gamma H2AX [p Ser139] Antibody
Novus Biologicals
catalog: NB100-384

rabbit polyclonal
reactivity: human, mouse, rat, dog
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 28

Simple Western: gamma H2AX [p Ser139] Antibody [NB100-384] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.

Immunohistochemistry-Paraffin: gamma H2AX [p Ser139] Antibody [NB100-384] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-gamma-H2AX used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.

Flow Cytometry: gamma H2AX [p Ser139] Antibody [NB100-384] - Analysis of gamma-H2AX in Etoposide Treated Jurkat Cells. Cells were treated for 3 hrs in 5ug/ml etoposide, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 0.5 ug anti-KLH or anti-H2AX NB100-384 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- etosposide treated, anti-KLH; Red- untreated, anti-gamma H2AX; Blue- etoposide treated , anti-gamma H2AX.
quantity: 0.1 ml
price: 399 USD
to the supplier

gamma H2AX [p Ser139] Antibody
Novus Biologicals
catalog: NB100-2280

rabbit polyclonal
reactivity: human, mouse, dog
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 6

Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-2280] - Sections of human colon adenocarcinoma. Mock treatment (left) or lambda phosphatase-treatment (right). Antibodies: Affinity purified rabbit anti-Gamma H2AX (NB100-2280) used at a dilution of 1:250. Detection: DAB

Immunocytochemistry/Immunofluorescence: gamma H2AX [p Ser139] Antibody [NB100-2280] - Neocarzinostatin treated asynchronous HeLa cells. Affinity purified rabbit anti-gamma-H2AX used at a dilution of 1:100. Detection: Red fluorescent Anti rabbit IgG-DyLight (r) 594 conjugated used at a dilution of 1:100.

Simple Western: gamma H2AX [p Ser139] Antibody [NB100-2280] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
quantity: 0.1 ml
price: 449 USD
to the supplier

Histone H2AX Antibody
Novus Biologicals
catalog: NB100-638

rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 5

Immunocytochemistry/Immunofluorescence: H2AX Antibody [NB100-638] - Asynchronous wild-type (+/+) and H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody used at 2 ug/ml.

Western Blot: H2AX Antibody [NB100-638] - Human 293T and wild-type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody used at the indicated concentrations.
quantity: 100 ul
price: 379 USD
to the supplier

Histone H2AX Antibody
Novus Biologicals
catalog: NB100-383

rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 1

Proximity Ligation Assay: H2AX Antibody [NB100-383] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human DMAP1 (NB100-256) and rabbit anti-human H2AX (NB100-383). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: H2AX Antibody [NB100-383] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human H2AFX (NB100-383). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: H2AX Antibody [NB100-383] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anit-human H2AX (NB100-383). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
quantity: 100 ul
price: 379 USD
to the supplier

gamma H2AX [p Ser139] Antibody (3F2)
Novus Biologicals
catalog: NB100-74435

mouse monoclonal (3F2)
reactivity: human, mouse, cow
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 4

Immunocytochemistry/Immunofluorescence: gamma H2AX [p Ser140] Antibody (3F2) [NB100-74435] - Staining using NB100-74435, treatment with paraquat and iron induces MnSOD and Phosphorylation of H2AX in RAW 264.7 macrophages. Cells were treated for 20 hours with paraquat (500 uM) and iron (200 ug/ml) and stained with anti-Phospho-H2AX antibody.

Immunocytochemistry/Immunofluorescence: gamma H2AX [p Ser140] Antibody (3F2) [NB100-74435] - Analysis of phosphorylated H2AX in gamma irradiated HeLa cells.

Western Blot: gamma H2AX [p Ser140] Antibody (3F2) [NB100-74435] - Figure 1 illustrates WB analysis of phosphorylated H2AX in gamma irradiated Hela cell lysate. Lane 1: Unphosphorylated H2AX control Lane 2: Phosphorylated H2AX. Figure 2 shows an IF image of phosphorylated H2AX in gamma irradiated Hela cells.
quantity: 100 ug
price: 349 USD
to the supplier

Histone H2AX Antibody
Novus Biologicals
catalog: NBP2-27428

rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Western Blot: H2AX Antibody [NBP2-27428] - Analysis of MOLT4 lysate in the 1) absence and 2) presence of immunizing peptide using Histone H2A.x antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: H2AX Antibody [NBP2-27428] - Formalin-fixed, paraffin-embedded human spleen stained with Histone H2A.x antibody at 10 ug/ml.
quantity: 0.1 mg (also 0.025 mg)
price: 279 USD
to the supplier

gamma H2AX [p Ser139] Antibody (9F3)
Novus Biologicals
catalog: NBP1-19255

mouse monoclonal (9F3)
reactivity: human, mouse, rat, dog, chicken, cow
application: western blot
citations: 1

Western Blot: gamma H2AX [p Ser139] Antibody (9F3) [NBP1-19255] - Lane 1: MWM, Lane 2: Jurkak, Lane 3: Jurkat staurosporine, Lane 4: 3T3, Lane 5: CHO-K1, Lane 6: Rat-2
quantity: 0.05 mg (also 0.2 mg)
price: 279 USD
to the supplier