product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Histone H2AX [p Ser139] Antibody - BSA Free
catalog :
NB100-384
quantity :
0.1 ml
price :
539 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
AF647
clone name :
5B12
reactivity :
human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 115
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 8c
Konopka A, Whelan D, Jamali M, Perri E, Shahheydari H, Toth R, et al. Impaired NHEJ repair in amyotrophic lateral sclerosis is associated with TDP-43 mutations. Mol Neurodegener. 2020;15:51 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 4d
Pan H, Jiang N, Sun S, Jiang H, Xu J, Jiang X, et al. UHRF1-repressed 5'-hydroxymethylcytosine is essential for the male meiotic prophase I. Cell Death Dis. 2020;11:142 pubmed publisher
  • western blot; mouse; 1:3000; loading ...; fig 3b
Quach C, Song Y, Guo H, Li S, Maazi H, Fung M, et al. A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice. Nat Commun. 2019;10:5681 pubmed publisher
  • immunocytochemistry; human; 1:2000; loading ...; fig 3a, 4e, 6b
  • western blot; human; loading ...; fig s3a
Pillay N, Tighe A, Nelson L, Littler S, Coulson Gilmer C, Bah N, et al. DNA Replication Vulnerabilities Render Ovarian Cancer Cells Sensitive to Poly(ADP-Ribose) Glycohydrolase Inhibitors. Cancer Cell. 2019;35:519-533.e8 pubmed publisher
  • immunocytochemistry; rat; 1:200; fig 1
Mata Garrido J, Casafont I, Tapia O, Berciano M, Lafarga M. Neuronal accumulation of unrepaired DNA in a novel specific chromatin domain: structural, molecular and transcriptional characterization. Acta Neuropathol Commun. 2016;4:41 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 4b
Liu X, Wang S, Guo X, Wei F, Yin J, Zang Y, et al. Exogenous p53 and ASPP2 expression enhances rAdV-TK/ GCV-induced death in hepatocellular carcinoma cells lacking functional p53. Oncotarget. 2016;7:18896-905 pubmed publisher
  • immunocytochemistry; human; 1:300; fig 2
Francia S, Cabrini M, Matti V, Oldani A, d Adda di Fagagna F. DICER, DROSHA and DNA damage response RNAs are necessary for the secondary recruitment of DNA damage response factors. J Cell Sci. 2016;129:1468-76 pubmed publisher
  • immunohistochemistry; mouse; 1:2000; loading ...; fig 3
Phaeton R, Jiang Z, Revskaya E, Fisher D, Goldberg G, Dadachova E. Beta emitters rhenium-188 and lutetium-177 are equally effective in radioimmunotherapy of HPV-positive experimental cervical cancer. Cancer Med. 2016;5:9-16 pubmed publisher
  • western blot; human; fig 7
Cristini A, Park J, Capranico G, Legube G, Favre G, Sordet O. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res. 2016;44:1161-78 pubmed publisher
Chen J, Merrick K, Kong Y, Izrael Tomasevic A, Eng G, Handly E, et al. An RNA damage response network mediates the lethality of 5-FU in colorectal cancer. Cell Rep Med. 2024;5:101778 pubmed publisher
Audet Delage Y, St Louis C, Minarrieta L, McGuirk S, Kurreal I, Annis M, et al. Spatiotemporal modeling of chemoresistance evolution in breast tumors uncovers dependencies on SLC38A7 and SLC46A1. Cell Rep. 2023;42:113191 pubmed publisher
Wu D, Huang H, Chen T, Gai X, Li Q, Wang C, et al. The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination. Cell Discov. 2023;9:99 pubmed publisher
Nonaka K, Takubo K, Aida J, Watai Y, Komatsu A, Gomi F, et al. Accelerated telomere shortening in adrenal zona reticularis in patients with prolonged critical illness. Front Endocrinol (Lausanne). 2023;14:1244553 pubmed publisher
McCann J, Cristini A, Law E, Lee S, Tellier M, Carpenter M, et al. APOBEC3B regulates R-loops and promotes transcription-associated mutagenesis in cancer. Nat Genet. 2023;55:1721-1734 pubmed publisher
