product summary
request information :
company name :
Novus Biologicals
product type :
antibody
product name :
Rabbit Polyclonal HIF-3 alpha Antibody
catalog :
NB100-2529G
quantity :
0.1 ml
price :
399.00 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human, mouse
application :
western blot, immunoprecipitation
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB100-2529G
Product Name :
Rabbit Polyclonal HIF-3 alpha Antibody
List Price :
399.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
Basic-helix-loop-helix-PAS protein MOP7, BHLHE17, bHLHe17HIF-3A2, Class E basic helix-loop-helix protein 17, HIF-3A4, HIF-3-alpha, HIF3-alpha, hypoxia-inducible factor 3-alpha, hypoxia-inducible factor-3 alpha 4, hypoxia inducible factor 3, alpha subunit, Inhibitory PAS domain protein, IPASHIF3-alpha-1, Member of PAS protein 7, MOP7PAS domain-containing protein 7, PASD7HIF-3A
Background :
Hypoxia-inducible factor (HIF) is one of the most important factors in the cellular response to hypoxia, transcriptionally activating genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits, HIF1 alpha, HIF2 alpha, or HIF3 alpha. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation and cell survival. HIF3 alpha protein is one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha 3 subunit lacks the transactivation domain found in factors containing either the alpha 1 or alpha 2 subunits. HIF3 alpha may be a marker for tumor growth and angiogenesis.
Buffer :
50mM sodium borate
Clonality :
Polyclonal
Concentration :
24.0 mg/ml
Conjugate :
DyLight 488
Application Summary :
Western Blot, Immunoprecipitation
Host :
Rabbit
Immunogen :
A synthetic peptide made to an internal region of human HIF-3 alpha (within residues 250-350). [Swiss-Prot# Q66K72]
Preservative :
No Preservative
Purity :
IgG purified
CrossReactivity :
Human and mouse.
Storage :
Store at 4C in the dark.
Swissprot Human :
Q9Y2N7
TMW :
68 kDa
Uses :
This HIF-3 alpha antibody is useful for Immunoprecipitation, Western Blot and Immunocytochemistry/Immunofluorescence. In Western Blot analysis, a band is recognized at ~68 kDa representing HIF-3 alpha. Because a band at ~71 kDa is also seen in hypoxic samples,it is important to run a negative control lysate as well (see image). In ICC/IF, cytoplasmic and nuclear staining was observed in HeLa cells, which is consistent with the literature under normoxic conditions for HIF-3 alpha (PMID: 16775626).
Research Area :
Angiogenesis, Cancer, Chromatin Research, HIF Target Genes, Hypoxia, Transcription Factors and Regulators
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: usa
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.