TLR4 Antibody (HTA125) - Azide and BSA Free | Novus Biologicals NB100-56727 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

TLR4 Antibody (HTA125) - Azide and BSA Free

catalog :

NB100-56727

quantity :

0.1 mg (also 0.025 mg)

price :

479 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HTA125

reactivity :

human, mouse, dogs

application :

western blot, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry

more info or order :

Novus Biologicals product webpage

citations: 26

Reference
Ji X, Zhang X, Sun L, Hou X, Song H, Han L, et al. The Heparin-Binding Hemagglutinin of Nocardia cyriacigeorgica GUH-2 Stimulates Inflammatory Cytokine Secretion Through Activation of Nuclear Factor κB and Mitogen-Activated Protein Kinase Pathways via TLR4. Front Cell Infect Microbiol. 2020;10:3 pubmed publisher
The E, Yao Q, Zhang P, Zhai Y, Ao L, Fullerton D, et al. Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells. Cells. 2020;9: pubmed publisher
Ji X, Zhang X, Li H, Sun L, Hou X, Song H, et al. Nfa34810 Facilitates Nocardia farcinica Invasion of Host Cells and Stimulates Tumor Necrosis Factor Alpha Secretion through Activation of the NF-κB and Mitogen-Activated Protein Kinase Pathways via Toll-Like Receptor 4. Infect Immun. 2020;88: pubmed publisher
Li F, Song R, Ao L, Reece T, Cleveland J, Dong N, et al. ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells. Arterioscler Thromb Vasc Biol. 2017;37:1339-1351 pubmed publisher
KÃ¤bisch R, MejÃas Luque R, Gerhard M, Prinz C. Involvement of Toll-like receptors on Helicobacter pylori-induced immunity. PLoS ONE. 2014;9:e104804 pubmed publisher
Rojas Bernabé A, Garcia Hernández O, Maldonado Bernal C, Delegado Dominguez J, Ortega E, Gutiérrez Kobeh L, et al. Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4. Parasitology. 2014;141:788-800 pubmed publisher
Harman A, Bye C, Nasr N, Sandgren K, Kim M, Mercier S, et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013;190:66-79 pubmed publisher
Matsunaga N, Tsuchimori N, Matsumoto T, Ii M. TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules. Mol Pharmacol. 2011;79:34-41 pubmed publisher
Pillay J, Ramakers B, Kamp V, Loi A, Lam S, Hietbrink F, et al. Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia. J Leukoc Biol. 2010;88:211-20 pubmed publisher
Wu C, Chi P, Hsieh H, Luo S, Yang C. TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2. J Cell Physiol. 2010;223:480-91 pubmed publisher
Pietschmann K, Beetz S, Welte S, Martens I, Gruen J, Oberg H, et al. Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes. Scand J Immunol. 2009;70:245-55 pubmed publisher
O Hara S, Small A, Gajdos G, Badley A, Chen X, Larusso N. HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum. J Infect Dis. 2009;199:1195-204 pubmed publisher
Hammadi A, Billard C, Faussat A, Kolb J. Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation. Nitric Oxide. 2008;19:138-45 pubmed publisher
Burgener I, Jungi T. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008;124:184-91 pubmed publisher
Prabha C, Rajashree P, Sulochana D. TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid. Immunol Lett. 2008;117:26-34 pubmed publisher
Pegu A, Qin S, Fallert Junecko B, Nisato R, Pepper M, Reinhart T. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008;180:3399-405 pubmed
Murciano C, Villamon E, Yáñez A, Murciano J, Mir A, O Connor J, et al. In vitro response to Candida albicans in cultures of whole human blood from young and aged donors. FEMS Immunol Med Microbiol. 2007;51:327-35 pubmed
Wong C, Cheung P, Ip W, Lam C. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007;37:85-96 pubmed
Basu S, Pathak S, Banerjee A, Pathak S, Bhattacharyya A, Yang Z, et al. Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha. J Biol Chem. 2007;282:1039-50 pubmed
Shahrara S, Park C, Temkin V, Jarvis J, Volin M, Pope R. RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes. J Immunol. 2006;177:5077-87 pubmed
Scheel O, Papavlassopoulos M, Blunck R, Gebert A, Hartung T, Zahringer U, et al. Cell activation by ligands of the toll-like receptor and interleukin-1 receptor family depends on the function of the large-conductance potassium channel MaxiK in human macrophages. Infect Immun. 2006;74:4354-6 pubmed
Konno K, Wakabayashi Y, Akashi Takamura S, Ishii T, Kobayashi M, Takahashi K, et al. A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression. Biochem Biophys Res Commun. 2006;339:1076-82 pubmed
Cognasse F, Hamzeh H, Chavarin P, Acquart S, Genin C, Garraud O. Evidence of Toll-like receptor molecules on human platelets. Immunol Cell Biol. 2005;83:196-8 pubmed
Basak C, Pathak S, Bhattacharyya A, Mandal D, Pathak S, Kundu M. NF-kappaB- and C/EBPbeta-driven interleukin-1beta gene expression and PAK1-mediated caspase-1 activation play essential roles in interleukin-1beta release from Helicobacter pylori lipopolysaccharide-stimulated macrophages. J Biol Chem. 2005;280:4279-88 pubmed
Mempel M, Voelcker V, Köllisch G, Plank C, Rad R, Gerhard M, et al. Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent. J Invest Dermatol. 2003;121:1389-96 pubmed
Lotz S, Aga E, Wilde I, van Zandbergen G, Hartung T, Solbach W, et al. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 2004;75:467-77 pubmed

image

image 1 :

Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Staining of human peripheral blood monocytes with Mouse anti Human CD284.

product information

brand :

Novus Biologicals, a Bio-Techne brand

catalog number base :

NB100-56727

SKU :

NB100-56727

product name :

TLR4 Antibody (HTA125) - Azide and BSA Free

description :

The TLR4 Antibody (HTA125) - Azide and BSA Free from Novus Biologicals is a mouse monoclonal antibody to TLR4. This antibody reacts with canine,human,mouse. The TLR4 Antibody (HTA125) - Azide and BSA Free has been validated for the following applications: Immunoprecipitation,Immunoblotting,Flow (Intracellular),CyTOF-ready,Flow Cytometry,Western Blot,In vitro assay,Flow (Cell Surface),Block/Neutralize,Immunocytochemistry/ Immunofluorescence.

target :

TLR4

unit size :

0.1 mg (also 0.025 mg)

category :

Primary Antibodies

buffer :

Filter (0.2 micron) sterilized PBS

clonality :

Monoclonal

clone :

HTA125

concentration :

0.5 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

This TLR4 Antibody (HTA125) was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.

isotype :

IgG2a

purity :

Protein G purified

species :

Canine,Human,Mouse

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

theoretical molecular weight :

95.7 kDa

gene symbol :

TLR4

applications :

Immunoprecipitation,Immunoblotting,Flow (Intracellular),CyTOF-ready,Flow Cytometry,Western Blot,In vitro assay,Flow (Cell Surface),Block/Neutralize,Immunocytochemistry/ Immunofluorescence

extended description :

Clone HTA125 was used by HLDA to establish CD designation.

USD :

449

USD 2025 :

479 USD

product details :

Clone HTA125 was used by HLDA to establish CD designation.

alt names :

ARMD10, CD_antigen: CD284, CD284, CD284 antigen, EC 3.2.2.6, EC:3.2.2.6, homolog of Drosophila toll, hToll, TLR4, TLR-4, TOLL, toll like receptor 4 protein, toll-like receptor 4

more info or order :

Novus Biologicals product webpage