Ershova E, Savinova E, Kameneva L, Porokhovnik L, Veiko R, Salimova T, et al. Satellite III (1q12) Copy Number Variation in Cultured Human Skin Fibroblasts from Schizophrenic Patients and Healthy Controls. Front Biosci (Landmark Ed). 2023;28:191 pubmed publisher
Ershova E, Savinova E, Kameneva L, Porokhovnik L, Veiko R, Salimova T, et al. Antipsychotics Affect Satellite III (1q12) Copy Number Variations in the Cultured Human Skin Fibroblasts. Int J Mol Sci. 2023;24: pubmed publisher
Sun X, Gao C, Xu X, Li M, Zhao X, Wang Y, et al. FBL promotes cancer cell resistance to DNA damage and BRCA1 transcription via YBX1. EMBO Rep. 2023;24:e56230 pubmed publisher
Zhang Z, Shayani G, Xu Y, Kim A, Hong Y, Feng H, et al. Induction of Senescence by Loss of Gata4 in Cardiac Fibroblasts. Cells. 2023;12: pubmed publisher
Huang Y, Hertzel A, Fish S, Halley C, Bohm E, Martinez H, et al. TP53/p53 Facilitates Stress-Induced Exosome and Protein Secretion by Adipocytes. Diabetes. 2023;72:1560-1573 pubmed publisher
So H, Kim H, Lee J, You C, Yun C, Jeong H, et al. Protein Arginine Methyltransferase 1 Ablation in Motor Neurons Causes Mitochondrial Dysfunction Leading to Age-related Motor Neuron Degeneration with Muscle Loss. Research (Wash D C). 2023;6:0158 pubmed publisher
Corbett C, St Paul A, Leigh T, Kelemen S, Peluzzo A, Okune R, et al. Genetic Deletion of FXR1 Reduces Intimal Hyperplasia and Induces Senescence in Vascular Smooth Muscle Cells. Am J Pathol. 2023;193:638-653 pubmed publisher
He D, Tao L, Cai B, Chen X, Wang Y, Li S, et al. eIF2α incites photoreceptor cell and retina damage by all-trans-retinal. J Biol Chem. 2023;299:104686 pubmed publisher
Nakamichi R, Hishikawa A, Chikuma S, Yoshimura A, Sasaki T, Hashiguchi A, et al. DNA-damaged podocyte-CD8 T cell crosstalk exacerbates kidney injury by altering DNA methylation. Cell Rep. 2023;42:112302 pubmed publisher
Tamaki Y, Ross J, Alipour P, Castonguay C, Li B, Catoire H, et al. Spinal cord extracts of amyotrophic lateral sclerosis spread TDP-43 pathology in cerebral organoids. PLoS Genet. 2023;19:e1010606 pubmed publisher
Zeng F, Parker K, Zhan Y, Miller M, Zhu M. Upregulated DNA Damage-Linked Biomarkers in Parkinson's Disease Model Mice. ASN Neuro. 2023;15:17590914231152099 pubmed publisher
Yoshimoto N, Hayashi K, Hishikawa A, Hashiguchi A, Nakamichi R, Sugita Nishimura E, et al. Significance of podocyte DNA damage and glomerular DNA methylation in CKD patients with proteinuria. Hypertens Res. 2023;46:1000-1008 pubmed publisher
Pai G, Roohollahi K, Rockx D, de Jong Y, Stoepker C, Pennings C, et al. Genome-wide siRNA screens identify RBBP9 function as a potential target in Fanconi anaemia-deficient head-and-neck squamous cell carcinoma. Commun Biol. 2023;6:37 pubmed publisher
Kostyuk S, Ershova E, Martynov A, Artyushin A, Porokhovnik L, Malinovskaya E, et al. In Vitro Analysis of Biological Activity of Circulating Cell-Free DNA Isolated from Blood Plasma of Schizophrenic Patients and Healthy Controls-Part 2: Adaptive Response. Genes (Basel). 2022;13: pubmed publisher
Lawson C, Peel S, Jayo A, Corrigan A, Iyer P, Baxter Dalrymple M, et al. Nuclear fascin regulates cancer cell survival. elife. 2022;11: pubmed publisher
Karlowitz R, Stanifer M, Roedig J, Andrieux G, Bojkova D, Bechtel M, et al. USP22 controls type III interferon signaling and SARS-CoV-2 infection through activation of STING. Cell Death Dis. 2022;13:684 pubmed publisher
Lin W, Chen J, Wen X, He W, Zarceno G, Chen Y, et al. DDX18 prevents R-loop-induced DNA damage and genome instability via PARP-1. Cell Rep. 2022;40:111089 pubmed publisher
Molina A, Bonnet F, Pignolet J, Lobjois V, Bel Vialar S, Gautrais J, et al. Single-cell imaging of the cell cycle reveals CDC25B-induced heterogeneity of G1 phase length in neural progenitor cells. Development. 2022;149: pubmed publisher
