Mouse Monoclonal MDM2/HDM2 Antibody (SMP14) [Alexa Fluor® 488] | Novus Biologicals NB100-2736AF488 product information

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product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Mouse Monoclonal MDM2/HDM2 Antibody (SMP14) [Alexa Fluor® 488]

catalog :

NB100-2736AF488

quantity :

0.1 ml

price :

369 USD

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

SMP14

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

product information

Catalog Number :

NB100-2736AF488

Product Name :

Mouse Monoclonal MDM2/HDM2 Antibody (SMP14) [Alexa Fluor® 488]

List Price :

369 USD

product type :

Primary Antibodies

Package Size :

0.1 ml

AltNames :

ACTFS, HDM2, HDMX, MDM2 oncogene, E3 ubiquitin protein ligase

Buffer :

50mM Sodium Borate

Clonality :

Monoclonal

Clone :

SMP14

Conjugate :

Alexa Fluor 488

Application Summary :

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Epitope :

Amino acids 154 - 167.

Host :

Mouse

Immunogen :

Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProt# Q00987]

Isotype :

IgG1 Kappa

Localization :

Nuclear and cytoplasmic (expressed predominantly in the nucleoplasm).

Notes :

Alexa Flour (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Preservative :

0.05% Sodium Azide

Purity :

Protein G purified

CrossReactivity :

Human, Rat

Specificity :

The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

Storage :

Store at 4C in the dark.

Swissprot Human :

Q00987

Uses :

Immunohistochemistry - Frozen and Paraffin, Immunoprecipitation and Western blot. This antibody has also been reported useful in IHC on resin tissue sections. Staining of formalin-fixed paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Research Area :

Cancer, Cell Cycle and Replication, Cellular Markers, Hypoxia, Neuroscience, p53 Pathway, Phospho Specific, Tumor Suppressors