product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
AIF-1/Iba1 Antibody
catalog :
NB100-1028
quantity :
0.1 mg (also 20 ug)
price :
519 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
biotin
reactivity :
human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 389
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig s2a
  • western blot; mouse; 1:1000; loading ...; fig 2n
Shi H, Yin Z, Koronyo Y, Fuchs D, Sheyn J, Davis M, et al. Regulating microglial miR-155 transcriptional phenotype alleviates Alzheimer's-induced retinal vasculopathy by limiting Clec7a/Galectin-3+ neurodegenerative microglia. Acta Neuropathol Commun. 2022;10:136 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 5a
Zhao Q, Dai W, Chen H, Jacobs R, Zlokovic B, Lund B, et al. Prenatal disruption of blood-brain barrier formation via cyclooxygenase activation leads to lifelong brain inflammation. Proc Natl Acad Sci U S A. 2022;119:e2113310119 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 3a
Yu S, Cheng L, Tian D, Li Z, Yao F, Luo Y, et al. Fascin-1 is Highly Expressed Specifically in Microglia After Spinal Cord Injury and Regulates Microglial Migration. Front Pharmacol. 2021;12:729524 pubmed publisher
  • immunohistochemistry; human; 1:500; loading ...; fig 1a
Huang Y, Happonen K, Burrola P, O Connor C, Hah N, Huang L, et al. Microglia use TAM receptors to detect and engulf amyloid β plaques. Nat Immunol. 2021;22:586-594 pubmed publisher
  • immunohistochemistry; rat; 1:200; loading ...; fig 3b
Xu H, Wang Y, Luo Y. OTULIN is a new target of EA treatment in the alleviation of brain injury and glial cell activation via suppression of the NF-κB signalling pathway in acute ischaemic stroke rats. Mol Med. 2021;27:37 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 5a
Ping S, Qiu X, Kyle M, Zhao L. Brain-derived CCR5 Contributes to Neuroprotection and Brain Repair after Experimental Stroke. Aging Dis. 2021;12:72-92 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 6a
Yoon S, Bae Y, Oh S, Song W, Chang H, Kim M. Altered hippocampal gene expression, glial cell population, and neuronal excitability in aminopeptidase P1 deficiency. Sci Rep. 2021;11:932 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig 1a
Li Z, Song Y, He T, Wen R, Li Y, Chen T, et al. M2 microglial small extracellular vesicles reduce glial scar formation via the miR-124/STAT3 pathway after ischemic stroke in mice. Theranostics. 2021;11:1232-1248 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig s1f
Zhang X, Wang R, Hu D, Sun X, Fujioka H, Lundberg K, et al. Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease. Sci Adv. 2020;6: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:250; loading ...; fig 6h
Cignarella F, Filipello F, Bollman B, Cantoni C, Locca A, Mikesell R, et al. TREM2 activation on microglia promotes myelin debris clearance and remyelination in a model of multiple sclerosis. Acta Neuropathol. 2020;140:513-534 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; fig 3d
Dulken B, Buckley M, Navarro Negredo P, Saligrama N, Cayrol R, Leeman D, et al. Single-cell analysis reveals T cell infiltration in old neurogenic niches. Nature. 2019;571:205-210 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 7i
Diéguez Hurtado R, Kato K, Giaimo B, Nieminen Kelhä M, Arf H, Ferrante F, et al. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes. Nat Commun. 2019;10:2817 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5a
Wang E, Dai Z, Ferrante A, Drake C, Christiano A. A Subset of TREM2+ Dermal Macrophages Secretes Oncostatin M to Maintain Hair Follicle Stem Cell Quiescence and Inhibit Hair Growth. Cell Stem Cell. 2019;: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 3c
Hou Y, Lautrup S, Cordonnier S, Wang Y, Croteau D, Zavala E, et al. NAD+ supplementation normalizes key Alzheimer's features and DNA damage responses in a new AD mouse model with introduced DNA repair deficiency. Proc Natl Acad Sci U S A. 2018;115:E1876-E1885 pubmed publisher
  • immunohistochemistry; rat; 1:50; loading ...; fig s1e
Zhang R, Wu Y, Xie F, Zhong Y, Wang Y, Xu M, et al. RGMa mediates reactive astrogliosis and glial scar formation through TGF?1/Smad2/3 signaling after stroke. Cell Death Differ. 2018;25:1503-1516 pubmed publisher
  • western blot; mouse; 1:300
Choi M, Ahn S, Yang E, Kim H, Chong Y, Kim H. Hippocampus-based contextual memory alters the morphological characteristics of astrocytes in the dentate gyrus. Mol Brain. 2016;9:72 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 6a
Loewen J, Barker Haliski M, Dahle E, White H, Wilcox K. Neuronal Injury, Gliosis, and Glial Proliferation in Two Models of Temporal Lobe Epilepsy. J Neuropathol Exp Neurol. 2016;75:366-78 pubmed publisher
Franco A, Martini H, Victorelli S, Lagnado A, Wyles S, Rowsey J, et al. Senescent cell transplantation into the skin induces age-related peripheral dysfunction and cognitive decline. Aging Cell. 2025;24:e14340 pubmed publisher
Horiuchi M, Watanabe S, Komine O, Takahashi E, Kaneko K, Itohara S, et al. ALS-linked mutant TDP-43 in oligodendrocytes induces oligodendrocyte damage and exacerbates motor dysfunction in mice. Acta Neuropathol Commun. 2024;12:184 pubmed publisher
Chen J, Crouch E, Zawadzki M, Jacobs K, Mayo L, Choi J, et al. Proinflammatory immune cells disrupt angiogenesis and promote germinal matrix hemorrhage in prenatal human brain. Nat Neurosci. 2024;27:2115-2129 pubmed publisher
Makabe K, Okada H, Tachibana N, Ishikura H, Ito N, Tanaka M, et al. Baricitinib ameliorates inflammatory and neuropathic pain in collagen antibody-induced arthritis mice by modulating the IL-6/JAK/STAT3 pathway and CSF-1 expression in dorsal root ganglion neurons. Arthritis Res Ther. 2024;26:121 pubmed publisher
Kang S, Ko E, Andrews A, Shin J, Nance K, Barman P, et al. Microglia undergo sex-dimorphic transcriptional and metabolic rewiring during aging. J Neuroinflammation. 2024;21:150 pubmed publisher
Ahmed C, Greve H, Garza Lombo C, Malley J, Johnson J, Oblak A, et al. Peripheral HMGB1 is linked to O3 pathology of disease-associated astrocytes and amyloid. Alzheimers Dement. 2024;20:3551-3566 pubmed publisher
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Schrader J, Xu F, Agostinucci K, DaSilva N, Van Nostrand W. Longitudinal markers of cerebral amyloid angiopathy and related inflammation in rTg-DI rats. Sci Rep. 2024;14:8441 pubmed publisher
ABCOUWER S, Miglioranza Scavuzzi B, Kish P, Kong D, Shanmugam S, Le X, et al. The mouse retinal pigment epithelium mounts an innate immune defense response following retinal detachment. J Neuroinflammation. 2024;21:74 pubmed publisher
Wang T, Sobue A, Watanabe S, Komine O, Saido T, Saito T, et al. Dimethyl fumarate improves cognitive impairment and neuroinflammation in mice with Alzheimer's disease. J Neuroinflammation. 2024;21:55 pubmed publisher
Hagen K, Gordon P, Frederick A, Palmer A, Edalat P, Zonta Y, et al. CRYAB plays a role in terminating the presence of pro-inflammatory macrophages in the older, injured mouse peripheral nervous system. Neurobiol Aging. 2024;133:1-15 pubmed publisher
Huang Z, Gong Z, Lin Y, Yang F, Chen W, Xiang S, et al. Treatment with glatiramer acetate in APPswe/PS1dE9 mice at an early stage of Alzheimer's disease prior to amyloid-beta deposition delays the disease's pathological development and ameliorates cognitive decline. Front Aging Neurosci. 2024;16:1267780 pubmed publisher
Jiang W, Zhou Y, Wu W, Yang L, Wu Y, Zhang X, et al. A circRNA ceRNA network involved in cognitive dysfunction after chronic cerebral hypoperfusion. Aging (Albany NY). 2024;16:1161-1181 pubmed publisher
Silveira Prudente A, Hoon Lee S, Roh J, Lückemeyer D, Cohen C, Pertin M, et al. Microglial STING activation alleviates nerve injury-induced neuropathic pain in male but not female mice. Brain Behav Immun. 2024;117:51-65 pubmed publisher
Cui Y, Zhang Z, Lv M, Duan Z, Liu W, Gao J, et al. Chromatin target of protein arginine methyltransferases alleviates cerebral ischemia/reperfusion-induced injury by regulating RNA alternative splicing. iScience. 2024;27:108688 pubmed publisher
Denaro S, Pasquinucci L, Turnaturi R, Alberghina C, Longhitano L, Giallongo S, et al. Sigma-1 Receptor Inhibition Reduces Mechanical Allodynia and Modulate Neuroinflammation in Chronic Neuropathic Pain. Mol Neurobiol. 2024;61:2672-2685 pubmed publisher
Wang S, Jiang C, Cao K, Li R, Gao Z, Wang Y. HK2 in microglia and macrophages contribute to the development of neuropathic pain. Glia. 2024;72:396-410 pubmed publisher
Thougaard E, Carney B, Wlodarczyk A, Brambilla R, Lambertsen K. Peripherally derived myeloid cells induce disease-dependent phenotypic changes in microglia. Front Cell Neurosci. 2023;17:1295840 pubmed publisher
Morton L, Arndt P, Garza A, Henneicke S, Mattern H, Gonzalez M, et al. Spatio-temporal dynamics of microglia phenotype in human and murine cSVD: impact of acute and chronic hypertensive states. Acta Neuropathol Commun. 2023;11:204 pubmed publisher
Lindquist K, Shein S, Hovhannisyan A, Mecklenburg J, Zou Y, Lai Z, et al. Associations of tissue damage induced inflammatory plasticity in masseter muscle with the resolution of chronic myalgia. Sci Rep. 2023;13:22057 pubmed publisher
Chen Y, Song S, Parhizkar S, Lord J, Zhu Y, Strickland M, et al. APOE3ch alters microglial response and suppresses Aβ-induced tau seeding and spread. Cell. 2023;: pubmed publisher
Kaya Z, Belder N, Sever Bahcekapili M, Dönmez Demir B, Erdener x, Bozbeyoglu N, et al. Vesicular HMGB1 release from neurons stressed with spreading depolarization enables confined inflammatory signaling to astrocytes. J Neuroinflammation. 2023;20:295 pubmed publisher
Feng T, DU H, Hu F. Loss of TMEM106B exacerbates Tau pathology and neurodegeneration in PS19 mice. bioRxiv. 2023;: pubmed publisher
Wang Y, Dang Z, Wang X, Chen Y, Dong P, Liu G, et al. Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice. Biochem Biophys Rep. 2023;36:101565 pubmed publisher
Zamith Cunha R, Semprini A, Salamanca G, Gobbo F, Morini M, Pickles K, et al. Expression of Cannabinoid Receptors in the Trigeminal Ganglion of the Horse. Int J Mol Sci. 2023;24: pubmed publisher
Connolly K, Margaria J, Di Biase E, Cooper O, Hallett P, Isacson O. Loss of Lipid Carrier ApoE Exacerbates Brain Glial and Inflammatory Responses after Lysosomal GBA1 Inhibition. Cells. 2023;12: pubmed publisher
Wu D, Liu J, Liu J, Ge W, Wu S, Zeng C, et al. Immune pathway activation in neurons triggers neural damage after stroke. Cell Rep. 2023;42:113368 pubmed publisher
Groh J, Abdelwahab T, Kattimani Y, H xf6 rner M, Loserth S, Gudi V, et al. Microglia-mediated demyelination protects against CD8+ T cell-driven axon degeneration in mice carrying PLP defects. Nat Commun. 2023;14:6911 pubmed publisher
Wang J, Xiao X, He X, He B, Liu C, Teng Z. Agomir-331 Suppresses Reactive Gliosis and Neuroinflammation after Traumatic Brain Injury. Cells. 2023;12: pubmed publisher
Cao H, Li B, Mu M, Li S, Chen H, Tao H, et al. Nicotine suppresses crystalline silica-induced astrocyte activation and neuronal death by inhibiting NF-κB in the mouse hippocampus. CNS Neurosci Ther. 2023;: pubmed publisher
Zeballos C M, Moore H, Smith T, Powell J, Ahsan N, Zhang S, et al. Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteins. Nat Commun. 2023;14:6492 pubmed publisher
Colvett I, Gilmore A, Guzman S, Ledreux A, Quintero J, Ginjupally D, et al. Recipient Reaction and Composition of Autologous Sural Nerve Tissue Grafts into the Human Brain. J Clin Med. 2023;12: pubmed publisher
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Zamith Cunha R, Salamanca G, Mille F, Delprete C, Franciosi C, Piva G, et al. Endocannabinoid System Receptors at the Hip and Stifle Joints of Middle-Aged Dogs: A Novel Target for the Therapeutic Use of Cannabis sativa Extract in Canine Arthropathies. Animals (Basel). 2023;13: pubmed publisher
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Holden J, Wareham L, Calkins D. Retinal astrocyte morphology predicts integration of vascular and neuronal architecture. Front Neurosci. 2023;17:1244679 pubmed publisher
Yang X, Cao Q, Guo Y, He J, Xu D, Lin A. GSDMD knockdown attenuates phagocytic activity of microglia and exacerbates seizure susceptibility in TLE mice. J Neuroinflammation. 2023;20:193 pubmed publisher
Crivelli S, Quadri Z, Vekaria H, Zhu Z, Tripathi P, Elsherbini A, et al. Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouse. Acta Neuropathol Commun. 2023;11:135 pubmed publisher
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Cao M, Huang X, Zou J, Peng Y, Wang Y, Zheng X, et al. Attenuation of Microglial Activation and Pyroptosis by Inhibition of P2X7 Pathway Promotes Photoreceptor Survival in Experimental Retinal Detachment. Invest Ophthalmol Vis Sci. 2023;64:34 pubmed publisher
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Ali T, Klein A, Vu A, Arifin M, Hannaoui S, Gilch S. Peptide aptamer targeting Aβ-PrP-Fyn axis reduces Alzheimer's disease pathologies in 5XFAD transgenic mouse model. Cell Mol Life Sci. 2023;80:139 pubmed publisher
Zhang T, Pang W, Feng T, Guo J, Wu K, Nunez Santos M, et al. TMEM106B regulates microglial proliferation and survival in response to demyelination. Sci Adv. 2023;9:eadd2676 pubmed publisher
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Chen X, Chen L, Fan L, Kan H, Wang Z, Qian B, et al. Microglial P2Y12 Signaling Contributes to Cisplatin-induced Pain Hypersensitivity via IL-18-mediated Central Sensitization in the Spinal Cord. J Pain. 2023;24:901-917 pubmed publisher
van Lengerich B, Zhan L, Xia D, Chan D, Joy D, Park J, et al. A TREM2-activating antibody with a blood-brain barrier transport vehicle enhances microglial metabolism in Alzheimer's disease models. Nat Neurosci. 2023;26:416-429 pubmed publisher
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Gupta D, Lee Y, Choe Y, Kim K, Song G, Hwang S. Knee osteoarthritis accelerates amyloid beta deposition and neurodegeneration in a mouse model of Alzheimer's disease. Mol Brain. 2023;16:1 pubmed publisher
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product information
master code :
NB100-1028
SKU :
NB100-1028
product name :
AIF-1/Iba1 Antibody
unit size :
0.1 mg (also 20 ug)
description :
The AIF-1/Iba1 Antibody from Novus is a goat polyclonal antibody to AIF-1/Iba1. This antibody reacts with canine,equine,feline,human,mouse,porcine,rat. The AIF-1/Iba1 Antibody has been validated for the following applications: Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,IF/IHC,IHC-F,Immunoprecipitation,Immunohistochemistry Free-Floating,Immunohistochemistry Whole-Mount,Immunocytochemistry,Knockout Validated,Dual RNAscope ISH-IHC,Peptide ELISA.
target :
AIF-1/Iba1
category :
Primary Antibodies
buffer :
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
clonality :
Polyclonal
concentration :
0.5 mg/ml
conjugate :
Unconjugated
host :
Goat
immunogen :
This AIF-1/Iba1 Antibody was developed against a peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
isotype :
IgG
purity :
Immunogen affinity purified
species :
Canine,Equine,Feline,Human,Mouse,Porcine,Rat
specificity :
This AIF-1/Iba1 Antibody is expected to recognize isoform 1 (NP_116573
theoretical molecular weight :
16.8 kDa
gene symbol :
AIF1
accessionNumbers :
NP_116573.1, NP_001614.3
applications :
Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,IF/IHC,IHC-F,Immunoprecipitation,Immunohistochemistry Free-Floating,Immunohistochemistry Whole-Mount,Immunocytochemistry,Knockout Validated,Dual RNAscope ISH-IHC,Peptide ELISA
USD :
519 USD
alt names :
AIF-1, allograft inflammatory factor 1, G1, IBA1, IbaI, interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, IRT1, IRT-1, Protein G1
storage :
Store at -20C. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.