Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [HiLyte Fluor 488] | Novus Biologicals NB100-123L product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [HiLyte Fluor 488]

catalog :

NB100-123L

quantity :

0.1 ml

price :

399 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H1alpha67

reactivity :

human, mouse, rat, dog, cow

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:100; fig 1 western blot; mouse; 1:500; fig 5	Schokrpur S, Hu J, Moughon D, Liu P, Lin L, Hermann K, et al. CRISPR-Mediated VHL Knockout Generates an Improved Model for Metastatic Renal Cell Carcinoma. Sci Rep. 2016;6:29032 pubmed publisher
	Murakami A, Wang L, Kalhorn S, Schraml P, Rathmell W, Tan A, et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma. Oncogenesis. 2017;6:e287 pubmed publisher
	Takasaki C, Kobayashi M, Ishibashi H, Akashi T, Okubo K. Expression of hypoxia-inducible factor-1? affects tumor proliferation and antiapoptosis in surgically resected lung cancer. Mol Clin Oncol. 2016;5:295-300 pubmed
	Hoefflin R, Lahrmann B, Warsow G, Hübschmann D, Spath C, Walter B, et al. Spatial niche formation but not malignant progression is a driving force for intratumoural heterogeneity. Nat Commun. 2016;7:ncomms11845 pubmed publisher
	Lee S, Frattini V, Bansal M, Castano A, Sherman D, Hutchinson K, et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016;529:172-7 pubmed publisher
	Narise K, Okuda K, Enomoto Y, Hirayama T, Nagasawa H. Optimization of biguanide derivatives as selective antitumor agents blocking adaptive stress responses in the tumor microenvironment. Drug Des Devel Ther. 2014;8:701-17 pubmed publisher
	Weber D, Tille J, Combescure C, Egger J, Laouiti M, Hammad K, et al. The prognostic value of expression of HIF1?, EGFR and VEGF-A, in localized prostate cancer for intermediate- and high-risk patients treated with radiation therapy with or without androgen deprivation therapy. Radiat Oncol. 2012;7:66 pubmed publisher

product information

Catalog Number :

NB100-123L

Product Name :

Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [HiLyte Fluor 488]

List Price :

399 USD

product type :

Primary Antibodies

Package Size :

0.1 ml

AltNames :

ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1 alpha, HIF1A, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)

Buffer :

PBS

Clonality :

Monoclonal

Clone :

H1alpha67

Conjugate :

HiLyte Fluor 488

Application Summary :

ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Host :

Mouse

Immunogen :

A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665].

Isotype :

IgG2b

Localization :

HIF-1 alpha is a nuclear protein that activates gene transcription in response to reduced cellular O2 concentration.

Preservative :

0.05% Sodium Azide

Purity :

Protein A purified

CrossReactivity :

Avian, Bovine, Canine, Ferret, Human, Mouse, Porcine, Primate, Rat, Sheep

Storage :

Store at 4C in the dark.

Swissprot Human :

Q16665

TMW :

Uses :

This HIF-1 alpha (H1alpha67) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. Chromatin Immunoprecipitation, Gel Super Shift Assays, and ELISA were reported in scientific literature. By Western blot, this antibody recognizes bands at 120kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 120kDa representing post-translational modifications. Nuclear extracts are recommended for Western blot.

Research Area :

Angiogenesis, Cancer, Chromatin Research, Hypoxia, mTOR Pathway, Transcription Factors and Regulators