BID Antibody | Novus Biologicals NB100-56108 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

BID Antibody

catalog :

NB100-56108

quantity :

0.05 ml (also 0.025 ml)

price :

229 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

8F7

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 8

Reference
Turner N, Lord C, Iorns E, Brough R, Swift S, Elliott R, et al. A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitor. EMBO J. 2008;27:1368-77 pubmed publisher
Lee M, Tseng R, Hsu H, Chen J, Tzao C, Ho W, et al. Epigenetic inactivation of the chromosomal stability control genes BRCA1, BRCA2, and XRCC5 in non-small cell lung cancer. Clin Cancer Res. 2007;13:832-8 pubmed
Al Mulla F, Abdulrahman M, Varadharaj G, Akhter N, Anim J. BRCA1 gene expression in breast cancer: a correlative study between real-time RT-PCR and immunohistochemistry. J Histochem Cytochem. 2005;53:621-9 pubmed
Bernard Gallon D, Dechelotte P, Le Corre L, Chalabi N, Vissac Sabatier C, Bignon Y. Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia. Anticancer Res. 2004;24:321-4 pubmed
Ongusaha P, Ouchi T, Kim K, Nytko E, Kwak J, Duda R, et al. BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest. Oncogene. 2003;22:3749-58 pubmed
Reed W, Sandstad B, Holm R, Nesland J. The prognostic impact of hormone receptors and c-erbB-2 in pregnancy-associated breast cancer and their correlation with BRCA1 and cell cycle modulators. Int J Surg Pathol. 2003;11:65-74 pubmed
Okada S, Ouchi T. Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization. J Biol Chem. 2003;278:2015-20 pubmed
Bernard Gallon D, Dechelotte P, Vissac C, Aunoble B, Cravello L, Malpuech G, et al. BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite. Breast Cancer Res. 2001;3:61-5 pubmed

product information

brand :

Novus

master code :

NB100-56108

SKU :

NB100-56108

product name :

BID Antibody

description :

The BID Antibody from Novus is a rabbit polyclonal antibody to BID. This antibody reacts with human, mouse, rat, canine. The BID Antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin.

target :

BID

category :

Primary Antibodies

unit size :

0.05 ml (also 0.025 ml)

buffer :

Whole antisera

clonality :

Polyclonal

conjugate :

Unconjugated

host :

Rabbit

immunogen :

A synthetic peptide corresponding to amino acids 28-50 (CSDNSFRRELDALGHELPVLAPQ) of human Bid (also known as Bid, isoform 2) was used as immunogen; GenBank no. NP_001187.1. Human Bid is a 195 amino acid protein.

isotype :

IgG

purity :

Unpurified

species :

Human, Mouse, Rat, Canine

specificity :

Full-length Bid is known to undergo cleavage/truncation (reviewed in Yin, 2006). Bid was initially found to be cleaved and activated by capspase-8 following death receptor activation. The term "Bid" was first used to described the caspase-8 cleaved/truncated C-terminal Bid. Bid can be also cleaved by other proteases such as Granzyme B, calpains and cathepsin. The C-terminal portion is considered to be the active Bid moiety. This active form can translocate from the cytosol to the mitochondria. Therefore, the appearance of Bid in the mitochondria is considered to be an indication of active Bid. However, it should also be noted that Bid has been shown to translocate to the mitochondria without cleavage in some model systems. The proteolytic cleavage of Bid usually occurs in the unstructured loop region between the alpha 2 and alpha 3 helices, which is between amino acids (aa) 41 and 79 of Bid. For example, the caspase-8/3 cleavage site is at 60 (human) and 59 (mouse). It should be noted that after cleavage, the smaller N-terminal portion of Bid is not necessarily separated from the larger C-terminal portion. The exact size of the C-terminal cleavage products depends on the Bid cleavage sites; generally 11-15 kDa C-truncated cleavage forms are generated. Shorter C-terminal Bid cleavage products may result from protease cleavage that involves both caspases and other proteases. Full length Bid is quite stable, but cleaved/truncated forms may have a short half-life (less than 1.5 h) and degrade rapidly. Therefore, a time course may be useful when detecting Bid cleavage fragments.

gene symbol :

BID

catalog number base :

NB100-56108

applications :

Western Blot, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin

USD :

229 USD

alt names :

apoptic death agonist, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, desmocollin type 4, FP497, Human BID coding sequence, MGC15319, MGC42355, p22 BID, tbid

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.