product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
LC3B Antibody - BSA Free
catalog :
NB100-2220
quantity :
0.1 ml (also 0.025 ml)
price :
459 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
guinea pig, human, mouse, rat, dogs, chicken, bovine, zebrafish
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section, proximity ligation assay
more info or order :
citations: 1112
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 4a, 4b
Moore T, Cheng L, Wolf D, Ngo J, Segawa M, Zhu X, et al. Parkin regulates adiposity by coordinating mitophagy with mitochondrial biogenesis in white adipocytes. Nat Commun. 2022;13:6661 pubmed publisher
  • western blot; human; loading ...; fig 1d
  • western blot; mouse; loading ...; fig 3d, 8b
Yang C, Su C, Iyaswamy A, Krishnamoorthi S, Zhu Z, Yang S, et al. Celastrol enhances transcription factor EB (TFEB)-mediated autophagy and mitigates Tau pathology: Implications for Alzheimer's disease therapy. Acta Pharm Sin B. 2022;12:1707-1722 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 2e
  • western blot; human; 1:500; loading ...; fig 4d
Li M, Shen Y, Xiong Y, Wang S, Li C, Bai J, et al. Loss of SMARCB1 promotes autophagy and facilitates tumour progression in chordoma by transcriptionally activating ATG5. Cell Prolif. 2021;54:e13136 pubmed publisher
  • western blot; human; fig 4b
  • western blot; rat; loading ...; fig 1d
Shi Y, Hu Y, Wang Y, Ma X, Tang L, Tao M, et al. Blockade of Autophagy Prevents the Development and Progression of Peritoneal Fibrosis. Front Pharmacol. 2021;12:724141 pubmed publisher
  • western blot; mouse; loading ...
Silva Rojas R, Charles A, Djeddi S, Geny B, Laporte J, Böhm J. Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure. Cells. 2021;10: pubmed publisher
  • western blot; mouse; loading ...; fig 1g
Yoon Y, Go G, Yoon S, Lim J, Lee G, Lee J, et al. Melatonin Treatment Improves Renal Fibrosis via miR-4516/SIAH3/PINK1 Axis. Cells. 2021;10: pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 2c
Liu H, Zang P, Lee I, Anderson B, Christiani A, Strait Bodey L, et al. Growth hormone secretagogue receptor-1a mediates ghrelin's effects on attenuating tumour-induced loss of muscle strength but not muscle mass. J Cachexia Sarcopenia Muscle. 2021;12:1280-1295 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6a
Amegandjin C, Choudhury M, Jadhav V, Carriço J, Quintal A, Berryer M, et al. Sensitive period for rescuing parvalbumin interneurons connectivity and social behavior deficits caused by TSC1 loss. Nat Commun. 2021;12:3653 pubmed publisher
  • western blot; human; loading ...; fig 1d
Poon A, Saini H, Sethi S, O Sullivan G, Plun Favreau H, Wray S, et al. The role of SQSTM1 (p62) in mitochondrial function and clearance in human cortical neurons. Stem Cell Reports. 2021;16:1276-1289 pubmed publisher
  • western blot; mouse; 1:3000; loading ...; fig 2i
Choi G, Lee H, Chae C, Cho J, Jung Y, Kim J, et al. BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects. Nat Commun. 2021;12:487 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1l
Stojakovic A, Trushin S, Sheu A, Khalili L, Chang S, Li X, et al. Partial inhibition of mitochondrial complex I ameliorates Alzheimer's disease pathology and cognition in APP/PS1 female mice. Commun Biol. 2021;4:61 pubmed publisher
  • western blot; human; 1:2000; loading ...
Dong X, Yang Y, Zou Z, Zhao Y, Ci B, Zhong L, et al. Sorting nexin 5 mediates virus-induced autophagy and immunity. Nature. 2021;589:456-461 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 2e
  • western blot; mouse; 1:5000; loading ...; fig 2a
Brattås P, Hersbach B, Madsen S, Petri R, Jakobsson J, Pircs K. Impact of differential and time-dependent autophagy activation on therapeutic efficacy in a model of Huntington disease. Autophagy. 2021;17:1316-1329 pubmed publisher
  • western blot; human; loading ...; fig 1a
Tiwari S, Dang J, Lin N, Qin Y, Wang S, Rana T. Zika virus depletes neural stem cells and evades selective autophagy by suppressing the Fanconi anemia protein FANCC. EMBO Rep. 2020;:e49183 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 1c
Ogasawara Y, Cheng J, Tatematsu T, Uchida M, Murase O, Yoshikawa S, et al. Long-term autophagy is sustained by activation of CCTβ3 on lipid droplets. Nat Commun. 2020;11:4480 pubmed publisher
  • western blot; mouse; loading ...; fig s3a
Li Z, Zhang H, Huang Y, Huang J, Sun P, Zhou N, et al. Autophagy deficiency promotes triple-negative breast cancer resistance to T cell-mediated cytotoxicity by blocking tenascin-C degradation. Nat Commun. 2020;11:3806 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4a
Lanzillotta C, Zuliani I, Vasavda C, Snyder S, Paul B, Perluigi M, et al. BVR-A Deficiency Leads to Autophagy Impairment through the Dysregulation of AMPK/mTOR Axis in the Brain-Implications for Neurodegeneration. Antioxidants (Basel). 2020;9: pubmed publisher
  • western blot; human; loading ...; fig 4a
Zhuang X, Wang S, Tan Y, Song J, Zhu Z, Wang Z, et al. Pharmacological enhancement of TFEB-mediated autophagy alleviated neuronal death in oxidative stress-induced Parkinson's disease models. Cell Death Dis. 2020;11:128 pubmed publisher
  • western blot; human; loading ...; fig 2a
Jo D, Park S, Kim A, Park N, Kim J, Bae J, et al. Loss of HSPA9 induces peroxisomal degradation by increasing pexophagy. Autophagy. 2020;:1-15 pubmed publisher
  • western blot; mouse; loading ...; fig 2a
Tang C, Han H, Liu Z, Liu Y, Yin L, Cai J, et al. Activation of BNIP3-mediated mitophagy protects against renal ischemia-reperfusion injury. Cell Death Dis. 2019;10:677 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s2a
  • western blot; human; loading ...; fig 2a
Yang M, Chen P, Liu J, Zhu S, Kroemer G, Klionsky D, et al. Clockophagy is a novel selective autophagy process favoring ferroptosis. Sci Adv. 2019;5:eaaw2238 pubmed publisher
  • western blot; mouse; 1:20,000; loading ...; fig 6h
Jung S, Choe S, Woo H, Jeong H, An H, Moon H, et al. Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits. Autophagy. 2019;:1-19 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1c
Li L, Kang H, Zhang Q, D Agati V, Al Awqati Q, Lin F. FoxO3 activation in hypoxic tubules prevents chronic kidney disease. J Clin Invest. 2019;129:2374-2389 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 2a
Li Z, Tian Y, Qu L, Mao J, Zhong H. AAV-Mig-6 Increase the Efficacy of TAE in VX2 Rabbit Model, Is Associated With JNK Mediated Autophagy. J Cancer. 2019;10:1060-1069 pubmed publisher
  • western blot; human; fig 3g
Park H, Chung K, An H, Gim J, Hong J, Woo H, et al. Parkin Promotes Mitophagic Cell Death in Adult Hippocampal Neural Stem Cells Following Insulin Withdrawal. Front Mol Neurosci. 2019;12:46 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 8c
Carballo Carbajal I, Laguna A, Romero Gimenez J, Cuadros T, Bove J, Martinez Vicente M, et al. Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson's disease pathogenesis. Nat Commun. 2019;10:973 pubmed publisher
  • western blot; human; loading ...; fig 5a
Huang X, Gan G, Wang X, Xu T, Xie W. The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance. Autophagy. 2019;15:1258-1279 pubmed publisher
  • western blot; guinea pig; loading ...; fig 7b
Zhang Y, Jiang Q, Xie S, Wu X, Zhou J, Sun H. Lead Induced Ototoxicity and Neurotoxicity in Adult Guinea Pig. Biomed Res Int. 2019;2019:3626032 pubmed publisher
  • western blot; human; loading ...; fig 1a
Wang W, Xia Z, Farre J, Subramani S. TRIM37 deficiency induces autophagy through deregulating the MTORC1-TFEB axis. Autophagy. 2018;14:1574-1585 pubmed publisher
