product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free
catalog :
NB110-3638
quantity :
0.1 ml (also 0.025 ml)
price :
419 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8B6
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 40
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human
Forbes K, Desforges M, Garside R, Aplin J, Westwood M. Methods for siRNA-mediated reduction of mRNA and protein expression in human placental explants, isolated primary cells and cell lines. Placenta. 2009;30:124-9 pubmed publisher
  • immunocytochemistry; human
Johnstone E, Chan G, Sibley C, Davidge S, Lowen B, Guilbert L. Sphingosine-1-phosphate inhibition of placental trophoblast differentiation through a G(i)-coupled receptor response. J Lipid Res. 2005;46:1833-9 pubmed
  • immunohistochemistry - frozen section; human
  • immunocytochemistry; human
Leitner K, Szlauer R, Ellinger I, Ellinger A, Zimmer K, Fuchs R. Placental alkaline phosphatase expression at the apical and basal plasma membrane in term villous trophoblasts. J Histochem Cytochem. 2001;49:1155-64 pubmed
  • flow cytometry; human
Feehan C, Darlak K, Kahn J, Walcheck B, Spatola A, Kishimoto T. Shedding of the lymphocyte L-selectin adhesion molecule is inhibited by a hydroxamic acid-based protease inhibitor. Identification with an L-selectin-alkaline phosphatase reporter. J Biol Chem. 1996;271:7019-24 pubmed
  • western blot; human
Roberts S, Ripellino J, Ingalls K, Robakis N, Felsenstein K. Non-amyloidogenic cleavage of the beta-amyloid precursor protein by an integral membrane metalloendopeptidase. J Biol Chem. 1994;269:3111-6 pubmed
  • immunohistochemistry - frozen section; human
Kesson A, Fear W, Williams L, Chang J, King N, Cunningham A. HIV infection of placental macrophages: their potential role in vertical transmission. J Leukoc Biol. 1994;56:241-6 pubmed
Qiu M, Tulufu N, Tang G, Ye W, Qi J, Deng L, et al. Black Phosphorus Accelerates Bone Regeneration Based on Immunoregulation. Adv Sci (Weinh). 2024;11:e2304824 pubmed publisher
Qiu M, Li C, Cai Z, Li C, Yang K, Tulufu N, et al. 3D Biomimetic Calcified Cartilaginous Callus that Induces Type H Vessels Formation and Osteoclastogenesis. Adv Sci (Weinh). 2023;10:e2207089 pubmed publisher
Chan Y, Ho K, Lee Y, Chou M, Lew W, Huang H, et al. Melatonin enhances osteogenic differentiation of dental pulp mesenchymal stem cells by regulating MAPK pathways and promotes the efficiency of bone regeneration in calvarial bone defects. Stem Cell Res Ther. 2022;13:73 pubmed publisher
Ontawong A, Duangjai A, Srimaroeng C. Coffea arabica bean extract inhibits glucose transport and disaccharidase activity in Caco-2 cells. Biomed Rep. 2021;15:73 pubmed publisher
Redhu A, Bhat J. Mitochondrial glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase abrogate p53 induced apoptosis in a yeast model: Possible implications for apoptosis resistance in cancer cells. Biochim Biophys Acta Gen Subj. 2020;1864:129504 pubmed publisher
Zhao S, Suda Y, Nakanishi H, Wang N, Yoko o T, Gao X, et al. Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network. Biochim Biophys Acta Gen Subj. 2019;: pubmed publisher
Audesse A, Dhakal S, Hassell L, Gardell Z, Nemtsova Y, Webb A. FOXO3 directly regulates an autophagy network to functionally regulate proteostasis in adult neural stem cells. PLoS Genet. 2019;15:e1008097 pubmed publisher
Chung Y, Luo A, Park K, Loonat A, Lakhal Littleton S, Robbins P, et al. Iron-deficiency anemia reduces cardiac contraction by downregulating RyR2 channels and suppressing SERCA pump activity. JCI Insight. 2019;4: pubmed publisher
Taylor S, Giuffre E, Moseley P, Hitchcock P. The MicroRNA, miR-18a, Regulates NeuroD and Photoreceptor Differentiation in the Retina of Zebrafish. Dev Neurobiol. 2019;79:202-219 pubmed publisher
Ontawong A, Duangjai A, Muanprasat C, Pasachan T, Pongchaidecha A, Amornlerdpison D, et al. Lipid-lowering effects of Coffea arabica pulp aqueous extract in Caco-2 cells and hypercholesterolemic rats. Phytomedicine. 2019;52:187-197 pubmed publisher
Goulao M, Ghosh B, Urban M, Sahu M, Mercogliano C, Charsar B, et al. Astrocyte progenitor transplantation promotes regeneration of bulbospinal respiratory axons, recovery of diaphragm function, and a reduced macrophage response following cervical spinal cord injury. Glia. 2019;67:452-466 pubmed publisher
Tsai F, Ho M, Lai C, Chou C, Li J, Cheng C, et al. Evaluation of Oral Antiretroviral Drugs in Mice With Metabolic and Neurologic Complications. Front Pharmacol. 2018;9:1004 pubmed publisher
Yang Q, Wang P, Du X, Wang W, Zhang T, Chen Y. Direct repression of IGF2 is implicated in the anti-angiogenic function of microRNA-210 in human retinal endothelial cells. Angiogenesis. 2018;21:313-323 pubmed publisher
Kim J, Singh A, Del Poeta M, Brown D, London E. The effect of sterol structure upon clathrin-mediated and clathrin-independent endocytosis. J Cell Sci. 2017;130:2682-2695 pubmed publisher
Luévano Martínez L, Forni M, Peloggia J, Watanabe I, Kowaltowski A. Calorie restriction promotes cardiolipin biosynthesis and distribution between mitochondrial membranes. Mech Ageing Dev. 2017;162:9-17 pubmed publisher
Merianda T, Jin Y, Kalinski A, Sahoo P, Fischer I, Twiss J. Neural Progenitor Cells Promote Axonal Growth and Alter Axonal mRNA Localization in Adult Neurons. Eneuro. 2017;4: pubmed publisher
Mana G, Clapero F, Panieri E, Panero V, Böttcher R, Tseng H, et al. PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis. Nat Commun. 2016;7:13546 pubmed publisher
Wang P, Du X, Xiong M, Cui J, Yang Q, Wang W, et al. Ginsenoside Rd attenuates breast cancer metastasis implicating derepressing microRNA-18a-regulated Smad2 expression. Sci Rep. 2016;6:33709 pubmed publisher
Yang Q, Wang P, Cui J, Wang W, Chen Y, Zhang T. Panax notoginseng saponins attenuate lung cancer growth in part through modulating the level of Met/miR-222 axis. J Ethnopharmacol. 2016;193:255-265 pubmed publisher
Yang Q, Cui J, Wang P, Du X, Wang W, Zhang T, et al. Changes in interconnected pathways implicating microRNAs are associated with the activity of apocynin in attenuating myocardial fibrogenesis. Eur J Pharmacol. 2016;784:22-32 pubmed publisher
Lin Y, Ho T, Yeh Y, Cheng C, Shiao Y, Wang C, et al. Chinese Herbal Medicine Treatment Improves the Overall Survival Rate of Individuals with Hypertension among Type 2 Diabetes Patients and Modulates In Vitro Smooth Muscle Cell Contractility. PLoS ONE. 2015;10:e0145109 pubmed publisher
Pei C, Qin S, Wang M, Zhang S. Regulatory mechanism of human vascular smooth muscle cell phenotypic transformation induced by NELIN. Mol Med Rep. 2015;12:7310-6 pubmed publisher
Tabolacci C, Cordella M, Turcano L, Rossi S, Lentini A, Mariotti S, et al. Aloe-emodin exerts a potent anticancer and immunomodulatory activity on BRAF-mutated human melanoma cells. Eur J Pharmacol. 2015;762:283-92 pubmed publisher
Beckmann E, Köhler A, Meister C, Christmann M, Draht O, Rakebrandt N, et al. Integration of the catalytic subunit activates deneddylase activity in vivo as final step in fungal COP9 signalosome assembly. Mol Microbiol. 2015;97:110-24 pubmed publisher
