product summary
request information :
company name :
Novus Biologicals
product type :
antibody
product name :
Rabbit Polyclonal CD11b Antibody
catalog :
NB110-89474G
quantity :
0.1 ml
price :
399.00 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human, cow, rat, mouse
application :
western blot
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB110-89474G
Product Name :
Rabbit Polyclonal CD11b Antibody
List Price :
399.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
antigen CD11b (p170), CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide), CD11b, CD11b antigen, CD11 antigen-like family member B, Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, CR3AMGC117044, integrin alpha-M, integrin, alpha M (complement component 3 receptor 3 subunit), Leukocyte adhesion receptor MO1, macrophage antigen alpha polypeptide, MAC1A, MAC-1, MO1A, Neutrophil adherence receptor, neutrophil adherence receptor alpha-M subunit, SLEB6
Background :
CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
Buffer :
50mM sodium borate
Clonality :
Polyclonal
Concentration :
1.0 mg/ml
Conjugate :
DyLight 488
Application Summary :
Western Blot
Host :
Rabbit
Immunogen :
A synthetic peptide made to an internal region (within residues 250-350) of the mouse CD11b protein. [Swiss-Prot# P05555]
Localization :
Monocytes and granulocytes.
Marker :
Microglia Marker
Preservative :
No Preservative
Purity :
Immunogen affinity purified
CrossReactivity :
Mouse, rat and human. We have feedback that this antibody does not work in human samples with Western blot. Immunogen has 100% homology to bovine and 88% homology with sheep.
Storage :
Store at 4C in the dark.
Swissprot Human :
P11215
TMW :
160 kDa
Uses :
This CD11b antibody is useful for Western blot analysis, Immunocytochemistry, Flow Cytometry (PMID 21422470) and IHC-paraffin embedded sections. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human with Western blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916)
Research Area :
Cancer, Breast Cancer, CD Markers, Cellular Markers, Cytoskeleton, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Microglia Markers, Myeloid Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: usa
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.