product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
RPE65 Antibody (401.8B11.3D9) - BSA Free
catalog :
NB100-355
quantity :
0.2 ml (also 0.05 ml)
price :
469 USD
clonality :
monoclonal
host :
mouse
conjugate :
DyLight 594
clone name :
401.8B11.3D9
reactivity :
human, mouse, rat, dogs, chicken, bovine, pigs
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 101
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; rat; loading ...; fig 6b
Salas A, Duarri A, Fontrodona L, Ram xed rez D, Badia A, Isla Magran xe9 H, et al. Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration. Mol Ther Methods Clin Dev. 2021;20:688-702 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s8
Wright C, Uehara H, Kim Y, Yasuma T, Yasuma R, Hirahara S, et al. Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice. Proc Natl Acad Sci U S A. 2020;117:2579-2587 pubmed publisher
  • immunohistochemistry; human; 1:200; fig 4
Geng Z, Walsh P, Truong V, Hill C, Ebeling M, Kapphahn R, et al. Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS ONE. 2017;12:e0173575 pubmed publisher
  • immunocytochemistry; pigs ; 1:50; fig 3
Di Lauro S, Rodriguez Crespo D, Gayoso M, Garcia Gutierrez M, Pastor J, Srivastava G, et al. A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells. Mol Vis. 2016;22:243-53 pubmed
  • immunohistochemistry - frozen section; mouse
Yan R, He L, Zhan W, Wang S. Induction of ectopic retina-like tissue by transgenic expression of neurogenin. PLoS ONE. 2015;10:e0116171 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
  • immunohistochemistry; mouse; 1:100
Prasad T, Verma A, Li Q. Expression and cellular localization of the Mas receptor in the adult and developing mouse retina. Mol Vis. 2014;20:1443-55 pubmed
  • immunohistochemistry - frozen section; chicken
  • immunocytochemistry; chicken
Steinfeld J, Steinfeld I, Coronato N, Hampel M, Layer P, Araki M, et al. RPE specification in the chick is mediated by surface ectoderm-derived BMP and Wnt signalling. Development. 2013;140:4959-69 pubmed publisher
  • immunocytochemistry; mouse
Inoue Y, Hasegawa S, Yamada T, Date Y, Mizutani H, Nakata S, et al. Lignin Induces ES Cells to Differentiate into Neuroectodermal Cells through Mediation of the Wnt Signaling Pathway. PLoS ONE. 2013;8:e66376 pubmed publisher
  • immunocytochemistry; human
Singh A, Srivastava G, Martin L, Alonso M, Pastor J. Bioactive substrates for human retinal pigment epithelial cell growth from elastin-like recombinamers. J Biomed Mater Res A. 2014;102:639-46 pubmed publisher
  • western blot; pigs
Shirasawa M, Sonoda S, Terasaki H, Arimura N, Otsuka H, Yamashita T, et al. TNF-? disrupts morphologic and functional barrier properties of polarized retinal pigment epithelium. Exp Eye Res. 2013;110:59-69 pubmed publisher
  • immunocytochemistry; pigs
  • immunohistochemistry; pigs
Fernandez Bueno I, Rodriguez de la Rua E, Hileeto D, Parrado M, Regueiro Purriños M, Sala Puigdollers A, et al. Histology and immunochemistry evaluation of autologous translocation of retinal pigment epithelium-choroid graft in porcine eyes. Acta Ophthalmol. 2013;91:e125-32 pubmed publisher
  • immunocytochemistry; human
He Y, Ge J, Burke J, Myers R, Dong Z, Tombran Tink J. Mitochondria impairment correlates with increased sensitivity of aging RPE cells to oxidative stress. J Ocul Biol Dis Infor. 2010;3:92-108 pubmed publisher
  • immunocytochemistry; mouse
Jiang G, Sun D, Kaplan H, Shao H. Retinal astrocytes pretreated with NOD2 and TLR2 ligands activate uveitogenic T cells. PLoS ONE. 2012;7:e40510 pubmed publisher
