IKK beta Antibody (10AG2) - BSA Free | Novus Biologicals NB100-56509 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

IKK beta Antibody (10AG2) - BSA Free

catalog :

NB100-56509

quantity :

0.1 mg (also 0.025 mg)

price :

409 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10AG2

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Novus Biologicals product webpage

citations: 57

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:75; loading ...; fig s2a	Reid M, Lowman X, Pan M, Tran T, Warmoes M, Ishak Gabra M, et al. IKKÎ² promotes metabolic adaptation to glutamine deprivation via phosphorylation and inhibition of PFKFB3. Genes Dev. 2016;30:1837-51 pubmed publisher
immunoprecipitation; human western blot; human	Buhrmann C, Büsch F, Shayan P, Shakibaei M. Sirtuin-1 (SIRT1) is required for promoting chondrogenic differentiation of mesenchymal stem cells. J Biol Chem. 2014;289:22048-62 pubmed publisher
western blot; mouse	Das U, Manna K, Sinha M, Datta S, Das D, Chakraborty A, et al. Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS ONE. 2014;9:e97599 pubmed publisher
immunocytochemistry; mouse	Paul S, Traver M, Kashyap A, Washington M, Latoche J, Schaefer B. T cell receptor signals to NF-κB are transmitted by a cytosolic p62-Bcl10-Malt1-IKK signalosome. Sci Signal. 2014;7:ra45 pubmed publisher
western blot; human	Breuer R, Becker M, Brechmann M, Mock T, Arnold R, Krammer P. The protein phosphatase 2A regulatory subunit B56? mediates suppression of T cell receptor (TCR)-induced nuclear factor-?B (NF-?B) activity. J Biol Chem. 2014;289:14996-5004 pubmed publisher
western blot; mouse	Gray C, Remouchamps C, McCorkell K, Solt L, Dejardin E, Orange J, et al. Noncanonical NF-?B signaling is limited by classical NF-?B activity. Sci Signal. 2014;7:ra13 pubmed publisher
western blot; human	Ren T, Takahashi Y, Liu X, Loughran T, Sun S, Wang H, et al. HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains. Oncogene. 2015;34:334-45 pubmed publisher
western blot; human	Bimonte S, Barbieri A, Palma G, Luciano A, Rea D, Arra C. Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer. Biomed Res Int. 2013;2013:810423 pubmed publisher
western blot; mouse	Page A, Cascallana J, Casanova M, Navarro M, Alameda J, Perez P, et al. IKK? overexpression leads to pathologic lesions in stratified epithelia and exocrine glands and to tumoral transformation of oral epithelia. Mol Cancer Res. 2011;9:1329-38 pubmed publisher
immunocytochemistry; mouse	Koch K, Maeda S, He G, Karin M, Leffert H. Targeted deletion of hepatocyte Ikkbeta confers growth advantages. Biochem Biophys Res Commun. 2009;380:349-54 pubmed publisher
western blot; human	Huang J, Ren T, Guan H, Jiang Y, Cheng H. HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaB. J Biol Chem. 2009;284:6208-17 pubmed publisher
western blot; human	Pandey M, Sung B, Kunnumakkara A, Sethi G, Chaturvedi M, Aggarwal B. Berberine modifies cysteine 179 of IkappaBalpha kinase, suppresses nuclear factor-kappaB-regulated antiapoptotic gene products, and potentiates apoptosis. Cancer Res. 2008;68:5370-9 pubmed publisher
western blot; human	Kasinski A, Du Y, Thomas S, Zhao J, Sun S, Khuri F, et al. Inhibition of IkappaB kinase-nuclear factor-kappaB signaling pathway by 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24), a novel monoketone analog of curcumin. Mol Pharmacol. 2008;74:654-61 pubmed publisher
immunoprecipitation; mouse	Beraza N, Ludde T, Assmus U, Roskams T, Vander Borght S, Trautwein C. Hepatocyte-specific IKK gamma/NEMO expression determines the degree of liver injury. Gastroenterology. 2007;132:2504-17 pubmed
western blot; mouse	Herrmann O, Baumann B, De Lorenzi R, Muhammad S, Zhang W, Kleesiek J, et al. IKK mediates ischemia-induced neuronal death. Nat Med. 2005;11:1322-9 pubmed
western blot; human	Shishodia S, Aggarwal B. Guggulsterone inhibits NF-kappaB and IkappaBalpha kinase activation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis. J Biol Chem. 2004;279:47148-58 pubmed
western blot; human	Takada Y, Singh S, Aggarwal B. Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis. J Biol Chem. 2004;279:15096-104 pubmed
