product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rad51 Antibody (14B4) - Azide and BSA Free
catalog :
NB100-148
quantity :
100 ul
price :
529 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
14B4
reactivity :
human, mouse, rat, chicken, roundworm
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay
more info or order :
citations: 68
Reference
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Hinch A, Becker P, Li T, Moralli D, Zhang G, Bycroft C, et al. The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates. Mol Cell. 2020;79:689-701.e10 pubmed publisher
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Feng W, Simpson D, Carvajal Garcia J, Price B, Kumar R, Mose L, et al. Genetic determinants of cellular addiction to DNA polymerase theta. Nat Commun. 2019;10:4286 pubmed publisher
Bai Y, Wang W, Li S, Zhan J, Li H, Zhao M, et al. C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex. Mol Cell. 2019;: pubmed publisher
Tu Z, Mu X, Chen X, Geng Y, Zhang Y, Li Q, et al. Dibutyl phthalate exposure disrupts the progression of meiotic prophase I by interfering with homologous recombination in fetal mouse oocytes. Environ Pollut. 2019;252:388-398 pubmed publisher
Deveryshetty J, Peterlini T, Ryzhikov M, Brahiti N, Dellaire G, Masson J, et al. Novel RNA and DNA strand exchange activity of the PALB2 DNA binding domain and its critical role for DNA repair in cells. elife. 2019;8: pubmed publisher
Shin N, Cuenca L, Karthikraj R, Kannan K, COLAIACOVO M. Assessing effects of germline exposure to environmental toxicants by high-throughput screening in C. elegans. PLoS Genet. 2019;15:e1007975 pubmed publisher
Garcia Muse T, Galindo Diaz U, Garcia Rubio M, Martin J, Polanowska J, O Reilly N, et al. A Meiotic Checkpoint Alters Repair Partner Bias to Permit Inter-sister Repair of Persistent DSBs. Cell Rep. 2019;26:775-787.e5 pubmed publisher
Sulkowski P, Sundaram R, Oeck S, Corso C, Liu Y, Noorbakhsh S, et al. Krebs-cycle-deficient hereditary cancer syndromes are defined by defects in homologous-recombination DNA repair. Nat Genet. 2018;50:1086-1092 pubmed publisher
Periasamy J, Kurdekar V, Jasti S, Nijaguna M, Boggaram S, Hurakadli M, et al. Targeting Phosphopeptide Recognition by the Human BRCA1 Tandem BRCT Domain to Interrupt BRCA1-Dependent Signaling. Cell Chem Biol. 2018;25:677-690.e12 pubmed publisher
Scanlon S, Hegan D, Sulkowski P, Glazer P. Suppression of homology-dependent DNA double-strand break repair induces PARP inhibitor sensitivity in VHL-deficient human renal cell carcinoma. Oncotarget. 2018;9:4647-4660 pubmed publisher
Li Q, Xie W, Wang N, Li C, Wang M. CDC7-dependent transcriptional regulation of RAD54L is essential for tumorigenicity and radio-resistance of glioblastoma. Transl Oncol. 2018;11:300-306 pubmed publisher
Ha K, Ma C, Lin H, Tang L, Lian Z, Zhao F, et al. The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. Nat Commun. 2017;8:15751 pubmed publisher
Nadarajan S, Lambert T, Altendorfer E, Gao J, Blower M, Waters J, et al. Polo-like kinase-dependent phosphorylation of the synaptonemal complex protein SYP-4 regulates double-strand break formation through a negative feedback loop. elife. 2017;6: pubmed publisher
Pauty J, Couturier A, Rodrigue A, Caron M, Coulombe Y, Dellaire G, et al. Cancer-causing mutations in the tumor suppressor PALB2 reveal a novel cancer mechanism using a hidden nuclear export signal in the WD40 repeat motif. Nucleic Acids Res. 2017;45:2644-2657 pubmed publisher
Phillips A, Millet A, Tigano M, Dubois S, Crimmins H, Babin L, et al. Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion. Mol Cell. 2017;65:527-538.e6 pubmed publisher
Li M, Chen Q, Yu X. Chemopreventive Effects of ROS Targeting in a Murine Model of BRCA1-Deficient Breast Cancer. Cancer Res. 2017;77:448-458 pubmed publisher
Ackermann L, Schell M, Pokrzywa W, Kevei Ã, Gartner A, Schumacher B, et al. E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis. Nat Struct Mol Biol. 2016;23:995-1002 pubmed publisher
Dok R, Abbasi Asbagh L, Van Limbergen E, SABLINA A, Nuyts S. Nuclear p16INK4a expression predicts enhanced radiation response in head and neck cancers. Oncotarget. 2016;7:38785-38795 pubmed publisher
Liu H, Yan P, Fanning E. Human DNA helicase B functions in cellular homologous recombination and stimulates Rad51-mediated 5'-3' heteroduplex extension in vitro. PLoS ONE. 2015;10:e0116852 pubmed publisher
Cornago M, Garcia Alberich C, Blasco Angulo N, Vall Llaura N, Nager M, Herreros J, et al. Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe. Cell Death Dis. 2014;5:e1435 pubmed publisher
Del Nagro C, Choi J, Xiao Y, Rangell L, Mohan S, Pandita A, et al. Chk1 inhibition in p53-deficient cell lines drives rapid chromosome fragmentation followed by caspase-independent cell death. Cell Cycle. 2014;13:303-14 pubmed publisher
Wu X, Xu Y, Feng K, Tompkins J, Her C. MutS homologue hMSH5: recombinational DSB repair and non-synonymous polymorphic variants. PLoS ONE. 2013;8:e73284 pubmed publisher
Lans H, Lindvall J, Thijssen K, Karambelas A, Cupac D, Fensg rd O, et al. DNA damage leads to progressive replicative decline but extends the life span of long-lived mutant animals. Cell Death Differ. 2013;20:1709-18 pubmed publisher
Buonanno M, Garty G, Grad M, Gendrel M, Hobert O, Brenner D. Microbeam irradiation of C. elegans nematode in microfluidic channels. Radiat Environ Biophys. 2013;52:531-7 pubmed publisher
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Tompkins J, Wu X, Her C. MutS homologue hMSH5: role in cisplatin-induced DNA damage response. Mol Cancer. 2012;11:10 pubmed publisher
Moldovan G, Dejsuphong D, Petalcorin M, Hofmann K, Takeda S, Boulton S, et al. Inhibition of homologous recombination by the PCNA-interacting protein PARI. Mol Cell. 2012;45:75-86 pubmed publisher
Meerang M, Ritz D, Paliwal S, Garajova Z, Bosshard M, Mailand N, et al. The ubiquitin-selective segregase VCP/p97 orchestrates the response to DNA double-strand breaks. Nat Cell Biol. 2011;13:1376-82 pubmed publisher
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Stewart G, Stankovic T, Byrd P, Wechsler T, Miller E, Huissoon A, et al. RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling. Proc Natl Acad Sci U S A. 2007;104:16910-5 pubmed
Shim K, Schmutte C, Tombline G, Heinen C, Fishel R. hXRCC2 enhances ADP/ATP processing and strand exchange by hRAD51. J Biol Chem. 2004;279:30385-94 pubmed
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Tarsounas M, Davies D, West S. BRCA2-dependent and independent formation of RAD51 nuclear foci. Oncogene. 2003;22:1115-23 pubmed
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product information
master code :
NB100-148
SKU :
NB100-148
product name :
Rad51 Antibody (14B4) - Azide and BSA Free
unit size :
100 ul
description :
The Rad51 Antibody (14B4) - Azide and BSA Free from Novus is a mouse monoclonal antibody to Rad51. This antibody reacts with chicken,human,mouse,rat,nematode - caenorhabditis elegans. The Rad51 Antibody (14B4) - Azide and BSA Free has been validated for the following applications: IF/IHC,Immunocytochemistry/ Immunofluorescence,Chemotaxis,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,In vitro assay,Chromatin Immunoprecipitation (ChIP),Immunohistochemistry,Immunohistochemistry-Frozen,Proximity Ligation Assay,Knockdown Validated.
target :
Rad51
category :
Primary Antibodies
buffer :
PBS, 20% Glycerol (pH7)
clonality :
Monoclonal
clone :
14B4
conjugate :
Unconjugated
host :
Mouse
immunogen :
Full length (amino acids 1-338) Rad51 expressed in E. coli.
isotype :
IgG2b
purity :
Antigen Affinity-purified
species :
Chicken,Human,Mouse,Rat,Nematode - Caenorhabditis elegans
theoretical molecular weight :
37 kDa
gene symbol :
RAD51
Antibody validation :
Biological Validation
accessionNumbers :
Q06609
applications :
IF/IHC,Immunocytochemistry/ Immunofluorescence,Chemotaxis,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,In vitro assay,Chromatin Immunoprecipitation (ChIP),Immunohistochemistry,Immunohistochemistry-Frozen,Proximity Ligation Assay,Knockdown Validated
USD :
529 USD
alt names :
BRCC5, HRAD51, HsRAD51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.