  • immunocytochemistry; human
Cho M, Kim S, Ku S, Park J, Lee H, Yoo D, et al. Generation of retinal pigment epithelial cells from human embryonic stem cell-derived spherical neural masses. Stem Cell Res. 2012;9:101-9 pubmed publisher
  • immunohistochemistry - frozen section; mouse
  • immunohistochemistry; mouse
Xia H, Krebs M, Kaushal S, Scott E. Enhanced retinal pigment epithelium regeneration after injury in MRL/MpJ mice. Exp Eye Res. 2011;93:862-72 pubmed publisher
  • immunohistochemistry; mouse
Kleinman M, Kaneko H, Cho W, Dridi S, Fowler B, Blandford A, et al. Short-interfering RNAs induce retinal degeneration via TLR3 and IRF3. Mol Ther. 2012;20:101-8 pubmed publisher
  • flow cytometry; human
  • immunocytochemistry; human
  • immunohistochemistry; human
  • flow cytometry; mouse
  • immunocytochemistry; mouse
  • immunohistochemistry; mouse
Leung K, Gvritishvili A, Liu Y, Tombran Tink J. ZIP2 and ZIP4 mediate age-related zinc fluxes across the retinal pigment epithelium. J Mol Neurosci. 2012;46:122-37 pubmed publisher
  • immunocytochemistry; human
Harness J, Turovets N, Seiler M, Nistor G, Altun G, Agapova L, et al. Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells. PLoS ONE. 2011;6:e14499 pubmed publisher
  • immunocytochemistry; mouse
Ke Y, Jiang G, Sun D, Kaplan H, Shao H. Retinal Astrocytes respond to IL-17 differently than Retinal Pigment Epithelial cells. J Leukoc Biol. 2009;86:1377-84 pubmed publisher
  • immunohistochemistry - frozen section; dogs
  • immunohistochemistry; dogs
Gu D, Beltran W, Pearce Kelling S, Li Z, Acland G, Aguirre G. Steroids do not prevent photoreceptor degeneration in the light-exposed T4R rhodopsin mutant dog retina irrespective of AP-1 inhibition. Invest Ophthalmol Vis Sci. 2009;50:3482-94 pubmed publisher
  • immunohistochemistry - frozen section; mouse
  • immunohistochemistry; mouse
Harris J, Fisher R, Jorgensen M, Kaushal S, Scott E. CD133 progenitor cells from the bone marrow contribute to retinal pigment epithelium repair. Stem Cells. 2009;27:457-66 pubmed publisher
  • immunocytochemistry; human
Thumann G, Viethen A, Gaebler A, Walter P, Kaempf S, Johnen S, et al. The in vitro and in vivo behaviour of retinal pigment epithelial cells cultured on ultrathin collagen membranes. Biomaterials. 2009;30:287-94 pubmed publisher
  • immunocytochemistry; rat
Vossmerbaeumer U, Kuehl S, Kern S, Kluter H, Jonas J, Bieback K. Induction of retinal pigment epithelium properties in ciliary margin progenitor cells. Clin Exp Ophthalmol. 2008;36:358-66 pubmed publisher
  • immunohistochemistry - paraffin section; pigs
Kaempf S, Walter P, Salz A, Thumann G. Novel organotypic culture model of adult mammalian neurosensory retina in co-culture with retinal pigment epithelium. J Neurosci Methods. 2008;173:47-58 pubmed publisher
  • western blot; mouse
Chen M, Muckersie E, Robertson M, Fraczek M, Forrester J, Xu H. Characterization of a spontaneous mouse retinal pigment epithelial cell line B6-RPE07. Invest Ophthalmol Vis Sci. 2008;49:3699-706 pubmed publisher
  • immunohistochemistry - paraffin section; dogs
  • immunohistochemistry; dogs
Lall M, Ferrell J, Nagar S, Fleisher L, McGahan M. Iron regulates L-cystine uptake and glutathione levels in lens epithelial and retinal pigment epithelial cells by its effect on cytosolic aconitase. Invest Ophthalmol Vis Sci. 2008;49:310-9 pubmed publisher
  • immunocytochemistry; mouse
Jiang G, Ke Y, Sun D, Han G, Kaplan H, Shao H. Reactivation of uveitogenic T cells by retinal astrocytes derived from experimental autoimmune uveitis-prone B10RIII mice. Invest Ophthalmol Vis Sci. 2008;49:282-9 pubmed publisher