western blot; mouse	Leis H, Page A, Ramirez A, Bravo A, Segrelles C, Paramio J, et al. Glucocorticoid Receptor Counteracts Tumorigenic Activity of Akt in Skin through Interference with the Phosphatidylinositol 3-Kinase Signaling Pathway. Mol Endocrinol. 2004;18:303-11 pubmed
western blot; mouse	Santos M, Perez P, Segrelles C, Ruiz S, Jorcano J, Paramio J. Impaired NF-kappa B activation and increased production of tumor necrosis factor alpha in transgenic mice expressing keratin K10 in the basal layer of the epidermis. J Biol Chem. 2003;278:13422-30 pubmed
	McCorkell K, Jayachandran N, Cully M, Freund Brown J, Weinkopff T, Monslow J, et al. Lymph node formation and B cell homeostasis require IKK-α in distinct endothelial cell-derived compartments. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
	Huo Y, Mijiti A, Cai R, Gao Z, Aini M, Mijiti A, et al. Scutellarin alleviates type 2 diabetes (HFD/low dose STZ)-induced cardiac injury through modulation of oxidative stress, inflammation, apoptosis and fibrosis in mice. Hum Exp Toxicol. 2021;40:S460-S474 pubmed publisher
	Karim K, Giribabu N, Salleh N. Marantodes pumilum Var Alata (Kacip Fatimah) ameliorates derangement in RANK/RANKL/OPG pathway and reduces inflammation and oxidative stress in the bone of estrogen-deficient female rats with type-2 diabetes. Phytomedicine. 2021;91:153677 pubmed publisher
	Best K, Nichols A, Knapp E, Hammert W, Ketonis C, Jonason J, et al. NF-κB activation persists into the remodeling phase of tendon healing and promotes myofibroblast survival. Sci Signal. 2020;13: pubmed publisher
	Rahtes A, Li L. Polarization of Low-Grade Inflammatory Monocytes Through TRAM-Mediated Up-Regulation of Keap1 by Super-Low Dose Endotoxin. Front Immunol. 2020;11:1478 pubmed publisher
	Sun L, Lin P, Chen Y, Yu H, Ren S, Wang J, et al. miR-182-3p/Myadm contribute to pulmonary artery hypertension vascular remodeling via a KLF4/p21-dependent mechanism. Theranostics. 2020;10:5581-5599 pubmed publisher
	De S, Karim F, Kiessu E, Cushing L, Lin L, Ghandil P, et al. Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling. J Biol Chem. 2018;293:15208-15220 pubmed publisher
	Abdi K, Lai C, Paez Gonzalez P, Lay M, Pyun J, Kuo C. Uncovering inherent cellular plasticity of multiciliated ependyma leading to ventricular wall transformation and hydrocephalus. Nat Commun. 2018;9:1655 pubmed publisher
	Kirkley K, Walton K, Duncan C, Tjalkens R. Spontaneous Development of Cutaneous Squamous Cell Carcinoma in Mice with Cell-specific Deletion of Inhibitor of κB Kinase 2. Comp Med. 2017;67:407-415 pubmed
	Cleary M, Mansoor A, Settelmeyer T, Ijiri Y, Ladner K, Svalina M, et al. NF?B signaling in alveolar rhabdomyosarcoma. Dis Model Mech. 2017;10:1109-1115 pubmed publisher
	Liu J, Ibi D, Taniguchi K, Lee J, Herrema H, Akosman B, et al. Inflammation Improves Glucose Homeostasis through IKKÎ²-XBP1s Interaction. Cell. 2016;167:1052-1066.e18 pubmed publisher
	Delhase M, Kim S, Lee H, Naiki Ito A, Chen Y, Ahn E, et al. TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2. Proc Natl Acad Sci U S A. 2012;109:E177-86 pubmed publisher
	Harikumar K, Sung B, Pandey M, Guha S, Krishnan S, Aggarwal B. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway. Mol Pharmacol. 2010;77:818-27 pubmed publisher
	Jin H, Lee D, Kim D, Chung J, Lee S, Lee T. cIAP1, cIAP2, and XIAP act cooperatively via nonredundant pathways to regulate genotoxic stress-induced nuclear factor-kappaB activation. Cancer Res. 2009;69:1782-91 pubmed publisher
	Lamothe B, Webster W, Gopinathan A, Besse A, Campos A, Darnay B. TRAF6 ubiquitin ligase is essential for RANKL signaling and osteoclast differentiation. Biochem Biophys Res Commun. 2007;359:1044-9 pubmed
	Vunta H, Davis F, Palempalli U, Bhat D, Arner R, Thompson J, et al. The anti-inflammatory effects of selenium are mediated through 15-deoxy-Delta12,14-prostaglandin J2 in macrophages. J Biol Chem. 2007;282:17964-73 pubmed
	Leis H, Sanchis A, Perez P. Deletion of the N-terminus of IKKgamma induces apoptosis in keratinocytes and impairs the AKT/PTEN signaling pathway. Exp Cell Res. 2007;313:742-52 pubmed
	Jackson Bernitsas D, Ichikawa H, Takada Y, Myers J, Lin X, Darnay B, et al. Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma. Oncogene. 2007;26:1385-97 pubmed
