product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
APE Antibody - BSA Free
catalog :
NB100-101
quantity :
0.1 ml (also 0.025 ml)
price :
409 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
6H12
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Novus Biologicals product webpage
citations: 24
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:2000; fig 3
Nassour H, Wang Z, Saad A, Papaluca A, Brosseau N, Affar E, et al. Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression. Sci Rep. 2016;6:29389 pubmed publisher
  • immunohistochemistry - paraffin section; human; 0.1 ug/ml; fig st1
  • western blot; human; fig 3
Kaur A, Webster M, Marchbank K, Behera R, Ndoye A, Kugel C, et al. sFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance. Nature. 2016;532:250-4 pubmed publisher
Hartman G, Muniyandi A, Sishtla K, Kpenu E, Miller W, Kaplan B, et al. Ref-1 redox activity regulates retinal neovascularization by modulating transcriptional activation of HIF-1α. FASEB J. 2025;39:e70348 pubmed publisher
Mijit M, Kpenu E, Chowdhury N, Gampala S, Wireman R, Liu S, et al. In vitro and In vivo evidence demonstrating chronic absence of Ref-1 Cysteine 65 impacts Ref-1 folding configuration, redox signaling, proliferation and metastasis in pancreatic cancer. Redox Biol. 2024;69:102977 pubmed publisher
Caston R, Fortini P, Chen K, Bauer J, Dogliotti E, Yin Y, et al. Maintenance of Flap Endonucleases for Long-Patch Base Excision DNA Repair in Mouse Muscle and Neuronal Cells Differentiated In Vitro. Int J Mol Sci. 2023;24: pubmed publisher
Ito M, Ducasa G, Molina J, Santos J, Mallela S, Kim J, et al. ABCA1 deficiency contributes to podocyte pyroptosis priming via the APE1/IRF1 axis in diabetic kidney disease. Sci Rep. 2023;13:9616 pubmed publisher
Algethami M, Toss M, Woodcock C, Jaipal C, Brownlie J, Shoqafi A, et al. Unravelling the clinicopathological and functional significance of replication protein A (RPA) heterotrimeric complex in breast cancers. NPJ Breast Cancer. 2023;9:18 pubmed publisher
Xue Z, Demple B. Knockout and Inhibition of Ape1: Roles of Ape1 in Base Excision DNA Repair and Modulation of Gene Expression. Antioxidants (Basel). 2022;11: pubmed publisher
Miner K, Jamenis A, Bhatia T, Clark R, Rajasundaram D, Sauvaigo S, et al. α-synucleinopathy exerts sex-dimorphic effects on the multipurpose DNA repair/redox protein APE1 in mice and humans. Prog Neurobiol. 2022;216:102307 pubmed publisher
Alblihy A, Shoqafi A, Toss M, Algethami M, Harris A, Jeyapalan J, et al. Untangling the clinicopathological significance of MRE11-RAD50-NBS1 complex in sporadic breast cancers. NPJ Breast Cancer. 2021;7:143 pubmed publisher
Kim D, Kulishova L, Torgasheva N, Melentyev V, Dianov G, Medvedev S, et al. Mild phenotype of knockouts of the major apurinic/apyrimidinic endonuclease APEX1 in a non-cancer human cell line. PLoS ONE. 2021;16:e0257473 pubmed publisher
Quinones J, Thapar U, Wilson S, Ramsden D, Demple B. Oxidative DNA-protein crosslinks formed in mammalian cells by abasic site lyases involved in DNA repair. DNA Repair (Amst). 2020;87:102773 pubmed publisher
Zhang Q, Chao T, Patil V, Qin Y, Tiwari S, Chiou J, et al. The long noncoding RNA ROCKI regulates inflammatory gene expression. EMBO J. 2019;38: pubmed publisher
Pascut D, Sukowati C, Antoniali G, Mangiapane G, Burra S, Mascaretti L, et al. Serum AP-endonuclease 1 (sAPE1) as novel biomarker for hepatocellular carcinoma. Oncotarget. 2019;10:383-394 pubmed publisher
Kumar S, Talluri S, Pal J, Yuan X, Lu R, Nanjappa P, et al. Role of apurinic/apyrimidinic nucleases in the regulation of homologous recombination in myeloma: mechanisms and translational significance. Blood Cancer J. 2018;8:92 pubmed publisher
Logsdon D, Shah F, Carta F, Supuran C, Kamocka M, Jacobsen M, et al. Blocking HIF signaling via novel inhibitors of CA9 and APE1/Ref-1 dramatically affects pancreatic cancer cell survival. Sci Rep. 2018;8:13759 pubmed publisher
Fehrenbacher J, Guo C, Kelley M, Vasko M. DNA damage mediates changes in neuronal sensitivity induced by the inflammatory mediators, MCP-1 and LPS, and can be reversed by enhancing the DNA repair function of APE1. Neuroscience. 2017;366:23-35 pubmed publisher
Shah F, Logsdon D, Messmann R, Fehrenbacher J, Fishel M, Kelley M. Exploiting the Ref-1-APE1 node in cancer signaling and other diseases: from bench to clinic. NPJ Precis Oncol. 2017;1: pubmed publisher
Sandomenico A, Focà A, Sanguigno L, Caporale A, Focà G, Pignalosa A, et al. Monoclonal antibodies against pools of mono- and polyacetylated peptides selectively recognize acetylated lysines within the context of the original antigen. MAbs. 2016;8:1575-1589 pubmed
Logsdon D, Grimard M, Luo M, Shahda S, Jiang Y, Tong Y, et al. Regulation of HIF1α under Hypoxia by APE1/Ref-1 Impacts CA9 Expression: Dual Targeting in Patient-Derived 3D Pancreatic Cancer Models. Mol Cancer Ther. 2016;15:2722-2732 pubmed
Poletto M, Legrand A, Fletcher S, Dianov G. p53 coordinates base excision repair to prevent genomic instability. Nucleic Acids Res. 2016;44:3165-75 pubmed publisher
Sanchez J, Reddick T, Perez M, Centonze V, Mitra S, Izumi T, et al. Increased human AP endonuclease 1 level confers protection against the paternal age effect in mice. Mutat Res. 2015;779:124-33 pubmed publisher
Quiñones J, Thapar U, Yu K, Fang Q, Sobol R, Demple B. Enzyme mechanism-based, oxidative DNA-protein cross-links formed with DNA polymerase β in vivo. Proc Natl Acad Sci U S A. 2015;112:8602-7 pubmed publisher
Iglesias Ara A, Zenarruzabeitia O, Buelta L, Merino J, Zubiaga A. E2F1 and E2F2 prevent replicative stress and subsequent p53-dependent organ involution. Cell Death Differ. 2015;22:1577-89 pubmed publisher
product information
master code :
NB100-101
SKU :
NB100-101
product name :
APE Antibody - BSA Free
unit size :
0.1 ml (also 0.025 ml)
description :
The APE Antibody - BSA Free from Novus is a rabbit polyclonal antibody to APE. This antibody reacts with human,mouse,primate,rabbit,rat. The APE Antibody - BSA Free has been validated for the following applications: Immunohistochemistry,Western Blot,Block/Neutralize,Chemotaxis,IF/IHC,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Flow Cytometry,Immunohistochemistry-Frozen,Flow (Intracellular),Immunohistochemistry Free-Floating,Simple Western,Chromatin Immunoprecipitation (ChIP).
target :
APE
category :
Primary Antibodies
buffer :
PBS
clonality :
Polyclonal
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Rabbit
immunogen :
Affinity purified human APE1 [UniProt# P27695]
isotype :
IgG
purity :
Immunogen affinity purified
species :
Human,Mouse,Primate,Rabbit,Rat
theoretical molecular weight :
37 kDa
gene symbol :
APEX1
applications :
Western Blot,Block/Neutralize,Immunohistochemistry,Chemotaxis,IF/IHC,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Flow Cytometry,Immunohistochemistry-Frozen,Flow (Intracellular),Immunohistochemistry Free-Floating,Simple Western,Chromatin Immunoprecipitation (ChIP)
USD :
409 USD
alt names :
AP endonuclease 1, APE, APE1, APE-1, APEAPEX nuclease (multifunctional DNA repair enzyme), APENAP endonuclease class I, APEX deoxyribonuclease (apurinic or apyrimidinic), APEX nuclease, APEX nuclease (multifunctional DNA repair enzyme) 1, Apurinic-apyrimidinic endonuclease 1, APXAP lyase, DNA-(apurinic or apyrimidinic site) lyase, EC 3.1, EC 4.2.99.18, HAP1apurinic/apyrimidinic (abasic) endonuclease, multifunctional DNA repair enzyme, protein REF-1, redox factor 1, Redox factor-1, REF-1, REF1 apurinic/apyrimidinic exonuclease
storage :
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.