  • western blot; human
Jacobson S, Aleman T, Cideciyan A, Heon E, Golczak M, Beltran W, et al. Human cone photoreceptor dependence on RPE65 isomerase. Proc Natl Acad Sci U S A. 2007;104:15123-8 pubmed
Luo M, Almeida D, Dallacasagrande V, Hedhli N, Gupta M, D Amico D, et al. Annexin A2 promotes proliferative vitreoretinopathy in response to a macrophage inflammatory signal in mice. Nat Commun. 2024;15:8757 pubmed publisher
Shechter Y, Cohen R, Namestnikov M, Shapira A, Barak A, Barzelay A, et al. Sequential Fabrication of a Three-Layer Retina-like Structure. Gels. 2024;10: pubmed publisher
Hernandez B, Skiba N, Pl xf6 ssl K, Strain M, Liu Y, Grigsby D, et al. Polarized Desmosome and Hemidesmosome Shedding via Small Extracellular Vesicles is an Early Indicator of Outer Blood-Retina Barrier Dysfunction. J Extracell Biol. 2023;2: pubmed publisher
Souverein E, Nagiel A, Gnedeva K. Obtaining High-Quality Cryosections of Whole Rabbit Eye. J Vis Exp. 2023;: pubmed publisher
Hernandez B, Skiba N, Pl xf6 xdf l K, Strain M, Grigsby D, Kelly U, et al. Polarized Desmosome and Hemidesmosome Shedding via Exosomes is an Early Indicator of Outer Blood-Retina Barrier Dysfunction. bioRxiv. 2023;: pubmed publisher
Chang Y, Jenny L, Li Y, Cui X, Kong Y, Li Y, et al. CRISPR editing demonstrates rs10490924 raised oxidative stress in iPSC-derived retinal cells from patients with ARMS2/HTRA1-related AMD. Proc Natl Acad Sci U S A. 2023;120:e2215005120 pubmed publisher
Gonz xe1 lez Zamora J, Hernandez M, Recalde S, Bezunartea J, Montoliu A, Bilbao Malav xe9 V, et al. Matrix Metalloproteinase 13 Is Associated with Age-Related Choroidal Neovascularization. Antioxidants (Basel). 2023;12: pubmed publisher
Blomfield A, Maurya M, Bora K, Pavlovich M, Yemanyi F, Huang S, et al. Ectopic Rod Photoreceptor Development in Mice with Genetic Deficiency of WNT2B. Cells. 2023;12: pubmed publisher
Dhingra A, Tobias J, Philp N, Boesze Battaglia K. Transcriptomic Changes Predict Metabolic Alterations in LC3 Associated Phagocytosis in Aged Mice. Int J Mol Sci. 2023;24: pubmed publisher
Dhingra A, Tobias J, Philp N, Boesze Battaglia K. Transcriptomic changes predict metabolic alterations in LC3 associated phagocytosis in aged mice. bioRxiv. 2023;: pubmed publisher
Wu J, Cho C, Jo D, Kim J. Application of Base Editor-Mediated Genome Editing in Mouse Retina. Methods Mol Biol. 2023;2606:179-188 pubmed publisher
Jo D, Jang H, Cho C, Han J, Ryu G, Jung Y, et al. Visual function restoration in a mouse model of Leber congenital amaurosis via therapeutic base editing. Mol Ther Nucleic Acids. 2023;31:16-27 pubmed publisher
Ripolles Garcia A, Chen Y, Sato Y, Gray A, Ying G, Aguirre G, et al. Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations. Invest Ophthalmol Vis Sci. 2022;63:22 pubmed publisher
Tian H, Chen Z, Zhu X, Ou Q, Wang Z, Wu B, et al. Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration. iScience. 2022;25:105050 pubmed publisher
Sim xf3 n M, Vera M, Tenconi P, Soto T, Prado Spalm F, Torlaschi C, et al. Sphingosine-1-phosphate and ceramide-1-phosphate promote migration, pro-inflammatory and pro-fibrotic responses in retinal pigment epithelium cells. Exp Eye Res. 2022;224:109222 pubmed publisher
Nguyen Hoang A, Lee H, Lee S. Casein kinase I inhibitor D4476 influences autophagy and apoptosis in chloroquine-induced adult retinal pigment epithelial-19 cells. Exp Eye Res. 2022;218:109004 pubmed publisher