Lehneck R, Elleuche S, Pöggeler S. The filamentous ascomycete Sordaria macrospora can survive in ambient air without carbonic anhydrases. Mol Microbiol. 2014;92:931-44 pubmed publisher
Cary G, Yoon S, Torres C, Wang K, Hays M, Ludlow C, et al. Identification and characterization of a drug-sensitive strain enables puromycin-based translational assays in Saccharomyces cerevisiae. Yeast. 2014;31:167-78 pubmed publisher
Christmann M, Schmaler T, Gordon C, Huang X, Bayram Ö, Schinke J, et al. Control of multicellular development by the physically interacting deneddylases DEN1/DenA and COP9 signalosome. PLoS Genet. 2013;9:e1003275 pubmed publisher
Pradines E, Hernandez Rapp J, Villa Diaz A, Dakowski C, Ardila Osorio H, Haik S, et al. Pathogenic prions deviate PrP(C) signaling in neuronal cells and impair A-beta clearance. Cell Death Dis. 2013;4:e456 pubmed publisher
Voigt O, Pöggeler S. Autophagy genes Smatg8 and Smatg4 are required for fruiting-body development, vegetative growth and ascospore germination in the filamentous ascomycete Sordaria macrospora. Autophagy. 2013;9:33-49 pubmed publisher
Bernard D, Cheng Y, Zhao Y, Balk J. An allelic mutant series of ATM3 reveals its key role in the biogenesis of cytosolic iron-sulfur proteins in Arabidopsis. Plant Physiol. 2009;151:590-602 pubmed publisher
Bych K, Netz D, Vigani G, Bill E, Lill R, Pierik A, et al. The essential cytosolic iron-sulfur protein Nbp35 acts without Cfd1 partner in the green lineage. J Biol Chem. 2008;283:35797-804 pubmed publisher
Zheng L, Anderson R, Agbaga M, Rucker E, Le Y. Loss of BCL-XL in rod photoreceptors: Increased susceptibility to bright light stress. Invest Ophthalmol Vis Sci. 2006;47:5583-9 pubmed
Kandasamy M, McKinney E, Meagher R. The late pollen-specific actins in angiosperms. Plant J. 1999;18:681-91 pubmed
Kiem H, Andrews R, Morris J, Peterson L, Heyward S, Allen J, et al. Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor. Blood. 1998;92:1878-86 pubmed
product information
master code :
NB110-3638
SKU :
NB110-3638
product name :
Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free
unit size :
0.1 ml (also 0.025 ml)
description :
The Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free from Novus is a mouse monoclonal antibody to Alkaline Phosphatase/ALPP. This antibody reacts with human,mouse,rat. The Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free has been validated for the following applications: IF/IHC,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,ELISA,Immunohistochemistry,Radioimmunodiffusion.
target :
Alkaline Phosphatase/ALPP
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
8B6
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
isotype :
IgG2a Kappa
purity :
Protein A purified
species :
Human,Mouse,Rat
specificity :
Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes
theoretical molecular weight :
70 kDa
gene symbol :
ALPP
accessionNumbers :
P05187
applications :
IF/IHC,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,ELISA,Immunohistochemistry,Radioimmunodiffusion
USD :
419 USD
alt names :
Alkaline phosphatase Regan isozyme, alkaline phosphatase, placental, alkaline phosphatase, placental (Regan isozyme), alkaline phosphatase, placental type, alkaline phosphomonoesterase, ALP, EC 3.1.3.1, FLJ61142, glycerophosphatase, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1, Regan isozyme
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.