NHE3/SLC9A3 [p Ser552] Antibody (14D5) - BSA Free | Novus Biologicals NB110-81529 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

NHE3/SLC9A3 [p Ser552] Antibody (14D5) - BSA Free

catalog :

NB110-81529

quantity :

0.1 ml (also 0.025 ml)

price :

409 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

14D5

reactivity :

primate, human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Novus Biologicals product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human immunocytochemistry; human western blot; human	Ozkucur N, Song B, Bola S, Zhang L, Reid B, Fu G, et al. NHE3 phosphorylation via PKC? marks the polarity and orientation of directionally migrating cells. Cell Mol Life Sci. 2014;71:4653-4663 pubmed publisher
immunoprecipitation; human western blot; human	Perike S, Ozkucur N, Sharma P, Staroske W, Bläsche R, Barth K, et al. Phospho-NHE3 forms membrane patches and interacts with beta-actin to sense and maintain constant direction during cell migration. Exp Cell Res. 2014;324:13-29 pubmed publisher
western blot; human	Liu T, Jose P. Gastrin induces sodium-hydrogen exchanger 3 phosphorylation and mTOR activation via a phosphoinositide 3-kinase-/protein kinase C-dependent but AKT-independent pathway in renal proximal tubule cells derived from a normotensive male human. Endocrinology. 2013;154:865-75 pubmed publisher
western blot; mouse	Rieg T, Gerasimova M, Murray F, Masuda T, Tang T, Rose M, et al. Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice. Am J Physiol Renal Physiol. 2012;303:F963-71 pubmed publisher
flow cytometry; human immunoprecipitation; human immunocytochemistry; human western blot; human flow cytometry; rat immunoprecipitation; rat immunocytochemistry; rat western blot; rat	Ozkucur N, Perike S, Sharma P, Funk R. Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness. BMC Cell Biol. 2011;12:4 pubmed publisher
immunoprecipitation; primate western blot; primate	Dynia D, Steinmetz A, Kocinsky H. NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase. Am J Physiol Renal Physiol. 2010;298:F745-53 pubmed publisher
western blot; rat	Kocinsky H, Dynia D, Wang T, Aronson P. NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. Am J Physiol Renal Physiol. 2007;293:F212-8 pubmed
immunocytochemistry; rat immunohistochemistry; rat ELISA; rat western blot; rat immunocytochemistry; primate immunohistochemistry; primate ELISA; primate western blot; primate	Kocinsky H, Girardi A, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, et al. Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites. Am J Physiol Renal Physiol. 2005;289:F249-58 pubmed
	Vitzthum H, Koch M, Eckermann L, Svendsen S, Berg P, H xfc bner C, et al. The AE4 transporter mediates kidney acid-base sensing. Nat Commun. 2023;14:3051 pubmed publisher
	Klinger S. Segment-specific effects of resveratrol on porcine small intestinal dipeptide absorption depend on the mucosal pH and are due to different mechanisms: potential roles of different transport proteins and protein kinases. J Nutr Biochem. 2020;85:108467 pubmed publisher
	Koizumi M, Ueda K, Niimura F, Nishiyama A, Yanagita M, Saito A, et al. Podocyte Injury Augments Intrarenal Angiotensin II Generation and Sodium Retention in a Megalin-Dependent Manner. Hypertension. 2019;74:509-517 pubmed publisher
	Hayasaki T, Ishimoto T, Doke T, Hirayama A, Soga T, Furuhashi K, et al. Fructose increases the activity of sodium hydrogen exchanger in renal proximal tubules that is dependent on ketohexokinase. J Nutr Biochem. 2019;71:54-62 pubmed publisher
	Sunilkumar S, Ford S. Elevated glucose concentration in culture media decreases membrane trafficking of SGLT2 in LLC-PK1 cells via a cAMP/PKA-dependent pathway. Am J Physiol Cell Physiol. 2019;316:C913-C924 pubmed publisher
	Masuda T, Watanabe Y, Fukuda K, Watanabe M, Onishi A, Ohara K, et al. Unmasking a sustained negative effect of SGLT2 inhibition on body fluid volume in the rat. Am J Physiol Renal Physiol. 2018;315:F653-F664 pubmed publisher
	Klinger S, Breves G. Resveratrol Inhibits Porcine Intestinal Glucose and Alanine Transport: Potential Roles of Na?/K?-ATPase Activity, Protein Kinase A, AMP-Activated Protein Kinase and the Association of Selected Nutrient Transport Proteins with Detergent Resistant Memb. Nutrients. 2018;10: pubmed publisher
	Hoesel B, Mussbacher M, Dikorman B, Salzmann M, Assinger A, Hell L, et al. Androgen receptor dampens tissue factor expression via nuclear factor-κB and early growth response protein 1. J Thromb Haemost. 2018;16:749-758 pubmed publisher
	Fenton R, Poulsen S, de la Mora Chavez S, Soleimani M, Dominguez Rieg J, Rieg T. Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis. Kidney Int. 2017;92:397-414 pubmed publisher
	Li Y, Zhang D, Lin D, Cao L. Peroxisome proliferator-activated receptor-Î³ inhibits pancreatic cancer cell invasion and metastasis via regulating MMP-2 expression through PTEN. Mol Med Rep. 2015;12:6255-60 pubmed publisher
	Zhang Z, Jiang T, Li Q, Wang J, Yang D, Li X, et al. Nodal activates smad and extracellular signal-regulated kinases 1/2 pathways promoting renal cell carcinoma proliferation. Mol Med Rep. 2015;12:587-94 pubmed publisher
	Füzesi Levi M, Ben Nissan G, Bianchi E, Zhou H, Deery M, Lilley K, et al. Dynamic regulation of the COP9 signalosome in response to DNA damage. Mol Cell Biol. 2014;34:1066-76 pubmed publisher

product information

master code :

NB110-81529

SKU :

NB110-81529

product name :

NHE3/SLC9A3 [p Ser552] Antibody (14D5) - BSA Free

unit size :

0.1 ml (also 0.025 ml)

description :

The NHE3/SLC9A3 [p Ser552] Antibody (14D5) - BSA Free from Novus is a mouse monoclonal antibody to NHE3/SLC9A3. This antibody reacts with human,mouse,opossum,porcine,primate,rabbit,rat. The NHE3/SLC9A3 [p Ser552] Antibody (14D5) - BSA Free has been validated for the following applications: IF/IHC,ELISA,SDS-Page,Knockout Validated,Knockdown Validated,Flow Cytometry,Immunoprecipitation,Western Blot,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry,Simple Western.

target :

NHE3/SLC9A3

category :

Primary Antibodies

buffer :

Tris-Glycine and 0.15M NaCl

clonality :

Monoclonal

clone :

14D5

concentration :

2 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]

isotype :

IgG2a

purity :

Protein G purified

species :

Human,Mouse,Opossum,Porcine,Primate,Rabbit,Rat

specificity :

This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide

theoretical molecular weight :

85 kDa

gene symbol :

SLC9A3

applications :

IF/IHC,ELISA,SDS-Page,Knockout Validated,Knockdown Validated,Flow Cytometry,Immunoprecipitation,Western Blot,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry,Simple Western

USD :

409 USD

alt names :

isoform 3, MGC126718, NHE3MGC126720, solute carrier family 9 (sodium/hydrogen exchanger), member 3, Solute carrier family 9 member 3

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage