product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rabbit Polyclonal AlphaB Crystallin/CRYAB Antibody
catalog :
NB100-2520F
quantity :
0.1 ml
price :
299.00 USD
clonality :
polyclonal
host :
rabbit
conjugate :
FITC
reactivity :
human, rat, cow
application :
western blot
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB100-2520F
Product Name :
Rabbit Polyclonal AlphaB Crystallin/CRYAB Antibody
List Price :
299.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
CRYA2alpha crystallin B chain, crystallin, alpha B, CTPP2, Heat shock protein beta-5, heat-shock 20 kD like-protein, HSPB5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, alpha B crystallin, alpha(B)-crystallin, Alpha(B)-crystallin, alpha-crystallin B chain
Background :
Crystallin AB (CRYAB or alpha-basic-crystallin) belongs to HSP20 family and was originally discovered as chaperone abundantly expressed in eye lens where it plays a key role in maintaining transparency and refractive index of lens. Subsequently, CRYAB was found to acts as a molecular chaperone induced by a variety of stress stimuli, conferring cytoprotection by suppressing aggregation of denatured protein. In its anti-apoptotic role, CRYAB protects cells against stress, TNF-alpha, apoptotic effectors etc. and can inhibit caspase-3/PARP alongwith preventing Bax/Bcl-2 translocation. CRYAB is a heteropolymer of three CRYAA and one CRYAB subunits, and can aggregate with homologous proteins (e.g. HSPB1) to form large complexes and interact with HSPBAP1 as well as TTN/titin. Localized in cytoplasm, during heat shock stimulus, CRYAB translocates to sub-nuclear structures known as SC35/nuclear splicing speckles. CRYAB is expressed in several other tissues such as muscle, brain, spleen, lung, kidney, skin etc and has been shown to implicate in certain diseases including cardiac myopathies, neurodegenerative diseases and malignancies including brain, breast, prostate and renal cancers. Defectsive CRYAB causes myopathy myofibrillar type 2 (MFM2), cataract posterior polar type 2 (CTPP2) and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).
Buffer :
50mM sodium borate
Clonality :
Polyclonal
Conjugate :
FITC
Application Summary :
Western Blot
Host :
Rabbit
Immunogen :
A synthetic peptide made to a C-terminal region of the human Crystallin AB protein (within residues 100-175). [Swiss-Prot P02511]
Preservative :
No Preservative
Purity :
Immunogen affinity purified
CrossReactivity :
Human. Predicted to react with rat, sheep, rabbit, monkey, hamster, cow, and pig based on 100% sequence homology. Immunogen sequence has 92% homology to mouse.
Storage :
Store at 4C in the dark.
Swissprot Human :
P02511
Uses :
This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. **CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes.
Research Area :
Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience, Neurotransmission, Phospho Specific, Protein Phosphatase, Sensory Systems, Signal Transduction, Tyrosine Kinases, Vision
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.