protein

Recombinant human Ubiquitin/RPS27A protein

UBC3002

20 ug

55 USD

Recombinant human Ubiquitin/RPS27A protein

1-76aa

UBC3002

CAA44911

ubiquitin is a small protein that is composed of 76 amino acids and its sequence is highly conserved throughout evolution from invertebrates to mammals. In the cytoplasm, ubiquitin is involved in ATP-dependent nonlysomal proteolysis of various proteins. In the nucleus, ubiquitin is conjugated to histone2A and may play a role in regulation of chromatin structure and/or regulation of transcriptional activity. ubiquitin (76amino acid) was overexpressed in E. coli and purified by using conventional chromatography techniques.

Ribosomal protein S27a, Ubiquitin carboxyl extension protein 80, Small ribosomal subunit protein eS31, UBA80, UBCEP1, UBCEP80,

1mg/ml (determined by BCA assay)

Liquid in. 50mM HEPES buffer (pH 7.4) containing 150mM NaCl, 1mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lund PK., et al. (1985) J Biol. Chem. 260(12), 7609-7613.;; Pancre V., et al. (1991) Eur J Immunol. 21(11):2735-41.

> 95% by SDS-PAGE

SDS-PAGE

Human

Ubiquitination

8.6 kDa (76aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Non-Tagged

E.coli

Recombinant Protein

P62979

ubiquitin, RPS27A, uBA80, uBCEP1, uBA52, uBCEP2, uBB, uBC, ubiquitin, FLJ25987, MGC8385, Polyubiquitin B, ubiquitin B,UBC3002-10ug,UBC3002-20ug,UBC3002-50ug,UBC3002-100ug,UBC3002-250ug,UBC3002-500ug,UBC3002-1mg,UBC3002-10,UBC3002-20,UBC3002-50,UBC3002-100,UBC3002-250,UBC3002-500,UBC3002-1

Jeong WJ, et al. Ras stabilization through aberrant activation of Wnt/β-catenin signaling promotes intestinal tumorigenesis. (Sci Signal. 2012){https://pubmed.ncbi.nlm.nih.gov/22494971/}