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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
MyoD Antibody (5.8A) - BSA Free
catalog :
NB100-56511
quantity :
0.1 mg
price :
479 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5.8A
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation, immunocytochemistry knockout validation
more info or order :
citations: 54
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry knockout validation; mouse; 1:200; loading ...; fig s5g
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s7a
Xu L, Zhang M, Shi L, Yang X, Chen L, Cao N, et al. Neural stemness contributes to cell tumorigenicity. Cell Biosci. 2021;11:21 pubmed publisher
  • immunocytochemistry; mouse; 1:300; loading ...; fig 4a, 6e
Song R, Zhao S, Xu Y, Hu J, Ke S, Li F, et al. MRTF-A regulates myoblast commitment to differentiation by targeting PAX7 during muscle regeneration. J Cell Mol Med. 2021;25:8645-8661 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 4g
Millington G, Elliott K, Chang Y, Chang C, Dlugosz A, Brugmann S. Cilia-dependent GLI processing in neural crest cells is required for tongue development. Dev Biol. 2017;424:124-137 pubmed publisher
  • western blot; mouse
Chuang H, Hsiao K, Chang H, Wu C, Pan H. The homeobox transcription factor Irxl1 negatively regulates MyoD expression and myoblast differentiation. FEBS J. 2014;281:2990-3003 pubmed publisher
  • western blot; human
Sell H, Eckardt K, Taube A, Tews D, Gurgui M, van Echten Deckert G, et al. Skeletal muscle insulin resistance induced by adipocyte-conditioned medium: underlying mechanisms and reversibility. Am J Physiol Endocrinol Metab. 2008;294:E1070-7 pubmed publisher
  • immunoprecipitation; mouse
  • immunocytochemistry; mouse
  • western blot; mouse
Dias P, Parham D, Shapiro D, Tapscott S, Houghton P. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Res. 1992;52:6431-9 pubmed
Jo S, Park Y, Chang Y, Moon J, Lee S, Lee H, et al. Effects of the purified dry extract of fermented ginseng BST204 on muscle fiber regeneration. Biochem Biophys Rep. 2023;35:101525 pubmed publisher
Andre A, Rees K, O Connor S, Severson G, Newbern J, Wilson Rawls J, et al. Single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expression. Front Cell Dev Biol. 2023;11:1084068 pubmed publisher
Zhao T, Zhao R, Yi X, Cai R, Pang W. METTL3 promotes proliferation and myogenic differentiation through m6A RNA methylation/YTHDF1/2 signaling axis in myoblasts. Life Sci. 2022;298:120496 pubmed publisher
Chen L, Zhang M, Fang L, Yang X, Cao N, Xu L, et al. Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cells. J Biol Chem. 2021;297:101275 pubmed publisher
Peng Y, Xu M, Dou M, Shi X, Yang G, Li X. MicroRNA-129-5p inhibits C2C12 myogenesis and represses slow fiber gene expression in vitro. Am J Physiol Cell Physiol. 2021;320:C1031-C1041 pubmed publisher
Fang W, Tseng Y, Lee T, Fu Y, Chang W, Lo W, et al. Triptolide prevents LPS-induced skeletal muscle atrophy via inhibiting NF-κB/TNF-α and regulating protein synthesis/degradation pathway. Br J Pharmacol. 2021;178:2998-3016 pubmed publisher
Cai R, Zhang Q, Wang Y, Yong W, Zhao R, Pang W. Lnc-ORA interacts with microRNA-532-3p and IGF2BP2 to inhibit skeletal muscle myogenesis. J Biol Chem. 2021;296:100376 pubmed publisher
Takashima S, Usui S, Inoue O, Goten C, Yamaguchi K, Takeda Y, et al. Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells. Sci Rep. 2021;11:1520 pubmed publisher
Pacifici F, Della Morte D, Piermarini F, Arriga R, Scioli M, Capuani B, et al. Prdx6 Plays a Main Role in the Crosstalk Between Aging and Metabolic Sarcopenia. Antioxidants (Basel). 2020;9: pubmed publisher
De Micheli A, Laurilliard E, Heinke C, Ravichandran H, Fraczek P, Soueid Baumgarten S, et al. Single-Cell Analysis of the Muscle Stem Cell Hierarchy Identifies Heterotypic Communication Signals Involved in Skeletal Muscle Regeneration. Cell Rep. 2020;30:3583-3595.e5 pubmed publisher
Schüchner S, Behm C, Mudrak I, Ogris E. The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence context. Sci Signal. 2020;13: pubmed publisher
Pedrosa A, Bodrug N, Gómez Escudero J, Carter E, Reynolds L, Georgiou P, et al. Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861. Cancer Res. 2019;: pubmed publisher
Ida K, Miyamoto T, Higuchi S, Takeuchi H, Yamada S, Ono M, et al. Effectiveness of a genetic test panel designed for gynecological cancer: an exploratory study. Med Oncol. 2019;36:62 pubmed publisher
