Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [HRP] | Novus Biologicals NB100-105H product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [HRP]

catalog :

NB100-105H

quantity :

0.1 ml

price :

389 USD

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

H1alpha67

reactivity :

human, mouse, rat, cow

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 5a	Grillo A, SantaMaria A, Kafina M, Cioffi A, Huston N, Han M, et al. Restored iron transport by a small molecule promotes absorption and hemoglobinization in animals. Science. 2017;356:608-616 pubmed publisher
	Han S, Wang G, Qi X, Lee H, Englander E, Greeley G. A possible role for hypoxia-induced apelin expression in enteric cell proliferation. Am J Physiol Regul Integr Comp Physiol. 2008;294:R1832-9 pubmed publisher

product information

Catalog Number :

NB100-105H

Product Name :

Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [HRP]

List Price :

389 USD

product type :

Primary Antibodies

Package Size :

0.1 ml

AltNames :

ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1 alpha, HIF1A, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)

Buffer :

PBS

Clonality :

Monoclonal

Clone :

H1alpha67

Conjugate :

HRP

Application Summary :

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Host :

Mouse

Immunogen :

A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665].

Isotype :

IgG2b

Localization :

HIF-1 is a nuclear protein

Preservative :

No Preservative

Purity :

Protein G purified

CrossReactivity :

Bovine, Ferret, Human, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Xenopus, Yeast

Storage :

Store at 4C in the dark.

Swissprot Human :

Q16665

TMW :

Uses :

This HIF-1 alpha (H1alpha67) antibody is useful for Chromatin Immunoprecipitation (PMID: 21871655), ELISA (PMID: 20042684), Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band can be seen at 120 kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended. This antibody has been used to immunoprecipitate human HIF-1 alpha. For ChIP, refer to research papers with PubMed ID 16204079 and 21871655. This product has been cited for Gel Super Shift Assays in PubMed ID 22411794.