product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
NDC80 Antibody (9G3.23) - Azide and BSA Free
catalog :
NB100-338
quantity :
0.1 ml
price :
449 USD
clonality :
monoclonal
host :
mouse
conjugate :
DyLight680
clone name :
9G3.23
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, proximity ligation assay
more info or order :
citations: 48
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:3000; loading ...; fig 6a
Deluca K, Meppelink A, Broad A, Mick J, Peersen O, Pektas S, et al. Aurora A kinase phosphorylates Hec1 to regulate metaphase kinetochore-microtubule dynamics. J Cell Biol. 2018;217:163-177 pubmed publisher
Roy B, Han S, Fontan A, Jema S, Joglekar A. Aurora B phosphorylates Bub1 to promote spindle assembly checkpoint signaling. Curr Biol. 2022;32:237-247.e6 pubmed publisher
Neahring L, Cho N, Dumont S. Opposing motors provide mechanical and functional robustness in the human spindle. Dev Cell. 2021;56:3006-3018.e5 pubmed publisher
Uchida K, Jo M, Nagasaka K, Takahashi M, Shindo N, Shibata K, et al. Kinetochore stretching-mediated rapid silencing of the spindle-assembly checkpoint required for failsafe chromosome segregation. Curr Biol. 2021;31:1581-1591.e3 pubmed publisher
Wimbish R, Deluca K, Mick J, Himes J, Jiménez Sánchez I, Jeyaprakash A, et al. The Hec1/Ndc80 tail domain is required for force generation at kinetochores, but is dispensable for kinetochore-microtubule attachment formation and Ska complex recruitment. Mol Biol Cell. 2020;31:1453-1473 pubmed publisher
Raab M, Sanhaji M, Matthess Y, Hörlin A, Lorenz I, Dötsch C, et al. PLK1 has tumor-suppressive potential in APC-truncated colon cancer cells. Nat Commun. 2018;9:1106 pubmed publisher
Dumitru A, Rusin S, Clark A, Kettenbach A, Compton D. Cyclin A/Cdk1 modulates Plk1 activity in prometaphase to regulate kinetochore-microtubule attachment stability. elife. 2017;6: pubmed publisher
Niikura Y, Kitagawa R, Ogi H, Kitagawa K. SGT1-HSP90 complex is required for CENP-A deposition at centromeres. Cell Cycle. 2017;16:1683-1694 pubmed publisher
Long A, Udy D, Dumont S. Hec1 Tail Phosphorylation Differentially Regulates Mammalian Kinetochore Coupling to Polymerizing and Depolymerizing Microtubules. Curr Biol. 2017;27:1692-1699.e3 pubmed publisher
Tauchman E, Boehm F, DeLuca J. Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells. Nat Commun. 2015;6:10036 pubmed publisher
Kleyman M, Kabeche L, Compton D. STAG2 promotes error correction in mitosis by regulating kinetochore-microtubule attachments. J Cell Sci. 2014;127:4225-33 pubmed publisher
Sivakumar S, Daum J, Tipton A, Rankin S, Gorbsky G. The spindle and kinetochore-associated (Ska) complex enhances binding of the anaphase-promoting complex/cyclosome (APC/C) to chromosomes and promotes mitotic exit. Mol Biol Cell. 2014;25:594-605 pubmed publisher
Yao R, Kondoh Y, Natsume Y, Yamanaka H, Inoue M, Toki H, et al. A small compound targeting TACC3 revealed its different spatiotemporal contributions for spindle assembly in cancer cells. Oncogene. 2014;33:4242-52 pubmed publisher
Kabeche L, Compton D. Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation. Nature. 2013;502:110-3 pubmed publisher
Maier B, Kirsch M, Anderhub S, Zentgraf H, Krämer A. The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells. Cell Cycle. 2013;12:1457-71 pubmed publisher
De Antoni A, Maffini S, Knapp S, Musacchio A, Santaguida S. A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B. J Cell Biol. 2012;199:269-84 pubmed publisher
Hwang M, Peddibhotla S, McHenry P, Chang P, Yochum Z, Park K, et al. P190B RhoGAP Regulates Chromosome Segregation in Cancer Cells. Cancers (Basel). 2012;4:475-89 pubmed publisher
Matson D, Demirel P, Stukenberg P, Burke D. A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint. Genes Dev. 2012;26:542-7 pubmed publisher
Chan Y, Jeyaprakash A, Nigg E, Santamaria A. Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction. J Cell Biol. 2012;196:563-71 pubmed publisher
Krenn V, Wehenkel A, Li X, Santaguida S, Musacchio A. Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction. J Cell Biol. 2012;196:451-67 pubmed publisher
Thompson S, Compton D. Chromosome missegregation in human cells arises through specific types of kinetochore-microtubule attachment errors. Proc Natl Acad Sci U S A. 2011;108:17974-8 pubmed publisher
Serio G, Margaria V, Jensen S, Oldani A, Bartek J, Bussolino F, et al. Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores. Proc Natl Acad Sci U S A. 2011;108:17337-42 pubmed publisher
Ma N, Titus J, Gable A, Ross J, Wadsworth P. TPX2 regulates the localization and activity of Eg5 in the mammalian mitotic spindle. J Cell Biol. 2011;195:87-98 pubmed publisher
Barnhart M, Kuich P, Stellfox M, Ward J, Bassett E, Black B, et al. HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore. J Cell Biol. 2011;194:229-43 pubmed publisher
Santaguida S, Vernieri C, Villa F, Ciliberto A, Musacchio A. Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction. EMBO J. 2011;30:1508-19 pubmed publisher
