via Promoting Glucose Oxidation. Diabetes. 2019;68:2197-2209 pubmed publisher
Li J, Zhu X, Wang H, Lee C, Chen S, Qian Y, et al. Akt1-mediated CPR cooling protection targets regulators of metabolism, inflammation and contractile function in mouse cardiac arrest. PLoS ONE. 2019;14:e0220604 pubmed publisher
Thapa D, Zhang M, Manning J, Guimaraes D, Stoner M, Lai Y, et al. Loss of GCN5L1 in cardiac cells limits mitochondrial respiratory capacity under hyperglycemic conditions. Physiol Rep. 2019;7:e14054 pubmed publisher
Zhong W, Xu M, Li C, Zhu B, Cao X, Li D, et al. ORP4L Extracts and Presents PIP2 from Plasma Membrane for PLCβ3 Catalysis: Targeting It Eradicates Leukemia Stem Cells. Cell Rep. 2019;26:2166-2177.e9 pubmed publisher
Zhuang Y, Ly R, Frazier C, Yu J, Qin S, Fan X, et al. The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer. Cancer Metab. 2019;7:1 pubmed publisher
Kwong J, Huo J, Bround M, Boyer J, Schwanekamp J, Ghazal N, et al. The mitochondrial calcium uniporter underlies metabolic fuel preference in skeletal muscle. JCI Insight. 2018;3: pubmed publisher
Deng Y, Deng Z, Hao H, Wu X, Gao H, Tang S, et al. MicroRNA-23a promotes colorectal cancer cell survival by targeting PDK4. Exp Cell Res. 2018;373:171-179 pubmed publisher
Thapa D, Wu K, Stoner M, Xie B, Zhang M, Manning J, et al. The protein acetylase GCN5L1 modulates hepatic fatty acid oxidation activity via acetylation of the mitochondrial β-oxidation enzyme HADHA. J Biol Chem. 2018;293:17676-17684 pubmed publisher
Lemecha M, Morino K, Imamura T, Iwasaki H, Ohashi N, Ida S, et al. MiR-494-3p regulates mitochondrial biogenesis and thermogenesis through PGC1-α signalling in beige adipocytes. Sci Rep. 2018;8:15096 pubmed publisher
Gao Y, Zhou Z, Ren T, Kim S, He Y, Seo W, et al. Alcohol inhibits T-cell glucose metabolism and hepatitis in ALDH2-deficient mice and humans: roles of acetaldehyde and glucocorticoids. Gut. 2019;68:1311-1322 pubmed publisher
Menk A, Scharping N, Moreci R, Zeng X, Guy C, Salvatore S, et al. Early TCR Signaling Induces Rapid Aerobic Glycolysis Enabling Distinct Acute T Cell Effector Functions. Cell Rep. 2018;22:1509-1521 pubmed publisher
Xu S, Liu C, Xu W, Huang L, Zhao J, Zhao S. Butyrate induces apoptosis by activating PDC and inhibiting complex I through SIRT3 inactivation. Signal Transduct Target Ther. 2017;2:16035 pubmed publisher
Liu X, Zuo R, Bao Y, Qu X, Sun K, Ying H. Down-regulation of PDK4 is Critical for the Switch of Carbohydrate Catabolism during Syncytialization of Human Placental Trophoblasts. Sci Rep. 2017;7:8474 pubmed publisher
Gorga A, Rindone G, Regueira M, Pellizzari E, Camberos M, Cigorraga S, et al. PPARγ activation regulates lipid droplet formation and lactate production in rat Sertoli cells. Cell Tissue Res. 2017;369:611-624 pubmed publisher
Bhosale G, Sharpe J, Koh A, Kouli A, Szabadkai G, Duchen M. Pathological consequences of MICU1 mutations on mitochondrial calcium signalling and bioenergetics. Biochim Biophys Acta Mol Cell Res. 2017;1864:1009-1017 pubmed publisher
Jun J, Devera R, Unnikrishnan D, Shin M, Bevans Fonti S, Yao Q, et al. Adipose HIF-1α causes obesity by suppressing brown adipose tissue thermogenesis. J Mol Med (Berl). 2017;95:287-297 pubmed publisher
Lu H, Li X, Lu Y, Qiu S, Fan Z. ASCT2 (SLC1A5) is an EGFR-associated protein that can be co-targeted by cetuximab to sensitize cancer cells to ROS-induced apoptosis. Cancer Lett. 2016;381:23-30 pubmed publisher
