This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Mouse Monoclonal TLR4 Antibody (HTA125) [FITC]
catalog :
NB100-56059
quantity :
0.1 ml
price :
369 USD
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
HTA125
reactivity :
human, mouse, dog
application :
western blot, immunocytochemistry, flow cytometry
citations: 21
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human
Mempel M, Voelcker V, Köllisch G, Plank C, Rad R, Gerhard M, et al. Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent. J Invest Dermatol. 2003;121:1389-96 pubmed
Harman A, Bye C, Nasr N, Sandgren K, Kim M, Mercier S, et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013;190:66-79 pubmed publisher
Matsunaga N, Tsuchimori N, Matsumoto T, Ii M. TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules. Mol Pharmacol. 2011;79:34-41 pubmed publisher
Pillay J, Ramakers B, Kamp V, Loi A, Lam S, Hietbrink F, et al. Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia. J Leukoc Biol. 2010;88:211-20 pubmed publisher
Wu C, Chi P, Hsieh H, Luo S, Yang C. TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2. J Cell Physiol. 2010;223:480-91 pubmed publisher
Pietschmann K, Beetz S, Welte S, Martens I, Gruen J, Oberg H, et al. Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes. Scand J Immunol. 2009;70:245-55 pubmed publisher
O Hara S, Small A, Gajdos G, Badley A, Chen X, Larusso N. HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum. J Infect Dis. 2009;199:1195-204 pubmed publisher
Yang X, Fullerton D, Su X, Ao L, Cleveland J, Meng X. Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2. J Am Coll Cardiol. 2009;53:491-500 pubmed publisher
Hammadi A, Billard C, Faussat A, Kolb J. Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation. Nitric Oxide. 2008;19:138-45 pubmed publisher
Burgener I, Jungi T. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008;124:184-91 pubmed publisher
Prabha C, Rajashree P, Sulochana D. TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid. Immunol Lett. 2008;117:26-34 pubmed publisher
Pegu A, Qin S, Fallert Junecko B, Nisato R, Pepper M, Reinhart T. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008;180:3399-405 pubmed
Murciano C, Villamon E, Yáñez A, Murciano J, Mir A, O Connor J, et al. In vitro response to Candida albicans in cultures of whole human blood from young and aged donors. FEMS Immunol Med Microbiol. 2007;51:327-35 pubmed
Wong C, Cheung P, Ip W, Lam C. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007;37:85-96 pubmed
Basu S, Pathak S, Banerjee A, Pathak S, Bhattacharyya A, Yang Z, et al. Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha. J Biol Chem. 2007;282:1039-50 pubmed
Shahrara S, Park C, Temkin V, Jarvis J, Volin M, Pope R. RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes. J Immunol. 2006;177:5077-87 pubmed
Zanoni G, Navone R, Lunardi C, Tridente G, Bason C, Sivori S, et al. In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of monocytes. PLoS Med. 2006;3:e358 pubmed
Xu S, Koldovsky U, Xu M, Wang D, Fitzpatrick E, Son G, et al. High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production. Surgery. 2006;140:170-8 pubmed
Scheel O, Papavlassopoulos M, Blunck R, Gebert A, Hartung T, Zahringer U, et al. Cell activation by ligands of the toll-like receptor and interleukin-1 receptor family depends on the function of the large-conductance potassium channel MaxiK in human macrophages. Infect Immun. 2006;74:4354-6 pubmed
Konno K, Wakabayashi Y, Akashi Takamura S, Ishii T, Kobayashi M, Takahashi K, et al. A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression. Biochem Biophys Res Commun. 2006;339:1076-82 pubmed
Cognasse F, Hamzeh H, Chavarin P, Acquart S, Genin C, Garraud O. Evidence of Toll-like receptor molecules on human platelets. Immunol Cell Biol. 2005;83:196-8 pubmed
product information
Catalog Number :
NB100-56059
Product Name :
Mouse Monoclonal TLR4 Antibody (HTA125) [FITC]
List Price :
369 USD
product type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, TOLL, toll-like receptor 4
Buffer :
50mM Sodium Borate
Clonality :
Monoclonal
Clone :
HTA125
Conjugate :
FITC
Application Summary :
Flow Cytometry, Western Blot (Negative)
Host :
Mouse
Immunogen :
This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.
Isotype :
IgG2a
Preservative :
0.05% Sodium Azide
Purity :
Protein G purified
CrossReactivity :
Canine, Guinea Pig, Human, Mouse, Porcine, Primate
Specificity :
The has been shown to block the activation of monocytes with LPS (Paik, et al, 2003).
Storage :
Store at 4C in the dark.
Swissprot Human :
O00206
Uses :
Confocal Microscopy: see Scheel et al. (2006) for details FA (Neutralization): please Basek et al, 2005 for details. Flow (Cell Surface): 2-5 ug/ 1x10^6 cells IF/ICC: please see Schneeman et al (2005) for details. IP: please see Shimazu et al, 1999 for details
Research Area :
Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction, Toll Like Receptors
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.