Screpanti E, De Antoni A, Alushin G, Petrovic A, Melis T, Nogales E, et al. Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore. Curr Biol. 2011;21:391-8 pubmed publisher
Tooley J, Miller S, Stukenberg P. The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement. Mol Biol Cell. 2011;22:1217-26 pubmed publisher
Sundin L, Guimaraes G, DeLuca J. The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis. Mol Biol Cell. 2011;22:759-68 pubmed publisher
Zanet J, Freije A, Ruiz M, Coulon V, Sanz J, Chiesa J, et al. A mitosis block links active cell cycle with human epidermal differentiation and results in endoreplication. PLoS ONE. 2010;5:e15701 pubmed publisher
Zeng K, Bastos R, Barr F, Gruneberg U. Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2. J Cell Biol. 2010;191:1315-32 pubmed publisher
Petrovic A, Pasqualato S, Dube P, Krenn V, Santaguida S, Cittaro D, et al. The MIS12 complex is a protein interaction hub for outer kinetochore assembly. J Cell Biol. 2010;190:835-52 pubmed publisher
Screpanti E, Santaguida S, Nguyen T, Silvestri R, Gussio R, Musacchio A, et al. A screen for kinetochore-microtubule interaction inhibitors identifies novel antitubulin compounds. PLoS ONE. 2010;5:e11603 pubmed publisher
Santaguida S, Tighe A, D Alise A, Taylor S, Musacchio A. Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. J Cell Biol. 2010;190:73-87 pubmed publisher
Wu G, Wei R, Cheng E, Ngo B, Lee W. Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1. Mol Biol Cell. 2009;20:4686-95 pubmed publisher
Yang Z, Kenny A, Brito D, Rieder C. Cells satisfy the mitotic checkpoint in Taxol, and do so faster in concentrations that stabilize syntelic attachments. J Cell Biol. 2009;186:675-84 pubmed publisher
Chan Y, Fava L, Uldschmid A, Schmitz M, Gerlich D, Nigg E, et al. Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. J Cell Biol. 2009;185:859-74 pubmed publisher
Cai S, Weaver L, Ems McClung S, Walczak C. Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules. Mol Biol Cell. 2009;20:1348-59 pubmed publisher
Zhu H, Coppinger J, Jang C, Yates J, Fang G. FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle. J Cell Biol. 2008;183:835-48 pubmed publisher
Miller S, Johnson M, Stukenberg P. Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1). Curr Biol. 2008;18:1785-91 pubmed publisher
Wu G, Qiu X, Zhou L, Zhu J, Chamberlin R, Lau J, et al. Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal. Cancer Res. 2008;68:8393-9 pubmed publisher
Barr A, Gergely F. MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics. Mol Cell Biol. 2008;28:7199-211 pubmed publisher
Jang C, Wong J, Coppinger J, Seki A, Yates J, Fang G. DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement. J Cell Biol. 2008;181:255-67 pubmed publisher
Wong J, Lerrigo R, Jang C, Fang G. Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domain. Mol Biol Cell. 2008;19:2083-91 pubmed publisher
Montembault E, Dutertre S, Prigent C, Giet R. PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores. J Cell Biol. 2007;179:601-9 pubmed
Wong J, Fang G. HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture. J Cell Biol. 2006;173:879-91 pubmed
Klein U, Nigg E, Gruneberg U. Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP. Mol Biol Cell. 2006;17:2547-58 pubmed
Ciferri C, De Luca J, Monzani S, Ferrari K, Ristic D, Wyman C, et al. Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. J Biol Chem. 2005;280:29088-95 pubmed
Ganem N, Compton D. The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK. J Cell Biol. 2004;166:473-8 pubmed
product information
master code :
NB100-338
SKU :
NB100-338
product name :
NDC80 Antibody (9G3.23) - Azide and BSA Free
unit size :
0.1 ml
description :
The NDC80 Antibody (9G3.23) - Azide and BSA Free from Novus is a mouse monoclonal antibody to NDC80. This antibody reacts with hamster,human,marsupial,mouse. The NDC80 Antibody (9G3.23) - Azide and BSA Free has been validated for the following applications: Western Blot,Immunocytochemistry/ Immunofluorescence,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Immunohistochemistry-Frozen,Proximity Ligation Assay.
target :
NDC80
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
9G3.23
conjugate :
Unconjugated
host :
Mouse
immunogen :
Human NDC80 protein consisting of amino acids 56-642.
isotype :
IgG2a
purity :
Protein A purified
species :
Hamster,Human,Marsupial,Mouse
theoretical molecular weight :
80 kDa
gene symbol :
NDC80
Antibody validation :
Orthogonal Validation
accessionNumbers :
O14777
applications :
Western Blot,Immunocytochemistry/ Immunofluorescence,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Immunohistochemistry-Frozen,Proximity Ligation Assay
USD :
449 USD
alt names :
HEC1KNTC2, HECRetinoblastoma-associated protein HEC, Highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, highly expressed in cancer, rich in leucine heptad repeats (yeast), hsHec1, hsNDC80, kinetochore associated 2, Kinetochore protein Hec1, kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, NDC80 homolog, kinetochore complex component (S. cerevisiae), TID3
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.