domestic sheep polyclonal
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
mouse monoclonal (4111)
application: immunohistochemistry, immunohistochemistry - paraffin section, dot blot
citations: 1
application: immunohistochemistry, immunohistochemistry - paraffin section, dot blot
citations: 1
domestic rabbit monoclonal (RM231)
application: ELISA, immunocytochemistry, flow cytometry, dot blot
application: ELISA, immunocytochemistry, flow cytometry, dot blot
Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Immunocytochemical staining of HeLa cells using Anti-5-MethylCytosine antibody (NBP2-61470) (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
quantity: 50 ug
price: 379 USD
to the supplier
mouse monoclonal (33D3)
application: immunocytochemistry, dot blot
application: immunocytochemistry, dot blot
Dot Blot: 5-MethylCytosine Antibody (33D3) [NBP2-54609] - [Ovalbumine] [NBP2-54609] - To demonstrate the specificity of the antibody against 5-mC, a Dot blot analysis was performed using hmC, mC and C controls. 100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane. The antibody was used at a dilution of 1:300. Figure shows a high specificity of the antibody for the methylated control.
quantity: 100 ug
price: 629 USD
to the supplier
mouse monoclonal (C.15200003)
application: immunocytochemistry, dot blot
application: immunocytochemistry, dot blot
Immunofluorescence: 5-MethylCytosine Antibody (C.15200003) [NBP2-59166] - To detect methylated chromosomal regions, FISH was performed on metaphase chromosomes from HeLa cells using the antibody against 5-mC. The cells were blocked in metaphase by treatment with colcemid (0.1 ug/mL) for 1 - 2 hours, fixed overnight at -20C with ethanol/glacial acetic acid and treated with 2N HCl for 30 minutes at room temperature. Subsequently, the cells were blocked with PBS containing 1% BSA and 0.1% Triton X-100 and stained with the 5-mC antibody (left) diluted 1:1,000 in blocking solution, followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the chromosomes with DAPI. A merge of the two stainings is shown on the right.
quantity: 50 ug
price: 399 USD
to the supplier