	Sethi G, Ahn K, Sandur S, Lin X, Chaturvedi M, Aggarwal B. Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway. J Biol Chem. 2006;281:23425-35 pubmed
	Sandur S, Ichikawa H, Sethi G, Ahn K, Aggarwal B. Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) suppresses NF-kappaB activation and NF-kappaB-regulated gene products through modulation of p65 and IkappaBalpha kinase activation, leading to potentiation of apoptosis induced by cytokine and chemoth. J Biol Chem. 2006;281:17023-33 pubmed
	Ichikawa H, Aggarwal B. Guggulsterone inhibits osteoclastogenesis induced by receptor activator of nuclear factor-kappaB ligand and by tumor cells by suppressing nuclear factor-kappaB activation. Clin Cancer Res. 2006;12:662-8 pubmed
	Nenci A, Huth M, Funteh A, Schmidt Supprian M, Bloch W, Metzger D, et al. Skin lesion development in a mouse model of incontinentia pigmenti is triggered by NEMO deficiency in epidermal keratinocytes and requires TNF signaling. Hum Mol Genet. 2006;15:531-42 pubmed
	Takada Y, Gillenwater A, Ichikawa H, Aggarwal B. Suberoylanilide hydroxamic acid potentiates apoptosis, inhibits invasion, and abolishes osteoclastogenesis by suppressing nuclear factor-kappaB activation. J Biol Chem. 2006;281:5612-22 pubmed
	Shishodia S, Gutierrez A, Lotan R, Aggarwal B. N-(4-hydroxyphenyl)retinamide inhibits invasion, suppresses osteoclastogenesis, and potentiates apoptosis through down-regulation of I(kappa)B(alpha) kinase and nuclear factor-kappaB-regulated gene products. Cancer Res. 2005;65:9555-65 pubmed
	Shishodia S, Amin H, Lai R, Aggarwal B. Curcumin (diferuloylmethane) inhibits constitutive NF-kappaB activation, induces G1/S arrest, suppresses proliferation, and induces apoptosis in mantle cell lymphoma. Biochem Pharmacol. 2005;70:700-13 pubmed
	Ichikawa H, Takada Y, Murakami A, Aggarwal B. Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products. J Immunol. 2005;174:7383-92 pubmed
	Luedde T, Assmus U, Wüstefeld T, Meyer zu Vilsendorf A, Roskams T, Schmidt Supprian M, et al. Deletion of IKK2 in hepatocytes does not sensitize these cells to TNF-induced apoptosis but protects from ischemia/reperfusion injury. J Clin Invest. 2005;115:849-59 pubmed
	Shishodia S, Koul D, Aggarwal B. Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF-induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma: correlation with suppression of COX-2 synthesis. J Immunol. 2004;173:2011-22 pubmed
	Takada Y, Fang X, Jamaluddin M, Boyd D, Aggarwal B. Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor. J Biol Chem. 2004;279:39541-54 pubmed
	Takada Y, Khuri F, Aggarwal B. Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated gene expression and up-regulation of apoptosis. J Biol Chem. 2004;279:26287-99 pubmed
	Takada Y, Aggarwal B. Flavopiridol inhibits NF-kappaB activation induced by various carcinogens and inflammatory agents through inhibition of IkappaBalpha kinase and p65 phosphorylation: abrogation of cyclin D1, cyclooxygenase-2, and matrix metalloprotease-9. J Biol Chem. 2004;279:4750-9 pubmed
	Majumdar S, Aggarwal B. Adenosine suppresses activation of nuclear factor-kappaB selectively induced by tumor necrosis factor in different cell types. Oncogene. 2003;22:1206-18 pubmed
	Ashikawa K, Majumdar S, Banerjee S, Bharti A, Shishodia S, Aggarwal B. Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. J Immunol. 2002;169:6490-7 pubmed
	Anto R, Mukhopadhyay A, Shishodia S, Gairola C, Aggarwal B. Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2. Carcinogenesis. 2002;23:1511-8 pubmed
	Majumdar S, Lamothe B, Aggarwal B. Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester. J Immunol. 2002;168:2644-51 pubmed
	Majumdar S, Aggarwal B. Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation. J Immunol. 2001;167:2911-20 pubmed
	Miller B, Zandi E. Complete reconstitution of human IkappaB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKgamma on the complex activity in the absence of stimulation. J Biol Chem. 2001;276:36320-6 pubmed
	Manna S, Mukhopadhyay A, Aggarwal B. Leflunomide suppresses TNF-induced cellular responses: effects on NF-kappa B, activator protein-1, c-Jun N-terminal protein kinase, and apoptosis. J Immunol. 2000;165:5962-9 pubmed

