product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rabbit Polyclonal HP1 alpha Antibody
catalog :
NB100-2518H
quantity :
0.1 ml
price :
319.00 USD
clonality :
polyclonal
host :
rabbit
conjugate :
HRP
reactivity :
human, mouse
application :
western blot
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB100-2518H
Product Name :
Rabbit Polyclonal HP1 alpha Antibody
List Price :
319.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
chromobox homolog 5, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha, HP1, HP1 alpha, HP1 alpha homolog, HP1A, HP1-ALPHA, HP1Hs alpha, HP1Hs-alpha, Antigen p25
Background :
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase. A molecular mechanism through which the SUV39H-HP1 methylation system can contribute to the propagation of heterochromatic subdomains in native chromatin has been defined. It has been demonstrated that HP1 can bind with high affinity to histone H3 methylated at lysine-9 but not at lysine-4. The chromodomain of HP1 as its methyl-lysine-binding domain has been identified. A point mutation in the chromodomain, which destroys the gene silencing activity of HP1 in Drosophila, abolished methyl-lysine-binding activity. Genetic and biochemical analysis in S. pombe showed that the methylase activity of Clr4 (the SUV39H1 homolog) is necessary for the correct localization of Swi6 (the HP1 equivalent) at centromeric heterochromatin and for gene silencing. A stepwise model for the formation of a transcriptionally silent heterochromatin: SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromodomain has been suggested. This model may also explain the stable inheritance of the heterochromatic state. SUV39H1 and HP1 are both involved in the repressive functions of the retinoblastoma protein. Rb associates with SUV39H1 and HP1 in vivo by means of its pocket domain. SUV39H1 cooperates with Rb to repress the cyclin E promoter, and in fibroblasts that are disrupted for SUV39H1, the activity of the cyclin E and cyclin A2 genes are specifically elevated. The SUV39H1-HP1 complex is not only involved in heterochromatic silencing but also has a role in repression of euchromatic genes by Rb and perhaps other corepressor proteins.
Buffer :
PBS
Clonality :
Polyclonal
Conjugate :
HRP
Application Summary :
Western Blot
Host :
Rabbit
Immunogen :
A synthetic peptide containing an N-terminal region of human HP1 alpha (within residues 1-100). [Swiss-Prot P45973]
Localization :
Nuclear
Marker :
Heterochromatin Marker
Preservative :
No Preservative
Purity :
IgG purified
CrossReactivity :
Human. Expected to react with mouse, Arabidopsis suecica, Rye and Triticum aestivum due to sequence homology.
Storage :
Store at 4C in the dark.
Swissprot Human :
P45973
TMW :
20 kDa
Uses :
This HP1 alpha antibody is useful for Western blot analysis, where a band is seen at ~20 kDa.
Research Area :
Cellular Markers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.