TERT Antibody (2C4) - BSA Free | Novus Biologicals NB100-317 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

TERT Antibody (2C4) - BSA Free

catalog :

NB100-317

quantity :

0.1 ml (also 0.025 ml)

price :

489 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2C4

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Novus Biologicals product webpage

citations: 75

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; loading ...; fig 3a western blot; mouse; loading ...; fig 3b	Wu X, Yang Y, Li W, Cheng Y, Li X, Huang C, et al. Telomerase reverse transcriptase acts in a feedback loop with NF-ÎºB pathway to regulate macrophage polarization in alcoholic liver disease. Sci Rep. 2016;6:18685 pubmed publisher
immunohistochemistry - paraffin section; human	Lotfi R, El Zawahry K, Kamar Z, Hashem Z. Effects of smoking on human telomerase reverse transcriptase expression in the skin. Int J Dermatol. 2014;53:1205-12 pubmed publisher
western blot; mouse	Dosset M, Godet Y, Vauchy C, Beziaud L, Lone Y, Sedlik C, et al. Universal cancer peptide-based therapeutic vaccine breaks tolerance against telomerase and eradicates established tumor. Clin Cancer Res. 2012;18:6284-95 pubmed publisher
immunohistochemistry; human	Kim M, Chen Y, Bekele B, Lopez A, Khanna A, Chen J, et al. Activating enhancer-binding protein-2? nucleolar localization predicts poor survival after stage I non-small cell lung cancer resection. Ann Thorac Surg. 2011;92:1044-50 pubmed publisher
immunohistochemistry; human	Fabricius E, Kruse Boitschenko U, Khoury R, Wildner G, Raguse J, Klein M, et al. Localization of telomerase hTERT protein in frozen sections of basal cell carcinomas (BCC) and tumor margin tissues. Int J Oncol. 2009;35:1377-94 pubmed
immunohistochemistry; human	Fabricius E, Kruse Boitschenko U, Khoury R, Wildner G, Raguse J, Klein M. Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues. Int J Oncol. 2009;34:1257-79 pubmed
western blot; human	Masutomi K, Possemato R, Wong J, Currier J, Tothova Z, Manola J, et al. The telomerase reverse transcriptase regulates chromatin state and DNA damage responses. Proc Natl Acad Sci U S A. 2005;102:8222-7 pubmed
immunoprecipitation; human western blot; human	Masutomi K, Yu E, Khurts S, Ben Porath I, Currier J, Metz G, et al. Telomerase maintains telomere structure in normal human cells. Cell. 2003;114:241-53 pubmed
	Liu T, Long Q, Li L, Gan H, Hu X, Long H, et al. The NRF2-dependent transcriptional axis, XRCC5/hTERT drives tumor progression and 5-Fu insensitivity in hepatocellular carcinoma. Mol Ther Oncolytics. 2022;24:249-261 pubmed publisher
	COLLINS D, Osborn M, Steer C. Differentiation of immortalized human multi-lineage progenitor to alveolar type 2-like cells: angiotensin-converting enzyme 2 expression and binding of severe acute respiratory syndrome coronavirus 2 spike and spike 1 proteins. Cytotherapy. 2021;23:1064-1073 pubmed publisher
	del Moral Hernández O, Hernández Sotelo D, Alarcón Romero L, Mendoza Catalán M, Flores Alfaro E, Castro Coronel Y, et al. TOP2A/MCM2, p16INK4a, and cyclin E1 expression in liquid-based cytology: a biomarkers panel for progression risk of cervical premalignant lesions. BMC Cancer. 2021;21:39 pubmed publisher
	Kubo C, Ogawa M, Uehara N, Katakura Y. Fisetin Promotes Hair Growth by Augmenting TERT Expression. Front Cell Dev Biol. 2020;8:566617 pubmed publisher
	Zhang X, Bai J, Yin H, Long L, Zheng Z, Wang Q, et al. Exosomal miR-1255b-5p targets human telomerase reverse transcriptase in colorectal cancer cells to suppress epithelial-to-mesenchymal transition. Mol Oncol. 2020;14:2589-2608 pubmed publisher
	COLLINS D, Hapke J, Aravalli R, Steer C. In vitro Differentiation of Human TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells. Hepat Med. 2020;12:79-92 pubmed publisher
	Kokubun T, Saitoh S, Miura S, Ishida T, Takeishi Y. Telomerase Plays a Pivotal Role in Collateral Growth Under Ischemia by Suppressing Age-Induced Oxidative Stress, Expression of p53, and Pro-Apoptotic Proteins. Int Heart J. 2019;60:736-745 pubmed publisher
	Wei J, Yang Q, Shi J, Shi B, Ji M, Hou P. Increased expression of NAF1 contributes to malignant phenotypes of glioma cells through promoting protein synthesis and associates with poor patient survival. Oncogenesis. 2019;8:25 pubmed publisher
	Giacalone J, Miller M, Workalemahu G, Reutzel A, Ochoa D, Whitmore S, et al. Generation of an immortalized human choroid endothelial cell line (iChEC-1) using an endothelial cell specific promoter. Microvasc Res. 2019;123:50-57 pubmed publisher
	Lee S, Shin S, Lee S, Kang J, Kook M, Kim I, et al. Phase I trial of intravenous Ad5CRT in patients with liver metastasis of gastrointestinal cancers. Cancer Gene Ther. 2019;26:174-178 pubmed publisher
	De S, Karim F, Kiessu E, Cushing L, Lin L, Ghandil P, et al. Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling. J Biol Chem. 2018;293:15208-15220 pubmed publisher
	Abdi K, Lai C, Paez Gonzalez P, Lay M, Pyun J, Kuo C. Uncovering inherent cellular plasticity of multiciliated ependyma leading to ventricular wall transformation and hydrocephalus. Nat Commun. 2018;9:1655 pubmed publisher
	Margalef P, Kotsantis P, Borel V, Bellelli R, Panier S, Boulton S. Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe. Cell. 2018;172:439-453.e14 pubmed publisher
	Kirkley K, Walton K, Duncan C, Tjalkens R. Spontaneous Development of Cutaneous Squamous Cell Carcinoma in Mice with Cell-specific Deletion of Inhibitor of κB Kinase 2. Comp Med. 2017;67:407-415 pubmed
	Cleary M, Mansoor A, Settelmeyer T, Ijiri Y, Ladner K, Svalina M, et al. NF?B signaling in alveolar rhabdomyosarcoma. Dis Model Mech. 2017;10:1109-1115 pubmed publisher
	Liu J, Ibi D, Taniguchi K, Lee J, Herrema H, Akosman B, et al. Inflammation Improves Glucose Homeostasis through IKKÎ²-XBP1s Interaction. Cell. 2016;167:1052-1066.e18 pubmed publisher
	Biron Shental T, Liberman M, Elbaz M, Laish I, Sharony R, Amiel A. Telomere homeostasis in placentas from pregnancies with uncontrolled diabetes. Placenta. 2016;44:13-8 pubmed publisher
	Chen J, Yao Z, Chen J, Gi Y, MuÃ±oz N, Kundra S, et al. TGF-Î²/Î²2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndrome. J Clin Invest. 2016;126:527-42 pubmed publisher
	Buhrmann C, Büsch F, Shayan P, Shakibaei M. Sirtuin-1 (SIRT1) is required for promoting chondrogenic differentiation of mesenchymal stem cells. J Biol Chem. 2014;289:22048-62 pubmed publisher
	Das U, Manna K, Sinha M, Datta S, Das D, Chakraborty A, et al. Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS ONE. 2014;9:e97599 pubmed publisher
	Paul S, Traver M, Kashyap A, Washington M, Latoche J, Schaefer B. T cell receptor signals to NF-κB are transmitted by a cytosolic p62-Bcl10-Malt1-IKK signalosome. Sci Signal. 2014;7:ra45 pubmed publisher
	Breuer R, Becker M, Brechmann M, Mock T, Arnold R, Krammer P. The protein phosphatase 2A regulatory subunit B56? mediates suppression of T cell receptor (TCR)-induced nuclear factor-?B (NF-?B) activity. J Biol Chem. 2014;289:14996-5004 pubmed publisher
	Gray C, Remouchamps C, McCorkell K, Solt L, Dejardin E, Orange J, et al. Noncanonical NF-?B signaling is limited by classical NF-?B activity. Sci Signal. 2014;7:ra13 pubmed publisher
	Ren T, Takahashi Y, Liu X, Loughran T, Sun S, Wang H, et al. HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains. Oncogene. 2015;34:334-45 pubmed publisher
	Bimonte S, Barbieri A, Palma G, Luciano A, Rea D, Arra C. Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer. Biomed Res Int. 2013;2013:810423 pubmed publisher
	Delhase M, Kim S, Lee H, Naiki Ito A, Chen Y, Ahn E, et al. TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2. Proc Natl Acad Sci U S A. 2012;109:E177-86 pubmed publisher
	Page A, Cascallana J, Casanova M, Navarro M, Alameda J, Perez P, et al. IKK? overexpression leads to pathologic lesions in stratified epithelia and exocrine glands and to tumoral transformation of oral epithelia. Mol Cancer Res. 2011;9:1329-38 pubmed publisher
	Takigami I, Ohno T, Kitade Y, Hara A, Nagano A, Kawai G, et al. Synthetic siRNA targeting the breakpoint of EWS/Fli-1 inhibits growth of Ewing sarcoma xenografts in a mouse model. Int J Cancer. 2011;128:216-26 pubmed publisher
	Harikumar K, Sung B, Pandey M, Guha S, Krishnan S, Aggarwal B. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway. Mol Pharmacol. 2010;77:818-27 pubmed publisher
	Song L, Ponomareva L, Shen H, Duan X, Alimirah F, Choubey D. Interferon-inducible IFI16, a negative regulator of cell growth, down-regulates expression of human telomerase reverse transcriptase (hTERT) gene. PLoS ONE. 2010;5:e8569 pubmed publisher
	Zhang Q, Shi S, Liu Y, Uyanne J, Shi Y, Shi S, et al. Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitis. J Immunol. 2009;183:7787-98 pubmed publisher
	Jin H, Lee D, Kim D, Chung J, Lee S, Lee T. cIAP1, cIAP2, and XIAP act cooperatively via nonredundant pathways to regulate genotoxic stress-induced nuclear factor-kappaB activation. Cancer Res. 2009;69:1782-91 pubmed publisher
	Koch K, Maeda S, He G, Karin M, Leffert H. Targeted deletion of hepatocyte Ikkbeta confers growth advantages. Biochem Biophys Res Commun. 2009;380:349-54 pubmed publisher
	Huang J, Ren T, Guan H, Jiang Y, Cheng H. HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaB. J Biol Chem. 2009;284:6208-17 pubmed publisher
	Pandey M, Sung B, Kunnumakkara A, Sethi G, Chaturvedi M, Aggarwal B. Berberine modifies cysteine 179 of IkappaBalpha kinase, suppresses nuclear factor-kappaB-regulated antiapoptotic gene products, and potentiates apoptosis. Cancer Res. 2008;68:5370-9 pubmed publisher
	Kasinski A, Du Y, Thomas S, Zhao J, Sun S, Khuri F, et al. Inhibition of IkappaB kinase-nuclear factor-kappaB signaling pathway by 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24), a novel monoketone analog of curcumin. Mol Pharmacol. 2008;74:654-61 pubmed publisher
	Lamothe B, Webster W, Gopinathan A, Besse A, Campos A, Darnay B. TRAF6 ubiquitin ligase is essential for RANKL signaling and osteoclast differentiation. Biochem Biophys Res Commun. 2007;359:1044-9 pubmed
	Beraza N, Ludde T, Assmus U, Roskams T, Vander Borght S, Trautwein C. Hepatocyte-specific IKK gamma/NEMO expression determines the degree of liver injury. Gastroenterology. 2007;132:2504-17 pubmed
	Vunta H, Davis F, Palempalli U, Bhat D, Arner R, Thompson J, et al. The anti-inflammatory effects of selenium are mediated through 15-deoxy-Delta12,14-prostaglandin J2 in macrophages. J Biol Chem. 2007;282:17964-73 pubmed
	Leis H, Sanchis A, Perez P. Deletion of the N-terminus of IKKgamma induces apoptosis in keratinocytes and impairs the AKT/PTEN signaling pathway. Exp Cell Res. 2007;313:742-52 pubmed
	Jackson Bernitsas D, Ichikawa H, Takada Y, Myers J, Lin X, Darnay B, et al. Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma. Oncogene. 2007;26:1385-97 pubmed
	Sethi G, Ahn K, Sandur S, Lin X, Chaturvedi M, Aggarwal B. Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway. J Biol Chem. 2006;281:23425-35 pubmed
	Sandur S, Ichikawa H, Sethi G, Ahn K, Aggarwal B. Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) suppresses NF-kappaB activation and NF-kappaB-regulated gene products through modulation of p65 and IkappaBalpha kinase activation, leading to potentiation of apoptosis induced by cytokine and chemoth. J Biol Chem. 2006;281:17023-33 pubmed
	Ichikawa H, Aggarwal B. Guggulsterone inhibits osteoclastogenesis induced by receptor activator of nuclear factor-kappaB ligand and by tumor cells by suppressing nuclear factor-kappaB activation. Clin Cancer Res. 2006;12:662-8 pubmed
	Nenci A, Huth M, Funteh A, Schmidt Supprian M, Bloch W, Metzger D, et al. Skin lesion development in a mouse model of incontinentia pigmenti is triggered by NEMO deficiency in epidermal keratinocytes and requires TNF signaling. Hum Mol Genet. 2006;15:531-42 pubmed
	Takada Y, Gillenwater A, Ichikawa H, Aggarwal B. Suberoylanilide hydroxamic acid potentiates apoptosis, inhibits invasion, and abolishes osteoclastogenesis by suppressing nuclear factor-kappaB activation. J Biol Chem. 2006;281:5612-22 pubmed
	Herrmann O, Baumann B, De Lorenzi R, Muhammad S, Zhang W, Kleesiek J, et al. IKK mediates ischemia-induced neuronal death. Nat Med. 2005;11:1322-9 pubmed
	Shishodia S, Gutierrez A, Lotan R, Aggarwal B. N-(4-hydroxyphenyl)retinamide inhibits invasion, suppresses osteoclastogenesis, and potentiates apoptosis through down-regulation of I(kappa)B(alpha) kinase and nuclear factor-kappaB-regulated gene products. Cancer Res. 2005;65:9555-65 pubmed
	Shishodia S, Amin H, Lai R, Aggarwal B. Curcumin (diferuloylmethane) inhibits constitutive NF-kappaB activation, induces G1/S arrest, suppresses proliferation, and induces apoptosis in mantle cell lymphoma. Biochem Pharmacol. 2005;70:700-13 pubmed
	Ichikawa H, Takada Y, Murakami A, Aggarwal B. Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products. J Immunol. 2005;174:7383-92 pubmed
	Luedde T, Assmus U, Wüstefeld T, Meyer zu Vilsendorf A, Roskams T, Schmidt Supprian M, et al. Deletion of IKK2 in hepatocytes does not sensitize these cells to TNF-induced apoptosis but protects from ischemia/reperfusion injury. J Clin Invest. 2005;115:849-59 pubmed
	Khurts S, Masutomi K, Delgermaa L, Arai K, Oishi N, Mizuno H, et al. Nucleolin interacts with telomerase. J Biol Chem. 2004;279:51508-15 pubmed
	Shishodia S, Aggarwal B. Guggulsterone inhibits NF-kappaB and IkappaBalpha kinase activation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis. J Biol Chem. 2004;279:47148-58 pubmed
	Shishodia S, Koul D, Aggarwal B. Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF-induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma: correlation with suppression of COX-2 synthesis. J Immunol. 2004;173:2011-22 pubmed
	Takada Y, Fang X, Jamaluddin M, Boyd D, Aggarwal B. Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor. J Biol Chem. 2004;279:39541-54 pubmed
	Takada Y, Khuri F, Aggarwal B. Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated gene expression and up-regulation of apoptosis. J Biol Chem. 2004;279:26287-99 pubmed
	Takada Y, Singh S, Aggarwal B. Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis. J Biol Chem. 2004;279:15096-104 pubmed
	Takada Y, Aggarwal B. Flavopiridol inhibits NF-kappaB activation induced by various carcinogens and inflammatory agents through inhibition of IkappaBalpha kinase and p65 phosphorylation: abrogation of cyclin D1, cyclooxygenase-2, and matrix metalloprotease-9. J Biol Chem. 2004;279:4750-9 pubmed
	Leis H, Page A, Ramirez A, Bravo A, Segrelles C, Paramio J, et al. Glucocorticoid Receptor Counteracts Tumorigenic Activity of Akt in Skin through Interference with the Phosphatidylinositol 3-Kinase Signaling Pathway. Mol Endocrinol. 2004;18:303-11 pubmed
	Majumdar S, Aggarwal B. Adenosine suppresses activation of nuclear factor-kappaB selectively induced by tumor necrosis factor in different cell types. Oncogene. 2003;22:1206-18 pubmed
	Santos M, Perez P, Segrelles C, Ruiz S, Jorcano J, Paramio J. Impaired NF-kappa B activation and increased production of tumor necrosis factor alpha in transgenic mice expressing keratin K10 in the basal layer of the epidermis. J Biol Chem. 2003;278:13422-30 pubmed
	Ashikawa K, Majumdar S, Banerjee S, Bharti A, Shishodia S, Aggarwal B. Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. J Immunol. 2002;169:6490-7 pubmed
	Anto R, Mukhopadhyay A, Shishodia S, Gairola C, Aggarwal B. Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2. Carcinogenesis. 2002;23:1511-8 pubmed
	Majumdar S, Lamothe B, Aggarwal B. Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester. J Immunol. 2002;168:2644-51 pubmed
	Majumdar S, Aggarwal B. Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation. J Immunol. 2001;167:2911-20 pubmed
	Miller B, Zandi E. Complete reconstitution of human IkappaB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKgamma on the complex activity in the absence of stimulation. J Biol Chem. 2001;276:36320-6 pubmed
	Manna S, Mukhopadhyay A, Aggarwal B. Leflunomide suppresses TNF-induced cellular responses: effects on NF-kappa B, activator protein-1, c-Jun N-terminal protein kinase, and apoptosis. J Immunol. 2000;165:5962-9 pubmed

