TLR4 Antibody (HTA125) - BSA Free | Novus Biologicals NB100-56723 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

TLR4 Antibody (HTA125) - BSA Free

catalog :

NB100-56723

quantity :

0.1 mg (also 0.025 mg)

price :

379 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HTA125

reactivity :

human, mouse, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Novus Biologicals product webpage

citations: 54

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 4b	Guo D, Yamamoto M, Hernandez C, Khodadadi H, Baban B, Stranahan A. Beige adipocytes mediate the neuroprotective and anti-inflammatory effects of subcutaneous fat in obese mice. Nat Commun. 2021;12:4623 pubmed publisher
immunohistochemistry - paraffin section; human	Youn J, Shim H, Lee J, Ji A, Oh J, Hong N, et al. Detailed pathologic evaluation on endomyocardial biopsy provides long-term prognostic information in patients with acute myocarditis. Cardiovasc Pathol. 2014;23:139-44 pubmed publisher
immunocytochemistry; human	Chen X, Splinter P, O Hara S, Larusso N. A cellular micro-RNA, let-7i, regulates Toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection. J Biol Chem. 2007;282:28929-38 pubmed
flow cytometry; human	Basu S, Pathak S, Banerjee A, Pathak S, Bhattacharyya A, Yang Z, et al. Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha. J Biol Chem. 2007;282:1039-50 pubmed
immunocytochemistry; human	Schneeman T, Bruno M, Schjerven H, Johansen F, Chady L, Kaetzel C. Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: linking innate and adaptive immune responses. J Immunol. 2005;175:376-84 pubmed
immunocytochemistry; human	Srivastava M, Srivastava B. Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line. Leuk Res. 2005;29:813-20 pubmed
immunocytochemistry; human	Mempel M, Voelcker V, Köllisch G, Plank C, Rad R, Gerhard M, et al. Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent. J Invest Dermatol. 2003;121:1389-96 pubmed
flow cytometry; human	Lotz S, Aga E, Wilde I, van Zandbergen G, Hartung T, Solbach W, et al. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 2004;75:467-77 pubmed
	Ji X, Zhang X, Sun L, Hou X, Song H, Han L, et al. The Heparin-Binding Hemagglutinin of Nocardia cyriacigeorgica GUH-2 Stimulates Inflammatory Cytokine Secretion Through Activation of Nuclear Factor κB and Mitogen-Activated Protein Kinase Pathways via TLR4. Front Cell Infect Microbiol. 2020;10:3 pubmed publisher
	The E, Yao Q, Zhang P, Zhai Y, Ao L, Fullerton D, et al. Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells. Cells. 2020;9: pubmed publisher
	Ji X, Zhang X, Li H, Sun L, Hou X, Song H, et al. Nfa34810 Facilitates Nocardia farcinica Invasion of Host Cells and Stimulates Tumor Necrosis Factor Alpha Secretion through Activation of the NF-κB and Mitogen-Activated Protein Kinase Pathways via Toll-Like Receptor 4. Infect Immun. 2020;88: pubmed publisher
	Akhter N, Madhoun A, Arefanian H, Wilson A, Kochumon S, Thomas R, et al. Oxidative Stress Induces Expression of the Toll-Like Receptors (TLRs) 2 and 4 in the Human Peripheral Blood Mononuclear Cells: Implications for Metabolic Inflammation. Cell Physiol Biochem. 2019;53:1-18 pubmed publisher
	Polari L, Carneiro P, Macedo M, Machado P, Scott P, Carvalho E, et al. Leishmania braziliensis Infection Enhances Toll-Like Receptors 2 and 4 Expression and Triggers TNF-α and IL-10 Production in Human Cutaneous Leishmaniasis. Front Cell Infect Microbiol. 2019;9:120 pubmed publisher
	Coelho da Silva F, Covre L, Stringari L, Palaci M, Dietze R, Gomes D, et al. Toll-like receptors blocking restores in vitro microbicidal activity in latent tuberculosis-infected subjects. Int J Tuberc Lung Dis. 2019;23:212-218 pubmed publisher
	Ahmad R, Al Roub A, Kochumon S, Akther N, Thomas R, Kumari M, et al. The Synergy between Palmitate and TNF-α for CCL2 Production Is Dependent on the TRIF/IRF3 Pathway: Implications for Metabolic Inflammation. J Immunol. 2018;200:3599-3611 pubmed publisher
	Li F, Song R, Ao L, Reece T, Cleveland J, Dong N, et al. ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells. Arterioscler Thromb Vasc Biol. 2017;37:1339-1351 pubmed publisher
	Heftrig D, Sturm R, Oppermann E, Kontradowitz K, Jurida K, Schimunek L, et al. Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients. Mediators Inflamm. 2017;2017:2608349 pubmed publisher
	Larsson O, Tengroth L, Xu Y, Uddman R, Kumlien Georén S, Cardell L. Substance P represents a novel first-line defense mechanism in the nose. J Allergy Clin Immunol. 2018;141:128-136.e3 pubmed publisher
	Zeng Q, Song R, Fullerton D, Ao L, Zhai Y, Li S, et al. Interleukin-37 suppresses the osteogenic responses of human aortic valve interstitial cells in vitro and alleviates valve lesions in mice. Proc Natl Acad Sci U S A. 2017;114:1631-1636 pubmed publisher
	Imai H, Fujita T, Kajiya M, Ouhara K, Yoshimoto T, Matsuda S, et al. Mobilization of TLR4 Into Lipid Rafts by Aggregatibacter Actinomycetemcomitans in Gingival Epithelial Cells. Cell Physiol Biochem. 2016;39:1777-1786 pubmed
	Schmid W, Novacek G, Vogelsang H, Papay P, Primas C, Eser A, et al. Platelets Toll-like receptor-4 in Crohns disease. Eur J Clin Invest. 2017;47:109-116 pubmed publisher
	Moreira M, Costa Pereira C, Alves M, Marteleto B, Ribeiro V, Peruhype MagalhÃ£es V, et al. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Vet Parasitol. 2016;220:33-45 pubmed publisher
	Kopp F, Kupsch S, Schromm A. Lipopolysaccharide-binding protein is bound and internalized by host cells and colocalizes with LPS in the cytoplasm: Implications for a role of LBP in intracellular LPS-signaling. Biochim Biophys Acta. 2016;1863:660-72 pubmed publisher
	PÃ©rez Figueroa E, Torres J, SÃ¡nchez Zauco N, Contreras Ramos A, Alvarez Arellano L, Maldonado Bernal C. Activation of NLRP3 inflammasome in human neutrophils by Helicobacter pylori infection. Innate Immun. 2016;22:103-12 pubmed publisher
	Jamin A, Dehoux L, Dossier C, Fila M, Heming N, Monteiro R, et al. Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome. Clin Exp Immunol. 2015;182:332-45 pubmed publisher
	Komine Aizawa S, Hirohata N, Aizawa S, Abiko Y, Hayakawa S. Porphyromonas gingivalis lipopolysaccharide inhibits trophoblast invasion in the presence of nicotine. Placenta. 2015;36:27-33 pubmed publisher
	Song R, Fullerton D, Ao L, Zheng D, Zhao K, Meng X. BMP-2 and TGF-Î²1 mediate biglycan-induced pro-osteogenic reprogramming in aortic valve interstitial cells. J Mol Med (Berl). 2015;93:403-12 pubmed publisher
	KÃ¤bisch R, MejÃas Luque R, Gerhard M, Prinz C. Involvement of Toll-like receptors on Helicobacter pylori-induced immunity. PLoS ONE. 2014;9:e104804 pubmed publisher
	Feng X, Feng G, Xing J, Shen B, Tan W, Huang D, et al. Repeated lipopolysaccharide stimulation promotes cellular senescence in human dental pulp stem cells (DPSCs). Cell Tissue Res. 2014;356:369-80 pubmed publisher
	Rojas Bernabé A, Garcia Hernández O, Maldonado Bernal C, Delegado Dominguez J, Ortega E, Gutiérrez Kobeh L, et al. Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4. Parasitology. 2014;141:788-800 pubmed publisher
	Yuan X, Zhou Y, Wang W, Li J, Xie G, Zhao Y, et al. Activation of TLR4 signaling promotes gastric cancer progression by inducing mitochondrial ROS production. Cell Death Dis. 2013;4:e794 pubmed publisher
	Harman A, Bye C, Nasr N, Sandgren K, Kim M, Mercier S, et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013;190:66-79 pubmed publisher
	Matsunaga N, Tsuchimori N, Matsumoto T, Ii M. TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules. Mol Pharmacol. 2011;79:34-41 pubmed publisher
	Pillay J, Ramakers B, Kamp V, Loi A, Lam S, Hietbrink F, et al. Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia. J Leukoc Biol. 2010;88:211-20 pubmed publisher
	Wu C, Chi P, Hsieh H, Luo S, Yang C. TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2. J Cell Physiol. 2010;223:480-91 pubmed publisher
	Pietschmann K, Beetz S, Welte S, Martens I, Gruen J, Oberg H, et al. Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes. Scand J Immunol. 2009;70:245-55 pubmed publisher
	O Hara S, Small A, Gajdos G, Badley A, Chen X, Larusso N. HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum. J Infect Dis. 2009;199:1195-204 pubmed publisher
	Yang X, Fullerton D, Su X, Ao L, Cleveland J, Meng X. Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2. J Am Coll Cardiol. 2009;53:491-500 pubmed publisher
	Hammadi A, Billard C, Faussat A, Kolb J. Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation. Nitric Oxide. 2008;19:138-45 pubmed publisher
	Burgener I, Jungi T. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008;124:184-91 pubmed publisher
	Prabha C, Rajashree P, Sulochana D. TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid. Immunol Lett. 2008;117:26-34 pubmed publisher
	Pegu A, Qin S, Fallert Junecko B, Nisato R, Pepper M, Reinhart T. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008;180:3399-405 pubmed
	Yang F, Hua K, Yang Y, Zou W, Chen Y, Liang S, et al. TLR-independent induction of human monocyte IL-1 by phosphoglycolipids from thermophilic bacteria. Glycoconj J. 2008;25:427-39 pubmed
	Kundu S, Lee C, Billips B, Habermacher G, Zhang Q, Liu V, et al. The toll-like receptor pathway: a novel mechanism of infection-induced carcinogenesis of prostate epithelial cells. Prostate. 2008;68:223-9 pubmed
	Murciano C, Villamon E, Yáñez A, Murciano J, Mir A, O Connor J, et al. In vitro response to Candida albicans in cultures of whole human blood from young and aged donors. FEMS Immunol Med Microbiol. 2007;51:327-35 pubmed
	Wong C, Cheung P, Ip W, Lam C. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007;37:85-96 pubmed
	Shahrara S, Park C, Temkin V, Jarvis J, Volin M, Pope R. RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes. J Immunol. 2006;177:5077-87 pubmed
	Zanoni G, Navone R, Lunardi C, Tridente G, Bason C, Sivori S, et al. In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of monocytes. PLoS Med. 2006;3:e358 pubmed
	Xu S, Koldovsky U, Xu M, Wang D, Fitzpatrick E, Son G, et al. High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production. Surgery. 2006;140:170-8 pubmed
	Scheel O, Papavlassopoulos M, Blunck R, Gebert A, Hartung T, Zahringer U, et al. Cell activation by ligands of the toll-like receptor and interleukin-1 receptor family depends on the function of the large-conductance potassium channel MaxiK in human macrophages. Infect Immun. 2006;74:4354-6 pubmed
	Rallabhandi P, Bell J, Boukhvalova M, Medvedev A, Lorenz E, Arditi M, et al. Analysis of TLR4 polymorphic variants: new insights into TLR4/MD-2/CD14 stoichiometry, structure, and signaling. J Immunol. 2006;177:322-32 pubmed
	Konno K, Wakabayashi Y, Akashi Takamura S, Ishii T, Kobayashi M, Takahashi K, et al. A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression. Biochem Biophys Res Commun. 2006;339:1076-82 pubmed
	Cognasse F, Hamzeh H, Chavarin P, Acquart S, Genin C, Garraud O. Evidence of Toll-like receptor molecules on human platelets. Immunol Cell Biol. 2005;83:196-8 pubmed
	Basak C, Pathak S, Bhattacharyya A, Mandal D, Pathak S, Kundu M. NF-kappaB- and C/EBPbeta-driven interleukin-1beta gene expression and PAK1-mediated caspase-1 activation play essential roles in interleukin-1beta release from Helicobacter pylori lipopolysaccharide-stimulated macrophages. J Biol Chem. 2005;280:4279-88 pubmed

product information

brand :

Novus

master code :

NB100-56723

SKU :

NB100-56723

product name :

TLR4 Antibody (HTA125) - BSA Free

description :

The TLR4 Antibody (HTA125) - BSA Free from Novus Biologicals is a mouse monoclonal antibody to TLR4. This antibody reacts with human, mouse, canine. The TLR4 Antibody (HTA125) - BSA Free has been validated for the following applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Functional, In vitro assay, Flow (Cell Surface), Immunohistochemistry Whole-Mount, CyTOF-ready, Block/Neutralize.

target :

TLR4

category :

Primary Antibodies

sizes available :

0.1 mg (also 0.025 mg)

buffer :

PBS

clonality :

Monoclonal

clone :

HTA125

concentration :

1.0 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

This TLR4 Antibody (HTA125) was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.

isotype :

IgG2a

purity :

Protein G purified

species :

Human, Mouse, Canine

theoretical molecular weight :

95.7 kDa

gene symbol :

TLR4

applications :

Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Functional, In vitro assay, Flow (Cell Surface), Immunohistochemistry Whole-Mount, CyTOF-ready, Block/Neutralize

USD :

356

USD 2023 :

379 USD

alt names :

ARMD10, CD_antigen: CD284, CD284, CD284 antigen, EC 3.2.2.6, EC:3.2.2.6, homolog of Drosophila toll, hta125, hta125 tlr4, hToll, TLR4, TLR-4, TOLL, toll like receptor 4 protein, toll-like receptor 4

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage