Rad50 Antibody (13B3) - Azide and BSA Free | Novus Biologicals NB100-147-0.1ml product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Rad50 Antibody (13B3) - Azide and BSA Free

catalog :

NB100-147-0.1ml

quantity :

0.1 ml

price :

459 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B3

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Novus Biologicals product webpage

citations: 78

Reference
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Machida K, McNamara G, Cheng K, Huang J, Wang C, Comai L, et al. Hepatitis C virus inhibits DNA damage repair through reactive oxygen and nitrogen species and by interfering with the ATM-NBS1/Mre11/Rad50 DNA repair pathway in monocytes and hepatocytes. J Immunol. 2010;185:6985-98 pubmed publisher
Balasubramanian N, Bai P, Buchek G, Korza G, Weller S. Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex. J Virol. 2010;84:12504-14 pubmed publisher
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Palagyi A, Neveling K, Plinninger U, Ziesch A, Targosz B, Denk G, et al. Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents. Mol Cancer. 2010;9:127 pubmed publisher
Cha H, Lowe J, Li H, Lee J, Belova G, Bulavin D, et al. Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response. Cancer Res. 2010;70:4112-22 pubmed publisher
Lee J, Goodarzi A, Jeggo P, Paull T. 53BP1 promotes ATM activity through direct interactions with the MRN complex. EMBO J. 2010;29:574-85 pubmed publisher
Collaco R, Bevington J, Bhrigu V, Kalman Maltese V, Trempe J. Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways. Virology. 2009;392:24-33 pubmed publisher
Abuzeid W, Jiang X, Shi G, Wang H, Paulson D, Araki K, et al. Molecular disruption of RAD50 sensitizes human tumor cells to cisplatin-based chemotherapy. J Clin Invest. 2009;119:1974-85 pubmed publisher
Waltes R, Kalb R, Gatei M, Kijas A, Stumm M, Sobeck A, et al. Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder. Am J Hum Genet. 2009;84:605-16 pubmed publisher
Bartkova J, Tommiska J, Oplustilova L, Aaltonen K, Tamminen A, Heikkinen T, et al. Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene. Mol Oncol. 2008;2:296-316 pubmed publisher
Schwartz R, Carson C, Schuberth C, Weitzman M. Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase. J Virol. 2009;83:6269-78 pubmed publisher
Zheng L, Kanagaraj R, Mihaljevic B, Schwendener S, Sartori A, Gerrits B, et al. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res. 2009;37:2645-57 pubmed publisher
Karen K, Hoey P, Young C, Hearing P. Temporal regulation of the Mre11-Rad50-Nbs1 complex during adenovirus infection. J Virol. 2009;83:4565-73 pubmed publisher
Carson C, Orazio N, Lee D, Suh J, Bekker Jensen S, Araujo F, et al. Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection. EMBO J. 2009;28:652-62 pubmed publisher
Lakdawala S, Schwartz R, Ferenchak K, Carson C, McSharry B, Wilkinson G, et al. Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation. J Virol. 2008;82:8362-72 pubmed publisher
Cervelli T, Palacios J, Zentilin L, Mano M, Schwartz R, Weitzman M, et al. Processing of recombinant AAV genomes occurs in specific nuclear structures that overlap with foci of DNA-damage-response proteins. J Cell Sci. 2008;121:349-57 pubmed publisher
Gregory D, Bachenheimer S. Characterization of mre11 loss following HSV-1 infection. Virology. 2008;373:124-36 pubmed publisher
Schwartz R, Palacios J, Cassell G, Adam S, Giacca M, Weitzman M. The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. J Virol. 2007;81:12936-45 pubmed
Gorodetsky E, Calkins S, Ahn J, Brooks P. ATM, the Mre11/Rad50/Nbs1 complex, and topoisomerase I are concentrated in the nucleus of Purkinje neurons in the juvenile human brain. DNA Repair (Amst). 2007;6:1698-707 pubmed
Zhong Z, Jiang W, Cesare A, Neumann A, Wadhwa R, Reddel R. Disruption of telomere maintenance by depletion of the MRE11/RAD50/NBS1 complex in cells that use alternative lengthening of telomeres. J Biol Chem. 2007;282:29314-22 pubmed
Bentle M, Reinicke K, Dong Y, Bey E, Boothman D. Nonhomologous end joining is essential for cellular resistance to the novel antitumor agent, beta-lapachone. Cancer Res. 2007;67:6936-45 pubmed
Cho Y, Hong T, Hong S, Guo H, Yu H, Kim D, et al. PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. J Biol Chem. 2007;282:20395-406 pubmed
Bae N, Baumann P. A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends. Mol Cell. 2007;26:323-34 pubmed
Lin X, Liu C, Gao Q, Zhang X, Wu G, Lee W. RINT-1 serves as a tumor suppressor and maintains Golgi dynamics and centrosome integrity for cell survival. Mol Cell Biol. 2007;27:4905-16 pubmed
You K, Li L, Kim N, Kang H, Koh K, Chwae Y, et al. Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors. PLoS Biol. 2007;5:e109 pubmed
Dote H, Burgan W, Camphausen K, Tofilon P. Inhibition of hsp90 compromises the DNA damage response to radiation. Cancer Res. 2006;66:9211-20 pubmed
Takemura H, Rao V, Sordet O, Furuta T, Miao Z, Meng L, et al. Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. J Biol Chem. 2006;281:30814-23 pubmed
Kong L, Meloni A, Nevins J. The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1. Mol Cell. 2006;22:63-71 pubmed
Myers J, Cortez D. Rapid activation of ATR by ionizing radiation requires ATM and Mre11. J Biol Chem. 2006;281:9346-50 pubmed
Tommiska J, Seal S, Renwick A, Barfoot R, Baskcomb L, Jayatilake H, et al. Evaluation of RAD50 in familial breast cancer predisposition. Int J Cancer. 2006;118:2911-6 pubmed
Al Rashid S, Dellaire G, Cuddihy A, Jalali F, Vaid M, Coackley C, et al. Evidence for the direct binding of phosphorylated p53 to sites of DNA breaks in vivo. Cancer Res. 2005;65:10810-21 pubmed
Mongiat Artus P, Miquel C, van der Aa M, Buhard O, Hamelin R, Soliman H, et al. Microsatellite instability and mutation analysis of candidate genes in urothelial cell carcinomas of upper urinary tract. Oncogene. 2006;25:2113-8 pubmed
Liu Y, Shevchenko A, Shevchenko A, Berk A. Adenovirus exploits the cellular aggresome response to accelerate inactivation of the MRN complex. J Virol. 2005;79:14004-16 pubmed
Kim J, Krasieva T, Kurumizaka H, Chen D, Taylor A, Yokomori K. Independent and sequential recruitment of NHEJ and HR factors to DNA damage sites in mammalian cells. J Cell Biol. 2005;170:341-7 pubmed
Koh K, Kang H, Li L, Kim N, You K, Yang E, et al. Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas. Lab Invest. 2005;85:1130-8 pubmed
van Veelen L, Cervelli T, van de Rakt M, Theil A, Essers J, Kanaar R. Analysis of ionizing radiation-induced foci of DNA damage repair proteins. Mutat Res. 2005;574:22-33 pubmed
Lilley C, Carson C, Muotri A, Gage F, Weitzman M. DNA repair proteins affect the lifecycle of herpes simplex virus 1. Proc Natl Acad Sci U S A. 2005;102:5844-9 pubmed
Fasching C, Bower K, Reddel R. Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies. Cancer Res. 2005;65:2722-9 pubmed
Angele S, Jones C, Reis Filho J, Fulford L, Treilleux I, Lakhani S, et al. Expression of ATM, p53, and the MRE11-Rad50-NBS1 complex in myoepithelial cells from benign and malignant proliferations of the breast. J Clin Pathol. 2004;57:1179-84 pubmed
Wu X, Avni D, Chiba T, Yan F, Zhao Q, Lin Y, et al. SV40 T antigen interacts with Nbs1 to disrupt DNA replication control. Genes Dev. 2004;18:1305-16 pubmed
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Pichierri P, Averbeck D, Rosselli F. DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein. Hum Mol Genet. 2002;11:2531-46 pubmed
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Franchitto A, Pichierri P. Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest. J Cell Biol. 2002;157:19-30 pubmed
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product information

master code :

NB100-147

SKU :

NB100-147-0.1ml

product name :

Rad50 Antibody (13B3) - Azide and BSA Free

unit size :

0.1 ml

description :

The Rad50 Antibody (13B3) - Azide and BSA Free from Novus is a mouse monoclonal antibody to Rad50. This antibody reacts with human,monkey,mouse,rat. The Rad50 Antibody (13B3) - Azide and BSA Free has been validated for the following applications: IF/IHC,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry,Immunoprecipitation,In vitro assay,Knockdown Validated.

target :

Rad50

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

13B3

conjugate :

Unconjugated

host :

Mouse

immunogen :

Amino acids 1-425 of Rad50 expressed in E. coli.

isotype :

IgG1

purity :

Protein G purified

species :

Human,Monkey,Mouse,Rat

specificity :

This is specific for Rad50

theoretical molecular weight :

153 kDa

gene symbol :

RAD50

Antibody validation :

Knockout/Knockdown

applications :

IF/IHC,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry,Immunoprecipitation,In vitro assay,Knockdown Validated

USD :

459 USD

alt names :

DNA repair protein RAD50, EC 3.6, EC 3.6.1.15, EC 3.6.3, EC 3.6.3.27, hRAD50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage