p73 Antibody (5B1288) - BSA Free | Novus Biologicals NB100-56674 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

p73 Antibody (5B1288) - BSA Free

catalog :

NB100-56674

quantity :

0.1 mg (also 0.025 mg)

price :

409 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5B1288

reactivity :

human, mouse, brewer's yeast

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

Novus Biologicals product webpage

citations: 61

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; fig e1 chromatin immunoprecipitation; mouse; fig e5	Venkatanarayan A, Raulji P, Norton W, Chakravarti D, Coarfa C, Su X, et al. IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo. Nature. 2015;517:626-30 pubmed publisher
western blot; brewer's yeast; loading ...; fig 5d	Billant O, LÃ©on A, Le Guellec S, Friocourt G, Blondel M, Voisset C. The dominant-negative interplay between p53, p63 and p73: A family affair. Oncotarget. 2016;7:69549-69564 pubmed publisher
western blot; human	Lu H, Yan C, Quan X, Yang X, Zhang J, Bian Y, et al. CK2 phosphorylates and inhibits TAp73 tumor suppressor function to promote expression of cancer stem cell genes and phenotype in head and neck cancer. Neoplasia. 2014;16:789-800 pubmed publisher
immunoprecipitation; mouse	King K, Reddi D, Ponnamperuma R, Gerdes M, Weinberg W. Dysregulated ?Np63? negatively regulates the maspin promoter in keratinocytes via blocking endogenous p73 binding. Mol Carcinog. 2014;53:698-710 pubmed publisher
immunohistochemistry; mouse	Lin Y, Sengupta S, Gurdziel K, Bell G, Jacks T, Flores E. p63 and p73 transcriptionally regulate genes involved in DNA repair. PLoS Genet. 2009;5:e1000680 pubmed publisher
western blot; human	Thottassery J, Westbrook L, Someya H, Parker W. c-Abl-independent p73 stabilization during gemcitabine- or 4'-thio-beta-D-arabinofuranosylcytosine-induced apoptosis in wild-type and p53-null colorectal cancer cells. Mol Cancer Ther. 2006;5:400-10 pubmed
western blot; human	Sayan A, Paradisi A, Vojtesek B, Knight R, Melino G, Candi E. New antibodies recognizing p73: comparison with commercial antibodies. Biochem Biophys Res Commun. 2005;330:186-93 pubmed
	Gao M, Liu H, Xiao Y, Guo Y, Wan X, Li X, et al. xCT regulates redox homeostasis and promotes photoreceptor survival after retinal detachment. Free Radic Biol Med. 2020;158:32-43 pubmed publisher
	Long Y, Tao H, Karachi A, Grippin A, Jin L, Chang Y, et al. Dysregulation of Glutamate Transport Enhances Treg Function That Promotes VEGF Blockade Resistance in Glioblastoma. Cancer Res. 2020;80:499-509 pubmed publisher
	Wu W, Sun H, Chen J, OuYang H, Yu X, Chen H, et al. Immunosuppressive Immature Myeloid Cell Generation Is Controlled by Glutamine Metabolism in Human Cancer. Cancer Immunol Res. 2019;7:1605-1618 pubmed publisher
	Pitman K, Alluri S, Kristian A, Aarnes E, Lyng H, Riss P, et al. Influx rate of 18F-fluoroaminosuberic acid reflects cystine/glutamate antiporter expression in tumour xenografts. Eur J Nucl Med Mol Imaging. 2019;46:2190-2198 pubmed publisher
	Isobe S, Kataoka M, Endo J, Moriyama H, Okazaki S, Tsuchihashi K, et al. Endothelial-Mesenchymal Transition Drives Expression of CD44 Variant and xCT in Pulmonary Hypertension. Am J Respir Cell Mol Biol. 2019;: pubmed publisher
	Ungard R, Linher Melville K, Nashed M, Sharma M, Wen J, Singh G. xCT knockdown in human breast cancer cells delays onset of cancer-induced bone pain. Mol Pain. 2019;15:1744806918822185 pubmed publisher
	Li C, Chen H, Lan Z, He S, Chen R, Wang F, et al. mTOR-dependent upregulation of xCT blocks melanin synthesis and promotes tumorigenesis. Cell Death Differ. 2019;: pubmed publisher
	Greenwood H, McCormick P, Gendron T, Glaser M, Pereira R, Maddocks O, et al. Measurement of Tumor Antioxidant Capacity and Prediction of Chemotherapy Resistance in Preclinical Models of Ovarian Cancer by Positron Emission Tomography. Clin Cancer Res. 2019;25:2471-2482 pubmed publisher
	Mi D, Dixit S, Warner T, Kennard J, Scharf D, Kessler E, et al. Altered glutamate clearance in ascorbate deficient mice increases seizure susceptibility and contributes to cognitive impairment in APP/PSEN1 mice. Neurobiol Aging. 2018;71:241-254 pubmed publisher
	Miladinovic T, Ungard R, Linher Melville K, Popovic S, Singh G. Functional effects of TrkA inhibition on system xC--mediated glutamate release and cancer-induced bone pain. Mol Pain. 2018;14:1744806918776467 pubmed publisher
	Hoehne A, James M, Alam I, Ronald J, Schneider B, D Souza A, et al. [18F]FSPG-PET reveals increased cystine/glutamate antiporter (xc-) activity in a mouse model of multiple sclerosis. J Neuroinflammation. 2018;15:55 pubmed publisher