Nizawa T, Bhutto I, Tiwari A, Grebe R, Alt J, Rais R, et al. Topical Ketotifen Fumarate Inhibits Choroidal Mast Cell Degranulation and Loss of Retinal Pigment Epithelial Cells in Rat Model for Geographic Atrophy. Transl Vis Sci Technol. 2021;10:37 pubmed publisher
Zhang J, Gao F, Ma Y, Xue T, Shen Y. Identification of early-onset photoreceptor degeneration in transgenic mouse models of Alzheimer's disease. iScience. 2021;24:103327 pubmed publisher
Thomas B, Lin B, Martinez Camarillo J, Zhu D, McLelland B, Nistor G, et al. Co-grafts of Human Embryonic Stem Cell Derived Retina Organoids and Retinal Pigment Epithelium for Retinal Reconstruction in Immunodeficient Retinal Degenerate Royal College of Surgeons Rats. Front Neurosci. 2021;15:752958 pubmed publisher
Chae J, Jang H, Son C, Park C, Choi H, Jin S, et al. Targeting senescent retinal pigment epithelial cells facilitates retinal regeneration in mouse models of age-related macular degeneration. GeroScience. 2021;43:2809-2833 pubmed publisher
Jang H, Jo D, Lee S, Cho C, Jeong Y, Jung Y, et al. High-purity production and precise editing of DNA base editing ribonucleoproteins. Sci Adv. 2021;7: pubmed publisher
Zhao Q, Kong Y, Kittredge A, Li Y, Shen Y, Zhang Y, et al. Distinct expression requirements and rescue strategies for BEST1 loss- and gain-of-function mutations. elife. 2021;10: pubmed publisher
Kambhampati S, Bhutto I, Wu T, Ho K, McLeod D, Lutty G, et al. Systemic dendrimer nanotherapies for targeted suppression of choroidal inflammation and neovascularization in age-related macular degeneration. J Control Release. 2021;335:527-540 pubmed publisher
Tsai Y, Li Y, Ryu J, Su P, Cheng C, Wu W, et al. Impaired cholesterol efflux in retinal pigment epithelium of individuals with juvenile macular degeneration. Am J Hum Genet. 2021;108:903-918 pubmed publisher
You J, Cheng Y, Yang X, Chen L. Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system. Stem Cell Res. 2021;53:102342 pubmed publisher
Chen Y, Huang Y, Wu P, Chiang W, Wang P, Chen B. The Functional Vision Protection Effect of Danshensu via Dopamine D1 Receptors: In Vivo Study. Nutrients. 2021;13: pubmed publisher
Chen J, Sun J, Hu Y, Wan X, Wang Y, Gao M, et al. MicroRNA-191-5p ameliorates amyloid-β1-40 -mediated retinal pigment epithelium cell injury by suppressing the NLRP3 inflammasome pathway. FASEB J. 2021;35:e21184 pubmed publisher
Lee Rivera I, Lopez E, Alvarez Arce A, López Colomé A. The PKC-ζ pseudosubstrate peptide induces glutamate release from retinal pigment epithelium cells through kinase- independent activation of Best1. Life Sci. 2021;265:118860 pubmed publisher
Mitrousis N, Hacibekiroglu S, Ho M, Sauve Y, Nagy A, van der Kooy D, et al. Hydrogel-mediated co-transplantation of retinal pigmented epithelium and photoreceptors restores vision in an animal model of advanced retinal degeneration. Biomaterials. 2020;257:120233 pubmed publisher
Ogura S, Baldeosingh R, Bhutto I, Kambhampati S, Scott McLeod D, Edwards M, et al. A role for mast cells in geographic atrophy. FASEB J. 2020;34:10117-10131 pubmed publisher
Udry F, Decembrini S, Gamm D, D xe9 glon N, Kostic C, Arsenijevic Y. Lentiviral mediated RPE65 gene transfer in healthy hiPSCs-derived retinal pigment epithelial cells markedly increased RPE65 mRNA, but modestly protein level. Sci Rep. 2020;10:8890 pubmed publisher
Song J, Han D, Lee H, Kim D, Cho J, Park J, et al. A Comprehensive Proteomic and Phosphoproteomic Analysis of Retinal Pigment Epithelium Reveals Multiple Pathway Alterations in Response to the Inflammatory Stimuli. Int J Mol Sci. 2020;21: pubmed publisher
