product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
APE Antibody - BSA Free
catalog :
NB100-101
quantity :
0.1 ml (also 0.025 ml)
price :
409 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
6H12
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Novus Biologicals product webpage
citations: 25
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:2000; fig 3
Nassour H, Wang Z, Saad A, Papaluca A, Brosseau N, Affar E, et al. Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression. Sci Rep. 2016;6:29389 pubmed publisher
  • immunohistochemistry - paraffin section; human; 0.1 ug/ml; fig st1
  • western blot; human; fig 3
Kaur A, Webster M, Marchbank K, Behera R, Ndoye A, Kugel C, et al. sFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance. Nature. 2016;532:250-4 pubmed publisher
Hartman G, Muniyandi A, Sishtla K, Kpenu E, Miller W, Kaplan B, et al. Ref-1 redox activity regulates retinal neovascularization by modulating transcriptional activation of HIF-1α. FASEB J. 2025;39:e70348 pubmed publisher
Gotoh N, Oda T, Kitamura Y, Shiraishi N, Aoyagi R, Omori A, et al. APEX1 Polymorphisms Affect Acute Myeloid Leukemia Risk, and Its Expression Is Involved in Cell Proliferation and Differentiation. Int J Lab Hematol. 2025;47:276-287 pubmed publisher
Mijit M, Kpenu E, Chowdhury N, Gampala S, Wireman R, Liu S, et al. In vitro and In vivo evidence demonstrating chronic absence of Ref-1 Cysteine 65 impacts Ref-1 folding configuration, redox signaling, proliferation and metastasis in pancreatic cancer. Redox Biol. 2024;69:102977 pubmed publisher
Caston R, Fortini P, Chen K, Bauer J, Dogliotti E, Yin Y, et al. Maintenance of Flap Endonucleases for Long-Patch Base Excision DNA Repair in Mouse Muscle and Neuronal Cells Differentiated In Vitro. Int J Mol Sci. 2023;24: pubmed publisher
Ito M, Ducasa G, Molina J, Santos J, Mallela S, Kim J, et al. ABCA1 deficiency contributes to podocyte pyroptosis priming via the APE1/IRF1 axis in diabetic kidney disease. Sci Rep. 2023;13:9616 pubmed publisher
Algethami M, Toss M, Woodcock C, Jaipal C, Brownlie J, Shoqafi A, et al. Unravelling the clinicopathological and functional significance of replication protein A (RPA) heterotrimeric complex in breast cancers. NPJ Breast Cancer. 2023;9:18 pubmed publisher
Xue Z, Demple B. Knockout and Inhibition of Ape1: Roles of Ape1 in Base Excision DNA Repair and Modulation of Gene Expression. Antioxidants (Basel). 2022;11: pubmed publisher
Miner K, Jamenis A, Bhatia T, Clark R, Rajasundaram D, Sauvaigo S, et al. α-synucleinopathy exerts sex-dimorphic effects on the multipurpose DNA repair/redox protein APE1 in mice and humans. Prog Neurobiol. 2022;216:102307 pubmed publisher
Alblihy A, Shoqafi A, Toss M, Algethami M, Harris A, Jeyapalan J, et al. Untangling the clinicopathological significance of MRE11-RAD50-NBS1 complex in sporadic breast cancers. NPJ Breast Cancer. 2021;7:143 pubmed publisher
Kim D, Kulishova L, Torgasheva N, Melentyev V, Dianov G, Medvedev S, et al. Mild phenotype of knockouts of the major apurinic/apyrimidinic endonuclease APEX1 in a non-cancer human cell line. PLoS ONE. 2021;16:e0257473 pubmed publisher
Quinones J, Thapar U, Wilson S, Ramsden D, Demple B. Oxidative DNA-protein crosslinks formed in mammalian cells by abasic site lyases involved in DNA repair. DNA Repair (Amst). 2020;87:102773 pubmed publisher
Zhang Q, Chao T, Patil V, Qin Y, Tiwari S, Chiou J, et al. The long noncoding RNA ROCKI regulates inflammatory gene expression. EMBO J. 2019;38: pubmed publisher
Pascut D, Sukowati C, Antoniali G, Mangiapane G, Burra S, Mascaretti L, et al. Serum AP-endonuclease 1 (sAPE1) as novel biomarker for hepatocellular carcinoma. Oncotarget. 2019;10:383-394 pubmed publisher
Kumar S, Talluri S, Pal J, Yuan X, Lu R, Nanjappa P, et al. Role of apurinic/apyrimidinic nucleases in the regulation of homologous recombination in myeloma: mechanisms and translational significance. Blood Cancer J. 2018;8:92 pubmed publisher
Logsdon D, Shah F, Carta F, Supuran C, Kamocka M, Jacobsen M, et al. Blocking HIF signaling via novel inhibitors of CA9 and APE1/Ref-1 dramatically affects pancreatic cancer cell survival. Sci Rep. 2018;8:13759 pubmed publisher
Fehrenbacher J, Guo C, Kelley M, Vasko M. DNA damage mediates changes in neuronal sensitivity induced by the inflammatory mediators, MCP-1 and LPS, and can be reversed by enhancing the DNA repair function of APE1. Neuroscience. 2017;366:23-35 pubmed publisher
Shah F, Logsdon D, Messmann R, Fehrenbacher J, Fishel M, Kelley M. Exploiting the Ref-1-APE1 node in cancer signaling and other diseases: from bench to clinic. NPJ Precis Oncol. 2017;1: pubmed publisher
Sandomenico A, Focà A, Sanguigno L, Caporale A, Focà G, Pignalosa A, et al. Monoclonal antibodies against pools of mono- and polyacetylated peptides selectively recognize acetylated lysines within the context of the original antigen. MAbs. 2016;8:1575-1589 pubmed
Logsdon D, Grimard M, Luo M, Shahda S, Jiang Y, Tong Y, et al. Regulation of HIF1α under Hypoxia by APE1/Ref-1 Impacts CA9 Expression: Dual Targeting in Patient-Derived 3D Pancreatic Cancer Models. Mol Cancer Ther. 2016;15:2722-2732 pubmed
Poletto M, Legrand A, Fletcher S, Dianov G. p53 coordinates base excision repair to prevent genomic instability. Nucleic Acids Res. 2016;44:3165-75 pubmed publisher
Sanchez J, Reddick T, Perez M, Centonze V, Mitra S, Izumi T, et al. Increased human AP endonuclease 1 level confers protection against the paternal age effect in mice. Mutat Res. 2015;779:124-33 pubmed publisher
Quiñones J, Thapar U, Yu K, Fang Q, Sobol R, Demple B. Enzyme mechanism-based, oxidative DNA-protein cross-links formed with DNA polymerase β in vivo. Proc Natl Acad Sci U S A. 2015;112:8602-7 pubmed publisher
Iglesias Ara A, Zenarruzabeitia O, Buelta L, Merino J, Zubiaga A. E2F1 and E2F2 prevent replicative stress and subsequent p53-dependent organ involution. Cell Death Differ. 2015;22:1577-89 pubmed publisher
product information
brand :
Novus
catalog number base :
NB100-101
SKU :
NB100-101
product name :
APE Antibody - BSA Free
units size :
0.1 ml (also 0.025 ml)
description :
The APE Antibody - BSA Free from Novus is a rabbit polyclonal antibody to APE. This antibody reacts with human,mouse,primate,rabbit,rat. The APE Antibody - BSA Free has been validated for the following applications: Chemotaxis,IF/IHC,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Flow (Intracellular),Immunohistochemistry Free-Floating,Simple Western,Chromatin Immunoprecipitation (ChIP),Block/Neutralize,Immunocytochemistry/ Immunofluorescence.
target :
APE
category :
Primary Antibodies
buffer :
PBS
clonality :
Polyclonal
concentration :
1.0 mg/ml
conjugate :
Unconjugated
all_available conjugate :
Unconjugated
host :
Rabbit
immunogen :
Affinity purified human APE1 [UniProt# P27695]
isotype :
IgG
purity :
Immunogen affinity purified
species :
Human,Mouse,Primate,Rabbit,Rat
theoretical molecular weight :
37 kDa
gene symbol :
APEX1
review stars :
5
top caption :
Simple Western: APE AntibodyBSA Free [NB100-101]
applications :
Chemotaxis,IF/IHC,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Flow (Intracellular),Immunohistochemistry Free-Floating,Simple Western,Chromatin Immunoprecipitation (ChIP),Block/Neutralize,Immunocytochemistry/ Immunofluorescence
USD :
409 USD
alt names :
AP endonuclease 1, APE, APE1, APE-1, APEAPEX nuclease (multifunctional DNA repair enzyme), APENAP endonuclease class I, APEX deoxyribonuclease (apurinic or apyrimidinic), APEX nuclease, APEX nuclease (multifunctional DNA repair enzyme) 1, Apurinic-apyrimidinic endonuclease 1, APXAP lyase, DNA-(apurinic or apyrimidinic site) lyase, EC 3.1, EC 4.2.99.18, HAP1apurinic/apyrimidinic (abasic) endonuclease, multifunctional DNA repair enzyme, protein REF-1, redox factor 1, Redox factor-1, REF-1, REF1 apurinic/apyrimidinic exonuclease
storage :
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.