product summary
company name :
Neuromab
product type :
antibody
product name :
GluN2B/NR2B glutamate receptor
catalog :
75-097
quantity :
100 ug
price :
269 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
N59/20
reactivity :
mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, western blot knockout validation
more info or order :
citations: 66
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 2a
Koster K, Francesconi W, Berton F, Alahmadi S, Srinivas R, Yoshii A. Developmental NMDA receptor dysregulation in the infantile neuronal ceroid lipofuscinosis mouse model. elife. 2019;8: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s6c
Wang Y, Figueiredo D, Sun X, Dong Z, Chen W, Cui W, et al. Controlling of glutamate release by neuregulin3 via inhibiting the assembly of the SNARE complex. Proc Natl Acad Sci U S A. 2018;115:2508-2513 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2a
Wilkinson B, Li J, Coba M. Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Sci Rep. 2017;7:5272 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 1d
Gross G, Straub C, Perez Sanchez J, Dempsey W, Junge J, Roberts R, et al. An E3-ligase-based method for ablating inhibitory synapses. Nat Methods. 2016;13:673-8 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4e
Yang P, Leu D, Ye K, Srinivasan C, Fike J, Huang T. Cognitive impairments following cranial irradiation can be mitigated by treatment with a tropomyosin receptor kinase B agonist. Exp Neurol. 2016;279:178-186 pubmed publisher
  • western blot; mouse; fig 4b
Kim S, Kim T, Lee H, Kong Y, Kaang B. Mind Bomb-2 Regulates Hippocampus-dependent Memory Formation and Synaptic Plasticity. Korean J Physiol Pharmacol. 2015;19:515-22 pubmed publisher
  • western blot; mouse; 1:2000; fig 2
McGuier N, Padula A, Mulholland P, Chandler L. Homer2 deletion alters dendritic spine morphology but not alcohol-associated adaptations in GluN2B-containing N-methyl-D-aspartate receptors in the nucleus accumbens. Front Pharmacol. 2015;6:28 pubmed publisher
  • western blot; mouse; fig 7
Lo S, Wang Y, Weber M, Larson J, Scearce Levie K, Sheng M. Caspase-3 deficiency results in disrupted synaptic homeostasis and impaired attention control. J Neurosci. 2015;35:2118-32 pubmed publisher
  • western blot; mouse; 1:1000
Portugal G, Al Hasani R, Fakira A, Gonzalez Romero J, Melyan Z, McCall J, et al. Hippocampal long-term potentiation is disrupted during expression and extinction but is restored after reinstatement of morphine place preference. J Neurosci. 2014;34:527-38 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:300
  • western blot; mouse; 1:1000
Hoy J, Haeger P, Constable J, Arias R, McCallum R, Kyweriga M, et al. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions. J Neurosci. 2013;33:9364-84 pubmed publisher
  • western blot; rat
Murata Y, Constantine Paton M. Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway. J Neurosci. 2013;33:5040-52 pubmed publisher
Gajardo I, Salazar C, Lopez Espíndola D, Estay C, Flores Muñoz C, Elgueta C, et al. Lack of Pannexin 1 Alters Synaptic GluN2 Subunit Composition and Spatial Reversal Learning in Mice. Front Mol Neurosci. 2018;11:114 pubmed publisher
Berg J, Lee C, Chen L, Galvan L, Cepeda C, Chen J, et al. JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse. Neuron. 2015;88:1173-1191 pubmed publisher
Zollinger D, Chang K, Baalman K, Kim S, Rasband M. The Polarity Protein Pals1 Regulates Radial Sorting of Axons. J Neurosci. 2015;35:10474-84 pubmed publisher
Chen N, Koopmans F, Gordon A, Paliukhovich I, Klaassen R, van der Schors R, et al. Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes. Biochim Biophys Acta. 2015;1854:827-33 pubmed publisher
