product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rabbit Polyclonal EGLN3/PHD3 Antibody
catalog :
NB100-303R
quantity :
0.1 ml
price :
319.00 USD
clonality :
polyclonal
host :
rabbit
conjugate :
DyLight 550
reactivity :
human, mouse, rat
application :
western blot
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB100-303R
Product Name :
Rabbit Polyclonal EGLN3/PHD3 Antibody
List Price :
319.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
EC 1.14.11, EC 1.14.11.-, EGL nine (C.elegans) homolog 3, egl nine homolog 3, egl nine homolog 3 (C. elegans), egl nine-like protein 3 isoform, FLJ21620, HIF prolyl hydroxylase 3, HIF-PH3, HIFPH3 MGC125998, HIF-prolyl hydroxylase 3, HPH-1, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 3, MGC125999, PHD3, Prolyl hydroxylase domain-containing protein 3
Background :
HIF prolyl hydroxylase 3 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HIF prolyl hydroxylase 1, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(564) and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may also play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissues, promoting cell death through a caspase-dependent mechanism.
Buffer :
PBS
Clonality :
Polyclonal
Conjugate :
DyLight 550
Application Summary :
Western Blot
Host :
Rabbit
Immunogen :
A synthetic peptide made to the C-terminus of humanPHD3/HIF Prolyl Hydroxylase 3. [LocusLink ID 112399]
Preservative :
No Preservative
Purity :
Immunogen affinity purified
CrossReactivity :
Human and mouse. Primate reactivity reported in scientific literature (PMID: 23732909). mmunogen sequence has 92% homology to rat.
Storage :
Store at 4C in the dark.
Swissprot Human :
Q9H6Z9
TMW :
27 kDa
Uses :
This PHD3/HIF Prolyl Hydroxylase 3 antibody is useful for Immunoprecipitation and Western blot, where a band is seen at ~27 kDa. This antibody has been tested against HeLa and MEF cell lysates. Immunoprecipitation was reported in PMID: 21575608. Chromatin Immunoprecipitation was reported in scientific literature.
Research Area :
Apoptosis, Cancer, Hypoxia
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.