Mouse Monoclonal Cytochrome c Antibody (7H8.2C12) [Biotin] | Novus Biologicals NB100-55775 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Mouse Monoclonal Cytochrome c Antibody (7H8.2C12) [Biotin]

catalog :

NB100-55775

quantity :

0.1 ml

price :

369 USD

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

7H8.2C12

reactivity :

fruit flies, human, mouse, rat, dog

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

citations: 32

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse	Douglas R, Ryu J, Kanaan A, Del Carmen Rivero M, Dugan L, Haddad G, et al. Neuronal death during combined intermittent hypoxia/hypercapnia is due to mitochondrial dysfunction. Am J Physiol Cell Physiol. 2010;298:C1594-602 pubmed publisher
western blot; fruit flies	Wong S, Chen Y, Chan C, Chan C, Chan P, Chui Y, et al. In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila. Biochem Biophys Res Commun. 2005;337:720-9 pubmed
western blot; rat	Zhu C, Wang X, Cheng X, Qiu L, Xu F, Simbruner G, et al. Post-ischemic hypothermia-induced tissue protection and diminished apoptosis after neonatal cerebral hypoxia-ischemia. Brain Res. 2004;996:67-75 pubmed
western blot; rat	Colell A, Garcia Ruiz C, Lluis J, Coll O, Mari M, Fernandez Checa J. Cholesterol impairs the adenine nucleotide translocator-mediated mitochondrial permeability transition through altered membrane fluidity. J Biol Chem. 2003;278:33928-35 pubmed
western blot; rat	Takuma K, Phuagphong P, Lee E, Mori K, Baba A, Matsuda T. Anti-apoptotic effect of cGMP in cultured astrocytes: inhibition by cGMP-dependent protein kinase of mitochondrial permeable transition pore. J Biol Chem. 2001;276:48093-9 pubmed
western blot; rat	Neame S, Rubin L, Philpott K. Blocking cytochrome c activity within intact neurons inhibits apoptosis. J Cell Biol. 1998;142:1583-93 pubmed
	Csak T, Dolganiuc A, Kodys K, Nath B, Petrasek J, Bala S, et al. Mitochondrial antiviral signaling protein defect links impaired antiviral response and liver injury in steatohepatitis in mice. Hepatology. 2011;53:1917-31 pubmed publisher
	Zall H, Weber A, Besch R, Zantl N, Hacker G. Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1. Mol Cancer. 2010;9:164 pubmed publisher
	Kamal A, Sreekanth K, Kumar P, Shankaraiah N, Balakishan G, Ramaiah M, et al. Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines. Eur J Med Chem. 2010;45:2173-81 pubmed publisher
	Kumar P, Coltas I, Kumar B, Chepeha D, Bradford C, Polverini P. Bcl-2 protects endothelial cells against gamma-radiation via a Raf-MEK-ERK-survivin signaling pathway that is independent of cytochrome c release. Cancer Res. 2007;67:1193-202 pubmed
	De Chiara G, Marcocci M, Torcia M, Lucibello M, Rosini P, Bonini P, et al. Bcl-2 Phosphorylation by p38 MAPK: identification of target sites and biologic consequences. J Biol Chem. 2006;281:21353-61 pubmed
	Chen M, Gan H, Remold H. A mechanism of virulence: virulent Mycobacterium tuberculosis strain H37Rv, but not attenuated H37Ra, causes significant mitochondrial inner membrane disruption in macrophages leading to necrosis. J Immunol. 2006;176:3707-16 pubmed
	Meyer L, Karawajew L, Schrappe M, Ludwig W, Debatin K, Stahnke K. Cytochrome c-related caspase-3 activation determines treatment response and relapse in childhood precursor B-cell ALL. Blood. 2006;107:4524-31 pubmed
	Yamasaki E, Wada A, Kumatori A, Nakagawa I, Funao J, Nakayama M, et al. Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteins Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation. J Biol Chem. 2006;281:11250-9 pubmed
	Zhu C, Xu F, Wang X, Shibata M, Uchiyama Y, Blomgren K, et al. Different apoptotic mechanisms are activated in male and female brains after neonatal hypoxia-ischaemia. J Neurochem. 2006;96:1016-27 pubmed
	Arnoult D, Grodet A, Lee Y, Estaquier J, Blackstone C. Release of OPA1 during apoptosis participates in the rapid and complete release of cytochrome c and subsequent mitochondrial fragmentation. J Biol Chem. 2005;280:35742-50 pubmed
	Boden G, Homko C, Mozzoli M, Showe L, Nichols C, Cheung P. Thiazolidinediones upregulate fatty acid uptake and oxidation in adipose tissue of diabetic patients. Diabetes. 2005;54:880-5 pubmed
	Liang Y, Eid M, Lewis R, Kumar M. Mitochondria from TRAIL-resistant prostate cancer cells are capable of responding to apoptotic stimuli. Cell Signal. 2005;17:243-51 pubmed
	Liu C, Lee C, Hong C, Wei Y. Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis. Ann N Y Acad Sci. 2004;1011:133-45 pubmed
	Chandra D, Liu J, Tang D. Early mitochondrial activation and cytochrome c up-regulation during apoptosis. J Biol Chem. 2002;277:50842-54 pubmed
	Bitzer M, Armeanu S, Prinz F, Ungerechts G, Wybranietz W, Spiegel M, et al. Caspase-8 and Apaf-1-independent caspase-9 activation in Sendai virus-infected cells. J Biol Chem. 2002;277:29817-24 pubmed
	Junn E, Mouradian M. Apoptotic signaling in dopamine-induced cell death: the role of oxidative stress, p38 mitogen-activated protein kinase, cytochrome c and caspases. J Neurochem. 2001;78:374-83 pubmed
	Wang G, Klein J, Kang Y. Metallothionein inhibits doxorubicin-induced mitochondrial cytochrome c release and caspase-3 activation in cardiomyocytes. J Pharmacol Exp Ther. 2001;298:461-8 pubmed
	Shen H, Yang C, Ding W, Liu J, Ong C. Superoxide radical-initiated apoptotic signalling pathway in selenite-treated HepG(2) cells: mitochondria serve as the main target. Free Radic Biol Med. 2001;30:9-21 pubmed
	Narvaez C, Welsh J. Role of mitochondria and caspases in vitamin D-mediated apoptosis of MCF-7 breast cancer cells. J Biol Chem. 2001;276:9101-7 pubmed
	Datta R, Oki E, Endo K, Biedermann V, Ren J, Kufe D. XIAP regulates DNA damage-induced apoptosis downstream of caspase-9 cleavage. J Biol Chem. 2000;275:31733-8 pubmed
	Maccarrone M, Lorenzon T, Bari M, Melino G, Finazzi Agro A. Anandamide induces apoptosis in human cells via vanilloid receptors. Evidence for a protective role of cannabinoid receptors. J Biol Chem. 2000;275:31938-45 pubmed
	Maeno E, Ishizaki Y, Kanaseki T, Hazama A, Okada Y. Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis. Proc Natl Acad Sci U S A. 2000;97:9487-92 pubmed
	Walczak H, Bouchon A, Stahl H, Krammer P. Tumor necrosis factor-related apoptosis-inducing ligand retains its apoptosis-inducing capacity on Bcl-2- or Bcl-xL-overexpressing chemotherapy-resistant tumor cells. Cancer Res. 2000;60:3051-7 pubmed
	Pervaiz S, Seyed M, Hirpara J, Clement M, Loh K. Purified photoproducts of merocyanine 540 trigger cytochrome C release and caspase 8-dependent apoptosis in human leukemia and melanoma cells. Blood. 1999;93:4096-108 pubmed
	Fujimura M, Morita Fujimura Y, Murakami K, Kawase M, Chan P. Cytosolic redistribution of cytochrome c after transient focal cerebral ischemia in rats. J Cereb Blood Flow Metab. 1998;18:1239-47 pubmed
	Jemmerson R, Johnson J, Burrell E, Taylor P, Jenkins M. A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells. Eur J Immunol. 1991;21:143-51 pubmed

product information

Catalog Number :

NB100-55775

Product Name :

Mouse Monoclonal Cytochrome c Antibody (7H8.2C12) [Biotin]

List Price :

369 USD

product type :

Primary Antibodies

Package Size :

0.1 ml

AltNames :

CYCHCS, cytochrome c, cytochrome c, somatic, THC4

Buffer :

PBS

Clonality :

Monoclonal

Clone :

7H8.2C12

Conjugate :

Biotin

Application Summary :

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Host :

Mouse

Immunogen :

Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).

Isotype :

IgG2b Kappa

Marker :

Mitochondria Marker

Preservative :

0.05% Sodium Azide

Purity :

Protein G purified

CrossReactivity :

Canine, Drosophila, Equine, Human, Mammal, Mouse, Porcine, Rabbit, Rat

Specificity :

An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Storage :

Store at 4C. Do not freeze.

Swissprot Human :

P99999

Uses :

Useful in Flow Cytometry (Intracellular): See Mohr (2004) et al. Immunocytochemistry/Immunofluorescence: See Yamasaki (2006) et al. Immunohistochemistry-Paraffin: See Fujimara (1998). et al.

Research Area :

Apoptosis, Core ESC Like Genes, Lipid and Metabolism, Stem Cell Markers