product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rabbit Polyclonal FE65 Antibody
catalog :
NB110-58360F
quantity :
0.1 ml
price :
299.00 USD
clonality :
polyclonal
host :
rabbit
conjugate :
FITC
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB110-58360F
Product Name :
Rabbit Polyclonal FE65 Antibody
List Price :
299.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
Fe65, FE65amyloid beta A4 precursor protein-binding family B member 1, MGC:9072, Protein Fe65, RIR, stat-like protein, adaptor protein FE65a2, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), amyloid beta A4 precursor protein-binding, family B, member 1
Background :
Fe65 (Protein Fe65 or APBB1) is a neuronal adaptor protein that mediates the assembly of multimolecular complexes through a variety of protein-protein interaction domains: the WW domain, which binds to proline-rich sequences, and two C-terminal phosphotyrosine-binding domains. FE65 is primarily found in CNS and is highly expressed in neurons of hippocampus, cerebellum, thalamus, and brain stem nuclei. Fe65 is localized within cytoplasm or nucleus, although its moderate amounts are tethered to membranes via binding with Alzheimer's disease amyloid precursor protein (APP). FE65 forms stable, transcriptionally active complex with AICD (APP intracellular domain) that follows nuclear translocation of AICD and apoptosis through Tip60. FE65 is functionally linked to cellular motility, morphology and actin dynamics through binding of its WW domain to actin-binding protein Mena. It plays a key role in response to DNA damage by translocating to nucleus and inducing apoptosis through recognizing/binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at DSBs, recruiting other pro-apoptosis factors such as MAPK8/JNK1. It is required for histone H4 acetylation DSBs and its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. It functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor that inhibits the expression of CASP4.
Buffer :
50mM sodium borate
Clonality :
Polyclonal
Conjugate :
FITC
Application Summary :
Western Blot, Immunoprecipitation
Host :
Rabbit
Immunogen :
Recombinant rat FE65, expressed in bacteria. [Swiss-Prot# P46933]
Localization :
Cytoplasmic
Preservative :
No Preservative
Purity :
Immunogen affinity purified
CrossReactivity :
Rat, human, and mouse.
Storage :
Store at 4C in the dark.
TMW :
100 kDa
Uses :
This FE65 antibody is useful in Western blot and Immunoprecipitation. In Western blot a band is seen at ~100 kDa versus the predicted molecular weight of 77 kDa, which corresponds to earlier findings in literature (PMID: 9685356).
Research Area :
Alzheimers Research, Cell Cycle and Replication, Neurodegeneration, Neuroscience, Phospho Specific, Signal Transduction
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.