Chassefeyre R, Martínez Hernández J, Bertaso F, Bouquier N, Blot B, Laporte M, et al. Regulation of postsynaptic function by the dementia-related ESCRT-III subunit CHMP2B. J Neurosci. 2015;35:3155-73 pubmed publisher
D Orsi B, Kilbride S, Chen G, Perez Alvarez S, Bonner H, Pfeiffer S, et al. Bax regulates neuronal Ca2+ homeostasis. J Neurosci. 2015;35:1706-22 pubmed publisher
Neuner S, Wilmott L, Hope K, Hoffmann B, Chong J, Abramowitz J, et al. TRPC3 channels critically regulate hippocampal excitability and contextual fear memory. Behav Brain Res. 2015;281:69-77 pubmed publisher
Stroebel D, Carvalho S, Grand T, Zhu S, Paoletti P. Controlling NMDA receptor subunit composition using ectopic retention signals. J Neurosci. 2014;34:16630-6 pubmed publisher
McGuier N, Padula A, Lopez M, Woodward J, Mulholland P. Withdrawal from chronic intermittent alcohol exposure increases dendritic spine density in the lateral orbitofrontal cortex of mice. Alcohol. 2015;49:21-7 pubmed publisher
de Andrade G, Kunzelman L, Merrill M, Fuerst P. Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina. Mol Vis. 2014;20:1422-33 pubmed
Mellone M, Pelucchi S, Alberti L, Genazzani A, Di Luca M, Gardoni F. Zinc transporter-1: a novel NMDA receptor-binding protein at the postsynaptic density. J Neurochem. 2015;132:159-68 pubmed publisher
Bhattacharyya M, Battacharya M, Nandanoor A, Osman M, Kasinathan C, FREDERIKSE P. NMDA glutamate receptor NR1, NR2A and NR2B expression and NR2B Tyr-1472 phosphorylation in the lens. Neurochem Res. 2014;39:1825-32 pubmed publisher
Kehoe L, Bellone C, De Roo M, Zandueta A, Dey P, Pérez Otaño I, et al. GluN3A promotes dendritic spine pruning and destabilization during postnatal development. J Neurosci. 2014;34:9213-21 pubmed publisher
Neumann J, Dash Wagh S, Jüngling K, Tsai T, Meschkat M, Räk A, et al. Y-P30 promotes axonal growth by stabilizing growth cones. Brain Struct Funct. 2015;220:1935-50 pubmed publisher
Trantham Davidson H, Burnett E, Gass J, Lopez M, Mulholland P, Centanni S, et al. Chronic alcohol disrupts dopamine receptor activity and the cognitive function of the medial prefrontal cortex. J Neurosci. 2014;34:3706-18 pubmed publisher
Li Q, Zheng S, Han A, Lin C, Stoilov P, Fu X, et al. The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation. elife. 2014;3:e01201 pubmed publisher
Gunn B, Cunningham L, Cooper M, Corteen N, Seifi M, Swinny J, et al. Dysfunctional astrocytic and synaptic regulation of hypothalamic glutamatergic transmission in a mouse model of early-life adversity: relevance to neurosteroids and programming of the stress response. J Neurosci. 2013;33:19534-54 pubmed publisher
Fiuza M, González González I, Pérez Otaño I. GluN3A expression restricts spine maturation via inhibition of GIT1/Rac1 signaling. Proc Natl Acad Sci U S A. 2013;110:20807-12 pubmed publisher
Kunda S, Cheriyan J, Hur M, Balsara R, Castellino F. Antagonist properties of Conus parius peptides on N-methyl-D-aspartate receptors and their effects on CREB signaling. PLoS ONE. 2013;8:e81405 pubmed publisher
den Hartog C, Beckley J, Smothers T, Lench D, Holseberg Z, Fedarovich H, et al. Alterations in ethanol-induced behaviors and consumption in knock-in mice expressing ethanol-resistant NMDA receptors. PLoS ONE. 2013;8:e80541 pubmed publisher
Yuan T, Mameli M, O Connor E, Dey P, Verpelli C, Sala C, et al. Expression of cocaine-evoked synaptic plasticity by GluN3A-containing NMDA receptors. Neuron. 2013;80:1025-38 pubmed publisher
