domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
reactivity: human
application: western blot, immunoprecipitation
Western Blot: Myosin Phosphatase Antibody [NB110-38889] - Detection of human MYPT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MYPT1 antibody NB110-38889 (lot NB110-38889-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate (B). MYPT1 was also immunoprecipitated by a previous lot (lot NB110-38889-1) of this antibody and by another rabbit anti-MYPT1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
quantity: 100 ul
price: 459 USD
to the supplier
domestic goat polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-75097] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic and some nuclear staining.
quantity: 100 ug
price: 399 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation