Mouse Monoclonal MDM2/HDM2 Antibody (SMP14) [DyLight 550] | Novus Biologicals NB100-2736R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Mouse Monoclonal MDM2/HDM2 Antibody (SMP14) [DyLight 550]

catalog :

NB100-2736R

quantity :

0.1 ml

price :

369 USD

clonality :

monoclonal

host :

mouse

conjugate :

DyLight 550

clone name :

SMP14

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

product information

Catalog Number :

NB100-2736R

Product Name :

Mouse Monoclonal MDM2/HDM2 Antibody (SMP14) [DyLight 550]

List Price :

369 USD

product type :

Primary Antibodies

Package Size :

0.1 ml

AltNames :

ACTFS, HDM2, HDMX, MDM2 oncogene, E3 ubiquitin protein ligase

Buffer :

50mM Sodium Borate

Clonality :

Monoclonal

Clone :

SMP14

Conjugate :

DyLight 550

Application Summary :

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Epitope :

Amino acids 154 - 167.

Host :

Mouse

Immunogen :

Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProt# Q00987]

Isotype :

IgG1 Kappa

Localization :

Nuclear and cytoplasmic (expressed predominantly in the nucleoplasm).

Notes :

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Preservative :

0.05% Sodium Azide

Purity :

Protein G purified

CrossReactivity :

Human, Rat

Specificity :

The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

Storage :

Store at 4C in the dark.

Swissprot Human :

Q00987

Uses :

Immunohistochemistry - Frozen and Paraffin, Immunoprecipitation and Western blot. This antibody has also been reported useful in IHC on resin tissue sections. Staining of formalin-fixed paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Research Area :

Cancer, Cell Cycle and Replication, Cellular Markers, Hypoxia, Neuroscience, p53 Pathway, Phospho Specific, Tumor Suppressors