Lehmann G, Hanke Gogokhia C, Hu Y, Bareja R, Salfati Z, Ginsberg M, et al. Single-cell profiling reveals an endothelium-mediated immunomodulatory pathway in the eye choroid. J Exp Med. 2020;217: pubmed publisher
Yang C, Shani S, Tahiri H, Ortiz C, Gu M, Lavoie J, et al. Extracellular microparticles exacerbate oxidative damage to retinal pigment epithelial cells. Exp Cell Res. 2020;390:111957 pubmed publisher
Ji C, Li Y, Kittredge A, Hopiavuori A, Ward N, Yao P, et al. Investigation and Restoration of BEST1 Activity in Patient-derived RPEs with Dominant Mutations. Sci Rep. 2019;9:19026 pubmed publisher
Smith J, Ashander L, Ma Y, Rochet E, Furtado J. Model Systems for Studying Mechanisms of Ocular Toxoplasmosis. Methods Mol Biol. 2020;2071:297-321 pubmed publisher
Lopez E, Lee Rivera I, Alvarez Arce A, López Colomé A. Thrombin induces Ca2+-dependent glutamate release from RPE cells mediated by PLC/PKC and reverse Na+/Ca2+ exchange. Mol Vis. 2019;25:546-558 pubmed
Weed L, Ammar M, Zhou S, Wei Z, Serrano L, Sun J, et al. Safety of Same-Eye Subretinal Sequential Readministration of AAV2-hRPE65v2 in Non-human Primates. Mol Ther Methods Clin Dev. 2019;15:133-148 pubmed publisher
Gardiner K, Cideciyan A, Swider M, Dufour V, Sumaroka A, Komaromy A, et al. Long-Term Structural Outcomes of Late-Stage RPE65 Gene Therapy. Mol Ther. 2020;28:266-278 pubmed publisher
Lian C, Lou H, Zhang J, Tian H, Ou Q, Xu J, et al. MicroRNA-24 protects retina from degeneration in rats by down-regulating chitinase-3-like protein 1. Exp Eye Res. 2019;188:107791 pubmed publisher
Hellinen L, Pirskanen L, Tengvall Unadike U, Urtti A, Reinisalo M. Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties. Pharmaceutics. 2019;11: pubmed publisher
Wright T, Brisson B, Wood G, Oblak M, Mutsaers A, Sabine V, et al. Flow Cytometric Detection of Circulating Osteosarcoma Cells in Dogs. Cytometry A. 2019;95:997-1007 pubmed publisher
Scruggs B, Jiao C, Cranston C, Kaalberg E, Wang K, Russell S, et al. Optimizing Donor Cellular Dissociation and Subretinal Injection Parameters for Stem Cell-Based Treatments. Stem Cells Transl Med. 2019;8:797-809 pubmed publisher
Cai H, Gong J, Abriola L, Hoyer D, Nyscf Global Stem Cell Array Team -, Noggle S, et al. High-throughput screening identifies compounds that protect RPE cells from physiological stressors present in AMD. Exp Eye Res. 2019;185:107641 pubmed publisher
Cho I, Park S, Lee S, Nah S, Park H, Yang J, et al. The role of Wnt/β-catenin signaling in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium after laser photocoagulation. Lasers Med Sci. 2019;34:571-581 pubmed publisher
Bhutto I, Ogura S, Baldeosingh R, McLeod D, Lutty G, Edwards M. An Acute Injury Model for the Phenotypic Characteristics of Geographic Atrophy. Invest Ophthalmol Vis Sci. 2018;59:AMD143-AMD151 pubmed publisher
Kaur G, Tan L, Rathnasamy G, La Cunza N, Germer C, Toops K, et al. Aberrant early endosome biogenesis mediates complement activation in the retinal pigment epithelium in models of macular degeneration. Proc Natl Acad Sci U S A. 2018;115:9014-9019 pubmed publisher
Chae J, Rho C, Shin J, Lyu J, Kang S. Effects of Ranibizumab, Bevacizumab, and Aflibercept on Senescent Retinal Pigment Epithelial Cells. Korean J Ophthalmol. 2018;32:328-338 pubmed publisher
Gao Z, Liao Y, Chen C, Liao C, He D, Chen J, et al. Conversion of all-trans-retinal into all-trans-retinal dimer reflects an alternative metabolic/antidotal pathway of all-trans-retinal in the retina. J Biol Chem. 2018;293:14507-14519 pubmed publisher
