product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Mouse Monoclonal DNA Polymerase epsilon Antibody
catalog :
NB100-115F
quantity :
0.05 ml
price :
209.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
FITC
reactivity :
human, mouse
application :
western blot, immunoprecipitation
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB100-115F
Product Name :
Mouse Monoclonal DNA Polymerase epsilon Antibody
List Price :
209.00 USD
Product Type :
Primary Antibodies
Package Size :
0.05 ml
AltNames :
CHARAC17arsenic transactivated protein, CHRAC-17, CHRAC17Ybl1, Chromatin accessibility complex 17 kDa protein, DNA polymerase epsilon p17 subunit, DNA polymerase epsilon subunit 3, DNA polymerase epsilon subunit p17, DNA polymerase II subunit 3, EC 2.7.7.7, histone fold protein CHRAC17, huCHRAC17, HuCHRAC17, p17, polymerase (DNA directed), epsilon 3 (p17 subunit), YBL1, Arsenic-transactivated protein, AsTP, asTP
Background :
DNA Polymerase epsilon, also called DNA polymerase epsilon subunit 3 (POLE3) is a histone-fold protein which interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. DNA Polymerase epsilon generates a complex with DNA polymerase epsilon subunit CHRAC1 and associates with naked DNA, which is then incorporated into chromatin, facilitated through the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. DNA Polymerase epsilon is a component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4, wherein, the interaction with POLE4 is a prerequisite for further binding with POLE or POLE2. DNA Polymerase epsilon interacts with CHRAC1, and together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRA. DNA Polymerase epsilon histone-fold protein dimers combine within larger enzymatic complexes for facilitating the processes of DNA packaging, replication, and transcription.
Buffer :
50mM sodium borate
Clonality :
Monoclonal
Clone :
3C5.1
Conjugate :
FITC
Application Summary :
Western Blot, Immunoprecipitation
Host :
Mouse
Immunogen :
Human DNA Polymerase epsilon purified from HeLa cells [UniProt# Q9NRF9]
Isotype :
IgG2a
Localization :
Nuclear
Preservative :
No Preservative
Purity :
Protein G purified
CrossReactivity :
Human, mouse, monkey and mink samples. This antibody does not work on Drosophilia or the baculovirus host cell pol epsilon, and weakly, if at all, on S. cerivisae pol epsilon.
Specificity :
This is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta.
Storage :
Store at 4C in the dark.
Swissprot Human :
Q9NRF9
TMW :
220 kDa
Uses :
This DNA Polymerase epsilon (3C5.1) antibody can be used in Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB, it detects a single band of 220 kDa (in HeLa extracts) representing the catalytic subunit of DNA Polymerase epsilon. NB100-115 will immunoprecipitate, but only in the absence of salt and even then poorly.
Research Area :
Chromatin Research, DNA Polymerases, DNA Repair
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.