	Bechard A, Hamor P, Schwendt M, Knackstedt L. The effects of ceftriaxone on cue-primed reinstatement of cocaine-seeking in male and female rats: estrous cycle effects on behavior and protein expression in the nucleus accumbens. Psychopharmacology (Berl). 2018;235:837-848 pubmed publisher
	Nasca C, Bigio B, Zelli D, De Angelis P, Lau T, Okamoto M, et al. Role of the Astroglial Glutamate Exchanger xCT in Ventral Hippocampus in Resilience to Stress. Neuron. 2017;96:402-413.e5 pubmed publisher
	Sayin V, LeBoeuf S, Singh S, Davidson S, Biancur D, Guzelhan B, et al. Activation of the NRF2 antioxidant program generates an imbalance in central carbon metabolism in cancer. elife. 2017;6: pubmed publisher
	Ng K, Chan L, Chai S, Tong M, Guan X, Lee N, et al. TP53INP1 Downregulation Activates a p73-Dependent DUSP10/ERK Signaling Pathway to Promote Metastasis of Hepatocellular Carcinoma. Cancer Res. 2017;77:4602-4612 pubmed publisher
	Bertaux Skeirik N, Wunderlich M, Teal E, Chakrabarti J, Biesiada J, Mahe M, et al. CD44 variant isoform 9 emerges in response to injury and contributes to the regeneration of the gastric epithelium. J Pathol. 2017;242:463-475 pubmed publisher
	Lacrosse A, O Donovan S, Sepulveda Orengo M, McCullumsmith R, Reissner K, Schwendt M, et al. Contrasting the Role of xCT and GLT-1 Upregulation in the Ability of Ceftriaxone to Attenuate the Cue-Induced Reinstatement of Cocaine Seeking and Normalize AMPA Receptor Subunit Expression. J Neurosci. 2017;37:5809-5821 pubmed publisher
	SuÃ¡rez Pozos E, MartÃnez Lozada Z, MÃ©ndez Flores O, Guillem A, HernÃ¡ndez Kelly L, CastelÃ¡n F, et al. Characterization of the cystine/glutamate antiporter in cultured Bergmann glia cells. Neurochem Int. 2017;108:52-59 pubmed publisher
	Martin C, Patel M, Melendez Ferro M, Sims B. Erythropoietin-induced cytoprotection in intestinal epithelial cells is linked to system Xc. Exp Cell Res. 2017;352:202-206 pubmed publisher
	Merckx E, Albertini G, Paterka M, Jensen C, Albrecht P, Dietrich M, et al. Absence of system xc- on immune cells invading the central nervous system alleviates experimental autoimmune encephalitis. J Neuroinflammation. 2017;14:9 pubmed publisher
	Kavanaugh G, Williams J, Morris A, Nickels M, Walker R, Koglin N, et al. Utility of [18F]FSPG PET to Image Hepatocellular Carcinoma: First Clinical Evaluation in a US Population. Mol Imaging Biol. 2016;18:924-934 pubmed
	Polewski M, Reveron Thornton R, Cherryholmes G, Marinov G, Cassady K, Aboody K. Increased Expression of System xc- in Glioblastoma Confers an Altered Metabolic State and Temozolomide Resistance. Mol Cancer Res. 2016;14:1229-1242 pubmed
	Linher Melville K, Nashed M, Ungard R, Haftchenary S, Rosa D, Gunning P, et al. Chronic Inhibition of STAT3/STAT5 in Treatment-Resistant Human Breast Cancer Cell Subtypes: Convergence on the ROS/SUMO Pathway and Its Effects on xCT Expression and System xc- Activity. PLoS ONE. 2016;11:e0161202 pubmed publisher
	Slosky L, Bassirirad N, Symons A, Thompson M, Doyle T, Forte B, et al. The cystine/glutamate antiporter system xc- drives breast tumor cell glutamate release and cancer-induced bone pain. Pain. 2016;157:2605-2616 pubmed
	Soria F, Zabala A, Pampliega O, Palomino A, Miguelez C, Ugedo L, et al. Cystine/glutamate antiporter blockage induces myelin degeneration. Glia. 2016;64:1381-95 pubmed publisher
	Pati D, Kelly K, Stennett B, Frazier C, Knackstedt L. Alcohol consumption increases basal extracellular glutamate in the nucleus accumbens core of Sprague-Dawley rats without increasing spontaneous glutamate release. Eur J Neurosci. 2016;44:1896-905 pubmed publisher
	Von Grabowiecki Y, Abreu P, Blanchard O, Palamiuc L, Benosman S, MÃ©riaux S, et al. Transcriptional activator TAp63 is upregulated in muscular atrophy during ALS and induces the pro-atrophic ubiquitin ligase Trim63. elife. 2016;5: pubmed publisher
	Van Liefferinge J, Bentea E, Demuyser T, Albertini G, Follin Arbelet V, Holmseth S, et al. Comparative analysis of antibodies to xCT (Slc7a11): Forewarned is forearmed. J Comp Neurol. 2016;524:1015-32 pubmed publisher
	Lu H, Samanta D, Xiang L, Zhang H, Hu H, Chen I, et al. Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype. Proc Natl Acad Sci U S A. 2015;112:E4600-9 pubmed publisher
	Merckx E, Demuyser T, Bentea E, Van Liefferinge J, Albertini G, Deneyer L, et al. Lack of effect of Theiler's murine encephalomyelitis virus infection on system xcâ». Neurosci Lett. 2015;593:124-8 pubmed publisher