Morioka S, Perry J, Raymond M, Medina C, Zhu Y, Zhao L, et al. Efferocytosis induces a novel SLC program to promote glucose uptake and lactate release. Nature. 2018;563:714-718 pubmed publisher
Wu J, Fan W, Ma L, Geng X. miR-708-5p promotes fibroblast-like synoviocytes' cell apoptosis and ameliorates rheumatoid arthritis by the inhibition of Wnt3a/β-catenin pathway. Drug Des Devel Ther. 2018;12:3439-3447 pubmed publisher
Doğan S, Cerutti R, Benincá C, Brea Calvo G, Jacobs H, Zeviani M, et al. Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy. Cell Metab. 2018;28:764-775.e5 pubmed publisher
Li X, Zhu Y, Zhang H, Ma G, Wu G, Xiang A, et al. MicroRNA-106a-5p Inhibited C2C12 Myogenesis via Targeting PIK3R1 and Modulating the PI3K/AKT Signaling. Genes (Basel). 2018;9: pubmed publisher
Rodríguez Muela N, Parkhitko A, Grass T, Gibbs R, Norabuena E, Perrimon N, et al. Blocking p62-dependent SMN degradation ameliorates spinal muscular atrophy disease phenotypes. J Clin Invest. 2018;128:3008-3023 pubmed publisher
Jheng J, Chen Y, Ao U, Chan D, Huang J, Hung K, et al. The double-edged sword of endoplasmic reticulum stress in uremic sarcopenia through myogenesis perturbation. J Cachexia Sarcopenia Muscle. 2018;9:570-584 pubmed publisher
Waldemer Streyer R, Reyes Ordoñez A, Kim D, Zhang R, Singh N, Chen J. Cxcl14 depletion accelerates skeletal myogenesis by promoting cell cycle withdrawal. NPJ Regen Med. 2017;2: pubmed publisher
Mahadevan S, Sathappan V, Utama B, Lorenzo I, Kaskar K, Van den Veyver I. Maternally expressed NLRP2 links the subcortical maternal complex (SCMC) to fertility, embryogenesis and epigenetic reprogramming. Sci Rep. 2017;7:44667 pubmed publisher
van der Kant R, Jonker C, Wijdeven R, Bakker J, Janssen L, Klumperman J, et al. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity. J Biol Chem. 2015;290:30280-90 pubmed publisher
da Costa J, Carvalhais V, Amado F, Silva A, Nogueira Ferreira R, Ferreira R, et al. Anti-tumoral activity of human salivary peptides. Peptides. 2015;71:170-8 pubmed publisher
Jang K, Mano H, Aoki K, Hayashi T, Muto A, Nambu Y, et al. Mitochondrial function provides instructive signals for activation-induced B-cell fates. Nat Commun. 2015;6:6750 pubmed publisher
Iampietro M, Morissette G, Gravel A, Flamand L. Inhibition of interleukin-2 gene expression by human herpesvirus 6B U54 tegument protein. J Virol. 2014;88:12452-63 pubmed publisher
Pekkurnaz G, Trinidad J, Wang X, Kong D, Schwarz T. Glucose regulates mitochondrial motility via Milton modification by O-GlcNAc transferase. Cell. 2014;158:54-68 pubmed publisher
Barnett T, Liebl D, Seymour L, Gillen C, Lim J, LaRock C, et al. The globally disseminated M1T1 clone of group A Streptococcus evades autophagy for intracellular replication. Cell Host Microbe. 2013;14:675-82 pubmed publisher
Hsieh Y, Chou M, Chen H, Tseng J. IMP1 promotes choriocarcinoma cell migration and invasion through the novel effectors RSK2 and PPME1. Gynecol Oncol. 2013;131:182-90 pubmed publisher
Hubbi M, Hu H, Kshitiz -, Gilkes D, Semenza G. Sirtuin-7 inhibits the activity of hypoxia-inducible factors. J Biol Chem. 2013;288:20768-75 pubmed publisher
Megill A, Lee T, Dibattista A, Song J, Spitzer M, Rubinshtein M, et al. A tetra(ethylene glycol) derivative of benzothiazole aniline enhances Ras-mediated spinogenesis. J Neurosci. 2013;33:9306-18 pubmed publisher
Wang H, Shi L, Wong C, Han X, Hwang P, Truong L, et al. The interaction of CtIP and Nbs1 connects CDK and ATM to regulate HR-mediated double-strand break repair. PLoS Genet. 2013;9:e1003277 pubmed publisher
Lu C, Truong L, Aslanian A, Shi L, Li Y, Hwang P, et al. The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination. J Biol Chem. 2012;287:43984-94 pubmed publisher
Santini E, Feyder M, Gangarossa G, Bateup H, Greengard P, Fisone G. Dopamine- and cAMP-regulated phosphoprotein of 32-kDa (DARPP-32)-dependent activation of extracellular signal-regulated kinase (ERK) and mammalian target of rapamycin complex 1 (mTORC1) signaling in experimental parkinsonism. J Biol Chem. 2012;287:27806-12 pubmed publisher
Wang H, Shao Z, Shi L, Hwang P, Truong L, Berns M, et al. CtIP protein dimerization is critical for its recruitment to chromosomal DNA double-stranded breaks. J Biol Chem. 2012;287:21471-80 pubmed publisher