Marmorstein A, Johnson A, Bachman L, Andrews Pfannkoch C, Knudsen T, Gilles B, et al. Mutant Best1 Expression and Impaired Phagocytosis in an iPSC Model of Autosomal Recessive Bestrophinopathy. Sci Rep. 2018;8:4487 pubmed publisher
Llonch S, Carido M, Ader M. Organoid technology for retinal repair. Dev Biol. 2018;433:132-143 pubmed publisher
Li Y, Zhang Y, Xu Y, Kittredge A, Ward N, Chen S, et al. Patient-specific mutations impair BESTROPHIN1's essential role in mediating Ca2+-dependent Cl- currents in human RPE. elife. 2017;6: pubmed publisher
Liao R, Yan F, Zeng Z, Wang H, Qiu K, Xu J, et al. IGF-1 activates PI3K/Akt signaling to protect human retinal pigment epithelial cells from amiodarone-induced oxidative injury. Br J Pharmacol. 2017;: pubmed publisher
Sun J, Huang P, Liang J, Li J, Shen M, She X, et al. Cooperation of Rel family members in regulating A?1-40-mediated pro-inflammatory cytokine secretion by retinal pigment epithelial cells. Cell Death Dis. 2017;8:e3115 pubmed publisher
Steinfeld J, Steinfeld I, Bausch A, Coronato N, Hampel M, Depner H, et al. BMP-induced reprogramming of the neural retina into retinal pigment epithelium requires Wnt signalling. Biol Open. 2017;6:979-992 pubmed publisher
Chowers G, Cohen M, Marks Ohana D, Stika S, Eijzenberg A, Banin E, et al. Course of Sodium Iodate-Induced Retinal Degeneration in Albino and Pigmented Mice. Invest Ophthalmol Vis Sci. 2017;58:2239-2249 pubmed publisher
Zheng W, Meng Q, Wang H, Yan F, Little P, Deng X, et al. IGF-1-Mediated Survival from Induced Death of Human Primary Cultured Retinal Pigment Epithelial Cells Is Mediated by an Akt-Dependent Signaling Pathway. Mol Neurobiol. 2018;55:1915-1927 pubmed publisher
Sudharsan R, Simone K, Anderson N, Aguirre G, Beltran W. Acute and Protracted Cell Death in Light-Induced Retinal Degeneration in the Canine Model of Rhodopsin Autosomal Dominant Retinitis Pigmentosa. Invest Ophthalmol Vis Sci. 2017;58:270-281 pubmed publisher
Zhou T, Rivera J, Bhosle V, Lahaie I, Shao Z, Tahiri H, et al. Choroidal Involution Is Associated with a Progressive Degeneration of the Outer Retinal Function in a Model of Retinopathy of Prematurity: Early Role for IL-1?. Am J Pathol. 2016;186:3100-3116 pubmed publisher
Tan L, Toops K, Lakkaraju A. Protective responses to sublytic complement in the retinal pigment epithelium. Proc Natl Acad Sci U S A. 2016;113:8789-94 pubmed publisher
Lyzogubov V, Bora P, Wu X, Horn L, de Roque R, Rudolf X, et al. The Complement Regulatory Protein CD46 Deficient Mouse Spontaneously Develops Dry-Type Age-Related Macular Degeneration-Like Phenotype. Am J Pathol. 2016;186:2088-2104 pubmed publisher
Iwabe S, Ying G, Aguirre G, Beltran W. Assessment of visual function and retinal structure following acute light exposure in the light sensitive T4R rhodopsin mutant dog. Exp Eye Res. 2016;146:341-53 pubmed publisher
Ayala Peña V, Pilotti F, Volonté Y, Rotstein N, Politi L, German O. Protective effects of retinoid x receptors on retina pigment epithelium cells. Biochim Biophys Acta. 2016;1863:1134-45 pubmed publisher
Chen M, Rajapakse D, Fraczek M, Luo C, Forrester J, Xu H. Retinal pigment epithelial cell multinucleation in the aging eye - a mechanism to repair damage and maintain homoeostasis. Aging Cell. 2016;15:436-45 pubmed publisher
Parker J, Mitrousis N, Shoichet M. Hydrogel for Simultaneous Tunable Growth Factor Delivery and Enhanced Viability of Encapsulated Cells in Vitro. Biomacromolecules. 2016;17:476-84 pubmed publisher
Cashman S, Gracias J, Adhi M, Kumar Singh R. Adenovirus-mediated delivery of Factor H attenuates complement C3 induced pathology in the murine retina: a potential gene therapy for age-related macular degeneration. J Gene Med. 2015;17:229-43 pubmed publisher
