product summary
company name :
Neuromab
product type :
antibody
product name :
Slo1/BKAlpha maxi-K+ channel
catalog :
73-022
quantity :
5 ml
price :
319 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
L6/60
reactivity :
mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation
more info or order :
citations: 71
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; mouse; fig 2
  • western blot; mouse
Martinez Espinosa P, Yang C, González Pérez V, Xia X, Lingle C. Knockout of the BK β2 subunit abolishes inactivation of BK currents in mouse adrenal chromaffin cells and results in slow-wave burst activity. J Gen Physiol. 2014;144:275-95 pubmed publisher
Whitt J, Montgomery J, Meredith A. BK channel inactivation gates daytime excitability in the circadian clock. Nat Commun. 2016;7:10837 pubmed publisher
Singh H, Li M, Hall L, Chen S, Sukur S, Lu R, et al. MaxiK channel interactome reveals its interaction with GABA transporter 3 and heat shock protein 60 in the mammalian brain. Neuroscience. 2016;317:76-107 pubmed publisher
Manzanares D, Krick S, Baumlin N, Dennis J, Tyrrell J, Tarran R, et al. Airway Surface Dehydration by Transforming Growth Factor β (TGF-β) in Cystic Fibrosis Is Due to Decreased Function of a Voltage-dependent Potassium Channel and Can Be Rescued by the Drug Pirfenidone. J Biol Chem. 2015;290:25710-6 pubmed publisher
Pratt C, He J, Wang Y, Barth A, Bruchez M. Fluorogenic Green-Inside Red-Outside (GIRO) Labeling Approach Reveals Adenylyl Cyclase-Dependent Control of BKα Surface Expression. Bioconjug Chem. 2015;26:1963-71 pubmed publisher
Dell Orco J, Wasserman A, Chopra R, Ingram M, Hu Y, Singh V, et al. Neuronal Atrophy Early in Degenerative Ataxia Is a Compensatory Mechanism to Regulate Membrane Excitability. J Neurosci. 2015;35:11292-307 pubmed publisher
Zhao G, Li T, Brochet D, Rosenberg P, Lederer W. STIM1 enhances SR Ca2+ content through binding phospholamban in rat ventricular myocytes. Proc Natl Acad Sci U S A. 2015;112:E4792-801 pubmed publisher
Liu Y, Huang X, He X, Zhou Y, Jiang X, Chen Kiang S, et al. A novel effect of thalidomide and its analogs: suppression of cereblon ubiquitination enhances ubiquitin ligase function. FASEB J. 2015;29:4829-39 pubmed publisher
Leo M, Bulley S, Bannister J, Kuruvilla K, Narayanan D, Jaggar J. Angiotensin II stimulates internalization and degradation of arterial myocyte plasma membrane BK channels to induce vasoconstriction. Am J Physiol Cell Physiol. 2015;309:C392-402 pubmed publisher
Hirono M, Ogawa Y, Misono K, Zollinger D, Trimmer J, Rasband M, et al. BK Channels Localize to the Paranodal Junction and Regulate Action Potentials in Myelinated Axons of Cerebellar Purkinje Cells. J Neurosci. 2015;35:7082-94 pubmed publisher
White R, Zemen B, Khan Z, Montgomery J, Herrera G, Meredith A. Evaluation of mouse urinary bladder smooth muscle for diurnal differences in contractile properties. Front Pharmacol. 2014;5:293 pubmed publisher
Cornelius R, Wen D, Li H, Yuan Y, Wang France J, Warner P, et al. Low Na, high K diet and the role of aldosterone in BK-mediated K excretion. PLoS ONE. 2015;10:e0115515 pubmed publisher
Tanner M, Hu X, Huq R, Tajhya R, Sun L, Khan F, et al. KCa1.1 inhibition attenuates fibroblast-like synoviocyte invasiveness and ameliorates disease in rat models of rheumatoid arthritis. Arthritis Rheumatol. 2015;67:96-106 pubmed publisher
Zhang Z, Li M, Lu R, Alioua A, Stefani E, Toro L. The angiotensin II type 1 receptor (AT1R) closely interacts with large conductance voltage- and Ca2+-activated K+ (BK) channels and inhibits their activity independent of G-protein activation. J Biol Chem. 2014;289:25678-89 pubmed publisher
Nourian Z, Li M, Leo M, Jaggar J, Braun A, Hill M. Large conductance Ca2+-activated K+ channel (BKCa) ?-subunit splice variants in resistance arteries from rat cerebral and skeletal muscle vasculature. PLoS ONE. 2014;9:e98863 pubmed publisher
Li Y, Lorca R, Ma X, Rhodes A, England S. BK channels regulate myometrial contraction by modulating nuclear translocation of NF-?B. Endocrinology. 2014;155:3112-22 pubmed publisher
Hayoz S, Bradley V, Boerman E, Nourian Z, Segal S, Jackson W. Aging increases capacitance and spontaneous transient outward current amplitude of smooth muscle cells from murine superior epigastric arteries. Am J Physiol Heart Circ Physiol. 2014;306:H1512-24 pubmed publisher
Samengo I, Currò D, Barrese V, Taglialatela M, Martire M. Large conductance calcium-activated potassium channels: their expression and modulation of glutamate release from nerve terminals isolated from rat trigeminal caudal nucleus and cerebral cortex. Neurochem Res. 2014;39:901-10 pubmed publisher
Yi F, Wang H, Chai Q, Wang X, Shen W, Willis M, et al. Regulation of large conductance Ca2+-activated K+ (BK) channel ?1 subunit expression by muscle RING finger protein 1 in diabetic vessels. J Biol Chem. 2014;289:10853-64 pubmed publisher
Leo M, Bannister J, Narayanan D, Nair A, Grubbs J, Gabrick K, et al. Dynamic regulation of ?1 subunit trafficking controls vascular contractility. Proc Natl Acad Sci U S A. 2014;111:2361-6 pubmed publisher
Lu R, Lukowski R, Sausbier M, Zhang D, Sisignano M, Schuh C, et al. BKCa channels expressed in sensory neurons modulate inflammatory pain in mice. Pain. 2014;155:556-65 pubmed publisher
Mannowetz N, Naidoo N, Choo S, Smith J, Lishko P. Slo1 is the principal potassium channel of human spermatozoa. elife. 2013;2:e01009 pubmed publisher
van der Lubbe N, Moes A, Rosenbaek L, Schoep S, Meima M, Danser A, et al. K+-induced natriuresis is preserved during Na+ depletion and accompanied by inhibition of the Na+-Cl- cotransporter. Am J Physiol Renal Physiol. 2013;305:F1177-88 pubmed publisher
Wen D, Cornelius R, Yuan Y, Sansom S. Regulation of BK-? expression in the distal nephron by aldosterone and urine pH. Am J Physiol Renal Physiol. 2013;305:F463-76 pubmed publisher
Li M, Zhang Z, Koh H, Lu R, Jiang Z, Alioua A, et al. The ?1-subunit of the MaxiK channel associates with the thromboxane A2 receptor and reduces thromboxane A2 functional effects. J Biol Chem. 2013;288:3668-77 pubmed publisher
Montgomery J, Whitt J, Wright B, Lai M, Meredith A. Mis-expression of the BK K(+) channel disrupts suprachiasmatic nucleus circuit rhythmicity and alters clock-controlled behavior. Am J Physiol Cell Physiol. 2013;304:C299-311 pubmed publisher
Montgomery J, Meredith A. Genetic activation of BK currents in vivo generates bidirectional effects on neuronal excitability. Proc Natl Acad Sci U S A. 2012;109:18997-9002 pubmed publisher
Zhang J, Halm S, Halm D. Role of the BK channel (KCa1.1) during activation of electrogenic K+ secretion in guinea pig distal colon. Am J Physiol Gastrointest Liver Physiol. 2012;303:G1322-34 pubmed publisher
McFerrin M, Turner K, Cuddapah V, Sontheimer H. Differential role of IK and BK potassium channels as mediators of intrinsic and extrinsic apoptotic cell death. Am J Physiol Cell Physiol. 2012;303:C1070-8 pubmed publisher
Foller M, Jaumann M, Dettling J, Saxena A, Pakladok T, Munoz C, et al. AMP-activated protein kinase in BK-channel regulation and protection against hearing loss following acoustic overstimulation. FASEB J. 2012;26:4243-53 pubmed publisher
Manning C, Bundros A, Trimmer J. Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance. PLoS ONE. 2012;7:e38313 pubmed publisher
Menegola M, Clark E, Trimmer J. The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice. Epilepsia. 2012;53 Suppl 1:142-9 pubmed publisher
Zhang X, Mok L, Lee K, Charbonnet M, Gold M. Inflammation-induced changes in BK(Ca) currents in cutaneous dorsal root ganglion neurons from the adult rat. Mol Pain. 2012;8:37 pubmed publisher
Tanimoto N, Sothilingam V, Euler T, Ruth P, Seeliger M, Schubert T. BK channels mediate pathway-specific modulation of visual signals in the in vivo mouse retina. J Neurosci. 2012;32:4861-6 pubmed publisher
Shruti S, Urban Ciecko J, Fitzpatrick J, Brenner R, Bruchez M, Barth A. The brain-specific Beta4 subunit downregulates BK channel cell surface expression. PLoS ONE. 2012;7:e33429 pubmed publisher
Almassy J, Won J, BEGENISICH T, Yule D. Apical Ca2+-activated potassium channels in mouse parotid acinar cells. J Gen Physiol. 2012;139:121-33 pubmed publisher
Zhang D, Borbouse L, Gebremedhin D, Mendoza S, Zinkevich N, Li R, et al. H2O2-induced dilation in human coronary arterioles: role of protein kinase G dimerization and large-conductance Ca2+-activated K+ channel activation. Circ Res. 2012;110:471-80 pubmed publisher
Hu X, Laragione T, Sun L, Koshy S, Jones K, Ismailov I, et al. KCa1.1 potassium channels regulate key proinflammatory and invasive properties of fibroblast-like synoviocytes in rheumatoid arthritis. J Biol Chem. 2012;287:4014-22 pubmed publisher
Soloff M, Jeng Y, Izban M, Sinha M, Luxon B, Stamnes S, et al. Effects of progesterone treatment on expression of genes involved in uterine quiescence. Reprod Sci. 2011;18:781-97 pubmed publisher
Wykes R, Kalmbach A, Eliava M, Waters J. Changes in the physiology of CA1 hippocampal pyramidal neurons in preplaque CRND8 mice. Neurobiol Aging. 2012;33:1609-23 pubmed publisher
McDermott C, Schrader L. Activation of ? opioid receptors increases intrinsic excitability of dentate gyrus granule cells. J Physiol. 2011;589:3517-32 pubmed publisher
Li B, Glazebrook P, Kunze D, Schild J. KCa1.1 channel contributes to cell excitability in unmyelinated but not myelinated rat vagal afferents. Am J Physiol Cell Physiol. 2011;300:C1393-403 pubmed publisher
Tajima N, Itokazu Y, Korpi E, Somerharju P, Käkelä R. Activity of BK(Ca) channel is modulated by membrane cholesterol content and association with Na+/K+-ATPase in human melanoma IGR39 cells. J Biol Chem. 2011;286:5624-38 pubmed publisher
Wersinger E, McLean W, Fuchs P, Pyott S. BK channels mediate cholinergic inhibition of high frequency cochlear hair cells. PLoS ONE. 2010;5:e13836 pubmed publisher
Mazar J, DeYoung K, Khaitan D, Meister E, Almodovar A, Goydos J, et al. The regulation of miRNA-211 expression and its role in melanoma cell invasiveness. PLoS ONE. 2010;5:e13779 pubmed publisher
Li M, Tanaka Y, Alioua A, Wu Y, Lu R, Kundu P, et al. Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation. Proc Natl Acad Sci U S A. 2010;107:19096-101 pubmed publisher
Ernest N, Logsdon N, McFerrin M, Sontheimer H, Spiller S. Biophysical properties of human medulloblastoma cells. J Membr Biol. 2010;237:59-69 pubmed publisher
Zhao G, Neeb Z, Leo M, Pachuau J, Adebiyi A, Ouyang K, et al. Type 1 IP3 receptors activate BKCa channels via local molecular coupling in arterial smooth muscle cells. J Gen Physiol. 2010;136:283-91 pubmed publisher
Martire M, Barrese V, D Amico M, Iannotti F, Pizzarelli R, Samengo I, et al. Pre-synaptic BK channels selectively control glutamate versus GABA release from cortical and hippocampal nerve terminals. J Neurochem. 2010;115:411-22 pubmed publisher
Kim E, Suh J, Chiu Y, Dryer S. Regulation of podocyte BK(Ca) channels by synaptopodin, Rho, and actin microfilaments. Am J Physiol Renal Physiol. 2010;299:F594-604 pubmed publisher
Yan J, Aldrich R. LRRC26 auxiliary protein allows BK channel activation at resting voltage without calcium. Nature. 2010;466:513-6 pubmed publisher
Kaufmann W, Kasugai Y, Ferraguti F, Storm J. Two distinct pools of large-conductance calcium-activated potassium channels in the somatic plasma membrane of central principal neurons. Neuroscience. 2010;169:974-86 pubmed publisher
Holtzclaw J, Liu L, Grimm P, Sansom S. Shear stress-induced volume decrease in C11-MDCK cells by BK-alpha/beta4. Am J Physiol Renal Physiol. 2010;299:F507-16 pubmed publisher
Chen X, Kovalchuk Y, Adelsberger H, Henning H, Sausbier M, Wietzorrek G, et al. Disruption of the olivo-cerebellar circuit by Purkinje neuron-specific ablation of BK channels. Proc Natl Acad Sci U S A. 2010;107:12323-8 pubmed publisher
Holtzclaw J, Grimm P, Sansom S. Intercalated cell BK-alpha/beta4 channels modulate sodium and potassium handling during potassium adaptation. J Am Soc Nephrol. 2010;21:634-45 pubmed publisher
Kim E, Chiu Y, Dryer S. Neph1 regulates steady-state surface expression of Slo1 Ca(2+)-activated K(+) channels: different effects in embryonic neurons and podocytes. Am J Physiol Cell Physiol. 2009;297:C1379-88 pubmed publisher
Schweizer F, Savin D, Luu C, Sultemeier D, Hoffman L. Distribution of high-conductance calcium-activated potassium channels in rat vestibular epithelia. J Comp Neurol. 2009;517:134-45 pubmed publisher
Sokolowski B, Duncan R, Chen S, Karolat J, Kathiresan T, Harvey M. The large-conductance Ca(2+)-activated K(+) channel interacts with the apolipoprotein ApoA1. Biochem Biophys Res Commun. 2009;387:671-5 pubmed publisher
Yang C, Zeng X, Xia X, Lingle C. Interactions between beta subunits of the KCNMB family and Slo3: beta4 selectively modulates Slo3 expression and function. PLoS ONE. 2009;4:e6135 pubmed publisher
Grimm P, Irsik D, Liu L, Holtzclaw J, Sansom S. Role of BKbeta1 in Na+ reabsorption by cortical collecting ducts of Na+-deprived mice. Am J Physiol Renal Physiol. 2009;297:F420-8 pubmed publisher
Kaufmann W, Ferraguti F, Fukazawa Y, Kasugai Y, Shigemoto R, Laake P, et al. Large-conductance calcium-activated potassium channels in purkinje cell plasma membranes are clustered at sites of hypolemmal microdomains. J Comp Neurol. 2009;515:215-30 pubmed publisher
Ridgway L, Kim E, Dryer S. MAGI-1 interacts with Slo1 channel proteins and suppresses Slo1 expression on the cell surface. Am J Physiol Cell Physiol. 2009;297:C55-65 pubmed publisher
Kim E, Alvarez Baron C, Dryer S. Canonical transient receptor potential channel (TRPC)3 and TRPC6 associate with large-conductance Ca2+-activated K+ (BKCa) channels: role in BKCa trafficking to the surface of cultured podocytes. Mol Pharmacol. 2009;75:466-77 pubmed publisher
Buniel M, Glazebrook P, Ramirez Navarro A, Kunze D. Distribution of voltage-gated potassium and hyperpolarization-activated channels in sensory afferent fibers in the rat carotid body. J Comp Neurol. 2008;510:367-77 pubmed publisher
Yan J, Olsen J, Park K, Li W, Bildl W, Schulte U, et al. Profiling the phospho-status of the BKCa channel alpha subunit in rat brain reveals unexpected patterns and complexity. Mol Cell Proteomics. 2008;7:2188-98 pubmed publisher
Kim E, Choi K, Dryer S. Nephrin binds to the COOH terminus of a large-conductance Ca2+-activated K+ channel isoform and regulates its expression on the cell surface. Am J Physiol Renal Physiol. 2008;295:F235-46 pubmed publisher
Levy D, Wanderling S, Biemesderfer D, Goldstein S. MiRP3 acts as an accessory subunit with the BK potassium channel. Am J Physiol Renal Physiol. 2008;295:F380-7 pubmed publisher
Pacheco Otalora L, Hernandez E, Arshadmansab M, Francisco S, Willis M, Ermolinsky B, et al. Down-regulation of BK channel expression in the pilocarpine model of temporal lobe epilepsy. Brain Res. 2008;1200:116-31 pubmed publisher
Tian L, Chen L, McClafferty H, Sailer C, Ruth P, Knaus H, et al. A noncanonical SH3 domain binding motif links BK channels to the actin cytoskeleton via the SH3 adapter cortactin. FASEB J. 2006;20:2588-90 pubmed
Xu H, Jackson W, Fink G, Galligan J. Activation of potassium channels by tempol in arterial smooth muscle cells from normotensive and deoxycorticosterone acetate-salt hypertensive rats. Hypertension. 2006;48:1080-7 pubmed
Misonou H, Menegola M, Buchwalder L, Park E, Meredith A, Rhodes K, et al. Immunolocalization of the Ca2+-activated K+ channel Slo1 in axons and nerve terminals of mammalian brain and cultured neurons. J Comp Neurol. 2006;496:289-302 pubmed
product information
TargetType :
K+ channels
Target :
Slo1/BKAlpha maxi-K+ channel
AccessionNum :
Q08460-2
Clone :
L6/60
HumanGeneName :
KCNMA1
DataSheetFileName :
neuromab.ucdavis.edu/datasheet/L6_60.pdf
IsoType :
IgG2a
ValidationT :
Pass
ValidationBrIB :
Pass
ValidationBrIHC :
Pass
ValidationKO :
Pass
Catalog :
73-022
more info or order :
company information
Neuromab
PO Box 1560 Davis, CA 95617-1560
http://neuromab.ucdavis.edu
headquarters: United States