MDM2/HDM2 Antibody (SMP14) - BSA Free | Novus Biologicals NB100-2736 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

MDM2/HDM2 Antibody (SMP14) - BSA Free

catalog :

NB100-2736

quantity :

0.1 ml (also 0.025 ml)

price :

409 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SMP14

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Novus Biologicals product webpage

citations: 35

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse western blot; mouse	Joshi Y, SÃ³ria M, Quadrato G, Inak G, Zhou L, Hervera A, et al. The MDM4/MDM2-p53-IGF1 axis controls axonal regeneration, sprouting and functional recovery after CNS injury. Brain. 2015;138:1843-62 pubmed publisher
western blot; human	Inaba H, Kuboniwa M, Sugita H, Lamont R, Amano A. Identification of signaling pathways mediating cell cycle arrest and apoptosis induced by Porphyromonas gingivalis in human trophoblasts. Infect Immun. 2012;80:2847-57 pubmed publisher
immunohistochemistry; human	de Freitas M, Ramalho L, Xavier F, Moreira A, Reis S. p53 and MDM2 protein expression in actinic cheilitis. J Appl Oral Sci. 2008;16:414-9 pubmed
western blot; human	Chen D, Zhang Z, Li M, Wang W, Li Y, Rayburn E, et al. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function. Oncogene. 2007;26:5029-37 pubmed
immunoprecipitation; human	Stevenson L, Sparks A, Allende Vega N, Xirodimas D, Lane D, Saville M. The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2. EMBO J. 2007;26:976-86 pubmed
immunohistochemistry; rat	Pääjärvi G, Roudier E, Crisby M, Hogberg J, Stenius U. HMG-CoA reductase inhibitors, statins, induce phosphorylation of Mdm2 and attenuate the p53 response to DNA damage. FASEB J. 2005;19:476-8 pubmed
immunoprecipitation; human western blot; human	Minsky N, Oren M. The RING domain of Mdm2 mediates histone ubiquitylation and transcriptional repression. Mol Cell. 2004;16:631-9 pubmed
western blot; human	Menendez S, Higgins M, Berkson R, Edling C, Lane D, Lain S. Nuclear export inhibitor leptomycin B induces the appearance of novel forms of human Mdm2 protein. Br J Cancer. 2003;88:636-43 pubmed
western blot; human	Goldberg Z, Vogt Sionov R, Berger M, Zwang Y, Perets R, Van Etten R, et al. Tyrosine phosphorylation of Mdm2 by c-Abl: implications for p53 regulation. EMBO J. 2002;21:3715-27 pubmed
immunohistochemistry - paraffin section; human	Kersemaekers A, Mayer F, Molier M, Van Weeren P, Oosterhuis J, Bokemeyer C, et al. Role of P53 and MDM2 in treatment response of human germ cell tumors. J Clin Oncol. 2002;20:1551-61 pubmed
western blot; human	Hjerrild M, Milne D, Dumaz N, Hay T, Issinger O, Meek D. Phosphorylation of murine double minute clone 2 (MDM2) protein at serine-267 by protein kinase CK2 in vitro and in cultured cells. Biochem J. 2001;355:347-56 pubmed
western blot; human	Rodriguez Lopez A, Xenaki D, Eden T, Hickman J, Chresta C. MDM2 mediated nuclear exclusion of p53 attenuates etoposide-induced apoptosis in neuroblastoma cells. Mol Pharmacol. 2001;59:135-43 pubmed
western blot; human	Berger M, Vogt Sionov R, Levine A, Haupt Y. A role for the polyproline domain of p53 in its regulation by Mdm2. J Biol Chem. 2001;276:3785-90 pubmed
western blot; human	Sionov R, Moallem E, Berger M, Kazaz A, Gerlitz O, Ben Neriah Y, et al. c-Abl neutralizes the inhibitory effect of Mdm2 on p53. J Biol Chem. 1999;274:8371-4 pubmed
immunoprecipitation; human western blot; human	Shieh S, Ikeda M, Taya Y, Prives C. DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2. Cell. 1997;91:325-34 pubmed
	Lee S, Jeong H, Hwang B, Yu B, Cho Y, Nam K, et al. Helicobacter pylori promotes epithelial-to-mesenchymal transition by downregulating CK2β in gastric cancer cells. Biochim Biophys Acta Mol Basis Dis. 2022;1869:166588 pubmed publisher
	K x131 yga E, Ad x131 g xfc zel Z, xd6 nay U xe7 ar E. Temozolomide increases heat shock proteins in extracellular vesicles released from glioblastoma cells. Mol Biol Rep. 2022;49:8701-8713 pubmed publisher
	Oh M, Lee S, Lee G, Kim C, Kim E, Song J, et al. In vivo tracking of 14C thymidine labeled mesenchymal stem cells using ultra-sensitive accelerator mass spectrometry. Sci Rep. 2021;11:1360 pubmed publisher
	Kaczorowski A, Tolstov Y, Falkenstein M, Vasioukhin V, Prigge E, Geisler C, et al. Rearranged ERG confers robustness to prostate cancer cells by subverting the function of p53. Urol Oncol. 2020;38:736.e1-736.e10 pubmed publisher
	Xu Y, Gao Q, Zhang J, Lu Y, Xing D, Qin Y, et al. Prolyl hydroxylase 3 controls the intestine goblet cell generation through stabilizing ATOH1. Cell Death Differ. 2020;27:2131-2142 pubmed publisher
	Xu Y, Gao Q, Xue Y, Li X, Xu L, Li C, et al. Prolyl hydroxylase 3 stabilizes the p53 tumor suppressor by inhibiting the p53-MDM2 interaction in a hydroxylase-independent manner. J Biol Chem. 2019;294:9949-9958 pubmed publisher
	Breton Y, Desrosiers V, Ouellet M, Deshiere A, Torresilla C, Cohen E, et al. Expression of MDM2 in Macrophages Promotes the Early Postentry Steps of HIV-1 Infection through Inhibition of p53. J Virol. 2019;93: pubmed publisher
	Egners A, Rezaei M, Kuzmanov A, Poitz D, Streichert D, MULLER REICHERT T, et al. PHD3 Acts as Tumor Suppressor in Mouse Osteosarcoma and Influences Tumor Vascularization via PDGF-C Signaling. Cancers (Basel). 2018;10: pubmed publisher
	Chowdhury A, Aich A, Jain G, Wozny K, Lüchtenborg C, Hartmann M, et al. Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia. Cell Rep. 2018;25:561-570.e6 pubmed publisher
	Yano H, Sakai M, Matsukawa T, Yagi T, Naganuma T, Mitsushima M, et al. PHD3 regulates glucose metabolism by suppressing stress-induced signalling and optimising gluconeogenesis and insulin signalling in hepatocytes. Sci Rep. 2018;8:14290 pubmed publisher
	Sim J, Cowburn A, Palazon A, Madhu B, Tyrakis P, Macías D, et al. The Factor Inhibiting HIF Asparaginyl Hydroxylase Regulates Oxidative Metabolism and Accelerates Metabolic Adaptation to Hypoxia. Cell Metab. 2018;27:898-913.e7 pubmed publisher
	Ramos G, Cruz A, Silva W, Rozanski A, Baptista I, Silvestre J, et al. Thyroid hormone upregulates MDM2 in rat type i fiber: implications for skeletal muscle mass regulation. Acta Physiol (Oxf). 2017;: pubmed publisher
	Bialešová L, Xu L, Gustafsson J, Haldosén L, Zhao C, Dahlman Wright K. Estrogen receptor ?2 induces proliferation and invasiveness of triple negative breast cancer cells: association with regulation of PHD3 and HIF-1?. Oncotarget. 2017;8:76622-76633 pubmed publisher
	Gibier J, Hemon B, Fanchon M, Gaudelot K, Pottier N, Ringot B, et al. Dual role of MUC1 mucin in kidney ischemia-reperfusion injury: Nephroprotector in early phase, but pro-fibrotic in late phase. Biochim Biophys Acta Mol Basis Dis. 2017;1863:1336-1349 pubmed publisher
	Tan J, Prosser H, Dunn L, Vanags L, Ridiandries A, Tsatralis T, et al. High-Density Lipoproteins Rescue Diabetes-Impaired Angiogenesis via Scavenger Receptor Class B Type I. Diabetes. 2016;65:3091-103 pubmed publisher
	Shin J, Nunomiya A, Kitajima Y, Dan T, Miyata T, Nagatomi R. Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway. Skelet Muscle. 2016;6:5 pubmed publisher
	Heir P, Srikumar T, Bikopoulos G, Bunda S, Poon B, Lee J, et al. Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling. J Biol Chem. 2016;291:7357-72 pubmed publisher
	Zieseniss A, Hesse A, Jatho A, Krull S, Hölscher M, Vogel S, et al. Cardiomyocyte-Specific Transgenic Expression of Prolyl-4-Hydroxylase Domain 3 Impairs the Myocardial Response to Ischemia. Cell Physiol Biochem. 2015;36:843-51 pubmed publisher
	Pastor Soler N, Sutton T, Mang H, Kinlough C, Gendler S, Madsen C, et al. Muc1 is protective during kidney ischemia-reperfusion injury. Am J Physiol Renal Physiol. 2015;308:F1452-62 pubmed publisher
	Martin K, Trouche D, Hagemeier C, Sørensen T, La Thangue N, Kouzarides T. Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein. Nature. 1995;375:691-4 pubmed

product information

master code :

NB100-2736

SKU :

NB100-2736

product name :

MDM2/HDM2 Antibody (SMP14) - BSA Free

unit size :

0.1 ml (also 0.025 ml)

description :

The MDM2/HDM2 Antibody (SMP14) - BSA Free from Novus is a mouse monoclonal antibody to MDM2/HDM2. This antibody reacts with human,mouse,rat. The MDM2/HDM2 Antibody (SMP14) - BSA Free has been validated for the following applications: IF/IHC,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry.

target :

MDM2/HDM2

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

SMP14

concentration :

1 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProt# Q00987]

isotype :

IgG1 Kappa

purity :

Protein G purified

species :

Human,Mouse,Rat

specificity :

The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details

gene symbol :

MDM2

accessionNumbers :

Q00987

applications :

IF/IHC,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry

USD :

409 USD

alt names :

ACTFS, HDM2, HDMX, MDM2 oncogene, E3 ubiquitin protein ligase

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage