product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Mouse Monoclonal DNA Polymerase epsilon Antibody
catalog :
NB100-115R
quantity :
0.05 ml
price :
219.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
DyLight 550
reactivity :
human, mouse
application :
western blot
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB100-115R
Product Name :
Mouse Monoclonal DNA Polymerase epsilon Antibody
List Price :
219.00 USD
Product Type :
Primary Antibodies
Package Size :
0.05 ml
AltNames :
CHARAC17arsenic transactivated protein, CHRAC-17, CHRAC17Ybl1, Chromatin accessibility complex 17 kDa protein, DNA polymerase epsilon p17 subunit, DNA polymerase epsilon subunit 3, DNA polymerase epsilon subunit p17, DNA polymerase II subunit 3, EC 2.7.7.7, histone fold protein CHRAC17, huCHRAC17, HuCHRAC17, p17, polymerase (DNA directed), epsilon 3 (p17 subunit), YBL1, Arsenic-transactivated protein, AsTP, asTP
Background :
DNA polymerase epsilon from HeLa cells differs from yeast pol epsilon in that HeLa pol epsilon contains two subunits of 220 and 55 kDa, whereas yeast pol epsilon has one large subunit and three smaller subunits. Previous studies of template specificity of HeLa pol epsilon, which appears to be required for long patch DNA repair in permeabilized cells, indicate pol epsilon prefers long stretches of single-stranded regions in poly(dA)-oligo(dT) as template. HeLa pol alpha and pol delta prefer activated DNA and alternating poly(dA-dT) as templates, respectively. Pol epsilon retains its processivity in the absence of proliferating cell nuclear antigen (PCNA), unlike pol delta. PCNA can also interact with Pol epsilon on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication factor A), and thereby stimulate its primer recognition and DNA synthesis.
Buffer :
50mM sodium borate
Clonality :
Monoclonal
Clone :
3C5.1
Conjugate :
DyLight 550
Application Summary :
Western Blot
Host :
Mouse
Immunogen :
Human DNA Polymerase epsilon purified from HeLa cells [UniProt# Q9NRF9]
Isotype :
IgG2a
Localization :
Nuclear
Preservative :
No Preservative
Purity :
Protein G purified
CrossReactivity :
Human, mouse, monkey and mink samples. This antibody does not work on Drosophilia or the baculovirus host cell pol epsilon, and weakly, if at all, on S. cerivisae pol epsilon.
Specificity :
This is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta.
Storage :
Store at 4C in the dark.
Swissprot Human :
Q9NRF9
TMW :
220 kDa
Uses :
This DNA Polymerase epsilon (3C5.1) antibody can be used in Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB, it detects a single band of 220 kDa (in HeLa extracts) representing the catalytic subunit of DNA Polymerase epsilon. NB100-115 will immunoprecipitate, but only in the absence of salt and even then poorly.
Research Area :
Chromatin Research, DNA Polymerases, DNA Repair
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.