Breiderhoff T, Christiansen G, Pallesen L, Vaegter C, Nykjaer A, Holm M, et al. Sortilin-related receptor SORCS3 is a postsynaptic modulator of synaptic depression and fear extinction. PLoS ONE. 2013;8:e75006 pubmed publisher
Krüger J, Favaro P, Liu M, Kitlinska A, Huang X, Raabe M, et al. Differential roles of postsynaptic density-93 isoforms in regulating synaptic transmission. J Neurosci. 2013;33:15504-17 pubmed publisher
Viviani B, Boraso M, Valero M, Gardoni F, Marco E, Llorente R, et al. Early maternal deprivation immunologically primes hippocampal synapses by redistributing interleukin-1 receptor type I in a sex dependent manner. Brain Behav Immun. 2014;35:135-43 pubmed publisher
Barcomb K, Coultrap S, Bayer K. Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B. Mol Pharmacol. 2013;84:834-43 pubmed publisher
Gaier E, Miller M, Ralle M, Aryal D, Wetsel W, Mains R, et al. Peptidylglycine α-amidating monooxygenase heterozygosity alters brain copper handling with region specificity. J Neurochem. 2013;127:605-19 pubmed publisher
Bonnet S, Akad D, Samaddar T, Liu Y, Huang X, Dong Y, et al. Synaptic state-dependent functional interplay between postsynaptic density-95 and synapse-associated protein 102. J Neurosci. 2013;33:13398-409 pubmed publisher
Frühbeis C, Fröhlich D, Kuo W, Amphornrat J, Thilemann S, Saab A, et al. Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication. PLoS Biol. 2013;11:e1001604 pubmed publisher
Marco S, Giralt A, Petrovic M, Pouladi M, Mart nez Turrillas R, Mart nez Hern ndez J, et al. Suppressing aberrant GluN3A expression rescues synaptic and behavioral impairments in Huntington's disease models. Nat Med. 2013;19:1030-8 pubmed publisher
Brigman J, Daut R, Wright T, Gunduz Cinar O, Graybeal C, Davis M, et al. GluN2B in corticostriatal circuits governs choice learning and choice shifting. Nat Neurosci. 2013;16:1101-10 pubmed publisher
Chowdhury D, Marco S, Brooks I, Zandueta A, Rao Y, Haucke V, et al. Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors. J Neurosci. 2013;33:4151-64 pubmed publisher
Di Filippo M, Chiasserini D, Gardoni F, Viviani B, Tozzi A, Giampà C, et al. Effects of central and peripheral inflammation on hippocampal synaptic plasticity. Neurobiol Dis. 2013;52:229-36 pubmed publisher
Bagetta V, Sgobio C, Pendolino V, Del Papa G, Tozzi A, Ghiglieri V, et al. Rebalance of striatal NMDA/AMPA receptor ratio underlies the reduced emergence of dyskinesia during D2-like dopamine agonist treatment in experimental Parkinson's disease. J Neurosci. 2012;32:17921-31 pubmed publisher
Harraz M, Eacker S, Wang X, Dawson T, Dawson V. MicroRNA-223 is neuroprotective by targeting glutamate receptors. Proc Natl Acad Sci U S A. 2012;109:18962-7 pubmed publisher
Mazzone C, Larese T, Kiraly D, Eipper B, Mains R. Analysis of kalirin-7 knockout mice reveals different effects in female mice. Mol Pharmacol. 2012;82:1241-9 pubmed publisher
Zhang Y, Xiao X, Zhang X, Zhao Z, Zhang Y. Estrogen facilitates spinal cord synaptic transmission via membrane-bound estrogen receptors: implications for pain hypersensitivity. J Biol Chem. 2012;287:33268-81 pubmed
Xiao X, Yang Y, Zhang Y, Zhang X, Zhao Z, Zhang Y. Estrogen in the anterior cingulate cortex contributes to pain-related aversion. Cereb Cortex. 2013;23:2190-203 pubmed publisher
Mulholland P, Jordan B, Chandler L. Chronic ethanol up-regulates the synaptic expression of the nuclear translational regulatory protein AIDA-1 in primary hippocampal neurons. Alcohol. 2012;46:569-76 pubmed publisher
Wilke S, Hall B, Antonios J, DeNardo L, Otto S, Yuan B, et al. NeuroD2 regulates the development of hippocampal mossy fiber synapses. Neural Dev. 2012;7:9 pubmed publisher
Wang C, Held R, Chang S, Yang L, Delpire E, Ghosh A, et al. A critical role for GluN2B-containing NMDA receptors in cortical development and function. Neuron. 2011;72:789-805 pubmed publisher
Peng X, Kim J, Zhou Z, Fink D, Mata M. Neuronal Nogo-A regulates glutamate receptor subunit expression in hippocampal neurons. J Neurochem. 2011;119:1183-93 pubmed publisher
Kiraly D, Lemtiri Chlieh F, Levine E, Mains R, Eipper B. Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function. J Neurosci. 2011;31:12554-65 pubmed publisher
Eipper Mains J, Kiraly D, Palakodeti D, Mains R, Eipper B, Graveley B. microRNA-Seq reveals cocaine-regulated expression of striatal microRNAs. RNA. 2011;17:1529-43 pubmed publisher
Doherty F, Sladek C. NMDA receptor subunit expression in the supraoptic nucleus of adult rats: dominance of NR2B and NR2D. Brain Res. 2011;1388:89-99 pubmed publisher
Mains R, Kiraly D, Eipper Mains J, Ma X, Eipper B. Kalrn promoter usage and isoform expression respond to chronic cocaine exposure. BMC Neurosci. 2011;12:20 pubmed publisher
Fourgeaud L, Davenport C, Tyler C, Cheng T, Spencer M, Boulanger L. MHC class I modulates NMDA receptor function and AMPA receptor trafficking. Proc Natl Acad Sci U S A. 2010;107:22278-83 pubmed publisher
Hsin H, Kim M, Wang C, Sheng M. Proline-rich tyrosine kinase 2 regulates hippocampal long-term depression. J Neurosci. 2010;30:11983-93 pubmed publisher
Antion M, Christie L, Bond A, Dalva M, Contractor A. Ephrin-B3 regulates glutamate receptor signaling at hippocampal synapses. Mol Cell Neurosci. 2010;45:378-88 pubmed publisher
Perez de Arce K, de Arce K, Varela Nallar L, Farias O, Cifuentes A, Bull P, et al. Synaptic clustering of PSD-95 is regulated by c-Abl through tyrosine phosphorylation. J Neurosci. 2010;30:3728-38 pubmed publisher
Edbauer D, Neilson J, Foster K, Wang C, Seeburg D, Batterton M, et al. Regulation of synaptic structure and function by FMRP-associated microRNAs miR-125b and miR-132. Neuron. 2010;65:373-84 pubmed publisher
Longordo F, Kopp C, Mishina M, Lujan R, Lüthi A. NR2A at CA1 synapses is obligatory for the susceptibility of hippocampal plasticity to sleep loss. J Neurosci. 2009;29:9026-41 pubmed publisher
Gardoni F, Mauceri D, Malinverno M, Polli F, Costa C, Tozzi A, et al. Decreased NR2B subunit synaptic levels cause impaired long-term potentiation but not long-term depression. J Neurosci. 2009;29:669-77 pubmed publisher
Ghasemzadeh M, Vasudevan P, Mueller C. Locomotor sensitization to cocaine is associated with distinct pattern of glutamate receptor trafficking to the postsynaptic density in prefrontal cortex: early versus late withdrawal effects. Pharmacol Biochem Behav. 2009;92:383-92 pubmed publisher
Ghasemzadeh M, Mueller C, Vasudevan P. Behavioral sensitization to cocaine is associated with increased glutamate receptor trafficking to the postsynaptic density after extended withdrawal period. Neuroscience. 2009;159:414-26 pubmed publisher
Ma X, Kiraly D, Gaier E, Wang Y, Kim E, Levine E, et al. Kalirin-7 is required for synaptic structure and function. J Neurosci. 2008;28:12368-82 pubmed publisher
product information
TargetType :
Receptors
Target :
GluN2B/NR2B glutamate receptor
AccessionNum :
Q00960
Clone :
N59/20
HumanGeneName :
GRIN2B
DataSheetFileName :
neuromab.ucdavis.edu/datasheet/N59_20.pdf
IsoType :
IgG2a
ValidationT :
Pass
ValidationBrIB :
Pass
ValidationBrIHC :
Pass
ValidationKO :
Pass
Catalog :
75-097
more info or order :
company information
Neuromab
PO Box 1560 Davis, CA 95617-1560
http://neuromab.ucdavis.edu
headquarters: United States