product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Mouse Monoclonal BRCA1 Antibody
catalog :
NB100-598R
quantity :
0.1 ml
price :
319.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
DyLight 550
reactivity :
human
application :
western blot, immunocytochemistry, immunoprecipitation
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB100-598R
Product Name :
Mouse Monoclonal BRCA1 Antibody
List Price :
319.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1, BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein
Background :
BRCA1 (breast and ovarian cancer susceptibility protein 1) is a RING finger protein containing a BRCT domain. BRCA1 exists as a heterodimer with 22 possible isoforms. The full length protein has a reported molecular weight of 208 kD. BRCA1 localizes to the mitotic spindle microtubules, centriole walls, pericentriolar fibers at centrosomes. Unphosphorylated BRCA1 localizes on chromosomes from metaphase through telophase; phosphorylated BRCA1 resides in inner chromosomal structure, centrosome, cleavage furrow during prophase through telophase, and relocalizes to the perinuclear region when cells are subjected to IR or UV radiation in S phase. BRCA1 acts as a tumor suppressor and can function as a secreted growth inhibitory protein, participate in transcription coupled repair of oxidative DNA damage, X-chromosome inactivation, and can function as a E3 ubiquitin ligase. BRCA1 can be transcriptionally downregulated by Ets-2, Brg-1, and Hmga-1. BRCA1 can be modified by glycosylation, ubiquitination and phosphorylation by CDK4, ATM/ATR, cdk2, and hChk2. The BRCA1 protein has been reported to interact with RNA polymerase II holoenzyme and BARD1. BRCA1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA1 protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA1 protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA1 function confers an increased susceptibility for both breast and ovarian cancer.
Buffer :
50mM sodium borate
Clonality :
Monoclonal
Clone :
RAY
Conjugate :
DyLight 550
Application Summary :
Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation
Epitope :
The epitope recognized is found between amino acids 1314-1600.
Host :
Mouse
Immunogen :
Human BRCA1 corresponding to residues 1314-1864 [UniProt# P38398]
Isotype :
IgG2a
Localization :
nuclear
Preservative :
No Preservative
Purity :
Protein G purified
CrossReactivity :
Human
Storage :
Store at 4C in the dark.
Swissprot Human :
P38398
TMW :
230 kDa
Uses :
This BRCA1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot a band was observed ~220-240 kDa.
Research Area :
Apoptosis, Breast Cancer, Cancer, Cell Biology, Chromatin Research, DNA Double Strand Break Repair, DNA Repair, Homologous Recombination, Tumor Suppressors, Ubiquitin Proteasome Pathway, Zinc Finger
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.