  • western blot; human; loading ...; fig 3c
Marrone L, Bus C, Schöndorf D, Fitzgerald J, Kübler M, Schmid B, et al. Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease. PLoS ONE. 2018;13:e0192497 pubmed publisher
  • western blot; human; 1:3000; loading ...; fig 1b
Hsu C, Lee E, Gordon K, Paz E, Shen W, Ohnishi K, et al. MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB. Nat Commun. 2018;9:942 pubmed publisher
  • western blot; human; loading ...; fig 2a
Agod Z, Pazmandi K, Bencze D, Vereb G, Biro T, Szabo A, et al. Signaling Lymphocyte Activation Molecule Family 5 Enhances Autophagy and Fine-Tunes Cytokine Response in Monocyte-Derived Dendritic Cells via Stabilization of Interferon Regulatory Factor 8. Front Immunol. 2018;9:62 pubmed publisher
  • western blot; human; loading ...; fig 6a
Goiran T, Duplan E, Rouland L, El Manaa W, Lauritzen I, Dunys J, et al. Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation. Cell Death Differ. 2018;25:873-884 pubmed publisher
  • western blot; mouse; 1:3000; loading ...; fig 4b
Sun H, Krauss R, Chang J, Teng B. PCSK9 deficiency reduces atherosclerosis, apolipoprotein B secretion, and endothelial dysfunction. J Lipid Res. 2018;59:207-223 pubmed publisher
  • western blot; mouse; 1:2000; fig 5g
Lüningschrör P, Binotti B, Dombert B, Heimann P, Pérez Lara A, Slotta C, et al. Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease. Nat Commun. 2017;8:678 pubmed publisher
  • western blot; mouse; loading ...; fig 2f
Bartolomeo R, Cinque L, De Leonibus C, Forrester A, Salzano A, Monfregola J, et al. mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest. 2017;127:3717-3729 pubmed publisher
  • western blot; mouse; loading ...; fig 2c
Rocchi A, Yamamoto S, Ting T, Fan Y, SADLEIR K, Wang Y, et al. A Becn1 mutation mediates hyperactive autophagic sequestration of amyloid oligomers and improved cognition in Alzheimer's disease. PLoS Genet. 2017;13:e1006962 pubmed publisher
  • western blot; human; 1:10,000; loading ...; fig 1f
Button R, Roberts S, Willis T, Hanemann C, Luo S. Accumulation of autophagosomes confers cytotoxicity. J Biol Chem. 2017;292:13599-13614 pubmed publisher
  • western blot; mouse; loading ...; fig 2c
Wu D, Adamopoulos I. Loss of WDFY3 ameliorates severity of serum transfer-induced arthritis independently of autophagy. Cell Immunol. 2017;316:61-69 pubmed publisher
  • western blot; mouse; loading ...; fig 1a
  • western blot; human; loading ...; fig e4d
Ashkenazi A, Bento C, Ricketts T, Vicinanza M, Siddiqi F, Pavel M, et al. Polyglutamine tracts regulate beclin 1-dependent autophagy. Nature. 2017;545:108-111 pubmed publisher
  • western blot; human; loading ...; fig 5b
  • western blot; zebrafish ; fig 3d
Zhang Y, Nguyen D, Olzomer E, Poon G, Cole N, Puvanendran A, et al. Rescue of Pink1 Deficiency by Stress-Dependent Activation of Autophagy. Cell Chem Biol. 2017;24:471-480.e4 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4d
Delaney J, Patel C, Willis K, Haghighiabyaneh M, Axelrod J, Tancioni I, et al. Haploinsufficiency networks identify targetable patterns of allelic deficiency in low mutation ovarian cancer. Nat Commun. 2017;8:14423 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 8c
Granato M, Rizzello C, Gilardini Montani M, Cuomo L, Vitillo M, Santarelli R, et al. Quercetin induces apoptosis and autophagy in primary effusion lymphoma cells by inhibiting PI3K/AKT/mTOR and STAT3 signaling pathways. J Nutr Biochem. 2017;41:124-136 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 5a
Hosoya M, Fujioka M, Sone T, Okamoto S, Akamatsu W, Ukai H, et al. Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss. Cell Rep. 2017;18:68-81 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 1b
Wei Y, Chiang W, Sumpter R, Mishra P, Levine B. Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor. Cell. 2017;168:224-238.e10 pubmed publisher
  • western blot; human; 1:3000; loading ...; fig 3a
Pavel M, Imarisio S, Menzies F, Jimenez Sanchez M, Siddiqi F, Wu X, et al. CCT complex restricts neuropathogenic protein aggregation via autophagy. Nat Commun. 2016;7:13821 pubmed publisher
  • western blot; mouse; fig 3d
Su F, Myers V, Knezevic T, Wang J, Gao E, Madesh M, et al. Bcl-2-associated athanogene 3 protects the heart from ischemia/reperfusion injury. JCI Insight. 2016;1:e90931 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1f
Lee M, Sumpter R, Zou Z, Sirasanagandla S, Wei Y, Mishra P, et al. Peroxisomal protein PEX13 functions in selective autophagy. EMBO Rep. 2017;18:48-60 pubmed publisher
  • western blot; human; loading ...; fig 1h
Li D, Xie B, Wu X, Li J, Ding Y, Wen X, et al. Late-stage inhibition of autophagy enhances calreticulin surface exposure. Oncotarget. 2016;7:80842-80854 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 5a
Cai Y, Yang L, Hu G, Chen X, Niu F, Yuan L, et al. Regulation of morphine-induced synaptic alterations: Role of oxidative stress, ER stress, and autophagy. J Cell Biol. 2016;215:245-258 pubmed
  • western blot; mouse; loading ...; fig 3f
Fan Y, Wang N, Rocchi A, Zhang W, Vassar R, Zhou Y, et al. Identification of natural products with neuronal and metabolic benefits through autophagy induction. Autophagy. 2017;13:41-56 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 5a
White S, McDermott M, Sufit R, Kosmac K, Bugg A, Gonzalez Freire M, et al. Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study. J Transl Med. 2016;14:284 pubmed publisher
  • western blot; human; loading ...; fig 5b
Teo W, Kerr M, Teasdale R. MTMR4 Is Required for the Stability of the Salmonella-Containing Vacuole. Front Cell Infect Microbiol. 2016;6:91 pubmed publisher
  • western blot; rat; 1:1000; loading ...
Cudré Cung H, Zavadakova P, Do Vale Pereira S, Remacle N, Henry H, Ivanisevic J, et al. Ammonium accumulation is a primary effect of 2-methylcitrate exposure in an in vitro model for brain damage in methylmalonic aciduria. Mol Genet Metab. 2016;119:57-67 pubmed publisher
  • western blot; human; loading ...; fig 4e
Lin M, Liu H, Xiong Q, Niu H, Cheng Z, Yamamoto A, et al. Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase. Autophagy. 2016;12:2145-2166 pubmed
  • western blot; human; fig 1e
Jo Y, Park N, Park S, Kim B, Shin J, Jo D, et al. O-GlcNAcylation of ATG4B positively regulates autophagy by increasing its hydroxylase activity. Oncotarget. 2016;7:57186-57196 pubmed publisher
  • western blot; mouse; 1:5000; fig 6
Zea A, Stewart T, Ascani J, Tate D, Finkel Jimenez B, Wilk A, et al. Activation of the IL-2 Receptor in Podocytes: A Potential Mechanism for Podocyte Injury in Idiopathic Nephrotic Syndrome?. PLoS ONE. 2016;11:e0157907 pubmed publisher
  • western blot; human; fig 3
Dejesus R, Moretti F, McAllister G, Wang Z, Bergman P, Liu S, et al. Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62. elife. 2016;5: pubmed publisher
  • immunocytochemistry; human; fig 3
  • western blot; human; 1:1000; fig 3
Davis M, Delaney J, Patel C, Storgard R, Stupack D. Nelfinavir is effective against human cervical cancer cells in vivo: a potential treatment modality in resource-limited settings. Drug Des Devel Ther. 2016;10:1837-46 pubmed publisher
  • western blot; human
Kuramoto K, Wang N, Fan Y, Zhang W, Schoenen F, Frankowski K, et al. Autophagy activation by novel inducers prevents BECN2-mediated drug tolerance to cannabinoids. Autophagy. 2016;12:1460-71 pubmed publisher
  • immunocytochemistry; human; 1:400; loading ...; fig 2d
  • western blot; human; 1:1000; loading ...; fig 5b
Wijdeven R, Janssen H, Nahidiazar L, Janssen L, Jalink K, Berlin I, et al. Cholesterol and ORP1L-mediated ER contact sites control autophagosome transport and fusion with the endocytic pathway. Nat Commun. 2016;7:11808 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1a
Barnard R, Regan D, Hansen R, Maycotte P, Thorburn A, Gustafson D. Autophagy Inhibition Delays Early but Not Late-Stage Metastatic Disease. J Pharmacol Exp Ther. 2016;358:282-93 pubmed publisher
  • western blot; human; loading ...; fig 4b
Park S, Yi H, Suh N, Park Y, Koh J, Jeong S, et al. Inhibition of EHMT2/G9a epigenetically increases the transcription of Beclin-1 via an increase in ROS and activation of NF-?B. Oncotarget. 2016;7:39796-39808 pubmed publisher
  • western blot; rat; fig 10
  • immunocytochemistry; mouse; fig 7
  • western blot; mouse; fig 1