Rossi S, Windschüttl S, Matzkin M, Rey Ares V, Terradas C, Ponzio R, et al. Reactive oxygen species (ROS) production triggered by prostaglandin D2 (PGD2) regulates lactate dehydrogenase (LDH) expression/activity in TM4 Sertoli cells. Mol Cell Endocrinol. 2016;434:154-65 pubmed publisher
Regueira M, Artagaveytia S, Galardo M, Pellizzari E, Cigorraga S, Meroni S, et al. Novel molecular mechanisms involved in hormonal regulation of lactate production in Sertoli cells. Reproduction. 2015;150:311-21 pubmed publisher
Zuo R, Gu X, Qi Q, Wang T, Zhao X, Liu J, et al. Warburg-like Glycolysis and Lactate Shuttle in Mouse Decidua during Early Pregnancy. J Biol Chem. 2015;290:21280-91 pubmed publisher
Hagopian K, Tomilov A, Kim K, Cortopassi G, Ramsey J. Key glycolytic enzyme activities of skeletal muscle are decreased under fed and fasted states in mice with knocked down levels of Shc proteins. PLoS ONE. 2015;10:e0124204 pubmed publisher
Llorente Folch I, Rueda C, Pardo B, Szabadkai G, Duchen M, Satrustegui J. The regulation of neuronal mitochondrial metabolism by calcium. J Physiol. 2015;593:3447-62 pubmed publisher
Rellinger E, Romain C, Choi S, Qiao J, Chung D. Silencing gastrin-releasing peptide receptor suppresses key regulators of aerobic glycolysis in neuroblastoma cells. Pediatr Blood Cancer. 2015;62:581-6 pubmed publisher
Ho N, Coomber B. Pyruvate dehydrogenase kinase expression and metabolic changes following dichloroacetate exposure in anoxic human colorectal cancer cells. Exp Cell Res. 2015;331:73-81 pubmed publisher
Delaney L, Ho N, Morrison J, Farias N, Mosser D, Coomber B. Dichloroacetate affects proliferation but not survival of human colorectal cancer cells. Apoptosis. 2015;20:63-74 pubmed publisher
product information
master code :
NB110-93479
SKU :
NB110-93479
product name :
Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free
unit size :
0.05 ml (also 0.025 ml)
description :
The Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free from Novus is a rabbit polyclonal antibody to Pyruvate Dehydrogenase E1-alpha subunit. This antibody reacts with human,mouse,rat. The Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free has been validated for the following applications: Simple Western,IF/IHC,Flow Cytometry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Immunoblotting,Immunohistochemistry Free-Floating,Knockout Validated,Immunocytochemistry/ Immunofluorescence.
target :
Pyruvate Dehydrogenase E1-alpha subunit
category :
Primary Antibodies
buffer :
PBS (pH 7.4)
clonality :
Polyclonal
concentration :
1 mg/ml
conjugate :
Unconjugated
host :
Rabbit
immunogen :
A synthetic peptide surrounding the phosphorylated serine 293 of the human Pyruvate Dehydrogenase E1-alpha subunit protein. [Swiss-Prot: #P08559]
isotype :
IgG
purity :
Immunogen affinity purified
species :
Human,Mouse,Rat
specificity :
This is specific for the phosphorylated Serine 293 form of the PDHE1 alpha protein
theoretical molecular weight :
43 kDa
gene symbol :
PDHA1
Antibody validation :
Biological Validation
applications :
Simple Western,IF/IHC,Flow Cytometry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Immunoblotting,Immunohistochemistry Free-Floating,Knockout Validated,Immunocytochemistry/ Immunofluorescence
USD :
409 USD
alt names :
EC 1.2.4.1, PDHA, PDHE1-A type I, PHE1APDHCE1A, pyruvate dehydrogenase (lipoamide) alpha 1, pyruvate dehydrogenase complex, E1-alpha polypeptide 1, pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.