product information

master code :

NB100-56509

SKU :

NB100-56509

product name :

IKK beta Antibody (10AG2) - BSA Free

unit size :

0.1 mg (also 0.025 mg)

description :

The IKK beta Antibody (10AG2) - BSA Free from Novus is a mouse monoclonal antibody to IKK beta. This antibody reacts with human,mouse,rat. The IKK beta Antibody (10AG2) - BSA Free has been validated for the following applications: IF/IHC,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Flow Cytometry,Immunohistochemistry,Immunoblotting,Simple Western,CyTOF-ready.

target :

IKK beta

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

10AG2

concentration :

1.0 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

Full-length recombinant human IKK beta protein (NP_001547).

isotype :

IgG1

purity :

Protein G purified

species :

Human,Mouse,Rat

theoretical molecular weight :

87 kDa

gene symbol :

IKBKB

Antibody validation :

Biological Validation

accessionNumbers :

O14920

applications :

IF/IHC,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Flow Cytometry,Immunohistochemistry,Immunoblotting,Simple Western,CyTOF-ready

USD :

409 USD

alt names :

EC 2.7.11, EC 2.7.11.10, I-kappa-B kinase 2, I-kappa-B-kinase beta, IkBKB, IKK2FLJ38368, IKK-B, IKK-betaFLJ36218, IKKBFLJ40509, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta, inhibitor of nuclear factor kappa-B kinase subunit beta, MGC131801, NFKBIKBFLJ33771, Nuclear factor NF-kappa-B inhibitor kinase beta

storage :

Store at -20C. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage