EGLN3/PHD3 Antibody
Novus Biologicals
catalog: NB100-139

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 26

Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [NB100-139] - Analysis of a formalin fixed tissue section of human renal cell carcinoma (clear cell type) using rabbit polyclonal EGLN3/PHD3 antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong nuclear staining of PHD3 primarily in the cancer cells while the stromal cells were largely negative for this protein.

Immunocytochemistry/Immunofluorescence: EGLN3/PHD3 Antibody [NB100-139] - EGLN3/PHD3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western Blot: EGLN3/PHD3 Antibody [NB100-139] - Detection of PHD3 by Western Blot application in lysate from Rat cells before or 9 hours after inducing hypoxia. Primary antibody PHD3 NB100-139 was used at a dilution of 1:5000 and blots were developed using chemiluminescence.
quantity: 0.1 ml
price: 329 USD
to the supplier

Rabbit Polyclonal EGLN3/PHD3 Antibody [Alexa Fluor® 405]
Novus Biologicals
catalog: NB100-139AF405

rabbit polyclonal
reactivity: human, mouse, rat
conjugate: Alexa Fluor 405
application: western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 0.1 ml
price: 369 USD
to the supplier

Rabbit Polyclonal EGLN3/PHD3 Antibody [Alexa Fluor® 488]
Novus Biologicals
catalog: NB100-139AF488

rabbit polyclonal
reactivity: human, mouse, rat
conjugate: AF488
application: western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 0.1 ml
price: 369 USD
to the supplier

Rabbit Polyclonal EGLN3/PHD3 Antibody [Alexa Fluor® 647]
Novus Biologicals
catalog: NB100-139AF647

rabbit polyclonal
reactivity: human, mouse, rat
conjugate: AF647
application: western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 0.1 ml
price: 369 USD
to the supplier

Rabbit Polyclonal EGLN3/PHD3 Antibody [Alexa Fluor® 700]
Novus Biologicals
catalog: NB100-139AF700

rabbit polyclonal
reactivity: human, mouse, rat
conjugate: AF700
application: western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 0.1 ml
price: 369 USD
to the supplier

Rabbit Polyclonal EGLN3/PHD3 Antibody [DyLight 405]
Novus Biologicals
catalog: NB100-139V

rabbit polyclonal
reactivity: human, mouse, rat
conjugate: DyLight 405
application: western blot, immunocytochemistry

quantity: 0.1 ml
price: 369 USD
to the supplier

EGLN3/PHD3 Antibody
Novus Biologicals
catalog: NB100-303

rabbit polyclonal
reactivity: primates, human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 30

Western Blot: EGLN3/PHD3 Antibody [NB100-303] - Total protein from Mouse MEF, Human RT4 and HeLa, and Rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-PHD3 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: EGLN3/PHD3 Antibody [NB100-303] - analysis of PHD3 in ARPE-19 cells using anti-PHD3 antibody. Image from verified customer review.

Western Blot: EGLN3/PHD3 Antibody [NB100-303] - Whole cell protein from MEF cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-EGLN3/PHD3 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
quantity: 0.1 ml
price: 349 USD
to the supplier