Rabbit Polyclonal ATP7b Antibody [HRP] | Novus Biologicals NB100-360H product information

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product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Rabbit Polyclonal ATP7b Antibody [HRP]

catalog :

NB100-360H

quantity :

0.1 ml

price :

399 USD

clonality :

polyclonal

host :

rabbit

conjugate :

HRP

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

product information

Catalog Number :

NB100-360H

Product Name :

Rabbit Polyclonal ATP7b Antibody [HRP]

List Price :

399 USD

product type :

Primary Antibodies

Package Size :

0.1 ml

AltNames :

ATPase, Cu(2+)- transporting, beta polypeptide, ATPase, Cu++ transporting, beta polypeptide, Copper pump 2, copper-transporting ATPase 2, EC 3.6.3, EC 3.6.3.4, PWD, WC1, WD, Wilson disease-associated protein, WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)

Buffer :

PBS

Clonality :

Polyclonal

Conjugate :

HRP

Application Summary :

Immunocytochemistry/Immunofluorescence, Western Blot

Host :

Rabbit

Immunogen :

A synthetic peptide made to an internal sequence near the N-terminus of human ATP7b. [UniProt# P35670]

Localization :

Golgi apparatus; Predominantly found in trans-Golgi network membrane; multi-pass membrane protein (By similarity). Isoform 2: Cytoplasm. The cleaved form WND/140 kDa is located in mitochondria.

Preservative :

No Preservative

Purity :

Immunogen affinity purified

CrossReactivity :

Human, Mouse, Rat

Storage :

Store at 4C in the dark.

Swissprot Human :

P35670

TMW :

165

Uses :

This ATP7b antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. By WB, this antibody recognizes a band at 165 kDa, representing ATP7b and also recognizes a faint non-specific band at ~195 kDa. In IHC-P, cytoplasmic and golgi staining was observed in mouse liver tissue (1:100 dilution) and human ovarian carcinoma cells (1:500 dilution). However, please note we also tested this antibody with IHC-P in mouse intestines and kidney and observed unexpected strong nuclear signal with lighter cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Research Area :

Cancer, Signal Transduction