Cytochrome c Antibody (7H8.2C12) - BSA Free | Novus Biologicals NB100-56503 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Cytochrome c Antibody (7H8.2C12) - BSA Free

catalog :

NB100-56503

quantity :

0.1 mg (also 0.025 mg)

price :

459 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H8.2C12

reactivity :

fruit fly , human, mouse, rat, fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Novus Biologicals product webpage

citations: 61

Published Application/Species/Sample/Dilution	Reference
western blot; fruit fly ; loading ...; fig 3c	Zhang Y, Chen Y, Gucek M, Xu H. The mitochondrial outer membrane protein MDI promotes local protein synthesis and mtDNA replication. EMBO J. 2016;35:1045-57 pubmed publisher
western blot; human	Dhumale S, Waghela B, Pathak C. Quercetin protects necrotic insult and promotes apoptosis by attenuating the expression of RAGE and its ligand HMGB1 in human breast adenocarcinoma cells. IUBMB Life. 2015;67:361-73 pubmed publisher
western blot; human	Waghela B, Sharma A, Dhumale S, Pandey S, Pathak C. Curcumin conjugated with PLGA potentiates sustainability, anti-proliferative activity and apoptosis in human colon carcinoma cells. PLoS ONE. 2015;10:e0117526 pubmed publisher
western blot; human	Nogami K, Takahama K, Okushima A, Oyoshi T, Fujimoto K, Inouye M. Tailor-made designer helical peptides that induce mitochondrion-mediated cell death without necrosis. Chembiochem. 2014;15:2571-6 pubmed publisher
western blot; mouse	Sagar S, Kumar P, Behera R, Pal A. Effects of CEES and LPS synergistically stimulate oxidative stress inactivates OGG1 signaling in macrophage cells. J Hazard Mater. 2014;278:236-49 pubmed publisher
western blot; rat	Kumar P, Rao G, Pal B, Pal A. Hyperglycemia-induced oxidative stress induces apoptosis by inhibiting PI3-kinase/Akt and ERK1/2 MAPK mediated signaling pathway causing downregulation of 8-oxoG-DNA glycosylase levels in glial cells. Int J Biochem Cell Biol. 2014;53:302-19 pubmed publisher
western blot; mouse	Wang J, Schilling J, Niesman I, Headrick J, Finley J, Kwan E, et al. Cardioprotective trafficking of caveolin to mitochondria is Gi-protein dependent. Anesthesiology. 2014;121:538-48 pubmed publisher
western blot; mouse western blot; human	Pradhan N, Pratheek B, Garai A, Kumar A, Meena V, Ghosh S, et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol Int. 2014;38:1118-31 pubmed publisher
western blot; human	Zhang W, Wang X, Chen T. Resveratrol induces apoptosis via a Bak-mediated intrinsic pathway in human lung adenocarcinoma cells. Cell Signal. 2012;24:1037-46 pubmed publisher
immunocytochemistry; mouse	Douglas R, Ryu J, Kanaan A, Del Carmen Rivero M, Dugan L, Haddad G, et al. Neuronal death during combined intermittent hypoxia/hypercapnia is due to mitochondrial dysfunction. Am J Physiol Cell Physiol. 2010;298:C1594-602 pubmed publisher
western blot; mouse	Zhu C, Xu F, Wang X, Shibata M, Uchiyama Y, Blomgren K, et al. Different apoptotic mechanisms are activated in male and female brains after neonatal hypoxia-ischaemia. J Neurochem. 2006;96:1016-27 pubmed
western blot; fruit fly	Wong S, Chen Y, Chan C, Chan C, Chan P, Chui Y, et al. In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila. Biochem Biophys Res Commun. 2005;337:720-9 pubmed
western blot; human	Liu C, Lee C, Hong C, Wei Y. Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis. Ann N Y Acad Sci. 2004;1011:133-45 pubmed
western blot; rat	Zhu C, Wang X, Cheng X, Qiu L, Xu F, Simbruner G, et al. Post-ischemic hypothermia-induced tissue protection and diminished apoptosis after neonatal cerebral hypoxia-ischemia. Brain Res. 2004;996:67-75 pubmed
western blot; rat	Pervaiz S, Seyed M, Hirpara J, Clement M, Loh K. Purified photoproducts of merocyanine 540 trigger cytochrome C release and caspase 8-dependent apoptosis in human leukemia and melanoma cells. Blood. 1999;93:4096-108 pubmed
western blot; rat	Fujimura M, Morita Fujimura Y, Murakami K, Kawase M, Chan P. Cytosolic redistribution of cytochrome c after transient focal cerebral ischemia in rats. J Cereb Blood Flow Metab. 1998;18:1239-47 pubmed
western blot; rat	Neame S, Rubin L, Philpott K. Blocking cytochrome c activity within intact neurons inhibits apoptosis. J Cell Biol. 1998;142:1583-93 pubmed
	Li S, Chen X, Guan S, Wang Z, Cao W, Luo G, et al. Precisely Amplifying Intracellular Oxidative Storm by Metal-Organic Coordination Polymers to Augment Anticancer Immunity. ACS Nano. 2023;17:15165-15179 pubmed publisher
	Oikawa R, Watanabe Y, Yotsuyanagi H, Yamamoto H, Itoh F. DNA methylation at hepatitis B virus integrants and flanking host mitochondrially encoded cytochrome C oxidase III. Oncol Lett. 2022;24:424 pubmed publisher
	Wang Y, Liao J, Chen K, Su H, Liu P, Chen Y, et al. Lumbrokinase regulates endoplasmic reticulum stress to improve neurological deficits in ischemic stroke. Neuropharmacology. 2022;221:109277 pubmed publisher
	Zhao S, Chen W, Li W, Yu W, Li S, Rao T, et al. LncRNA TUG1 attenuates ischaemia-reperfusion-induced apoptosis of renal tubular epithelial cells by sponging miR-144-3p via targeting Nrf2. J Cell Mol Med. 2021;25:9767-9783 pubmed publisher
	Liu Z, Liu Q, Zhang B, Liu Q, Fang L, Gou S. Blood-Brain Barrier Permeable and NO-Releasing Multifunctional Nanoparticles for Alzheimer's Disease Treatment: Targeting NO/cGMP/CREB Signaling Pathways. J Med Chem. 2021;64:13853-13872 pubmed publisher
	Hu F, Wu Y, Liu C, Zhu Y, Ye S, Xi Y, et al. Penicillin disrupts mitochondrial function and induces autophagy in colorectal cancer cell lines. Oncol Lett. 2021;22:691 pubmed publisher
	Deng X, Yang P, Gao T, Liu M, Li X. Allicin attenuates myocardial apoptosis, inflammation and mitochondrial injury during hypoxia-reoxygenation: an in vitro study. BMC Cardiovasc Disord. 2021;21:200 pubmed publisher
	Nam Y, Kong D, Wang D, Orfali R, Sherpa R, Totonchy J, et al. Differential modulation of SK channel subtypes by phosphorylation. Cell Calcium. 2021;94:102346 pubmed publisher
	Yoon J, Shin J, Pham T, Choi Y, Ryu H, Oh S, et al. Methyl lucidone induces apoptosis and G2/M phase arrest via the PI3K/Akt/NF-κB pathway in ovarian cancer cells. Pharm Biol. 2020;58:51-59 pubmed publisher
	Rizwan H, Pal S, Sabnam S, Pal A. High glucose augments ROS generation regulates mitochondrial dysfunction and apoptosis via stress signalling cascades in keratinocytes. Life Sci. 2020;241:117148 pubmed publisher
	Saladini S, Aventaggiato M, Barreca F, Morgante E, Sansone L, Russo M, et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells. 2019;8: pubmed publisher
	Zhao S, Li W, Cheng F, Rao T, Yu W, Ruan Y, et al. High-pressure carbon dioxide pneumoperitoneum induces oxidative stress and mitochondria-associated apoptotic pathway in rabbit kidneys with severe hydronephrosis. Int J Mol Med. 2019;43:305-315 pubmed publisher
	Pennington E, Sullivan E, Fix A, Dadoo S, Zeczycki T, DeSantis A, et al. Proteolipid domains form in biomimetic and cardiac mitochondrial vesicles and are regulated by cardiolipin concentration but not monolyso-cardiolipin. J Biol Chem. 2018;293:15933-15946 pubmed publisher
	Li W, Zhao S, Cheng F, Rao T, Yu W, Ruan Y, et al. Oxidative damage and mitochondrial injuries differ following pneumoperitoneum pressure in rabbit models of varying degrees of hydronephrosis. Mol Med Rep. 2018;17:6819-6827 pubmed publisher
	Marycz K, Kornicka K, Marędziak M, Golonka P, Nicpoń J. Equine metabolic syndrome impairs adipose stem cells osteogenic differentiation by predominance of autophagy over selective mitophagy. J Cell Mol Med. 2016;20:2384-2404 pubmed publisher
	Patel N, Garikapati K, Ramaiah M, Polavarapu K, Bhadra U, Bhadra M. miR-15a/miR-16 induces mitochondrial dependent apoptosis in breast cancer cells by suppressing oncogene BMI1. Life Sci. 2016;164:60-70 pubmed publisher
	Huang X, Huang R, Gou S, Wang Z, Liao Z, Wang H. Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action. Bioconjug Chem. 2016;27:2132-48 pubmed publisher
	Csak T, Dolganiuc A, Kodys K, Nath B, Petrasek J, Bala S, et al. Mitochondrial antiviral signaling protein defect links impaired antiviral response and liver injury in steatohepatitis in mice. Hepatology. 2011;53:1917-31 pubmed publisher
	Zall H, Weber A, Besch R, Zantl N, Hacker G. Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1. Mol Cancer. 2010;9:164 pubmed publisher
	Kamal A, Sreekanth K, Kumar P, Shankaraiah N, Balakishan G, Ramaiah M, et al. Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines. Eur J Med Chem. 2010;45:2173-81 pubmed publisher
	Chinta S, Rane A, Yadava N, Andersen J, Nicholls D, Polster B. Reactive oxygen species regulation by AIF- and complex I-depleted brain mitochondria. Free Radic Biol Med. 2009;46:939-47 pubmed
	Kumar P, Coltas I, Kumar B, Chepeha D, Bradford C, Polverini P. Bcl-2 protects endothelial cells against gamma-radiation via a Raf-MEK-ERK-survivin signaling pathway that is independent of cytochrome c release. Cancer Res. 2007;67:1193-202 pubmed
	De Chiara G, Marcocci M, Torcia M, Lucibello M, Rosini P, Bonini P, et al. Bcl-2 Phosphorylation by p38 MAPK: identification of target sites and biologic consequences. J Biol Chem. 2006;281:21353-61 pubmed
	Chen M, Gan H, Remold H. A mechanism of virulence: virulent Mycobacterium tuberculosis strain H37Rv, but not attenuated H37Ra, causes significant mitochondrial inner membrane disruption in macrophages leading to necrosis. J Immunol. 2006;176:3707-16 pubmed
	Meyer L, Karawajew L, Schrappe M, Ludwig W, Debatin K, Stahnke K. Cytochrome c-related caspase-3 activation determines treatment response and relapse in childhood precursor B-cell ALL. Blood. 2006;107:4524-31 pubmed
	Yamasaki E, Wada A, Kumatori A, Nakagawa I, Funao J, Nakayama M, et al. Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteins Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation. J Biol Chem. 2006;281:11250-9 pubmed
	Arnoult D, Grodet A, Lee Y, Estaquier J, Blackstone C. Release of OPA1 during apoptosis participates in the rapid and complete release of cytochrome c and subsequent mitochondrial fragmentation. J Biol Chem. 2005;280:35742-50 pubmed
	Boden G, Homko C, Mozzoli M, Showe L, Nichols C, Cheung P. Thiazolidinediones upregulate fatty acid uptake and oxidation in adipose tissue of diabetic patients. Diabetes. 2005;54:880-5 pubmed
	Liang Y, Eid M, Lewis R, Kumar M. Mitochondria from TRAIL-resistant prostate cancer cells are capable of responding to apoptotic stimuli. Cell Signal. 2005;17:243-51 pubmed
	Mohr A, Zwacka R, Debatin K, Stahnke K. A novel method for the combined flow cytometric analysis of cell cycle and cytochrome c release. Cell Death Differ. 2004;11:1153-4 pubmed
	Colell A, Garcia Ruiz C, Lluis J, Coll O, Mari M, Fernandez Checa J. Cholesterol impairs the adenine nucleotide translocator-mediated mitochondrial permeability transition through altered membrane fluidity. J Biol Chem. 2003;278:33928-35 pubmed
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	Chandra D, Liu J, Tang D. Early mitochondrial activation and cytochrome c up-regulation during apoptosis. J Biol Chem. 2002;277:50842-54 pubmed
	Bitzer M, Armeanu S, Prinz F, Ungerechts G, Wybranietz W, Spiegel M, et al. Caspase-8 and Apaf-1-independent caspase-9 activation in Sendai virus-infected cells. J Biol Chem. 2002;277:29817-24 pubmed
	Takuma K, Phuagphong P, Lee E, Mori K, Baba A, Matsuda T. Anti-apoptotic effect of cGMP in cultured astrocytes: inhibition by cGMP-dependent protein kinase of mitochondrial permeable transition pore. J Biol Chem. 2001;276:48093-9 pubmed
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	Wang G, Klein J, Kang Y. Metallothionein inhibits doxorubicin-induced mitochondrial cytochrome c release and caspase-3 activation in cardiomyocytes. J Pharmacol Exp Ther. 2001;298:461-8 pubmed
	Shen H, Yang C, Ding W, Liu J, Ong C. Superoxide radical-initiated apoptotic signalling pathway in selenite-treated HepG(2) cells: mitochondria serve as the main target. Free Radic Biol Med. 2001;30:9-21 pubmed
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	Datta R, Oki E, Endo K, Biedermann V, Ren J, Kufe D. XIAP regulates DNA damage-induced apoptosis downstream of caspase-9 cleavage. J Biol Chem. 2000;275:31733-8 pubmed
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	Walczak H, Bouchon A, Stahl H, Krammer P. Tumor necrosis factor-related apoptosis-inducing ligand retains its apoptosis-inducing capacity on Bcl-2- or Bcl-xL-overexpressing chemotherapy-resistant tumor cells. Cancer Res. 2000;60:3051-7 pubmed
	Jemmerson R, Johnson J, Burrell E, Taylor P, Jenkins M. A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells. Eur J Immunol. 1991;21:143-51 pubmed

product information

master code :

NB100-56503

SKU :

NB100-56503

product name :

Cytochrome c Antibody (7H8.2C12) - BSA Free

unit size :

0.1 mg (also 0.025 mg)

description :

The Cytochrome c Antibody (7H8.2C12) - BSA Free from Novus is a mouse monoclonal antibody to Cytochrome c. This antibody reacts with drosophila,human,insect - drosophila,mouse,rabbit,rat. The Cytochrome c Antibody (7H8.2C12) - BSA Free has been validated for the following applications: Flow Cytometry Control,Flow Cytometry,Western Blot,Flow (Cell Surface),Immunocytochemistry/ Immunofluorescence,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunoblotting,Flow (Intracellular),Simple Western.

target :

Cytochrome c

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

7H8.2C12

concentration :

1.0 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).

isotype :

IgG2b Kappa

purity :

Protein G purified

species :

Drosophila,Human,Insect - Drosophila,Mouse,Rabbit,Rat

specificity :

This antibody recognizes total cytochrome C which includes both apocytochrome (i

gene symbol :

CYCS

Antibody validation :

Biological Validation

accessionNumbers :

P99999

applications :

Flow Cytometry Control,Flow Cytometry,Western Blot,Flow (Cell Surface),Immunocytochemistry/ Immunofluorescence,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunoblotting,Flow (Intracellular),Simple Western

USD :

459 USD

alt names :

CYCHCS, cytochrome c, cytochrome c, somatic, THC4

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage