product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rabbit Polyclonal Perilipin-2/ADFP Antibody
catalog :
NB110-40877H
quantity :
0.1 ml
price :
319 USD
clonality :
polyclonal
host :
rabbit
conjugate :
HRP
reactivity :
human, rat, mouse
application :
western blot
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB110-40877H
Product Name :
Rabbit Polyclonal Perilipin-2/ADFP Antibody
List Price :
319 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
ADFP, Adipophilin, Adipose differentiation-related proteinadipophilin, ADRP, MGC10598, perilipin 2, perilipin-2
Background :
Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase. ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.
Buffer :
PBS
Clonality :
Polyclonal
Concentration :
1.0 mg/ml
Conjugate :
HRP
Application Summary :
Western Blot
Host :
Rabbit
Immunogen :
A synthetic peptide made to a C-terminal region of mouse ADFP (within residues 350-425) [Swiss-Prot# P43883]
Localization :
Membrane
Preservative :
No Preservative
Purity :
Immunogen affinity purified
CrossReactivity :
Human and mouse. Immunogen has 88% sequence identity with rat ADFP. Customer feedback has been negative on trout liver.
Storage :
Store at 4C in the dark.
Swissprot Human :
Q99541
TMW :
51 kDa
Uses :
This ADFP antibody can be used for Western blot and Immunofluorescence/Immunocytochemistry. In Western blot a band is observed at ~51 kDa. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, staining was observed on the membrane of mouse liver cells Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Research Area :
Cancer, Lipid and Metabolism, Lipid Droplets, Signal Transduction
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.