HMGB1/HMG-1 Antibody
Novus Biologicals
catalog: NB100-2322
rabbit polyclonal
reactivity: human, mouse, dog, cow
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 8

Flow Cytometry: HMGB1/HMG-1 Antibody [NB100-2322] - Staining of NTERA-2 cells using NB100-2322 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.

Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Detection of HMGB1 in whole cell HeLa lysate using NB100-2322. ECL exposure, 1 min.

Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody [NB100-2322] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HMGB1 [NB100-2322] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
quantity: 0.1 ml
price: 339 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [Alexa Fluor® 405]
Novus Biologicals
catalog: NB100-2322AF405
rabbit polyclonal
reactivity: human, dog, cow
conjugate: Alexa Fluor 405
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 0.1 ml
price: 369 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [Alexa Fluor® 488]
Novus Biologicals
catalog: NB100-2322AF488
rabbit polyclonal
reactivity: human, dog, cow
conjugate: AF488
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 0.1 ml
price: 369 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [Alexa Fluor® 647]
Novus Biologicals
catalog: NB100-2322AF647
rabbit polyclonal
reactivity: human, dog, cow
conjugate: AF647
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 0.1 ml
price: 369 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [Alexa Fluor® 700]
Novus Biologicals
catalog: NB100-2322AF700
rabbit polyclonal
reactivity: human, dog, cow
conjugate: AF700
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 0.1 ml
price: 369 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [Biotin]
Novus Biologicals
catalog: NB100-2322B
rabbit polyclonal
reactivity: human, dog, cow
conjugate: biotin
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 0.1 ml
price: 369 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [DyLight 650]
Novus Biologicals
catalog: NB100-2322C
rabbit polyclonal
reactivity: human, dog, cow
conjugate: DyLight 650
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 0.1 ml
price: 369 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [FITC]
Novus Biologicals
catalog: NB100-2322F
rabbit polyclonal
reactivity: human, dog, cow
conjugate: FITC
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 0.1 ml
price: 369 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [DyLight 488]
Novus Biologicals
catalog: NB100-2322G
rabbit polyclonal
reactivity: human, dog, cow
conjugate: DyLight488
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 0.1 ml
price: 369 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [HRP]
Novus Biologicals
catalog: NB100-2322H
rabbit polyclonal
reactivity: human, dog, cow
conjugate: HRP
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

ELISA: HMGB1/HMG-1 Antibody [HRP] [NB100-2322H] - A dose-dependent titration of the HRP conjugated anti-HMGB1 antibody on recombinant human HMGB1 protein. Image from verified customer review.
quantity: 0.1 ml
price: 399 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [PE]
Novus Biologicals
catalog: NB100-2322PE
rabbit polyclonal
reactivity: human, dog, cow
conjugate: PE
application: flow cytometry
quantity: 0.1 ml
price: 369 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [DyLight 550]
Novus Biologicals
catalog: NB100-2322R
rabbit polyclonal
reactivity: human, dog, cow
conjugate: DyLight 550
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 0.1 ml
price: 369 USD
to the supplier
Rabbit Polyclonal HMGB1/HMG-1 Antibody [DyLight 405]
Novus Biologicals
catalog: NB100-2322V
rabbit polyclonal
reactivity: human, dog, cow
conjugate: DyLight 405
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 0.1 ml
price: 369 USD
to the supplier
HMGB1/HMG-1 Antibody (1F3)
Novus Biologicals
catalog: NBP2-25148
mouse monoclonal (1F3)
reactivity: human, cow
application: western blot, immunocytochemistry, flow cytometry

Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - HeLa cells grown in tissue culture and stained with NBP2-25148 (red), chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25148 reveals strong nuclear staining which overlaps with the DNA stain.

Simple Western: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Simple Western lane view shows a specific band for HMGB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Flow Cytometry: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HMGB1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
quantity: 0.1 ml
price: 279 USD
to the supplier