Song J, Sun Y, Peluso I, Zeng Y, Yu X, Lu J, et al. A novel curcumin analog binds to and activates TFEB in vitro and in vivo independent of MTOR inhibition. Autophagy. 2016;12:1372-89 pubmed publisher
  • western blot; mouse; fig 6
Pastore N, Brady O, Diab H, Martina J, Sun L, Huynh T, et al. TFEB and TFE3 cooperate in the regulation of the innate immune response in activated macrophages. Autophagy. 2016;12:1240-58 pubmed publisher
  • western blot; human; fig 6
Lopes V, Loitto V, Audinot J, Bayat N, Gutleb A, Cristobal S. Dose-dependent autophagic effect of titanium dioxide nanoparticles in human HaCaT cells at non-cytotoxic levels. J Nanobiotechnology. 2016;14:22 pubmed publisher
  • western blot; zebrafish ; 1:2000; fig s2
Ruparelia A, Oorschot V, Ramm G, Bryson Richardson R. FLNC myofibrillar myopathy results from impaired autophagy and protein insufficiency. Hum Mol Genet. 2016;25:2131-2142 pubmed
  • western blot; mouse; loading ...
Liu Y, Takahashi Y, Desai N, Zhang J, Serfass J, Shi Y, et al. Bif-1 deficiency impairs lipid homeostasis and causes obesity accompanied by insulin resistance. Sci Rep. 2016;6:20453 pubmed publisher
  • western blot; human; 1:1000; fig s1
  • western blot; mouse; 1:1000; fig 6
Wu X, Fleming A, Ricketts T, Pavel M, Virgin H, Menzies F, et al. Autophagy regulates Notch degradation and modulates stem cell development and neurogenesis. Nat Commun. 2016;7:10533 pubmed publisher
  • western blot; human; fig 3
Gentry E, Henderson B, Arrant A, Gearing M, Feng Y, Riddle N, et al. Rho Kinase Inhibition as a Therapeutic for Progressive Supranuclear Palsy and Corticobasal Degeneration. J Neurosci. 2016;36:1316-23 pubmed publisher
  • western blot; human; 1:10,000; fig 1
Button R, Vincent J, Strang C, Luo S. Dual PI-3 kinase/mTOR inhibition impairs autophagy flux and induces cell death independent of apoptosis and necroptosis. Oncotarget. 2016;7:5157-75 pubmed publisher
  • western blot; human; 1:2000; fig 3
Mukherjee R, Chakrabarti O. Ubiquitin-mediated regulation of the E3 ligase GP78 by MGRN1 in trans affects mitochondrial homeostasis. J Cell Sci. 2016;129:757-73 pubmed publisher
  • western blot; human; 1:5000; fig 7
Kraft L, Manral P, Dowler J, Kenworthy A. Nuclear LC3 Associates with Slowly Diffusing Complexes that Survey the Nucleolus. Traffic. 2016;17:369-99 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 2e
Stotland A, Gottlieb R. α-MHC MitoTimer mouse: In vivo mitochondrial turnover model reveals remarkable mitochondrial heterogeneity in the heart. J Mol Cell Cardiol. 2016;90:53-8 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig s4b
Sorrell S, Golder Z, Johnstone D, Frankl F. Renal peroxiredoxin 6 interacts with anion exchanger 1 and plays a novel role in pH homeostasis. Kidney Int. 2016;89:105-112 pubmed publisher
  • western blot; human; fig 2
Hermanova I, Arruabarrena Aristorena A, Valis K, Nůsková H, Alberich Jorda M, Fiser K, et al. Pharmacological inhibition of fatty-acid oxidation synergistically enhances the effect of l-asparaginase in childhood ALL cells. Leukemia. 2016;30:209-18 pubmed publisher
  • western blot; rat; fig 5
Chesser A, Ganeshan V, Yang J, Johnson G. Epigallocatechin-3-gallate enhances clearance of phosphorylated tau in primary neurons. Nutr Neurosci. 2016;19:21-31 pubmed publisher
  • western blot; human
Hsuan S, Chyau C, Hung H, Chen J, Chou F. The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer. Eur J Nutr. 2016;55:491-503 pubmed publisher
  • western blot; mouse
Xu Y, Tian C, Sun J, Zhang J, Ren K, Fan X, et al. FBXW7-Induced MTOR Degradation Forces Autophagy to Counteract Persistent Prion Infection. Mol Neurobiol. 2016;53:706-719 pubmed publisher
Keary K, Gu Q, Chen J, Li Z. Dendritic distribution of autophagosomes underlies pathway-selective induction of LTD. Cell Rep. 2023;42:112898 pubmed publisher
Anugula S, Li Z, Li Y, Hendriksen A, Christensen P, Wang L, et al. Rev1 deficiency induces a metabolic shift in MEFs that can be manipulated by the NAD+ precursor nicotinamide riboside. Heliyon. 2023;9:e17392 pubmed publisher
Vo M, Choi C, Choi Y. The mitophagy receptor NIX induces vIRF-1 oligomerization and interaction with GABARAPL1 for the promotion of HHV-8 reactivation-induced mitophagy. PLoS Pathog. 2023;19:e1011548 pubmed publisher
Boutry M, DiGiovanni L, Demers N, Fountain A, Mamand S, Botelho R, et al. Arf1-PI4KIIIβ positive vesicles regulate PI(3)P signaling to facilitate lysosomal tubule fission. J Cell Biol. 2023;222: pubmed publisher
Hou X, Chen T, Koga S, Bredenberg J, Faroqi A, Delenclos M, et al. Alpha-synuclein-associated changes in PINK1-PRKN-mediated mitophagy are disease context dependent. Brain Pathol. 2023;:e13175 pubmed publisher
Kim Y, Seong C, Cho K, Lee S, Kim T, Kim H, et al. Anti‑tumor properties of FoxO1 in YD‑9 oral squamous cell carcinoma cells. Oncol Rep. 2023;49: pubmed publisher
Shahin S, Lu B, Zhou Y, Xu H, Chetsawang J, Baloh R, et al. MFN1 augmentation prevents retinal degeneration in a Charcot-Marie-Tooth type 2A mouse model. iScience. 2023;26:106270 pubmed publisher
Bi Y, Yang G, Guo Z, Cai W, Chen S, Zhou X, et al. Chronic high‑salt intake induces cardiomyocyte autophagic vacuolization during left ventricular maladaptive remodeling in spontaneously hypertensive rats. Exp Ther Med. 2023;25:148 pubmed publisher
Bensalem J, Hein L, Hassiotis S, Trim P, Proud C, Heilbronn L, et al. Modifying Dietary Protein Impacts mTOR Signaling and Brain Deposition of Amyloid β in a Knock-In Mouse Model of Alzheimer Disease. J Nutr. 2023;153:1407-1419 pubmed publisher
Kim S, Cho Y, Kim Y, Park J, Yoo S, Gwak J, et al. Endolysosomal impairment by binding of amyloid beta or MAPT/Tau to V-ATPase and rescue via the HYAL-CD44 axis in Alzheimer disease. Autophagy. 2023;:1-20 pubmed publisher
Seliger C, Rauer L, W xfc ster A, Moeckel S, Leidgens V, Jachnik B, et al. Heterogeneity of Amino Acid Profiles of Proneural and Mesenchymal Brain-Tumor Initiating Cells. Int J Mol Sci. 2023;24: pubmed publisher
Wang Y, Pan C, Zhang X, Zhao A, Dong Y. Mutant NPM1 maintains RASGRP3 protein stability via interaction with MID1 to promote acute myeloid leukemia cell proliferation and autophagy. J Leukoc Biol. 2023;113:504-517 pubmed publisher
Charrin E, Dabaghie D, Sen I, Unnersj xf6 Jess D, M xf6 ller Hackbarth K, Burmakin M, et al. Soluble Klotho protects against glomerular injury through regulation of ER stress response. Commun Biol. 2023;6:208 pubmed publisher
Kim M, Choi G, Chae C, Lim J, Jung Y, Yoon J, et al. Melatonin-mediated FKBP4 downregulation protects against stress-induced neuronal mitochondria dysfunctions by blocking nuclear translocation of GR. Cell Death Dis. 2023;14:146 pubmed publisher
Xia Q, Li Y, Xu W, Wu C, Zheng H, Liu L, et al. Enhanced liquidity of p62 droplets mediated by Smurf1 links Nrf2 activation and autophagy. Cell Biosci. 2023;13:37 pubmed publisher
Wen H, Zuo Y, Li L, Zhan L, Xue J, Sun W, et al. Hypoxic postconditioning restores mitophagy against transient global cerebral ischemia via Parkin-induced posttranslational modification of TBK1. Neurobiol Dis. 2023;179:106043 pubmed publisher
Yu J, Liu T, Guo Q, Wang Z, Chen Y, Dong Y. Disruption of the Intestinal Mucosal Barrier Induced by High Fructose and Restraint Stress Is Regulated by the Intestinal Microbiota and Microbiota Metabolites. Microbiol Spectr. 2023;11:e0469822 pubmed publisher
Shen W, Yuh C, Lu Y, Lin Y, Ching T, Wang C, et al. Reduced Ribose-5-Phosphate Isomerase A-1 Expression in Specific Neurons and Time Points Promotes Longevity in Caenorhabditis elegans. Antioxidants (Basel). 2023;12: pubmed publisher
Doganlar O, Doganlar Z, Erdogan S, Delen E. Antineoplastic multi-drug chemotherapy to sensitize tumors triggers multi-drug resistance and inhibits efficiency of maintenance treatment inglioblastoma cells. EXCLI J. 2023;22:35-52 pubmed publisher
Higgins C, Adams J, Ward M, Greenberg Z, Milewska M, Sun J, et al. The tetraspanin transmembrane protein CD53 mediates dyslipidemia and integrates inflammatory and metabolic signaling in hepatocytes. J Biol Chem. 2023;299:102835 pubmed publisher
Hara K, Horikoshi Y, Morimoto M, Nakaso K, Sunaguchi T, Kurashiki T, et al. TYRO3 promotes chemoresistance via increased LC3 expression in pancreatic cancer. Transl Oncol. 2023;28:101608 pubmed publisher
Yu X, Xu X, Zhang S. Low-dose dexamethasone promotes osteoblast viability by activating autophagy via the SGK1/FOXO3a signaling pathway. Cell Biol Int. 2023;47:669-678 pubmed publisher
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Medina D, Di Paola S, Peluso I, Armani A, De Stefani D, Venditti R, et al. Lysosomal calcium signalling regulates autophagy through calcineurin and ​TFEB. Nat Cell Biol. 2015;17:288-99 pubmed
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Ulbricht A, Gehlert S, Leciejewski B, Schiffer T, Bloch W, Höhfeld J. Induction and adaptation of chaperone-assisted selective autophagy CASA in response to resistance exercise in human skeletal muscle. Autophagy. 2015;11:538-46 pubmed publisher
Sanjurjo L, Amézaga N, Aran G, Naranjo Gómez M, Arias L, Armengol C, et al. The human CD5L/AIM-CD36 axis: A novel autophagy inducer in macrophages that modulates inflammatory responses. Autophagy. 2015;11:487-502 pubmed publisher
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Kommaddi R, Jean Charles P, Shenoy S. Phosphorylation of the deubiquitinase USP20 by protein kinase A regulates post-endocytic trafficking of β2 adrenergic receptors to autophagosomes during physiological stress. J Biol Chem. 2015;290:8888-903 pubmed publisher
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Lin H. In Vitro and in Vivo Atheroprotective Effects of Gossypetin against Endothelial Cell Injury by Induction of Autophagy. Chem Res Toxicol. 2015;28:202-15 pubmed
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product information
brand :
Novus
MasterCode :
NB100-2220
SKU :
NB100-2220
product name :
LC3B Antibody - BSA Free
Description :
The LC3B Antibody - BSA Free from NB100-2220 is a nb100-2220 antibody to . This antibody reacts with autophagy, cancer, cardiovascular biology, cellular markers, hypoxia, innate immunity, lipid and metabolism, macroautophagy, neuroscience.
target :
LC3B
category :
Primary Antibodies
unit size :
0.1 ml (also 0.025 ml)
buffer :
PBS
clonality :
Polyclonal
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Rabbit
immunogen :
Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8]
isotype :
IgG
purity :
Immunogen affinity purified
species :
Human, Mouse, Rat, Porcine, Alligator, Avian, Bacteria, Bovine, Canine, Chicken, Chinese Hamster, Guinea Pig, Hamster, Invertebrate, Monkey, Primate, Rabbit, Golden Syrian Hamster, Zebrafish
theoretical molecular weight :
14.688 kDa
gene symbol :
MAP1LC3B
images :
https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Knockout-Validated-NB100-2220-img0045.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Knockout-Validated-NB100-2220-img0045.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Knockout-Validated-NB100-2220-img0045.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Knockout-Validated-NB100-2220-img0045.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Knockout-Validated-NB100-2220-img0059.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Knockout-Validated-NB100-2220-img0059.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Knockout-Validated-NB100-2220-img0059.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Knockout-Validated-NB100-2220-img0059.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0073.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0073.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0073.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0073.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0074.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0074.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0074.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0074.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Frozen-NB100-2220-img0051.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Frozen-NB100-2220-img0051.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Frozen-NB100-2220-img0051.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Frozen-NB100-2220-img0051.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0066.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0066.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0066.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0066.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0047.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0047.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0047.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0047.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0072.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0072.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0072.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0072.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0031.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0031.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0031.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0031.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0056.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0056.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0056.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0056.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0061.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0061.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0061.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0061.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0067.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0067.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0067.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0067.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0071.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0071.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0071.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Western-Blot-NB100-2220-img0071.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0064.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0064.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0064.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0064.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0065.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0065.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0065.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0065.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0037.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0037.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0037.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0037.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Paraffin-NB100-2220-img0038.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Paraffin-NB100-2220-img0038.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Paraffin-NB100-2220-img0038.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Paraffin-NB100-2220-img0038.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Paraffin-NB100-2220-img0048.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Paraffin-NB100-2220-img0048.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Paraffin-NB100-2220-img0048.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-Paraffin-NB100-2220-img0048.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0053.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0053.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0053.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0053.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0062.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0062.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0062.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0062.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Simple-Western-NB100-2220-img0016.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Simple-Western-NB100-2220-img0016.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Simple-Western-NB100-2220-img0016.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/LC3B-Antibody-Simple-Western-NB100-2220-img0016.jpg
applications :
Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, SDS-Page, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Knockdown Validated
USD :
459 USD
alt names :
anti-LC3B, atg8f , Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B ihc, LC3B immunohistochemistry, LC3B immunoprecipitation, LC3B western blot, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B
storage :
Store at -20C.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.