product summary
company name :
Neuromab
product type :
antibody
product name :
Kv2.1 K+ channel
catalog :
73-014
quantity :
5 ml
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
K89/34
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section, western blot knockout validation
more info or order :
citations: 87
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; rat; fig 1c
Kirmiz M, Palacio S, Thapa P, King A, Sack J, Trimmer J. Remodeling neuronal ER-PM junctions is a conserved nonconducting function of Kv2 plasma membrane ion channels. Mol Biol Cell. 2018;29:2410-2432 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 2a
Bishop H, Cobb M, Kirmiz M, Parajuli L, Mandikian D, Philp A, et al. Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons. Front Mol Neurosci. 2018;11:1 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; fig 2b
  • western blot; mouse; fig 3a
Yu W, Parakramaweera R, Teng S, Gowda M, Sharad Y, Thakker Varia S, et al. Oxidation of KCNB1 Potassium Channels Causes Neurotoxicity and Cognitive Impairment in a Mouse Model of Traumatic Brain Injury. J Neurosci. 2016;36:11084-11096 pubmed
  • immunohistochemistry; mouse; 1:250; fig 1
Lasiene J, Komine O, Fujimori Tonou N, Powers B, Endo F, Watanabe S, et al. Neuregulin 1 confers neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons. Acta Neuropathol Commun. 2016;4:15 pubmed publisher
  • western blot; mouse; fig 2
Bishop H, Guan D, Bocksteins E, Parajuli L, Murray K, Cobb M, et al. Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons. J Neurosci. 2015;35:14922-42 pubmed publisher
  • western blot; mouse; fig 4
Vullhorst D, Mitchell R, Keating C, Roychowdhury S, Karavanova I, Tao Cheng J, et al. A negative feedback loop controls NMDA receptor function in cortical interneurons via neuregulin 2/ErbB4 signalling. Nat Commun. 2015;6:7222 pubmed publisher
  • western blot; mouse; 1:2
Nieves Cintrón M, Nystoriak M, Prada M, Johnson K, Fayer W, Dell Acqua M, et al. Selective down-regulation of KV2.1 function contributes to enhanced arterial tone during diabetes. J Biol Chem. 2015;290:7918-29 pubmed publisher
  • western blot; human; 1:500
Torkamani A, Bersell K, Jorge B, Bjork R, Friedman J, Bloss C, et al. De novo KCNB1 mutations in epileptic encephalopathy. Ann Neurol. 2014;76:529-540 pubmed publisher
  • immunohistochemistry - free floating section; mouse; fig 1
Mandikian D, Bocksteins E, Parajuli L, Bishop H, Cerda O, Shigemoto R, et al. Cell type-specific spatial and functional coupling between mammalian brain Kv2.1 K+ channels and ryanodine receptors. J Comp Neurol. 2014;522:3555-74 pubmed publisher
Thiebaud N, Gribble F, Reimann F, Trapp S, Fadool D. A unique olfactory bulb microcircuit driven by neurons expressing the precursor to glucagon-like peptide 1. Sci Rep. 2019;9:15542 pubmed publisher
Nishino K, Watanabe S, Shijie J, Murata Y, Oiwa K, Komine O, et al. Mice deficient in the C-terminal domain of TAR DNA-binding protein 43 develop age-dependent motor dysfunction associated with impaired Notch1-Akt signaling pathway. Acta Neuropathol Commun. 2019;7:118 pubmed publisher
Wu Y, Fortin D, Cochrane V, Chen P, Shyng S. NMDA receptors mediate leptin signaling and regulate potassium channel trafficking in pancreatic β-cells. J Biol Chem. 2017;292:15512-15524 pubmed publisher
Frazzini V, Guarnieri S, Bomba M, Navarra R, Morabito C, Mariggiò M, et al. Altered Kv2.1 functioning promotes increased excitability in hippocampal neurons of an Alzheimer's disease mouse model. Cell Death Dis. 2016;7:e2100 pubmed publisher
Thiffault I, Speca D, Austin D, Cobb M, Eum K, Safina N, et al. A novel epileptic encephalopathy mutation in KCNB1 disrupts Kv2.1 ion selectivity, expression, and localization. J Gen Physiol. 2015;146:399-410 pubmed publisher
Wu Y, Shyng S, Chen P. Concerted Trafficking Regulation of Kv2.1 and KATP Channels by Leptin in Pancreatic β-Cells. J Biol Chem. 2015;290:29676-90 pubmed publisher
Cobb M, Austin D, Sack J, Trimmer J. Cell Cycle-dependent Changes in Localization and Phosphorylation of the Plasma Membrane Kv2.1 K+ Channel Impact Endoplasmic Reticulum Membrane Contact Sites in COS-1 Cells. J Biol Chem. 2015;290:29189-201 pubmed publisher
Peltola M, Kuja Panula J, Liuhanen J, Võikar V, Piepponen P, Hiekkalinna T, et al. AMIGO-Kv2.1 Potassium Channel Complex Is Associated With Schizophrenia-Related Phenotypes. Schizophr Bull. 2016;42:191-201 pubmed publisher
El Gebeily G, El Khoury N, Mathieu S, Brouillette J, Fiset C. Estrogen regulation of the transient outward K(+) current involves estrogen receptor α in mouse heart. J Mol Cell Cardiol. 2015;86:85-94 pubmed publisher
He K, McCord M, Hartnett K, Aizenman E. Regulation of Pro-Apoptotic Phosphorylation of Kv2.1 K+ Channels. PLoS ONE. 2015;10:e0129498 pubmed publisher
Fox P, Haberkorn C, Akin E, Seel P, Krapf D, Tamkun M. Induction of stable ER-plasma-membrane junctions by Kv2.1 potassium channels. J Cell Sci. 2015;128:2096-105 pubmed publisher
Moutal A, François Moutal L, Brittain J, Khanna M, Khanna R. Differential neuroprotective potential of CRMP2 peptide aptamers conjugated to cationic, hydrophobic, and amphipathic cell penetrating peptides. Front Cell Neurosci. 2014;8:471 pubmed publisher
Liu J, Magri L, Zhang F, Marsh N, Albrecht S, Huynh J, et al. Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation. J Neurosci. 2015;35:352-65 pubmed publisher
Ednie A, Bennett E. Reduced sialylation impacts ventricular repolarization by modulating specific K+ channel isoforms distinctly. J Biol Chem. 2015;290:2769-83 pubmed publisher
Krasowska E, Zabłocki K, Górecki D, Swinny J. Aberrant location of inhibitory synaptic marker proteins in the hippocampus of dystrophin-deficient mice: implications for cognitive impairment in duchenne muscular dystrophy. PLoS ONE. 2014;9:e108364 pubmed publisher
Siddoway B, Hou H, Yang J, Sun L, Yang H, Wang G, et al. Potassium channel Kv2.1 is regulated through protein phosphatase-1 in response to increases in synaptic activity. Neurosci Lett. 2014;583:142-7 pubmed publisher
Jensen C, Misonou H. Live-cell imaging of post-golgi transport vesicles in cultured hippocampal neurons. Cell Mol Neurobiol. 2015;35:123-35 pubmed publisher
McCord M, Kullmann P, He K, Hartnett K, Horn J, Lotan I, et al. Syntaxin-binding domain of Kv2.1 is essential for the expression of apoptotic K+ currents. J Physiol. 2014;592:3511-21 pubmed publisher
Dufour M, Woodhouse A, Goaillard J. Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development. J Neurosci Res. 2014;92:981-99 pubmed publisher
Shah N, Schulien A, Clemens K, Aizenman T, Hageman T, Wills Z, et al. Cyclin e1 regulates Kv2.1 channel phosphorylation and localization in neuronal ischemia. J Neurosci. 2014;34:4326-31 pubmed publisher
Kirizs T, Kerti Szigeti K, Lorincz A, Nusser Z. Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells. Eur J Neurosci. 2014;39:1771-83 pubmed publisher
Wang C, Huang A, Zhou M, Mei Y. GDF15 regulates Kv2.1-mediated outward K+ current through the Akt/mTOR signalling pathway in rat cerebellar granule cells. Biochem J. 2014;460:35-47 pubmed publisher
Jensen C, Watanabe S, Rasmussen H, Schmitt N, Olesen S, Frost N, et al. Specific sorting and post-Golgi trafficking of dendritic potassium channels in living neurons. J Biol Chem. 2014;289:10566-81 pubmed publisher
Speca D, Ogata G, Mandikian D, Bishop H, Wiler S, Eum K, et al. Deletion of the Kv2.1 delayed rectifier potassium channel leads to neuronal and behavioral hyperexcitability. Genes Brain Behav. 2014;13:394-408 pubmed publisher
King A, Manning C, Trimmer J. A unique ion channel clustering domain on the axon initial segment of mammalian neurons. J Comp Neurol. 2014;522:2594-608 pubmed publisher
Hund T, Snyder J, Wu X, Glynn P, Koval O, Onal B, et al. ?(IV)-Spectrin regulates TREK-1 membrane targeting in the heart. Cardiovasc Res. 2014;102:166-75 pubmed publisher
Maguire E, Macpherson T, Swinny J, Dixon C, Herd M, Belelli D, et al. Tonic inhibition of accumbal spiny neurons by extrasynaptic ?4?? GABAA receptors modulates the actions of psychostimulants. J Neurosci. 2014;34:823-38 pubmed publisher
Lopez Izquierdo A, Pereira R, Wende A, Punske B, Abel E, Tristani Firouzi M. The absence of insulin signaling in the heart induces changes in potassium channel expression and ventricular repolarization. Am J Physiol Heart Circ Physiol. 2014;306:H747-54 pubmed publisher
Cservenák M, Szabó Ã, Bodnár I, Lékó A, Palkovits M, Nagy G, et al. Thalamic neuropeptide mediating the effects of nursing on lactation and maternal motivation. Psychoneuroendocrinology. 2013;38:3070-84 pubmed publisher
Shepherd A, Loo L, Mohapatra D. Chemokine co-receptor CCR5/CXCR4-dependent modulation of Kv2.1 channel confers acute neuroprotection to HIV-1 glycoprotein gp120 exposure. PLoS ONE. 2013;8:e76698 pubmed publisher
McCord M, Aizenman E. Convergent Ca2+ and Zn2+ signaling regulates apoptotic Kv2.1 K+ currents. Proc Natl Acad Sci U S A. 2013;110:13988-93 pubmed publisher
Guan D, Armstrong W, FOEHRING R. Kv2 channels regulate firing rate in pyramidal neurons from rat sensorimotor cortex. J Physiol. 2013;591:4807-25 pubmed publisher
Amako Y, Iglói Z, Mankouri J, Kazlauskas A, Saksela K, Dallas M, et al. Hepatitis C virus NS5A inhibits mixed lineage kinase 3 to block apoptosis. J Biol Chem. 2013;288:24753-63 pubmed publisher
Mavlyutov T, Epstein M, Verbny Y, Huerta M, Zaitoun I, Ziskind Conhaim L, et al. Lack of sigma-1 receptor exacerbates ALS progression in mice. Neuroscience. 2013;240:129-34 pubmed publisher
Pirger Z, Battonyai I, Krajcs N, Elekes K, Kiss T. Voltage-gated membrane currents in neurons involved in odor information processing in snail procerebrum. Brain Struct Funct. 2014;219:673-82 pubmed publisher
Kourrich S, Hayashi T, Chuang J, Tsai S, SU T, Bonci A. Dynamic interaction between sigma-1 receptor and Kv1.2 shapes neuronal and behavioral responses to cocaine. Cell. 2013;152:236-47 pubmed publisher
Fox P, Loftus R, Tamkun M. Regulation of Kv2.1 K(+) conductance by cell surface channel density. J Neurosci. 2013;33:1259-70 pubmed publisher
Shepherd A, Loo L, Gupte R, Mickle A, Mohapatra D. Distinct modifications in Kv2.1 channel via chemokine receptor CXCR4 regulate neuronal survival-death dynamics. J Neurosci. 2012;32:17725-39 pubmed publisher
Tsantoulas C, Zhu L, Shaifta Y, Grist J, Ward J, Raouf R, et al. Sensory neuron downregulation of the Kv9.1 potassium channel subunit mediates neuropathic pain following nerve injury. J Neurosci. 2012;32:17502-13 pubmed publisher
Smith K, Wilkie S, Tebbs Warner J, Jarvis B, Gallasch L, Stocker M, et al. Functional analysis of missense mutations in Kv8.2 causing cone dystrophy with supernormal rod electroretinogram. J Biol Chem. 2012;287:43972-83 pubmed publisher
Norris C, He K, Springer M, Hartnett K, Horn J, Aizenman E. Regulation of neuronal proapoptotic potassium currents by the hepatitis C virus nonstructural protein 5A. J Neurosci. 2012;32:8865-70 pubmed publisher
Manning C, Bundros A, Trimmer J. Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance. PLoS ONE. 2012;7:e38313 pubmed publisher
Menegola M, Clark E, Trimmer J. The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice. Epilepsia. 2012;53 Suppl 1:142-9 pubmed publisher
Al Owais M, Scragg J, Dallas M, Boycott H, Warburton P, Chakrabarty A, et al. Carbon monoxide mediates the anti-apoptotic effects of heme oxygenase-1 in medulloblastoma DAOY cells via K+ channel inhibition. J Biol Chem. 2012;287:24754-64 pubmed publisher
Kinoshita M, Matsuoka Y, Suzuki T, Mirrielees J, Yang J. Sigma-1 receptor alters the kinetics of Kv1.3 voltage gated potassium channels but not the sensitivity to receptor ligands. Brain Res. 2012;1452:1-9 pubmed publisher
Dai X, Manning Fox J, Chikvashvili D, Casimir M, Plummer G, Hajmrle C, et al. The voltage-dependent potassium channel subunit Kv2.1 regulates insulin secretion from rodent and human islets independently of its electrical function. Diabetologia. 2012;55:1709-20 pubmed publisher
Zhou M, Yang G, Jiao S, Hu C, Mei Y. Cholesterol enhances neuron susceptibility to apoptotic stimuli via cAMP/PKA/CREB-dependent up-regulation of Kv2.1. J Neurochem. 2012;120:502-14 pubmed publisher
Song M, Hong C, Bae S, So I, Park K. Dynamic modulation of the kv2.1 channel by SRC-dependent tyrosine phosphorylation. J Proteome Res. 2012;11:1018-26 pubmed publisher
Peltola M, Kuja Panula J, Lauri S, Taira T, Rauvala H. AMIGO is an auxiliary subunit of the Kv2.1 potassium channel. EMBO Rep. 2011;12:1293-9 pubmed publisher
Ikematsu N, Dallas M, Ross F, Lewis R, Rafferty J, David J, et al. Phosphorylation of the voltage-gated potassium channel Kv2.1 by AMP-activated protein kinase regulates membrane excitability. Proc Natl Acad Sci U S A. 2011;108:18132-7 pubmed publisher
Joseph B, Thakali K, Pathan A, Kang E, Rusch N, Rhee S. Postsynaptic density-95 scaffolding of Shaker-type K? channels in smooth muscle cells regulates the diameter of cerebral arteries. J Physiol. 2011;589:5143-52 pubmed publisher
Brittain J, Chen L, Wilson S, Brustovetsky T, Gao X, Ashpole N, et al. Neuroprotection against traumatic brain injury by a peptide derived from the collapsin response mediator protein 2 (CRMP2). J Biol Chem. 2011;286:37778-92 pubmed publisher
Cerda O, Trimmer J. Activity-dependent phosphorylation of neuronal Kv2.1 potassium channels by CDK5. J Biol Chem. 2011;286:28738-48 pubmed publisher
Mihelec M, Pearson R, Robbie S, Buch P, Azam S, Bainbridge J, et al. Long-term preservation of cones and improvement in visual function following gene therapy in a mouse model of leber congenital amaurosis caused by guanylate cyclase-1 deficiency. Hum Gene Ther. 2011;22:1179-90 pubmed publisher
Plant L, Dowdell E, Dementieva I, Marks J, Goldstein S. SUMO modification of cell surface Kv2.1 potassium channels regulates the activity of rat hippocampal neurons. J Gen Physiol. 2011;137:441-54 pubmed publisher
Guan D, Horton L, Armstrong W, FOEHRING R. Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons. J Neurophysiol. 2011;105:2976-88 pubmed publisher
Zhuang J, Wang C, Zhou M, Duan K, Mei Y. TGF-?1 enhances Kv2.1 potassium channel protein expression and promotes maturation of cerebellar granule neurons. J Cell Physiol. 2012;227:297-307 pubmed publisher
Dallas M, Boyle J, Milligan C, Sayer R, Kerrigan T, McKinstry C, et al. Carbon monoxide protects against oxidant-induced apoptosis via inhibition of Kv2.1. FASEB J. 2011;25:1519-30 pubmed publisher
Schwetz T, Norring S, Ednie A, Bennett E. Sialic acids attached to O-glycans modulate voltage-gated potassium channel gating. J Biol Chem. 2011;286:4123-32 pubmed publisher
Song M, Moon Y, Shin S, Kim T, Yune T, Park K. Contribution of the delayed-rectifier potassium channel Kv2.1 to acute spinal cord injury in rats. BMB Rep. 2010;43:756-60 pubmed publisher
Nataraj K, Le Roux N, Nahmani M, Lefort S, Turrigiano G. Visual deprivation suppresses L5 pyramidal neuron excitability by preventing the induction of intrinsic plasticity. Neuron. 2010;68:750-62 pubmed publisher
Yang K, Foeger N, Marionneau C, Jay P, McMullen J, Nerbonne J. Homeostatic regulation of electrical excitability in physiological cardiac hypertrophy. J Physiol. 2010;588:5015-32 pubmed publisher
Hermanstyne T, Kihira Y, Misono K, Deitchler A, Yanagawa Y, Misonou H. Immunolocalization of the voltage-gated potassium channel Kv2.2 in GABAergic neurons in the basal forebrain of rats and mice. J Comp Neurol. 2010;518:4298-310 pubmed publisher
Kihira Y, Hermanstyne T, Misonou H. Formation of heteromeric Kv2 channels in mammalian brain neurons. J Biol Chem. 2010;285:15048-55 pubmed publisher
Mavlyutov T, Epstein M, Andersen K, Ziskind Conhaim L, Ruoho A. The sigma-1 receptor is enriched in postsynaptic sites of C-terminals in mouse motoneurons. An anatomical and behavioral study. Neuroscience. 2010;167:247-55 pubmed publisher
Aras M, Saadi R, Aizenman E. Zn2+ regulates Kv2.1 voltage-dependent gating and localization following ischemia. Eur J Neurosci. 2009;30:2250-7 pubmed publisher
Ito T, Nuriya M, Yasui M. Regulation of Kv2.1 phosphorylation in an animal model of anoxia. Neurobiol Dis. 2010;38:85-91 pubmed publisher
Tozakidou M, Goltz D, Hagenström T, Budack M, Vitzthum H, Szlachta K, et al. Molecular and functional remodeling of I(to) by angiotensin II in the mouse left ventricle. J Mol Cell Cardiol. 2010;48:140-51 pubmed publisher
Mankouri J, Dallas M, Hughes M, Griffin S, Macdonald A, Peers C, et al. Suppression of a pro-apoptotic K+ channel as a mechanism for hepatitis C virus persistence. Proc Natl Acad Sci U S A. 2009;106:15903-8 pubmed publisher
Eyre M, Kerti K, Nusser Z. Molecular diversity of deep short-axon cells of the rat main olfactory bulb. Eur J Neurosci. 2009;29:1397-407 pubmed publisher
Mulholland P, Carpenter Hyland E, Woodward J, Chandler L. Ethanol disrupts NMDA receptor and astroglial EAAT2 modulation of Kv2.1 potassium channels in hippocampus. Alcohol. 2009;43:45-50 pubmed publisher
Mulholland P, Carpenter Hyland E, Hearing M, Becker H, Woodward J, Chandler L. Glutamate transporters regulate extrasynaptic NMDA receptor modulation of Kv2.1 potassium channels. J Neurosci. 2008;28:8801-9 pubmed publisher
Misonou H, Thompson S, Cai X. Dynamic regulation of the Kv2.1 voltage-gated potassium channel during brain ischemia through neuroglial interaction. J Neurosci. 2008;28:8529-38 pubmed publisher
Mohapatra D, Siino D, Trimmer J. Interdomain cytoplasmic interactions govern the intracellular trafficking, gating, and modulation of the Kv2.1 channel. J Neurosci. 2008;28:4982-94 pubmed publisher
Micheva K, Smith S. Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits. Neuron. 2007;55:25-36 pubmed
Guan D, Tkatch T, Surmeier D, Armstrong W, Foehring R. Kv2 subunits underlie slowly inactivating potassium current in rat neocortical pyramidal neurons. J Physiol. 2007;581:941-60 pubmed
Misonou H, Menegola M, Mohapatra D, Guy L, Park K, Trimmer J. Bidirectional activity-dependent regulation of neuronal ion channel phosphorylation. J Neurosci. 2006;26:13505-14 pubmed
Pott C, Ren X, Tran D, Yang M, Henderson S, Jordan M, et al. Mechanism of shortened action potential duration in Na+-Ca2+ exchanger knockout mice. Am J Physiol Cell Physiol. 2007;292:C968-73 pubmed
product information
TargetType :
K+ channels
Target :
Kv2.1 K+ channel
AccessionNum :
P15387
Clone :
K89/34
HumanGeneName :
KCNB1
DataSheetFileName :
neuromab.ucdavis.edu/datasheet/K89_34.pdf
IsoType :
IgG1
ValidationT :
Pass
ValidationBrIB :
Pass
ValidationBrIHC :
Pass
ValidationKO :
Pass
Catalog :
73-014
more info or order :
company information
Neuromab
PO Box 1560 Davis, CA 95617-1560
http://neuromab.ucdavis.edu
headquarters: United States