	Rao P, Saternos H, Goodwani S, Sari Y. Effects of ceftriaxone on GLT1 isoforms, xCT and associated signaling pathways in P rats exposed to ethanol. Psychopharmacology (Berl). 2015;232:2333-42 pubmed publisher
	Aal Aaboda M, Alhaddad H, Osowik F, Nauli S, Sari Y. Effects of (R)-(-)-5-methyl-1-nicotinoyl-2-pyrazoline on glutamate transporter 1 and cysteine/glutamate exchanger as well as ethanol drinking behavior in male, alcohol-preferring rats. J Neurosci Res. 2015;93:930-7 pubmed publisher
	Martin L, Gardner L. Stress-induced inhibition of nonsense-mediated RNA decay regulates intracellular cystine transport and intracellular glutathione through regulation of the cystine/glutamate exchanger SLC7A11. Oncogene. 2015;34:4211-8 pubmed publisher
	Guerrieri F, Piconese S, Lacoste C, Schinzari V, Testoni B, Valogne Y, et al. The sodium/iodide symporter NIS is a transcriptional target of the p53-family members in liver cancer cells. Cell Death Dis. 2013;4:e807 pubmed publisher
	Du W, Jiang P, Mancuso A, Stonestrom A, Brewer M, Minn A, et al. TAp73 enhances the pentose phosphate pathway and supports cell proliferation. Nat Cell Biol. 2013;15:991-1000 pubmed publisher
	Niemantsverdriet M, Nagle P, Chiu R, Langendijk J, Kampinga H, Coppes R. ?Np73 enhances promoter activity of TGF-? induced genes. PLoS ONE. 2012;7:e50815 pubmed publisher
	Leonard M, Kommagani R, Payal V, Mayo L, Shamma H, Kadakia M. ?Np63? regulates keratinocyte proliferation by controlling PTEN expression and localization. Cell Death Differ. 2011;18:1924-33 pubmed publisher
	Rosenbluth J, Johnson K, Tang L, Triplett T, Pietenpol J. Evaluation of p63 and p73 antibodies for cross-reactivity. Cell Cycle. 2009;8:3702-6 pubmed
	Kommagani R, Whitlatch A, Leonard M, Kadakia M. p73 is essential for vitamin D-mediated osteoblastic differentiation. Cell Death Differ. 2010;17:398-407 pubmed publisher
	Rosenbluth J, Mays D, Pino M, Tang L, Pietenpol J. A gene signature-based approach identifies mTOR as a regulator of p73. Mol Cell Biol. 2008;28:5951-64 pubmed publisher
	Jones E, Dickman M, Whitmarsh A. Regulation of p73-mediated apoptosis by c-Jun N-terminal kinase. Biochem J. 2007;405:617-23 pubmed
	Limesand K, Schwertfeger K, Anderson S. MDM2 is required for suppression of apoptosis by activated Akt1 in salivary acinar cells. Mol Cell Biol. 2006;26:8840-56 pubmed
	Belloni L, Moretti F, Merlo P, Damalas A, Costanzo A, Blandino G, et al. DNp73alpha protects myogenic cells from apoptosis. Oncogene. 2006;25:3606-12 pubmed
	Lunghi P, Costanzo A, Salvatore L, Noguera N, Mazzera L, Tabilio A, et al. MEK1 inhibition sensitizes primary acute myelogenous leukemia to arsenic trioxide-induced apoptosis. Blood. 2006;107:4549-53 pubmed
	Accardi R, Dong W, Smet A, Cui R, Hautefeuille A, Gabet A, et al. Skin human papillomavirus type 38 alters p53 functions by accumulation of deltaNp73. EMBO Rep. 2006;7:334-40 pubmed
	Kulesz Martin M, Lagowski J, Fei S, Pelz C, Sears R, Powell M, et al. Melanocyte and keratinocyte carcinogenesis: p53 family protein activities and intersecting mRNA expression profiles. J Investig Dermatol Symp Proc. 2005;10:142-52 pubmed
	Tomasini R, Seux M, Nowak J, Bontemps C, Carrier A, Dagorn J, et al. TP53INP1 is a novel p73 target gene that induces cell cycle arrest and cell death by modulating p73 transcriptional activity. Oncogene. 2005;24:8093-104 pubmed
	Merlo P, Fulco M, Costanzo A, Mangiacasale R, Strano S, Blandino G, et al. A role of p73 in mitotic exit. J Biol Chem. 2005;280:30354-60 pubmed
	Papoutsaki M, Lanza M, Marinari B, Nistico S, Moretti F, Levrero M, et al. The p73 gene is an anti-tumoral target of the RARbeta/gamma-selective retinoid tazarotene. J Invest Dermatol. 2004;123:1162-8 pubmed
	Lunghi P, Costanzo A, Levrero M, Bonati A. Treatment with arsenic trioxide (ATO) and MEK1 inhibitor activates the p73-p53AIP1 apoptotic pathway in leukemia cells. Blood. 2004;104:519-25 pubmed
	Tullo A, Mastropasqua G, Bourdon J, Centonze P, Gostissa M, Costanzo A, et al. Adenosine deaminase, a key enzyme in DNA precursors control, is a new p73 target. Oncogene. 2003;22:8738-48 pubmed
	Fulco M, Costanzo A, Merlo P, Mangiacasale R, Strano S, Blandino G, et al. p73 is regulated by phosphorylation at the G2/M transition. J Biol Chem. 2003;278:49196-202 pubmed
	Vella V, Zhu J, Frasca F, Li C, Vigneri P, Vigneri R, et al. Exclusion of c-Abl from the nucleus restrains the p73 tumor suppression function. J Biol Chem. 2003;278:25151-7 pubmed
	Costanzo A, Merlo P, Pediconi N, Fulco M, Sartorelli V, Cole P, et al. DNA damage-dependent acetylation of p73 dictates the selective activation of apoptotic target genes. Mol Cell. 2002;9:175-86 pubmed