Fielder E, Wan T, Alimohammadiha G, Ishaq A, Low E, Weigand B, et al. Short senolytic or senostatic interventions rescue progression of radiation-induced frailty and premature ageing in mice. elife. 2022;11: pubmed publisher
Lasagni Vitar R, Triani F, Barbariga M, Fonteyne P, Rama P, Ferrari G. Substance P/neurokinin-1 receptor pathway blockade ameliorates limbal stem cell deficiency by modulating mTOR pathway and preventing cell senescence. Stem Cell Reports. 2022;17:849-863 pubmed publisher
Xu J, Gao J, Liu J, Huang X, Zhang H, Ma A, et al. ZFP541 maintains the repression of pre-pachytene transcriptional programs and promotes male meiosis progression. Cell Rep. 2022;38:110540 pubmed publisher
Sain Basu D, Bhavsar R, Gulami I, Chavda S, Lingamallu S, Muddashetty R, et al. FMRP protects the lung from xenobiotic stress by facilitating the integrated stress response. J Cell Sci. 2022;135: pubmed publisher
xc7 oku J, Booth D, Skoda J, Pedrotty M, Vogel J, Liu K, et al. Reduced ER-mitochondria connectivity promotes neuroblastoma multidrug resistance. EMBO J. 2022;41:e108272 pubmed publisher
Steiner S, Seleznik G, Reding T, Stopic M, Lenggenhager D, Ten Buren E, et al. De novo expression of gastrokines in pancreatic precursor lesions impede the development of pancreatic cancer. Oncogene. 2022;41:1507-1517 pubmed publisher
Yang Y, Lu H, Chen C, Lyu Y, Cole R, Semenza G. HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia. Nat Commun. 2022;13:316 pubmed publisher
Samivel R, Subramanian U, Ali Khan A, Kirat O, Masmali A, Almubrad T, et al. Lipopolysaccharide Enhances Genotoxicity by Activating GADD45G and NF-κB in Human Corneal Epithelial Cells. Oxid Med Cell Longev. 2022;2022:4328116 pubmed publisher
Ma H, Li T, Xie X, Jiang L, Ye J, Gong C, et al. RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes. Sci Adv. 2022;8:eabk1789 pubmed publisher
Herrera F, Ronet C, Ochoa de Olza M, Barras D, Crespo I, Andreatta M, et al. Low-Dose Radiotherapy Reverses Tumor Immune Desertification and Resistance to Immunotherapy. Cancer Discov. 2022;12:108-133 pubmed publisher
Hishikawa A, Hayashi K, Kubo A, Miyashita K, Hashiguchi A, Kinouchi K, et al. DNA repair factor KAT5 prevents ischemic acute kidney injury through glomerular filtration regulation. iScience. 2021;24:103436 pubmed publisher
Min S, Lee H, Ji J, Heo Y, Kim Y, Chae S, et al. The chromatin remodeler RSF1 coordinates epigenetic marks for transcriptional repression and DSB repair. Nucleic Acids Res. 2021;49:12268-12283 pubmed publisher
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Sakama S, Kurusu K, Morita M, Oizumi T, Masugata S, Oka S, et al. An Enriched Environment Alters DNA Repair and Inflammatory Responses After Radiation Exposure. Front Immunol. 2021;12:760322 pubmed publisher
Zuo W, Chen G, Gao Z, Li S, Chen Y, Huang C, et al. Stage-resolved Hi-C analyses reveal meiotic chromosome organizational features influencing homolog alignment. Nat Commun. 2021;12:5827 pubmed publisher
Victor J, Deutsch J, Whitaker A, Lamkin E, March A, Zhou P, et al. SARS-CoV-2 triggers DNA damage response in Vero E6 cells. Biochem Biophys Res Commun. 2021;579:141-145 pubmed publisher
Kim H, Kim T, Kim Y, Seong C, Cho J, Kim W, et al. Antioxidant and Antiproliferative Activity of Finasteride against Glioblastoma Cells. Pharmaceutics. 2021;13: pubmed publisher
Herok M, Wawrzynow B, Maluszek M, Olszewski M, Zylicz A, Zylicz M. Chemotherapy of HER2- and MDM2-Enriched Breast Cancer Subtypes Induces Homologous Recombination DNA Repair and Chemoresistance. Cancers (Basel). 2021;13: pubmed publisher
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Paramos de Carvalho D, Martins I, Cristóvão A, Dias A, Neves Silva D, Pereira T, et al. Targeting senescent cells improves functional recovery after spinal cord injury. Cell Rep. 2021;36:109334 pubmed publisher