Zhao Y, Wang Y, Hu J, Zhang X, Zhang Y. CutA divalent cation tolerance homolog (Escherichia coli) (CUTA) regulates ?-cleavage of ?-amyloid precursor protein (APP) through interacting with ?-site APP cleaving protein 1 (BACE1). J Biol Chem. 2012;287:11141-50 pubmed publisher
Majmundar A, Skuli N, Mesquita R, Kim M, Yodh A, Nguyen McCarty M, et al. O(2) regulates skeletal muscle progenitor differentiation through phosphatidylinositol 3-kinase/AKT signaling. Mol Cell Biol. 2012;32:36-49 pubmed publisher
Kim M, Jung J, Mo J, Ann E, Ahn J, Yoon J, et al. The intracellular domain of Jagged-1 interacts with Notch1 intracellular domain and promotes its degradation through Fbw7 E3 ligase. Exp Cell Res. 2011;317:2438-46 pubmed publisher
Hubbi M, Luo W, Baek J, Semenza G. MCM proteins are negative regulators of hypoxia-inducible factor 1. Mol Cell. 2011;42:700-12 pubmed publisher
Hosoyama T, Nishijo K, Prajapati S, Li G, Keller C. Rb1 gene inactivation expands satellite cell and postnatal myoblast pools. J Biol Chem. 2011;286:19556-64 pubmed publisher
Murri Plesko M, Hulikova A, Oosterwijk E, Scott A, Zortea A, Harris A, et al. Antibody inhibiting enzymatic activity of tumour-associated carbonic anhydrase isoform IX. Eur J Pharmacol. 2011;657:173-83 pubmed publisher
Bernardi H, Gay S, Fedon Y, Vernus B, Bonnieu A, Bacou F. Wnt4 activates the canonical ?-catenin pathway and regulates negatively myostatin: functional implication in myogenesis. Am J Physiol Cell Physiol. 2011;300:C1122-38 pubmed publisher
Sun Y, Ge Y, Drnevich J, Zhao Y, Band M, Chen J. Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal myogenesis. J Cell Biol. 2010;189:1157-69 pubmed publisher
Gillespie M, Le Grand F, Scime A, Kuang S, von Maltzahn J, Seale V, et al. p38-{gamma}-dependent gene silencing restricts entry into the myogenic differentiation program. J Cell Biol. 2009;187:991-1005 pubmed publisher
Xavier C, Rastetter R, Stumpf M, Rosentreter A, Muller R, Reimann J, et al. Structural and functional diversity of novel coronin 1C (CRN2) isoforms in muscle. J Mol Biol. 2009;393:287-99 pubmed publisher
Silvestri L, Pagani A, Camaschella C. Furin-mediated release of soluble hemojuvelin: a new link between hypoxia and iron homeostasis. Blood. 2008;111:924-31 pubmed
Trinkle Mulcahy L, Chusainow J, Lam Y, Swift S, Lamond A. Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions. Methods Mol Biol. 2007;365:133-54 pubmed
Bergman M, Teerlink J, Mahimkar R, Li L, Zhu B, Nguyen A, et al. Cardiac matrix metalloproteinase-2 expression independently induces marked ventricular remodeling and systolic dysfunction. Am J Physiol Heart Circ Physiol. 2007;292:H1847-60 pubmed
Hilpert K, Hansen G, Wessner H, Kuttner G, Welfle K, Seifert M, et al. Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein Eng. 2001;14:803-6 pubmed
product information
master code :
NB100-56511
SKU :
NB100-56511
product name :
MyoD Antibody (5.8A) - BSA Free
unit size :
0.1 mg
description :
The MyoD Antibody (5.8A) - BSA Free from Novus is a mouse monoclonal antibody to MyoD. This antibody reacts with human,mouse. The MyoD Antibody (5.8A) - BSA Free has been validated for the following applications: Immunoprecipitation,Western Blot,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry,IF/IHC,CyTOF-ready,Flow Cytometry,Immunohistochemistry-Paraffin,Knockdown Validated,Immunohistochemistry-Frozen,Knockout Validated.
target :
MyoD
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
5.8A
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
The 5.8A antibody was made against recombinant mouse MyoD protein but it also recognizes human (myf3), rat, and cat homologs. The epitope of this antibody was mapped to a region within aa 180-189 of mouse MyoD (NP_002469).
isotype :
IgG1 Kappa
purity :
Protein G purified
species :
Human,Mouse
specificity :
In Rh-30, a ~45 kDa band should be observed
gene symbol :
MYOD1
Antibody validation :
Biological Validation
accessionNumbers :
P15172, P10085
applications :
Immunohistochemistry,Western Blot,Immunocytochemistry/ Immunofluorescence,IF/IHC,CyTOF-ready,Flow Cytometry,Immunoprecipitation,Immunohistochemistry-Paraffin,Knockdown Validated,Immunohistochemistry-Frozen,Knockout Validated
USD :
479 USD
alt names :
bHLHc1BHLHC1, Class C basic helix-loop-helix protein 1, MYF3Myf-3, MYODmyoblast determination protein 1, myogenic differentiation 1, Myogenic factor 3myf-3, PUM
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.