product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rabbit Polyclonal Podocalyxin Antibody
catalog :
NB110-41510F
quantity :
0.1 ml
price :
309.00 USD
clonality :
polyclonal
host :
rabbit
conjugate :
FITC
reactivity :
human
application :
western blot
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB110-41510F
Product Name :
Rabbit Polyclonal Podocalyxin Antibody
List Price :
309.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
Gp200MGC138240, PCLP1, PCLP-1, PCLPGCTM-2 antigen, PCpodocalyxin, podocalyxin-like, Podocalyxin-like protein 1
Background :
Podocalyxin (PODXL) is an extensively N/O-glycosylated and sialylated type I transmembrane protein that contains C-terminal PDZ-binding motif DTHL capable of facilitating interactions with NHERF1/NHERF2 adaptor proteins which complexes with proteins implicated in ion transport, protein trafficking and signal transduction. PODXL function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in podocytes by charge repulsion. PODXL also acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. It induces apical actin-dependent microvilli formation and is implicated preapical plasma membrane subdomain formation to set up inital epithelial polarization and apical lumen formation during renal tubulogenesis. PODXL is highly expressed in renal glomeruli, embryonic germ layers, hematopoietic progenitors, megakaryocytes and platelets, vascular endothelia, mesothelial cells lining organs, and neurons. In several malignancies such as leukemia and cancer of breast, testicular, prostate etc, PODXL interactions with actin-binding protein EZR enhances cancer aggressiveness by inducing cell migration and invasion through via modulation of MAPK/PI3K signaling.
Buffer :
50mM sodium borate
Clonality :
Polyclonal
Conjugate :
FITC
Application Summary :
Western Blot
Host :
Rabbit
Immunogen :
A synthetic peptide within an internal region (residues 350-450) of the human Podocalyxin protein. [Swiss-Prot# O00592]
Localization :
Membrane
Marker :
Podocytes /Apical Marker, Endothelial Cell Marker
Preservative :
No Preservative
Purity :
Immunogen affinity purified
CrossReactivity :
Human and mouse. Immunogen sequence has 80% homology to canine and 76% homology to rat.
Storage :
Store at 4C in the dark.
Swissprot Human :
O00592
Uses :
This Podocalyxin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. In WB a band is observed at ~52 kDa. There may be a non-specific band observed at ~27 kDa depending on the lysates used. In ICC/IF membrane staining was observed.
Research Area :
Cancer, Cell Biology, Cellular Markers, Embryonic Stem Cell Markers, Hematopoietic Stem Cell Markers, Stem Cell Markers
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.