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Fütterer A, Talavera Gutiérrez A, Pons T, de Celis J, Gutierrez J, Domínguez Plaza V, et al. Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels. Cell Death Dis. 2021;12:637 pubmed publisher
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Yousefzadeh M, Flores R, Zhu Y, Schmiechen Z, Brooks R, Trussoni C, et al. An aged immune system drives senescence and ageing of solid organs. Nature. 2021;594:100-105 pubmed publisher
van Vliet T, Varela Eirin M, Wang B, Borghesan M, Brandenburg S, Franzin R, et al. Physiological hypoxia restrains the senescence-associated secretory phenotype via AMPK-mediated mTOR suppression. Mol Cell. 2021;81:2041-2052.e6 pubmed publisher
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Li X, Wu L, Zopp M, Kopelov S, Du W. p53-TP53-Induced Glycolysis Regulator Mediated Glycolytic Suppression Attenuates DNA Damage and Genomic Instability in Fanconi Anemia Hematopoietic Stem Cells. Stem Cells. 2019;37:937-947 pubmed publisher
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Kim J, Sturgill D, Tran A, Sinclair D, Oberdoerffer P. Controlled DNA double-strand break induction in mice reveals post-damage transcriptome stability. Nucleic Acids Res. 2016;44:e64 pubmed publisher
Markova E, Somsedíková A, Vasilyev S, Pobijaková M, Lacková A, Lukačko P, et al. DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy. Int J Radiat Biol. 2015;91:934-45 pubmed publisher
Campbell A, Krupp B, Bushman J, Noble M, Pröschel C, Mayer Pröschel M. A novel mouse model for ataxia-telangiectasia with a N-terminal mutation displays a behavioral defect and a low incidence of lymphoma but no increased oxidative burden. Hum Mol Genet. 2015;24:6331-49 pubmed publisher
Durdik M, Košík P, Gursky J, Vokálová L, Markova E, Belyaev I. Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and γH2AX foci in human lymphocytes. Cytometry A. 2015;87:1070-8 pubmed publisher
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product information
master code :
NB100-384
SKU :
NB100-384
product name :
Histone H2AX [p Ser139] Antibody - BSA Free
unit size :
0.1 ml
description :
The Histone H2AX [p Ser139] Antibody - BSA Free from Novus is a rabbit polyclonal antibody to Histone H2AX. This antibody reacts with canine,human,insect - drosophila melanogaster,mouse,rat. The Histone H2AX [p Ser139] Antibody - BSA Free has been validated for the following applications: Immunohistochemistry,Western Blot,Immunocytochemistry/ Immunofluorescence,Chemotaxis,IF/IHC,Simple Western,Flow Cytometry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Chromatin Immunoprecipitation (ChIP),Knockout Validated.
target :
Histone H2AX
category :
Primary Antibodies
buffer :
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
clonality :
Polyclonal
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Rabbit
immunogen :
This Histone H2AX [p Ser139] Antibody was developed against to a region surrounding phosphorylated serine 139 of human histone H2AX [Swiss-Prot entry P16104] (GeneID 3014).
isotype :
IgG
purity :
Immunogen affinity purified
species :
Canine,Human,Insect - Drosophila melanogaster,Mouse,Rat
specificity :
The epitope maps to a region surrounding phosphorylated serine 139 of human histone H2AX
theoretical molecular weight :
15 kDa
gene symbol :
H2AX
Antibody validation :
Biological Validation
accessionNumbers :
P16104
applications :
Immunohistochemistry,Western Blot,Immunocytochemistry/ Immunofluorescence,Chemotaxis,IF/IHC,Simple Western,Flow Cytometry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Chromatin Immunoprecipitation (ChIP),Knockout Validated
USD :
539 USD
alt names :
H2A.X, H2A/X, H2AFX
storage :
Store at 4C. Do not freeze.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.