product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Mouse Monoclonal HIF-1 alpha Antibody
catalog :
NB100-296R
quantity :
0.1 ml
price :
329 USD
clonality :
monoclonal
host :
mouse
reactivity :
human
application :
western blot
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB100-296R
Product Name :
Mouse Monoclonal HIF-1 alpha Antibody
List Price :
329 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
Background :
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
Buffer :
50mM sodium borate
Clonality :
Monoclonal
Clone :
HA111
Concentration :
1.1 mg/ml
Conjugate :
DyLight 550
Application Summary :
Western Blot
Host :
Mouse
Immunogen :
Human HIF-1 alpha, corresponding to amino acids 329-530. [UniProt# Q16665].
Isotype :
IgG2 Alpha
Localization :
Cytoplasmic in normoxia, nuclear translocation in response to hypoxia.
Preservative :
No Preservative
Purity :
Protein G purified
CrossReactivity :
Human
Storage :
Store at 4C in the dark.
Swissprot Human :
Q16665
Uses :
This HIF-1 alpha (HA111) antibody is useful for Western Blot and Immunohistochemistry on paraffin-embedded sections. Use in Immunoprecipitation was reported in scientific literature (PMID: 18222538). In IHC-P, staining was observed in the nucleus of human ovarian cancer tumor. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. **Nuclear extracts should be used for Western blot analysis.
Research Area :
Angiogenesis, Cancer, Chromatin Research, Hypoxia, Transcription Factors and Regulators
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.