product information

master code :

NB100-56674

SKU :

NB100-56674

product name :

p73 Antibody (5B1288) - BSA Free

unit size :

0.1 mg (also 0.025 mg)

description :

The p73 Antibody (5B1288) - BSA Free from Novus is a mouse monoclonal antibody to p73. This antibody reacts with human,mouse. The p73 Antibody (5B1288) - BSA Free has been validated for the following applications: Chemotaxis,Co-Immunoprecipitation,IF/IHC,Immunoprecipitation,Western Blot,Immunocytochemistry/ Immunofluorescence,Chromatin Immunoprecipitation,Immunohistochemistry,Immunohistochemistry-Paraffin,Chromatin Immunoprecipitation (ChIP).

target :

p73

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

5B1288

concentration :

1.0 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

This antibody was raised against full-length human p73. The epitope is thought to lie around the center of the molecule (NP_005418).

isotype :

IgG1 Kappa

purity :

Protein G purified

species :

Human,Mouse

specificity :

It reacts with alpha, beta, gamma and delta isoforms of mouse and human p73 as well as the dominant negative p73 (Costanzo, et al, 2002)

theoretical molecular weight :

73 kDa

gene symbol :

TP73

accessionNumbers :

O15350

applications :

Chemotaxis,Co-Immunoprecipitation,IF/IHC,Immunoprecipitation,Western Blot,Immunocytochemistry/ Immunofluorescence,Chromatin Immunoprecipitation,Immunohistochemistry,Immunohistochemistry-Paraffin,Chromatin Immunoprecipitation (ChIP)

USD :

409 USD

alt names :

p53-related protein, P73p53-like transcription factor, TA p73, TAp73, tumor protein p73

storage :

Store at -20C. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage