Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [DyLight 550] | Novus Biologicals NB100-105R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [DyLight 550]

catalog :

NB100-105R

quantity :

0.1 ml

price :

389 USD

clonality :

monoclonal

host :

mouse

conjugate :

DyLight 550

clone name :

H1alpha67

reactivity :

human, mouse, rat, cow

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human	He Q, Gao Z, Yin J, Zhang J, Yun Z, Ye J. Regulation of HIF-1{alpha} activity in adipose tissue by obesity-associated factors: adipogenesis, insulin, and hypoxia. Am J Physiol Endocrinol Metab. 2011;300:E877-85 pubmed publisher

product information

Catalog Number :

NB100-105R

Product Name :

Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [DyLight 550]

List Price :

389 USD

product type :

Primary Antibodies

Package Size :

0.1 ml

AltNames :

ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1 alpha, HIF1A, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)

Buffer :

50mM Sodium Borate

Clonality :

Monoclonal

Clone :

H1alpha67

Conjugate :

DyLight 550

Application Summary :

ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Host :

Mouse

Immunogen :

A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665].

Isotype :

IgG2b

Localization :

HIF-1 is a nuclear protein

Notes :

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Preservative :

0.05% Sodium Azide

Purity :

Protein G purified

CrossReactivity :

Bovine, Ferret, Human, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Xenopus, Yeast

Storage :

Store at 4C in the dark.

Swissprot Human :

Q16665

TMW :

Uses :

This HIF-1 alpha (H1alpha67) antibody is useful for Chromatin Immunoprecipitation (PMID: 21871655), ELISA (PMID: 20042684), Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band can be seen at 120 kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended. This antibody has been used to immunoprecipitate human HIF-1 alpha. For ChIP, refer to research papers with PubMed ID 16204079 and 21871655. This product has been cited for Gel Super Shift Assays in PubMed ID 22411794.

Research Area :

Angiogenesis, Cancer, Chromatin Research, Hypoxia, mTOR Pathway, Transcription Factors and Regulators