Lee S, Kim H, Park Y, Ohn Y, Park T. Time-Dependent Changes of Cell Proliferation After Laser Photocoagulation in Mouse Chorioretinal Tissue. Invest Ophthalmol Vis Sci. 2015;56:2696-708 pubmed publisher
Guziewicz K, Zangerl B, Komaromy A, Iwabe S, Chiodo V, Boye S, et al. Recombinant AAV-mediated BEST1 transfer to the retinal pigment epithelium: analysis of serotype-dependent retinal effects. PLoS ONE. 2013;8:e75666 pubmed publisher
Wallace C, Moir G, Malone D, Duncan L, Devarajan G, Crane I. Regulation of T-lymphocyte CCL3 and CCL4 production by retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 2013;54:722-30 pubmed publisher
Krohne T, Westenskow P, Kurihara T, Friedlander D, Lehmann M, Dorsey A, et al. Generation of retinal pigment epithelial cells from small molecules and OCT4 reprogrammed human induced pluripotent stem cells. Stem Cells Transl Med. 2012;1:96-109 pubmed
Hern ndez M, Pearce Kelling S, Rodriguez F, Aguirre G, Vecino E. Altered expression of retinal molecular markers in the canine RPE65 model of Leber congenital amaurosis. Invest Ophthalmol Vis Sci. 2010;51:6793-802 pubmed publisher
Sonoda S, Spee C, Barron E, Ryan S, Kannan R, Hinton D. A protocol for the culture and differentiation of highly polarized human retinal pigment epithelial cells. Nat Protoc. 2009;4:662-73 pubmed publisher
Jacobson A, Yan C, Gao Q, Rincon Skinner T, Rivera A, Edwards J, et al. Aging enhances pressure-induced arterial superoxide formation. Am J Physiol Heart Circ Physiol. 2007;293:H1344-50 pubmed
product information
master code :
NB100-355
SKU :
NB100-355
product name :
RPE65 Antibody (401.8B11.3D9) - BSA Free
unit size :
0.2 ml (also 0.05 ml)
description :
The RPE65 Antibody (401.8B11.3D9) - BSA Free from Novus is a mouse monoclonal antibody to RPE65. This antibody reacts with avian - chicken,bovine,canine,chicken,human,mouse,porcine,primate,rat,xenopus. The RPE65 Antibody (401.8B11.3D9) - BSA Free has been validated for the following applications: Immunohistochemistry,IF/IHC,Simple Western,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,Immunoprecipitation,In vivo assay,Immunofluorescence,CyTOF-ready,Knockout Validated.
target :
RPE65
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
401.8B11.3D9
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
This RPE65 Antibody (401.8B11.3D9) was developed against bovine RPE65 microsomal membrane proteins. [UniProt# Q28175]
isotype :
IgG1 Kappa
purity :
Protein G purified
species :
Avian - Chicken,Bovine,Canine,Chicken,Human,Mouse,Porcine,Primate,Rat,Xenopus
theoretical molecular weight :
65 kDa
gene symbol :
RPE65
Antibody validation :
Knockout/Knockdown
applications :
Immunohistochemistry,IF/IHC,Simple Western,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,Immunoprecipitation,In vivo assay,Immunofluorescence,CyTOF-ready,Knockout Validated
USD :
469 USD
alt names :
All-trans-retinyl-palmitate hydrolase, BCO family, member 3, BCO3, EC 3.1.1.64, EC:3.1.1.64, EC:5.3.3.22, LCA2, lutein isomerase, meso-zeaxanthin isomerase, mRPE65, p63, RBP-binding membrane protein, rd12, retinal pigment epithelium specific protein 65, Retinal pigment epithelium-specific 65 kDa protein, retinal pigment epithelium-specific protein 65kDa, retinitis pigmentosa 20 (autosomal recessive), retinoid isomerohydrolase, Retinol isomerase, RP20, RPE65, RPE65, retinoid isomerohydrolase, sRPE65
storage :
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.