product information

master code :

NB100-317

SKU :

NB100-317

product name :

TERT Antibody (2C4) - BSA Free

unit size :

0.1 ml (also 0.025 ml)

description :

The TERT Antibody (2C4) - BSA Free from Novus is a mouse monoclonal antibody to TERT. This antibody reacts with human,mouse,rat. The TERT Antibody (2C4) - BSA Free has been validated for the following applications: Immunoprecipitation,Western Blot,Immunohistochemistry,Chemotaxis,IF/IHC,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Chromatin Immunoprecipitation,Flow Cytometry,Chromatin Immunoprecipitation (ChIP).

target :

TERT

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

2C4

concentration :

1.0 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

Full-length recombinant human Telomerase reverse transcriptase (TERT) from insect cells. [Uniprot: O14746].

isotype :

IgM

purity :

IgM purified

species :

Human,Mouse,Rat

theoretical molecular weight :

127 kDa

gene symbol :

TERT

accessionNumbers :

O14746

applications :

Immunohistochemistry,Chemotaxis,IF/IHC,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Chromatin Immunoprecipitation,Flow Cytometry,Chromatin Immunoprecipitation (ChIP)

USD :

489 USD

alt names :

EST2Telomerase-associated protein 2, hEST2, hTERT, TCS1EC 2.7.7.49, Telomerase catalytic subunit, telomerase reverse transcriptase, TP2HEST2, TRTEC 2.7.7

storage :

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage